MD03G1058400.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001224
Physical Location & Seq
Forward (+)
4706855 .. 4708986
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1058400.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGCCGTCTTCTTCTTCTTCTTCTTCTTCTTCTGCCCTCCAATGGTTAAGCCTAGTGGGAATCATATGGCTTCAGTCCATCAATGGAACCAACACCAACTTCCCAGCTTACTCCTCCCAGCTCAAAAACGTCCTCTCCATGTCCCAACTAGAGCTCAACAACCTCGCGTTCGCCTCAGACGCTGGAAAATTCCTTGGTTGGTTTTCAGGCATTGCTGCCATCTACTTACCGCTCTGGCTAGTCCTCATGATCGGTTCGCTTCTCGGTCTAATCGGGTACGGTGTCCAGTTCCTCTTCATCACAAACCAAATCTCATCTCCTTCCTATTGGCAGATTTTCTTGCTAACTGTTATGGCTGGGAATAGCATTTGCTGGATCAACACTGTGTGCTATGTTGTTTGCATTAGAAACTTCCCATGTCATCAACAAATTGCCGTGGGGATAACAACGAGTTACCAAGGATTGAGTGCCAAGATTTATACAGATATTGTTGGCGCTGTATTTTCGTCTTCACCTCATAGAAGAGCCAAGGCCTACTTATTTCTCAACTCTCTTTTGCCACTTGTTGTTTGTGTTATCGTCATGCCATTGGTTCGAGACATTGATTTGATGGGAAGGCAAAGGAACATGGAAGCTGGGTTTATCTTCATGTTTGTGATAACAATTGCCACGGGAATCTACGCGGTGATCAGCAGTTTAGGGTCCACGTCTGGCGGGTTATCTCCCCTACACAATGTAATAGGCACCGGAGTATTCCTGTTAATCCCGTTGGTGATTCCAATTGTTGAGAAAATGAGGGAAATAATGTCAAGAAAATGGAACGTAAACAGAGAGAGAAGAGTATATAATTTTACCATAGAAGAGACGAACAACGAAATGGGGAGCTTGGAGAATGGGGCAGTGAAGGAGGGAGAAGAAATAGAGGCTAGCCAAGTTCATGAAGTTCGTGTTAGGGAGGAGATTGGAGTGAAGTTGATGGTGACAAGGATTGATTTTTGGTTATATTTATTTGTGTATTTTTTCAGTGCAACACTTGGGTTGGTGTTCTTGAACAACTTGGGACAGATTGCAGAGTCCAGGGGCTCATCTAGGACTTCTTCTCTAGTCTCTTTATCCTCTTCATTTGGGTTTTTTGGCCGTCTCATGCCTTCTCTTTTGGACTATTTCTTCTCAAGGAGTAAGTACATGATTTCAAGACCAGCATTAATAGTTGCATTAATGGCACCAATAGCAGGAGCTTTCTTCTTGCTTCTCAACCCAGCCAACCTCTCCCTTTTCATCAGCACAGCAATTATAGGAGTGTGTGCTGGAGCAATTACTTCAATCGCAGTGTCCATAACCACAGAGCTGTTTGGGACCAAGAATTTCTCAGTGAACCACAATGTGGTGGTGGCCAACATATCAATCGGGTCTTTTGTGTTTGGGTACATGTCTGCTATTTTTTATCACAAGGATGGAAATCATGATGGGGATGGGAAGTGCATGGGAATGGCTTGCTACAGAAGCACTTTTATTATATGGGGCTGCCTTTGTTTTCTGGGCAGTGGCCTAGCTTTAATGCTCTACGCACGAACACGAAAGTTCTACTCCCAAAGATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

534

Amino Acids

58.69

Weight (kDa)

9.01

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 14 - 261 1.5e-77 Nodulin-like
NFD4_C PF23262 316 - 530 3.9e-103 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 743, 1222
AccB7I CCANNNNNTGG 1 cut(s) 1384
AccBSI CCGCTC 1 cut(s) 232
AccII CGCG 2 cut(s) 167, 683
AciI CCGC 3 cut(s) 230, 683, 714
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 2 cut(s) 1135, 1392
AcsI RAATTY 2 cut(s) 188, 1363
AcuI CTGAAG 1 cut(s) 56
AdeI CACNNNGTG 2 cut(s) 387, 1384
AfaI GTAC 3 cut(s) 278, 1184, 1427
AfiI CCNNNNNNNGG 3 cut(s) 42, 1232, 1384
