pycom03g04680

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001224
Physical Location & Seq
Forward (+)
3631210 .. 3633274
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04680.1

Sequence Viewer

Length: 1716 bp
ATGGACATAGAAACTCCTGTGGTACACACACTATATAAAACAGTATTGTGCATTTGCAAATCACCAACAAACATAACAGAAACCCTAATTTCATCAAAACCCACCTCATATTTCCTCATGCCGTCTTCTTCTTCTTCTTCTTCTTCTGCTCTCCAATGGTTAAGCCTAGTGGGAATCATATGGCTTCAGGCCATCAATGGAACCAACACCAACTTCCCAGCTTACTCCTCCCAGCTCAAAAACGTCCTCTCCATGTCCCAACTACAGCTCAACAACCTCGCCTTCGCCTCAGACGCTGGAAAACTCCTTGGTTGGTTTTCCGGCATTGCTGCCATCTACTTACCCCTCTGGCTAGTCCTCATGATCGGTTCGCTTCTCGGTCTAATAGGGTACGGCGTCCAGTTCCTCTTCATCACAAACCAAATCTCATCTCCTTCCTATTGGCAGATTTTCTTGCTAACTGTTATGGCTGGGAATAGCATTTGCTGGATCAACACTGTGTGCTATGTTGTTTGCATTAGAAACTTCCCATGTCATCAACAAATTGCCGTGGGGATAACAACGAGTTACCAAGGATTGAGCGCCAAGATTTATACAGATATTGTTGGCGCTGTATTTTCGTCTTCACCTCATAGAAGAGCCAAGGCCTACTTATTTCTCAACTCTCTTTTGCCACTTGTTGTTTGTGTTATCGTCATGCCATTGGTTCGAGACGTTGATTTGATGGGAAGGCAAAGGAACATGGAAGCTGGGTTTATCTTCATGTTTGTGATAACAATTGCCACCGGAATCTACGCGGTGATCAGCAGTTTAGGGTCGACTTCTGGCGGGTTATCTCCCCTACACAATTTAATAGGCACCGGATTATTCCTGTTAATCCCGTTGGTGATTCCAATTGTTGAGAAAATGAGGGAAATAATGTCAAGAAAATGGAACGTAAACAGAGAGAGAAGAGTATATAATTTTACCATAGAAGAGAAGAACAACGAAATGGGGAGCTTGGAGAATGGGGGAGTGAAGGAGGGAGAAGAAATAGAGGCTAGGCAAGTTCATGAAGTTCGTGTTAGGGAGGAGATTGGAGTGAAGTTGATGGTGACAAGGATTGATTTTTGGTTATATTTATTTGTGTATTTTTTCAGTGCAACACTTGGGTTGGTGTTCTTGAACAACTTGGGACAGATTGCAGAGTCCAGGGGCTCATCTAGGACTTCTTCTCTAGTCTCTTTATCCTCTTCATTTGGGTTTTTTGGCCGTCTCATGCCTTCTCTTTTGGACTATTTCTTCTCAAGGAGTAAGTACATGATTTCAAGACCAGCATTAATAGTTGCATTAATGGCACCAATAGCAGGAGTTTTCTTCTTGCTTCTCAACCCAGCCAACCTCTCCCTTTTCATCAGCACAGCAATTATAGGAGTGTGTTCTGGAGCAATTACTTCAATCGCAGTGTCCATAACCACAGAGCTGTTTGGGACCAAGAATTTCTCAGTGAACCACAATGTGGTGGTAGCCAACATATCAATCGGGTCTTTTGTGTTTGGGTACATGTCTGCTATTTTTTATCACAAGGATGGAAATCATGGTGGGGATGGGAAGTGCATGGGAATGGCTTGCTACAGAAGCACTTTTATTATATGGGGCTGCCTTTGTTTTCTGGGCACTGGCCTAGCTTTAATGCTCTACGCACGAACACGAAAGTTCTACGCCCAAAGATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

572

Amino Acids

63.0

Weight (kDa)

