Rh5BG492200

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001224
Physical Location & Seq
Forward (+)
78416005 .. 78417303
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG492200.1

Sequence Viewer

Length: 1299 bp
ATGTCTTCCTCATCAAATGCTTTCCAATGGTTAAGCCTAGTGGCCATCATATGGCTTCAGTCCATAAATGGAACAAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTATCCTTGTCCCAACTACAAATCAACAATCTCGTCTTTGCCTCCGACGCAGGGAAGCTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCGAGTCCTCATGATCGGCTCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAGACAAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCCGGAAATAGCGTCTGTTGGATCAACACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTAGGACTAACAACAAGTTATCAAGGATTGAGTGCAAAAATCTACACAGATATTGTTAGTACTGCTTTTTCTTCTTCACCTCAAAAAAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCGAGAAACATGGCAGCAGGGTTCAATTTGATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAATGATAGGTACCGGAGTGTTCCTACTGATCCCCTTGGTAATCCCGACTGTAGTAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAAAGTGGTGTAGTTAAACAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATGATGAGGTTGGTGAGGTACAGGTTAATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTGTATTTTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCGGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCCTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCAATTCTGGACTATTTCTTCTCAAGGCAACTACTCCCGTCCCCAGTTTTCAGTAATTTTTTTAAATTACATTTTGAAAAGTATATTGTCATATTAAAGTGGAATGTGCTTGGTAGAAATATTTTTGAAGCCAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

432

Amino Acids

48.14

Weight (kDa)