AflIII ACRYGT 1 cut(s) 1428
AgsI TTSAA 3 cut(s) 1051, 1194, 1323
AjiI CACGTC 1 cut(s) 708
AjnI CCWGG 1 cut(s) 1076
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AluBI AGCT 8 cut(s) 107, 121, 154, 635, 885, 1238, 1348, 1553
AluI AGCT 8 cut(s) 107, 121, 154, 635, 885, 1238, 1348, 1553
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 4 cut(s) 591, 857, 1111, 1145
AlwI GGATC 1 cut(s) 383
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 4 cut(s) 531, 1135, 1392, 1546
ApeKI GCWGC 2 cut(s) 215, 1524
ApoI RAATTY 2 cut(s) 188, 1363
AseI ATTAAT 2 cut(s) 1205, 1217
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 702, 1356
AsuHPI GGTGA 4 cut(s) 504, 697, 784, 991
AsuNHI GCTAGC 1 cut(s) 926
AvaII GGWCC 2 cut(s) 702, 1356
BalI TGGCCA 1 cut(s) 1394
BanI GGYRCC 2 cut(s) 743, 1222
BanII GRGCYC 2 cut(s) 156, 1085
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 202, 1511
BccI CCATC 7 cut(s) 86, 227, 604, 970, 1448, 1460, 1466
BceAI ACGGC 2 cut(s) 419, 1122
BciT130I CCWGG 1 cut(s) 1078
BclI TGATCA 1 cut(s) 687
BcoDI GTCTC 4 cut(s) 591, 857, 1111, 1145
BfaI CTAG 7 cut(s) 53, 149, 239, 927, 1089, 1103, 1550
BfmI CTRYAG 1 cut(s) 1498
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 2 cut(s) 216, 1525
BlsI GCNGC 2 cut(s) 217, 1526
Bme1390I CCNGG 1 cut(s) 1078
Bme18I GGWCC 2 cut(s) 702, 1356
BmgBI CACGTC 1 cut(s) 708
BmgT120I GGNCC 2 cut(s) 702, 1356
BmiI GGNNCC 5 cut(s) 88, 703, 745, 1224, 1357
BmrFI CCNGG 1 cut(s) 1078
BmtI GCTAGC 1 cut(s) 930
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 1329
BpuEI CTTGAG 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1458
BsaJI CCNNGG 6 cut(s) 193, 435, 457, 528, 669, 1077
BsaWI WCCGGW 1 cut(s) 746
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 1232, 1384
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 1458
BseBI CCWGG 1 cut(s) 1078
BseDI CCNNGG 6 cut(s) 193, 435, 457, 528, 669, 1077
BseGI GGATG 2 cut(s) 1459, 1477
BseJI GATNNNNATC 1 cut(s) 1458
BseLI CCNNNNNNNGG 3 cut(s) 42, 1232, 1384
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 2 cut(s) 189, 1383
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 2 cut(s) 103, 971
BseXI GCAGC 2 cut(s) 202, 1511
BseYI CCCAGC 5 cut(s) 103, 117, 356, 635, 1258
Bsh1236I CGCG 2 cut(s) 167, 683
BshFI GGCC 4 cut(s) 533, 1137, 1394, 1548
BshNI GGYRCC 2 cut(s) 743, 1222
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 1 cut(s) 747
BslFI GGGAC 3 cut(s) 127, 1074, 1369
BslI CCNNNNNNNGG 3 cut(s) 42, 1232, 1384
