Rw5G043900

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001224
Physical Location & Seq
Forward (+)
77045918 .. 77047661
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043900.1

Sequence Viewer

Length: 1560 bp
ATGTCTTCCTCATCAAATGCTTTCCAATGGTTAAGCCTAGTGGCCATCATATGGCTTCAGTCCATAAATGGAACAAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTATCCTTGTCCCAACTACAAATCAACAATCTCGTCTTTGCCTCCGACGCAGGGAAGCTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCGAGTCCTCATGATCGGCTCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAGACAAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCCGGAAATAGCGTCTGTTGGATCAACACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTATCAAGGATTGAGTGCTAAAATCTACACAGATATTGTTAGTACTGCCTTTTCTTCTTCACCTCATAAAAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCGAGAAACATGGCAGCGGGGTTCAATTTGATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAATGATAGGTACCGGAGTGTTCCTACTGATCCCCTTGGTAATCCCAATTGTAGTAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAAAGTGGTGTAGTTAAACAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATGATGAGGTTGGTGAGGTACAGGTTAATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTGTATTTTTTCAGTGCAACAATTGGTCTTGTGTTCTTGAACACCTTGGGACAGATAGCGGAGTCAAGGGGTTCATCTAAGACTTCTTCTCTGGTTTCTTTATCCTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGACTATTTCTTCTCAAGCAGCACCATGGCAGGAGTTTTTTTTTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAACTATTCGGGACCAAGAAATTCTCAATAAATCACAATGTGGTGGTTGCGAATATCTCATTTGGGTCGTTTCTTTTTGGCTACTCGTCTGCTCTTCTTTATCGCAAGGAAGGAGATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTACAGAAGCACCTTCATTATCTGGGGTAGTCTCTGTACGTTAGGGACTGTCCTAGCTTTAACGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

519

Amino Acids

56.95

Weight (kDa)