8.31

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 252 6.4e-72 Nodulin-like
NFD4_C PF23262 318 - 394 1.6e-35 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 568, 719
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 1 cut(s) 1079
AclWI GGATC 3 cut(s) 368, 697, 733
AcoI YGGCCR 3 cut(s) 42, 339, 1144
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 5 cut(s) 481, 721, 821, 904, 936
AfiI CCNNNNNNNGG 2 cut(s) 51, 168
AgsI TTSAA 5 cut(s) 631, 1060, 1161, 1235, 1286
AhlI ACTAGT 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 1 cut(s) 1154
AlwI GGATC 3 cut(s) 368, 697, 733
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 42, 339, 516, 847, 1144
ApeKI GCWGC 2 cut(s) 200, 620
Asp718I GGTACC 1 cut(s) 719
AsuHPI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 2 cut(s) 568, 719
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 2 cut(s) 187, 632
BccI CCATC 3 cut(s) 53, 786, 979
BceAI ACGGC 3 cut(s) 280, 656, 1131
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 1 cut(s) 1154
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 4 cut(s) 38, 551, 800, 1019
BfmI CTRYAG 2 cut(s) 420, 759
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 2 cut(s) 201, 621
BlsI GCNGC 2 cut(s) 202, 622
BmcAI AGTACT 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 248
BmiI GGNNCC 4 cut(s) 570, 606, 721, 797
BmrI ACTGGG 1 cut(s) 1196
BmuI ACTGGG 1 cut(s) 1196
BpmI CTGGAG 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 3 cut(s) 92, 872, 1165
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 333, 744, 1065
BsaWI WCCGGW 2 cut(s) 659, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 168
Bse1I ACTGG 2 cut(s) 681, 1202
Bse3DI GCAATG 2 cut(s) 195, 651
BseDI CCNNGG 3 cut(s) 333, 744, 1065
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 51, 168
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 681, 1202
BseRI GAGGAG 1 cut(s) 980
BseXI GCAGC 2 cut(s) 187, 632
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BshNI GGYRCC 2 cut(s) 568, 719
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 192, 342, 660, 723
BslFI GGGAC 3 cut(s) 112, 1083, 1183
BslI CCNNNNNNNGG 2 cut(s) 51, 168
BsmAI GTCTC 1 cut(s) 1154
BsmBI CGTCTC 1 cut(s) 1154
BsmFI GGGAC 3 cut(s) 112, 1083, 1183
BsnI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 6 cut(s) 234, 360, 689, 696, 738, 838
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 1 cut(s) 1079
BspANI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BspHI TCATGA 2 cut(s) 231, 916
BspLI GGNNCC 4 cut(s) 570, 606, 721, 797
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 3 cut(s) 368, 697, 733
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 2 cut(s) 568, 719
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1202
BssECI CCNNGG 3 cut(s) 333, 744, 1065
BssMI GATC 6 cut(s) 234, 360, 689, 696, 738, 838
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 3 cut(s) 333, 744, 1065
Bst4CI ACNGT 3 cut(s) 370, 760, 907
Bst6I CTCTTC 2 cut(s) 885, 1132
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstDEI CTNAG 2 cut(s) 703, 1098
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 6 cut(s) 237, 363, 692, 699, 741, 841
BstMAI GTCTC 1 cut(s) 1154
BstMBI GATC 6 cut(s) 234, 360, 689, 696, 738, 838
BstMWI GCNNNNNNNGC 2 cut(s) 164, 855
BstSFI CTRYAG 2 cut(s) 420, 759
BstV1I GCAGC 2 cut(s) 187, 632
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 5 cut(s) 44, 341, 518, 849, 1146
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 912, 1039
BveI ACCTGC 1 cut(s) 901
CciI TCATGA 2 cut(s) 231, 916
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 5 cut(s) 480, 720, 820, 903, 935
CviAII CATG 6 cut(s) 232, 334, 616, 917, 947, 1153
CviQI GTAC 5 cut(s) 480, 720, 820, 903, 935
DdeI CTNAG 2 cut(s) 703, 1098
DpnI GATC 6 cut(s) 236, 362, 691, 698, 740, 840
DpnII GATC 6 cut(s) 234, 360, 689, 696, 738, 838
DraI TTTAAA 1 cut(s) 1222
EaeI YGGCCR 3 cut(s) 42, 339, 1144
Eam1104I CTCTTC 2 cut(s) 885, 1132
EarI CTCTTC 2 cut(s) 885, 1132
Eco130I CCWWGG 3 cut(s) 333, 744, 1065
Eco147I AGGCCT 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 3 cut(s) 333, 744, 1065
ErhI CCWWGG 3 cut(s) 333, 744, 1065
Esp3I CGTCTC 1 cut(s) 1154
FaeI CATG 6 cut(s) 235, 337, 619, 920, 950, 1156
FaqI GGGAC 3 cut(s) 112, 1083, 1183
FatI CATG 6 cut(s) 231, 333, 615, 916, 946, 1152
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 2 cut(s) 201, 621
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 2 cut(s) 201, 621
FspBI CTAG 4 cut(s) 38, 551, 800, 1019
GluI GCNGC 2 cut(s) 201, 621
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 5 cut(s) 44, 341, 518, 849, 1146
HapII CCGG 4 cut(s) 192, 342, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 235, 337, 619, 920, 950, 1156
HinfI GANTC 3 cut(s) 225, 663, 1082
HpaII CCGG 4 cut(s) 192, 342, 660, 723
HphI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 3 cut(s) 163, 773, 843
Hpy188III TCNNGA 8 cut(s) 109, 232, 754, 786, 917, 1057, 1086, 1166
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 94, 221, 1141, 1167
HpyCH4III ACNGT 3 cut(s) 370, 760, 907
HpyCH4V TGCA 4 cut(s) 455, 910, 991, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 855
HpyF3I CTNAG 2 cut(s) 703, 1098
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 235, 337, 619, 920, 950, 1156
KpnI GGTACC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 6 cut(s) 234, 360, 689, 696, 738, 838
LmnI GCTCC 2 cut(s) 604, 795
Lsp1109I GCAGC 2 cut(s) 187, 632
MaeI CTAG 4 cut(s) 38, 551, 800, 1019
MaeIII GTNAC 1 cut(s) 283
MalI GATC 6 cut(s) 236, 362, 691, 698, 740, 840
MboI GATC 6 cut(s) 234, 360, 689, 696, 738, 838
MfeI CAATTG 1 cut(s) 1041
MhlI GDGCHC 1 cut(s) 514
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 7 cut(s) 272, 631, 1041, 1161, 1213, 1223, 1291
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 234, 1091
MmeI TCCRAC 2 cut(s) 186, 338
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 5 cut(s) 32, 876, 942, 1221, 1253
MslI CAYNNNNRTG 2 cut(s) 335, 590
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 4 cut(s) 192, 342, 660, 723
MunI CAATTG 1 cut(s) 1041
MwoI GCNNNNNNNGC 2 cut(s) 164, 855
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 6 cut(s) 234, 360, 689, 696, 738, 838
NlaIII CATG 6 cut(s) 235, 337, 619, 920, 950, 1156
NlaIV GGNNCC 4 cut(s) 570, 606, 721, 797
NmeAIII GCCGAG 1 cut(s) 633
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 2 cut(s) 231, 916
PceI AGGCCT 1 cut(s) 518
PfeI GAWTC 1 cut(s) 663
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 2 cut(s) 202, 622
PleI GAGTC 2 cut(s) 233, 1090
PpsI GAGTC 2 cut(s) 233, 1090
PspN4I GGNNCC 4 cut(s) 570, 606, 721, 797
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 5 cut(s) 481, 721, 821, 904, 936
RsaNI GTAC 5 cut(s) 480, 720, 820, 903, 935
RseI CAYNNNNRTG 2 cut(s) 335, 590
SaqAI TTAA 5 cut(s) 32, 876, 942, 1221, 1253
SatI GCNGC 2 cut(s) 201, 621
Sau3AI GATC 6 cut(s) 234, 360, 689, 696, 738, 838
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 2 cut(s) 234, 1091
SduI GDGCHC 1 cut(s) 514
SfcI CTRYAG 2 cut(s) 420, 759
SfuI TTCGAA 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 3 cut(s) 107, 851, 1180
SmoI CTYRAG 3 cut(s) 107, 851, 1180
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 7 cut(s) 272, 631, 1041, 1161, 1213, 1223, 1291
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 1 cut(s) 1079
SspI AATATT 2 cut(s) 321, 1279
SspMI CTAG 4 cut(s) 38, 551, 800, 1019
StuI AGGCCT 1 cut(s) 518
StyI CCWWGG 3 cut(s) 333, 744, 1065
TaaI ACNGT 3 cut(s) 370, 760, 907
TaqI TCGA 1 cut(s) 391
TasI AATT 7 cut(s) 272, 631, 1041, 1161, 1213, 1223, 1291
TatI WGTACW 3 cut(s) 479, 819, 902
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 5 cut(s) 32, 876, 942, 1221, 1253
Tru9I TTAA 5 cut(s) 32, 876, 942, 1221, 1253
TscAI CASTG 2 cut(s) 912, 1039
TseI GCWGC 2 cut(s) 200, 620
TspDTI ATGAA 1 cut(s) 905
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 2 cut(s) 912, 1039
Van91I CCANNNNNTGG 1 cut(s) 51
XspI CTAG 4 cut(s) 38, 551, 800, 1019
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.