BsmAI GTCTC 4 cut(s) 591, 857, 1111, 1145
BsmBI CGTCTC 2 cut(s) 857, 1145
BsmFI GGGAC 3 cut(s) 127, 1074, 1369
BsnI GGCC 4 cut(s) 533, 1137, 1394, 1548
Bsp1286I GDGCHC 2 cut(s) 156, 1085
Bsp143I GATC 4 cut(s) 249, 375, 687, 1595
BspACI CCGC 3 cut(s) 230, 683, 714
BspANI GGCC 4 cut(s) 533, 1137, 1394, 1548
BspCNI CTCAG 2 cut(s) 188, 1382
BspFNI CGCG 2 cut(s) 167, 683
BspHI TCATGA 3 cut(s) 246, 937, 1462
BspLI GGNNCC 5 cut(s) 88, 703, 745, 1224, 1357
BspOI GCTAGC 1 cut(s) 930
BspPI GGATC 1 cut(s) 383
BspQI GCTCTTC 1 cut(s) 517
BspT107I GGYRCC 2 cut(s) 743, 1222
BsrBI CCGCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 210
BsrI ACTGG 1 cut(s) 286
BssECI CCNNGG 6 cut(s) 193, 435, 457, 528, 669, 1077
BssMI GATC 4 cut(s) 249, 375, 687, 1595
BssT1I CCWWGG 3 cut(s) 193, 457, 528
Bst2UI CCWGG 1 cut(s) 1078
Bst4CI ACNGT 3 cut(s) 281, 349, 385
Bst6I CTCTTC 5 cut(s) 299, 517, 832, 855, 1123
BstC8I GCNNGC 2 cut(s) 928, 1495
BstDEI CTNAG 2 cut(s) 175, 1369
BstDSI CCRYGG 2 cut(s) 435, 669
BstF5I GGATG 2 cut(s) 1459, 1477
BstFNI CGCG 2 cut(s) 167, 683
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 4 cut(s) 252, 378, 690, 1598
BstMAI GTCTC 4 cut(s) 591, 857, 1111, 1145
BstMBI GATC 4 cut(s) 249, 375, 687, 1595
BstMWI GCNNNNNNNGC 4 cut(s) 179, 1220, 1229, 1503
BstNI CCWGG 1 cut(s) 1078
BstNSI RCATGY 1 cut(s) 1432
BstSCI CCNGG 1 cut(s) 1076
BstSFI CTRYAG 1 cut(s) 1498
BstUI CGCG 2 cut(s) 167, 683
BstV1I GCAGC 2 cut(s) 202, 1511
BstV2I GAAGAC 1 cut(s) 501
BsuRI GGCC 4 cut(s) 533, 1137, 1394, 1548
BtgI CCRYGG 2 cut(s) 435, 669
BtrI CACGTC 1 cut(s) 708
BtsCI GGATG 2 cut(s) 1459, 1477
BtsI GCAGTG 3 cut(s) 906, 1335, 1549
BtsIMutI CAGTG 6 cut(s) 381, 906, 1030, 1335, 1377, 1549
Cac8I GCNNGC 2 cut(s) 928, 1495
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 3 cut(s) 246, 937, 1462
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 702, 1356
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 277, 1183, 1426
CviQI GTAC 3 cut(s) 277, 1183, 1426
DdeI CTNAG 2 cut(s) 175, 1369
DpnI GATC 4 cut(s) 251, 377, 689, 1597
DpnII GATC 4 cut(s) 249, 375, 687, 1595
DraIII CACNNNGTG 2 cut(s) 387, 1384
EaeI YGGCCR 2 cut(s) 1135, 1392
Eam1104I CTCTTC 5 cut(s) 299, 517, 832, 855, 1123
EarI CTCTTC 5 cut(s) 299, 517, 832, 855, 1123
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 3 cut(s) 193, 457, 528
Eco147I AGGCCT 1 cut(s) 533
Eco24I GRGCYC 2 cut(s) 156, 1085
Eco47I GGWCC 2 cut(s) 702, 1356
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 56