8.56

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 256 2.4e-72 Nodulin-like
NFD4_C PF23262 318 - 516 4.4e-90 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 568, 719
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 3 cut(s) 623, 1079, 1251
AclWI GGATC 3 cut(s) 368, 697, 733
AcoI YGGCCR 3 cut(s) 42, 339, 1144
AcsI RAATTY 1 cut(s) 1328
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1348
AfaI GTAC 7 cut(s) 481, 721, 821, 904, 936, 1249, 1492
AfiI CCNNNNNNNGG 2 cut(s) 51, 168
AgsI TTSAA 2 cut(s) 631, 1060
AhlI ACTAGT 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 4 cut(s) 175, 1226, 1437, 1511
AluI AGCT 4 cut(s) 175, 1226, 1437, 1511
Alw21I GWGCWC 2 cut(s) 514, 1271
Alw26I GTCTC 3 cut(s) 1154, 1237, 1490
Alw44I GTGCAC 1 cut(s) 1267
AlwI GGATC 3 cut(s) 368, 697, 733
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 42, 339, 516, 847, 1144
ApaLI GTGCAC 1 cut(s) 1267
ApeKI GCWGC 4 cut(s) 200, 620, 1185, 1437
ApoI RAATTY 1 cut(s) 1328
Asp718I GGTACC 1 cut(s) 719
AspS9I GGNCC 1 cut(s) 1320
AsuHPI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 1320
BaeGI GKGCMC 1 cut(s) 1271
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 2 cut(s) 568, 719
Bbv12I GWGCWC 2 cut(s) 514, 1271
BbvI GCAGC 4 cut(s) 187, 632, 1197, 1424
BccI CCATC 4 cut(s) 53, 786, 979, 1424
BceAI ACGGC 3 cut(s) 280, 656, 1131
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 1154, 1237, 1490
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 5 cut(s) 38, 551, 800, 1019, 1508
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 4 cut(s) 201, 621, 1186, 1438
BlsI GCNGC 4 cut(s) 202, 622, 1187, 1439
BmcAI AGTACT 1 cut(s) 481
Bme18I GGWCC 1 cut(s) 1320
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 1320
BmiI GGNNCC 5 cut(s) 570, 606, 721, 797, 1321
BpmI CTGGAG 2 cut(s) 288, 1293
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 3 cut(s) 92, 872, 1165
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 333, 744, 1065, 1191
BsaWI WCCGGW 2 cut(s) 659, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 168
Bse1I ACTGG 2 cut(s) 681, 1276
Bse3DI GCAATG 2 cut(s) 195, 651
BseDI CCNNGG 4 cut(s) 333, 744, 1065, 1191
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 51, 168
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 681, 1276
BseRI GAGGAG 1 cut(s) 980
BseSI GKGCMC 1 cut(s) 1271
BseXI GCAGC 4 cut(s) 187, 632, 1197, 1424
BseYI CCCAGC 1 cut(s) 1222
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BshNI GGYRCC 2 cut(s) 568, 719
BsiHKAI GWGCWC 2 cut(s) 514, 1271
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 192, 342, 660, 723
BslFI GGGAC 5 cut(s) 112, 438, 1083, 1333, 1513
BslI CCNNNNNNNGG 2 cut(s) 51, 168
BsmAI GTCTC 3 cut(s) 1154, 1237, 1490
BsmBI CGTCTC 2 cut(s) 1154, 1237
BsmFI GGGAC 5 cut(s) 112, 438, 1083, 1333, 1513
BsnI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 514, 1271
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
Bsp19I CCATGG 2 cut(s) 333, 1191
BspACI CCGC 3 cut(s) 623, 1079, 1251
BspANI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BspHI TCATGA 2 cut(s) 231, 916
BspLI GGNNCC 5 cut(s) 570, 606, 721, 797, 1321
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 3 cut(s) 368, 697, 733
BspQI GCTCTTC 1 cut(s) 1407
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 2 cut(s) 568, 719
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1276
BssECI CCNNGG 4 cut(s) 333, 744, 1065, 1191
BssMI GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 4 cut(s) 333, 744, 1065, 1191
Bst4CI ACNGT 3 cut(s) 370, 907, 1504
Bst6I CTCTTC 3 cut(s) 885, 1132, 1407
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstDEI CTNAG 2 cut(s) 703, 1098
BstDSI CCRYGG 2 cut(s) 333, 1191
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 7 cut(s) 237, 363, 692, 699, 741, 841, 1556
BstMAI GTCTC 3 cut(s) 1154, 1237, 1490
BstMBI GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
BstMWI GCNNNNNNNGC 2 cut(s) 164, 855
BstSLI GKGCMC 1 cut(s) 1271
BstV1I GCAGC 4 cut(s) 187, 632, 1197, 1424
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BtgI CCRYGG 2 cut(s) 333, 1191
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 1299
BtsIMutI CAGTG 4 cut(s) 912, 1039, 1269, 1299
BveI ACCTGC 1 cut(s) 901
CciI TCATGA 2 cut(s) 231, 916
Cfr13I GGNCC 1 cut(s) 1320
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 7 cut(s) 480, 720, 820, 903, 935, 1248, 1491
CviAII CATG 7 cut(s) 232, 334, 616, 917, 947, 1153, 1192
CviQI GTAC 7 cut(s) 480, 720, 820, 903, 935, 1248, 1491
DdeI CTNAG 2 cut(s) 703, 1098
DpnI GATC 7 cut(s) 236, 362, 691, 698, 740, 840, 1555
DpnII GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
DraIII CACNNNGTG 1 cut(s) 1348
EaeI YGGCCR 3 cut(s) 42, 339, 1144
Eam1104I CTCTTC 3 cut(s) 885, 1132, 1407
EarI CTCTTC 3 cut(s) 885, 1132, 1407
Eco130I CCWWGG 4 cut(s) 333, 744, 1065, 1191
Eco147I AGGCCT 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 1320