9.17

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 52 - 299 3.1e-77 Nodulin-like
NFD4_C PF23262 354 - 568 2.5e-102 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 857, 1336
AccB7I CCANNNNNTGG 1 cut(s) 1498
AccI GTMKAC 1 cut(s) 818
AccII CGCG 1 cut(s) 797
AciI CCGC 2 cut(s) 797, 828
AclWI GGATC 1 cut(s) 497
AcoI YGGCCR 1 cut(s) 1249
AcsI RAATTY 1 cut(s) 1477
AcuI CTGAAG 1 cut(s) 170
AcyI GRCGYC 1 cut(s) 396
AdeI CACNNNGTG 2 cut(s) 501, 1498
AfaI GTAC 4 cut(s) 24, 392, 1298, 1541
AfiI CCNNNNNNNGG 2 cut(s) 1346, 1498
AflIII ACRYGT 1 cut(s) 1542
AgsI TTSAA 3 cut(s) 1165, 1308, 1437
AjnI CCWGG 1 cut(s) 1190
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 7 cut(s) 221, 235, 268, 749, 999, 1462, 1667
AluI AGCT 7 cut(s) 221, 235, 268, 749, 999, 1462, 1667
Alw26I GTCTC 3 cut(s) 705, 1225, 1259
AlwI GGATC 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 4 cut(s) 189, 645, 1249, 1660
ApeKI GCWGC 2 cut(s) 329, 1638
ApoI RAATTY 1 cut(s) 1477
AseI ATTAAT 2 cut(s) 1319, 1331
AspLEI GCGC 2 cut(s) 584, 611
AspS9I GGNCC 1 cut(s) 1470
AsuHPI GGTGA 5 cut(s) 54, 618, 811, 898, 1105
AvaII GGWCC 1 cut(s) 1470
BaeGI GKGCMC 1 cut(s) 1658
BanI GGYRCC 2 cut(s) 857, 1336
BanII GRGCYC 1 cut(s) 1199
BbsI GAAGAC 2 cut(s) 117, 615
BbvI GCAGC 2 cut(s) 316, 1625
BccI CCATC 6 cut(s) 200, 341, 718, 1084, 1562, 1580
BceAI ACGGC 4 cut(s) 106, 409, 533, 1236
BciT130I CCWGG 1 cut(s) 1192
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 3 cut(s) 705, 1225, 1259
BfaI CTAG 6 cut(s) 167, 353, 1041, 1203, 1217, 1664
BfmI CTRYAG 2 cut(s) 263, 1612
BfoI RGCGCY 2 cut(s) 585, 612
BisI GCNGC 2 cut(s) 330, 1639
BlsI GCNGC 2 cut(s) 331, 1640
Bme1390I CCNGG 1 cut(s) 1192
Bme18I GGWCC 1 cut(s) 1470
BmgT120I GGNCC 1 cut(s) 1470
BmiI GGNNCC 4 cut(s) 202, 859, 1338, 1471
BmrFI CCNGG 1 cut(s) 1192
BpiI GAAGAC 2 cut(s) 117, 615
BpmI CTGGAG 1 cut(s) 1443
BpuEI CTTGAG 1 cut(s) 1270
BsaBI GATNNNNATC 1 cut(s) 1572
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 5 cut(s) 307, 549, 571, 642, 1191
BsaWI WCCGGW 2 cut(s) 785, 860
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 2 cut(s) 1346, 1498
Bse1I ACTGG 2 cut(s) 400, 1663
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 1572
BseBI CCWGG 1 cut(s) 1192
BseDI CCNNGG 5 cut(s) 307, 549, 571, 642, 1191
BseGI GGATG 2 cut(s) 1573, 1591
BseJI GATNNNNATC 1 cut(s) 1572
BseLI CCNNNNNNNGG 2 cut(s) 1346, 1498
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 2 cut(s) 303, 1497
BseNI ACTGG 2 cut(s) 400, 1663
BseRI GAGGAG 2 cut(s) 217, 1085