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 1076
EcoT14I CCWWGG 3 cut(s) 193, 457, 528
EcoT38I GRGCYC 2 cut(s) 156, 1085
ErhI CCWWGG 3 cut(s) 193, 457, 528
Esp3I CGTCTC 2 cut(s) 857, 1145
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 3 cut(s) 127, 1074, 1369
FauI CCCGC 1 cut(s) 707
FauNDI CATATG 1 cut(s) 65
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 2 cut(s) 216, 1525
FokI GGATG 2 cut(s) 1466, 1484
FriOI GRGCYC 2 cut(s) 156, 1085
Fsp4HI GCNGC 2 cut(s) 216, 1525
FspBI CTAG 7 cut(s) 53, 149, 239, 927, 1089, 1103, 1550
GlaI GCGC 1 cut(s) 496
GluI GCNGC 2 cut(s) 216, 1525
GsaI CCCAGC 5 cut(s) 107, 121, 360, 639, 1262
GsuI CTGGAG 1 cut(s) 1329
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 4 cut(s) 533, 1137, 1394, 1548
HapII CCGG 1 cut(s) 747
HgaI GACGC 1 cut(s) 188
HhaI GCGC 1 cut(s) 497
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HinfI GANTC 4 cut(s) 60, 675, 775, 1073
HpaII CCGG 1 cut(s) 747
HphI GGTGA 4 cut(s) 504, 697, 784, 991
Hpy166II GTNNAC 3 cut(s) 705, 826, 1375
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 7 cut(s) 247, 596, 810, 938, 1048, 1194, 1463
Hpy8I GTNNAC 3 cut(s) 705, 826, 1375
HpyAV CCTTC 4 cut(s) 330, 609, 898, 1158
HpyCH4III ACNGT 3 cut(s) 281, 349, 385
HpyCH4IV ACGT 3 cut(s) 129, 707, 822
HpyCH4V TGCA 5 cut(s) 402, 1028, 1070, 1214, 1482
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 1220, 1229, 1503
HpyF3I CTNAG 2 cut(s) 175, 1369
HpySE526I ACGT 3 cut(s) 129, 707, 822
HspAI GCGC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 4 cut(s) 249, 375, 687, 1595
LguI GCTCTTC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 882, 1235, 1310
Lsp1109I GCAGC 2 cut(s) 202, 1511
MaeI CTAG 7 cut(s) 53, 149, 239, 927, 1089, 1103, 1550
MaeII ACGT 3 cut(s) 129, 707, 822
MaeIII GTNAC 2 cut(s) 452, 979
MalI GATC 4 cut(s) 251, 377, 689, 1597
MbiI CCGCTC 1 cut(s) 232
MboI GATC 4 cut(s) 249, 375, 687, 1595
MfeI CAATTG 2 cut(s) 663, 780
MhlI GDGCHC 2 cut(s) 156, 1085
MlsI TGGCCA 1 cut(s) 1394
MluCI AATT 8 cut(s) 188, 429, 663, 780, 847, 1290, 1314, 1363
MluNI TGGCCA 1 cut(s) 1394
MlyI GAGTC 1 cut(s) 1082
Mox20I TGGCCA 1 cut(s) 1394
MscI TGGCCA 1 cut(s) 1394
MseI TTAA 5 cut(s) 47, 761, 1205, 1217, 1556
MslI CAYNNNNRTG 3 cut(s) 653, 1452, 1487
Msp20I TGGCCA 1 cut(s) 1394
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 1078
MunI CAATTG 2 cut(s) 663, 780
MvaI CCWGG 1 cut(s) 1078
MvnI CGCG 2 cut(s) 167, 683
MwoI GCNNNNNNNGC 4 cut(s) 179, 1220, 1229, 1503