Eco57I CTGAAG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 4 cut(s) 333, 744, 1065, 1191
ErhI CCWWGG 4 cut(s) 333, 744, 1065, 1191
Esp3I CGTCTC 2 cut(s) 1154, 1237
FaeI CATG 7 cut(s) 235, 337, 619, 920, 950, 1156, 1195
FaqI GGGAC 5 cut(s) 112, 438, 1083, 1333, 1513
FatI CATG 7 cut(s) 231, 333, 615, 916, 946, 1152, 1191
FauI CCCGC 1 cut(s) 616
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 201, 621, 1186, 1438
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 4 cut(s) 201, 621, 1186, 1438
FspBI CTAG 5 cut(s) 38, 551, 800, 1019, 1508
GluI GCNGC 4 cut(s) 201, 621, 1186, 1438
GsaI CCCAGC 1 cut(s) 1226
GsuI CTGGAG 2 cut(s) 288, 1293
HaeIII GGCC 5 cut(s) 44, 341, 518, 849, 1146
HapII CCGG 4 cut(s) 192, 342, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 7 cut(s) 235, 337, 619, 920, 950, 1156, 1195
HinfI GANTC 3 cut(s) 225, 663, 1082
HpaII CCGG 4 cut(s) 192, 342, 660, 723
HphI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
Hpy166II GTNNAC 2 cut(s) 820, 1269
Hpy188I TCNGA 3 cut(s) 163, 773, 843
Hpy188III TCNNGA 7 cut(s) 109, 232, 786, 917, 1057, 1166, 1318
Hpy8I GTNNAC 2 cut(s) 820, 1269
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 6 cut(s) 94, 221, 1141, 1167, 1412, 1477
HpyCH4III ACNGT 3 cut(s) 370, 907, 1504
HpyCH4IV ACGT 2 cut(s) 1230, 1493
HpyCH4V TGCA 6 cut(s) 910, 991, 1037, 1269, 1292, 1526
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 855
HpyF3I CTNAG 2 cut(s) 703, 1098
HpySE526I ACGT 2 cut(s) 1230, 1493
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 7 cut(s) 235, 337, 619, 920, 950, 1156, 1195
KpnI GGTACC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
LguI GCTCTTC 1 cut(s) 1407
LmnI GCTCC 3 cut(s) 604, 795, 1274
Lsp1109I GCAGC 4 cut(s) 187, 632, 1197, 1424
MaeI CTAG 5 cut(s) 38, 551, 800, 1019, 1508
MaeII ACGT 2 cut(s) 1230, 1493
MaeIII GTNAC 2 cut(s) 283, 1454
MalI GATC 7 cut(s) 236, 362, 691, 698, 740, 840, 1555
MboI GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
MfeI CAATTG 2 cut(s) 756, 1041
MhlI GDGCHC 2 cut(s) 514, 1271
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 6 cut(s) 272, 631, 756, 1041, 1278, 1328
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 234, 1091
MmeI TCCRAC 2 cut(s) 186, 338
MnlI CCTC 9 cut(s) 19, 169, 239, 510, 886, 916, 925, 958, 1135
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 4 cut(s) 32, 876, 942, 1514
MslI CAYNNNNRTG 2 cut(s) 335, 590
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 192, 342, 660, 723
MunI CAATTG 2 cut(s) 756, 1041
MwoI GCNNNNNNNGC 2 cut(s) 164, 855
NcoI CCATGG 2 cut(s) 333, 1191
NdeI CATATG 1 cut(s) 50
NdeII GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
NlaIII CATG 7 cut(s) 235, 337, 619, 920, 950, 1156, 1195
NlaIV GGNNCC 5 cut(s) 570, 606, 721, 797, 1321
NmeAIII GCCGAG 1 cut(s) 633
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 2 cut(s) 231, 916
PceI AGGCCT 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 1407
PfeI GAWTC 1 cut(s) 663
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 4 cut(s) 202, 622, 1187, 1439
PleI GAGTC 2 cut(s) 233, 1090
PpsI GAGTC 2 cut(s) 233, 1090
PspFI CCCAGC 1 cut(s) 1222
PspN4I GGNNCC 5 cut(s) 570, 606, 721, 797, 1321
PspPI GGNCC 1 cut(s) 1320
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 7 cut(s) 481, 721, 821, 904, 936, 1249, 1492
RsaNI GTAC 7 cut(s) 480, 720, 820, 903, 935, 1248, 1491
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1407
SaqAI TTAA 4 cut(s) 32, 876, 942, 1514
SatI GCNGC 4 cut(s) 201, 621, 1186, 1438
Sau3AI GATC 7 cut(s) 234, 360, 689, 696, 738, 838, 1553
Sau96I GGNCC 1 cut(s) 1320
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 2 cut(s) 234, 1091
SduI GDGCHC 2 cut(s) 514, 1271
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 1 cut(s) 1320
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 3 cut(s) 107, 851, 1180
SmoI CTYRAG 3 cut(s) 107, 851, 1180
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 6 cut(s) 272, 631, 756, 1041, 1278, 1328
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 3 cut(s) 623, 1079, 1251
SspI AATATT 1 cut(s) 321
SspMI CTAG 5 cut(s) 38, 551, 800, 1019, 1508
StuI AGGCCT 1 cut(s) 518
StyI CCWWGG 4 cut(s) 333, 744, 1065, 1191
TaaI ACNGT 3 cut(s) 370, 907, 1504
TaiI ACGT 2 cut(s) 1233, 1496
TaqI TCGA 1 cut(s) 391
TasI AATT 6 cut(s) 272, 631, 756, 1041, 1278, 1328
TatI WGTACW 3 cut(s) 479, 819, 902
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 4 cut(s) 32, 876, 942, 1514
Tru9I TTAA 4 cut(s) 32, 876, 942, 1514
TscAI CASTG 4 cut(s) 912, 1039, 1276, 1299
TseI GCWGC 4 cut(s) 200, 620, 1185, 1437
TspDTI ATGAA 4 cut(s) 905, 1083, 1440, 1459
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 4 cut(s) 912, 1039, 1276, 1299
Van91I CCANNNNNTGG 1 cut(s) 51
VneI GTGCAC 1 cut(s) 1267
VpaK11BI GGWCC 1 cut(s) 1320
XapI RAATTY 1 cut(s) 1328
XspI CTAG 5 cut(s) 38, 551, 800, 1019, 1508
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.