BseSI GKGCMC 1 cut(s) 1658
BseXI GCAGC 2 cut(s) 316, 1625
BseYI CCCAGC 5 cut(s) 217, 231, 470, 749, 1372
Bsh1236I CGCG 1 cut(s) 797
BshFI GGCC 4 cut(s) 191, 647, 1251, 1662
BshNI GGYRCC 2 cut(s) 857, 1336
BsiSI CCGG 3 cut(s) 321, 786, 861
BslFI GGGAC 3 cut(s) 241, 1188, 1483
BslI CCNNNNNNNGG 2 cut(s) 1346, 1498
BsmAI GTCTC 3 cut(s) 705, 1225, 1259
BsmBI CGTCTC 2 cut(s) 705, 1259
BsmFI GGGAC 3 cut(s) 241, 1188, 1483
BsnI GGCC 4 cut(s) 191, 647, 1251, 1662
Bsp1286I GDGCHC 2 cut(s) 1199, 1658
Bsp143I GATC 4 cut(s) 363, 489, 801, 1709
BspACI CCGC 2 cut(s) 797, 828
BspANI GGCC 4 cut(s) 191, 647, 1251, 1662
BspCNI CTCAG 2 cut(s) 302, 1496
BspFNI CGCG 1 cut(s) 797
BspHI TCATGA 2 cut(s) 360, 1051
BspLI GGNNCC 4 cut(s) 202, 859, 1338, 1471
BspPI GGATC 1 cut(s) 497
BspQI GCTCTTC 1 cut(s) 631
BspT107I GGYRCC 2 cut(s) 857, 1336
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 2 cut(s) 400, 1663
BssECI CCNNGG 5 cut(s) 307, 549, 571, 642, 1191
BssMI GATC 4 cut(s) 363, 489, 801, 1709
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 3 cut(s) 307, 571, 642
Bst2UI CCWGG 1 cut(s) 1192
Bst4CI ACNGT 3 cut(s) 43, 463, 499
Bst6I CTCTTC 5 cut(s) 413, 631, 946, 969, 1237
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 1609
BstDEI CTNAG 2 cut(s) 289, 1483
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 1573, 1591
BstFNI CGCG 1 cut(s) 797
BstH2I RGCGCY 2 cut(s) 585, 612
BstHHI GCGC 2 cut(s) 584, 611
BstKTI GATC 4 cut(s) 366, 492, 804, 1712
BstMAI GTCTC 3 cut(s) 705, 1225, 1259
BstMBI GATC 4 cut(s) 363, 489, 801, 1709
BstMWI GCNNNNNNNGC 4 cut(s) 293, 1334, 1343, 1617
BstNI CCWGG 1 cut(s) 1192
BstNSI RCATGY 1 cut(s) 1546
BstSCI CCNGG 1 cut(s) 1190
BstSFI CTRYAG 2 cut(s) 263, 1612
BstSLI GKGCMC 1 cut(s) 1658
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 2 cut(s) 316, 1625
BstV2I GAAGAC 2 cut(s) 117, 615
BsuRI GGCC 4 cut(s) 191, 647, 1251, 1662
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 1573, 1591
BtsI GCAGTG 1 cut(s) 1449
BtsIMutI CAGTG 5 cut(s) 495, 1144, 1449, 1491, 1656
Cac8I GCNNGC 1 cut(s) 1609
CaiI CAGNNNCTG 1 cut(s) 296
CciI TCATGA 2 cut(s) 360, 1051
CfoI GCGC 2 cut(s) 584, 611
Cfr13I GGNCC 1 cut(s) 1470
CseI GACGC 2 cut(s) 302, 385
Csp6I GTAC 4 cut(s) 23, 391, 1297, 1540
CviQI GTAC 4 cut(s) 23, 391, 1297, 1540
DdeI CTNAG 2 cut(s) 289, 1483
DpnI GATC 4 cut(s) 365, 491, 803, 1711
DpnII GATC 4 cut(s) 363, 489, 801, 1709
DraIII CACNNNGTG 2 cut(s) 501, 1498
EaeI YGGCCR 1 cut(s) 1249
Eam1104I CTCTTC 5 cut(s) 413, 631, 946, 969, 1237
EarI CTCTTC 5 cut(s) 413, 631, 946, 969, 1237