NdeI CATATG 1 cut(s) 65
NdeII GATC 4 cut(s) 249, 375, 687, 1595
NheI GCTAGC 1 cut(s) 926
NlaIV GGNNCC 5 cut(s) 88, 703, 745, 1224, 1357
NmuCI GTSAC 1 cut(s) 979
NspI RCATGY 1 cut(s) 1432
PagI TCATGA 3 cut(s) 246, 937, 1462
PceI AGGCCT 1 cut(s) 533
PciI ACATGT 1 cut(s) 1428
PciSI GCTCTTC 1 cut(s) 517
PfeI GAWTC 3 cut(s) 60, 675, 775
PflMI CCANNNNNTGG 1 cut(s) 1384
PkrI GCNGC 2 cut(s) 217, 1526
PleI GAGTC 1 cut(s) 1081
PpsI GAGTC 1 cut(s) 1081
PscI ACATGT 1 cut(s) 1428
PshBI ATTAAT 2 cut(s) 1205, 1217
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 1076
PspFI CCCAGC 5 cut(s) 103, 117, 356, 635, 1258
PspGI CCWGG 1 cut(s) 1076
PspN4I GGNNCC 5 cut(s) 88, 703, 745, 1224, 1357
PspPI GGNCC 2 cut(s) 702, 1356
PstNI CAGNNNCTG 1 cut(s) 182
RsaI GTAC 3 cut(s) 278, 1184, 1427
RsaNI GTAC 3 cut(s) 277, 1183, 1426
RseI CAYNNNNRTG 3 cut(s) 653, 1452, 1487
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 517
SaqAI TTAA 5 cut(s) 47, 761, 1205, 1217, 1556
SatI GCNGC 2 cut(s) 216, 1525
Sau3AI GATC 4 cut(s) 249, 375, 687, 1595
Sau96I GGNCC 2 cut(s) 702, 1356
SchI GAGTC 1 cut(s) 1082
ScrFI CCNGG 1 cut(s) 1078
SduI GDGCHC 2 cut(s) 156, 1085
SfcI CTRYAG 1 cut(s) 1498
SinI GGWCC 2 cut(s) 702, 1356
SmiMI CAYNNNNRTG 3 cut(s) 653, 1452, 1487
SmlI CTYRAG 1 cut(s) 1171
SmoI CTYRAG 1 cut(s) 1171
Sse9I AATT 8 cut(s) 188, 429, 663, 780, 847, 1290, 1314, 1363
SseBI AGGCCT 1 cut(s) 533
SsiI CCGC 3 cut(s) 230, 683, 714
SspMI CTAG 7 cut(s) 53, 149, 239, 927, 1089, 1103, 1550
SstI GAGCTC 1 cut(s) 156
StuI AGGCCT 1 cut(s) 533
StyD4I CCNGG 1 cut(s) 1076
StyI CCWWGG 3 cut(s) 193, 457, 528
TaaI ACNGT 3 cut(s) 281, 349, 385
TaiI ACGT 3 cut(s) 132, 710, 825
TaqI TCGA 1 cut(s) 595
TaqII GACCGA 1 cut(s) 254
TasI AATT 8 cut(s) 188, 429, 663, 780, 847, 1290, 1314, 1363
TatI WGTACW 1 cut(s) 1182
TfiI GAWTC 3 cut(s) 60, 675, 775
Tru1I TTAA 5 cut(s) 47, 761, 1205, 1217, 1556
Tru9I TTAA 5 cut(s) 47, 761, 1205, 1217, 1556
TscAI CASTG 6 cut(s) 388, 906, 1030, 1335, 1377, 1549
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 2 cut(s) 215, 1524
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 6 cut(s) 286, 637, 926, 954, 1110, 1267
TspRI CASTG 6 cut(s) 388, 906, 1030, 1335, 1377, 1549
Van91I CCANNNNNTGG 1 cut(s) 1384
VpaK11BI GGWCC 2 cut(s) 702, 1356
VspI ATTAAT 2 cut(s) 1205, 1217
XapI RAATTY 2 cut(s) 188, 1363
XceI RCATGY 1 cut(s) 1432
XspI CTAG 7 cut(s) 53, 149, 239, 927, 1089, 1103, 1550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.