Eco130I CCWWGG 3 cut(s) 307, 571, 642
Eco147I AGGCCT 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 1199
Eco47I GGWCC 1 cut(s) 1470
Eco57I CTGAAG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 1190
EcoT14I CCWWGG 3 cut(s) 307, 571, 642
EcoT38I GRGCYC 1 cut(s) 1199
ErhI CCWWGG 3 cut(s) 307, 571, 642
Esp3I CGTCTC 2 cut(s) 705, 1259
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 3 cut(s) 241, 1188, 1483
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 1 cut(s) 179
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 1 cut(s) 818
Fnu4HI GCNGC 2 cut(s) 330, 1639
FokI GGATG 2 cut(s) 1580, 1598
FriOI GRGCYC 1 cut(s) 1199
Fsp4HI GCNGC 2 cut(s) 330, 1639
FspBI CTAG 6 cut(s) 167, 353, 1041, 1203, 1217, 1664
GlaI GCGC 2 cut(s) 583, 610
GluI GCNGC 2 cut(s) 330, 1639
GsaI CCCAGC 5 cut(s) 221, 235, 474, 753, 1376
GsuI CTGGAG 1 cut(s) 1443
HaeII RGCGCY 2 cut(s) 585, 612
HaeIII GGCC 4 cut(s) 191, 647, 1251, 1662
HapII CCGG 3 cut(s) 321, 786, 861
HgaI GACGC 2 cut(s) 302, 385
HhaI GCGC 2 cut(s) 584, 611
Hin1I GRCGYC 1 cut(s) 396
Hin6I GCGC 2 cut(s) 582, 609
HinP1I GCGC 2 cut(s) 582, 609
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 4 cut(s) 174, 789, 889, 1187
HpaII CCGG 3 cut(s) 321, 786, 861
HphI GGTGA 5 cut(s) 54, 618, 811, 898, 1105
Hpy166II GTNNAC 4 cut(s) 25, 819, 940, 1489
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 7 cut(s) 361, 710, 924, 1052, 1162, 1308, 1422
Hpy8I GTNNAC 4 cut(s) 25, 819, 940, 1489
HpyAV CCTTC 5 cut(s) 292, 444, 723, 1012, 1272
HpyCH4III ACNGT 3 cut(s) 43, 463, 499
HpyCH4IV ACGT 3 cut(s) 243, 714, 936
HpyCH4V TGCA 7 cut(s) 51, 57, 516, 1142, 1184, 1328, 1596
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 1334, 1343, 1617
HpyF3I CTNAG 2 cut(s) 289, 1483
HpySE526I ACGT 3 cut(s) 243, 714, 936
Hsp92I GRCGYC 1 cut(s) 396
HspAI GCGC 2 cut(s) 582, 609
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 4 cut(s) 363, 489, 801, 1709
LguI GCTCTTC 1 cut(s) 631
LmnI GCTCC 2 cut(s) 996, 1424
Lsp1109I GCAGC 2 cut(s) 316, 1625
MaeI CTAG 6 cut(s) 167, 353, 1041, 1203, 1217, 1664
MaeII ACGT 3 cut(s) 243, 714, 936
MaeIII GTNAC 2 cut(s) 566, 1093
MalI GATC 4 cut(s) 365, 491, 803, 1711
MboI GATC 4 cut(s) 363, 489, 801, 1709
MfeI CAATTG 2 cut(s) 777, 894
MhlI GDGCHC 2 cut(s) 1199, 1658
MluCI AATT 9 cut(s) 87, 543, 777, 847, 894, 961, 1404, 1428, 1477
MlyI GAGTC 1 cut(s) 1196
MseI TTAA 6 cut(s) 161, 851, 875, 1319, 1331, 1670
MslI CAYNNNNRTG 3 cut(s) 767, 1566, 1601
MspI CCGG 3 cut(s) 321, 786, 861
MspR9I CCNGG 1 cut(s) 1192
MunI CAATTG 2 cut(s) 777, 894
MvaI CCWGG 1 cut(s) 1192
MvnI CGCG 1 cut(s) 797
MwoI GCNNNNNNNGC 4 cut(s) 293, 1334, 1343, 1617
NdeI CATATG 1 cut(s) 179
NdeII GATC 4 cut(s) 363, 489, 801, 1709
NlaIV GGNNCC 4 cut(s) 202, 859, 1338, 1471
NmuCI GTSAC 1 cut(s) 1093
NspI RCATGY 1 cut(s) 1546
PagI TCATGA 2 cut(s) 360, 1051
PceI AGGCCT 1 cut(s) 647
PciI ACATGT 1 cut(s) 1542
PciSI GCTCTTC 1 cut(s) 631
PfeI GAWTC 3 cut(s) 174, 789, 889
PflMI CCANNNNNTGG 1 cut(s) 1498
PkrI GCNGC 2 cut(s) 331, 1640
PleI GAGTC 1 cut(s) 1195
PpsI GAGTC 1 cut(s) 1195
PscI ACATGT 1 cut(s) 1542
PshBI ATTAAT 2 cut(s) 1319, 1331
Psp6I CCWGG 1 cut(s) 1190
PspFI CCCAGC 5 cut(s) 217, 231, 470, 749, 1372
PspGI CCWGG 1 cut(s) 1190
PspN4I GGNNCC 4 cut(s) 202, 859, 1338, 1471
PspPI GGNCC 1 cut(s) 1470
PstNI CAGNNNCTG 1 cut(s) 296
RsaI GTAC 4 cut(s) 24, 392, 1298, 1541
RsaNI GTAC 4 cut(s) 23, 391, 1297, 1540
RseI CAYNNNNRTG 3 cut(s) 767, 1566, 1601
SalI GTCGAC 1 cut(s) 817
SapI GCTCTTC 1 cut(s) 631
SaqAI TTAA 6 cut(s) 161, 851, 875, 1319, 1331, 1670
SatI GCNGC 2 cut(s) 330, 1639
Sau3AI GATC 4 cut(s) 363, 489, 801, 1709
Sau96I GGNCC 1 cut(s) 1470
SchI GAGTC 1 cut(s) 1196
ScrFI CCNGG 1 cut(s) 1192
SduI GDGCHC 2 cut(s) 1199, 1658
SfcI CTRYAG 2 cut(s) 263, 1612
SinI GGWCC 1 cut(s) 1470
SmiMI CAYNNNNRTG 3 cut(s) 767, 1566, 1601
SmlI CTYRAG 1 cut(s) 1285
SmoI CTYRAG 1 cut(s) 1285
Sse9I AATT 9 cut(s) 87, 543, 777, 847, 894, 961, 1404, 1428, 1477
SseBI AGGCCT 1 cut(s) 647
SsiI CCGC 2 cut(s) 797, 828
SspMI CTAG 6 cut(s) 167, 353, 1041, 1203, 1217, 1664
StuI AGGCCT 1 cut(s) 647
StyD4I CCNGG 1 cut(s) 1190
StyI CCWWGG 3 cut(s) 307, 571, 642
TaaI ACNGT 3 cut(s) 43, 463, 499
TaiI ACGT 3 cut(s) 246, 717, 939
TaqI TCGA 2 cut(s) 709, 818
TaqII GACCGA 1 cut(s) 368
TasI AATT 9 cut(s) 87, 543, 777, 847, 894, 961, 1404, 1428, 1477
TatI WGTACW 1 cut(s) 1296
TfiI GAWTC 3 cut(s) 174, 789, 889
Tru1I TTAA 6 cut(s) 161, 851, 875, 1319, 1331, 1670
Tru9I TTAA 6 cut(s) 161, 851, 875, 1319, 1331, 1670
TscAI CASTG 5 cut(s) 502, 1144, 1449, 1491, 1663
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 2 cut(s) 329, 1638
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 7 cut(s) 81, 400, 751, 1040, 1068, 1224, 1381
TspRI CASTG 5 cut(s) 502, 1144, 1449, 1491, 1663
Van91I CCANNNNNTGG 1 cut(s) 1498
VpaK11BI GGWCC 1 cut(s) 1470
VspI ATTAAT 2 cut(s) 1319, 1331
XapI RAATTY 1 cut(s) 1477
XceI RCATGY 1 cut(s) 1546
XmiI GTMKAC 1 cut(s) 818
XspI CTAG 6 cut(s) 167, 353, 1041, 1203, 1217, 1664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.