Rw5G038690

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001224
Physical Location & Seq
Reverse (-)
68342526 .. 68344274
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038690.1

Sequence Viewer

Length: 1584 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGGCATCATATGGCTTCAATCCATAAATGGAACAAACACAACCTTCCCTGCTTACTCATCCCAACTCAAGAACAGTCTCTCCATGTCCCAAATACAAATCAACAATCTCGTCTTCGCCTCTGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATAATCGGATCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTCACAAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATCAATACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGCTTCTCCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTGCCTTTTCTTCTTCACCTCATAAGAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCGTTACTAGTCTGTGCTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCAAGAAACATGGCAACGGGGTTCAATTTAATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCAGCTTAGACAGAGTGATAGGCACCGGAGTATTCCTACTGATGCCCTTGGTAATCCCAATTGTGGAAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTGGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAGCATCGTACAGTGCAGGTTCACAATGAGGTTGGTGAAGTACAGGTTCATCATGGGATTGGTGTTAAAGAGGAGATTGGAGTGACGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTTTATTTTTTCAGTGCAACAATTGGGCTCGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCCAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGATTATTTCTTCGCAAGGCCTGCATTCCTAATGGCATTAGCGGCACCAATAGCAGGAGTTTTTTTCTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTTCAGTGTCCATAACCACAGAGCTATTCGGGACCAAAAAATTCTCAATAAATCACAATGTGGTGGTTGCGAATATCTCAATCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTCTTTATAGTAAGGAAGGAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTACAGAATCACCTTCATTATCTGGGGTAGTCTTTGTACGTTTGGGACTGTCCTAGCTTTAACGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

527

Amino Acids

57.69

Weight (kDa)

8.56

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 253 8.1e-71 Nodulin-like
NFD4_C PF23262 318 - 524 1.1e-96 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AccB1I GGYRCC 3 cut(s) 568, 719, 1210
AciI CCGC 3 cut(s) 401, 1208, 1275
AclWI GGATC 3 cut(s) 247, 368, 697
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 1 cut(s) 1352
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1372
AfaI GTAC 5 cut(s) 821, 904, 936, 1273, 1516
AfiI CCNNNNNNNGG 2 cut(s) 760, 1220
AgsI TTSAA 3 cut(s) 59, 631, 1060
AhlI ACTAGT 1 cut(s) 550
AjnI CCWGG 1 cut(s) 849
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 6 cut(s) 20, 702, 1250, 1336, 1461, 1535
AluI AGCT 6 cut(s) 20, 702, 1250, 1336, 1461, 1535
Alw21I GWGCWC 2 cut(s) 514, 1295
Alw26I GTCTC 3 cut(s) 122, 1154, 1261
Alw44I GTGCAC 1 cut(s) 1291
AlwI GGATC 3 cut(s) 247, 368, 697
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 516, 847, 1144, 1184
ApaLI GTGCAC 1 cut(s) 1291
ApeKI GCWGC 3 cut(s) 200, 482, 1461
ApoI RAATTY 1 cut(s) 1352
AspS9I GGNCC 1 cut(s) 1344
AsuHPI GGTGA 6 cut(s) 322, 489, 589, 670, 941, 1480
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 1344
BaeGI GKGCMC 1 cut(s) 1295
BanI GGYRCC 3 cut(s) 568, 719, 1210
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 2 cut(s) 514, 1295
BbvI GCAGC 3 cut(s) 187, 469, 1448
BccI CCATC 3 cut(s) 786, 979, 1448
BceAI ACGGC 4 cut(s) 280, 529, 656, 1131
BciT130I CCWGG 1 cut(s) 851
BclI TGATCA 2 cut(s) 696, 838
BcoDI GTCTC 3 cut(s) 122, 1154, 1261
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1532
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 4 cut(s) 201, 483, 1209, 1462
BlsI GCNGC 4 cut(s) 202, 484, 1210, 1463
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 1344
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 1344
BmiI GGNNCC 6 cut(s) 570, 606, 721, 797, 1212, 1345
BmrFI CCNGG 1 cut(s) 851
BmsI GCATC 3 cut(s) 54, 729, 907
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 3 cut(s) 288, 872, 1317
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 333, 442, 744, 1065
BsaWI WCCGGW 2 cut(s) 659, 722
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1220
Bse1I ACTGG 2 cut(s) 681, 1300
Bse3DI GCAATG 2 cut(s) 195, 651
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 4 cut(s) 333, 442, 744, 1065
BseGI GGATG 2 cut(s) 98, 1094
BseLI CCNNNNNNNGG 2 cut(s) 760, 1220
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 681, 1300
BseRI GAGGAG 2 cut(s) 908, 980
BseSI GKGCMC 1 cut(s) 1295
BseXI GCAGC 3 cut(s) 187, 469, 1448
BseYI CCCAGC 1 cut(s) 1246
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 4 cut(s) 518, 849, 1146, 1186
BshNI GGYRCC 3 cut(s) 568, 719, 1210
BsiHKAI GWGCWC 2 cut(s) 514, 1295
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 192, 660, 723
BslFI GGGAC 5 cut(s) 112, 438, 1083, 1357, 1537
BslI CCNNNNNNNGG 2 cut(s) 760, 1220
BsmAI GTCTC 3 cut(s) 122, 1154, 1261
BsmBI CGTCTC 2 cut(s) 1154, 1261
BsmFI GGGAC 5 cut(s) 112, 438, 1083, 1357, 1537
BsmI GAATGC 1 cut(s) 1190
BsnI GGCC 4 cut(s) 518, 849, 1146, 1186
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 514, 1050, 1295
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 3 cut(s) 401, 1208, 1275
BspANI GGCC 4 cut(s) 518, 849, 1146, 1186
BspLI GGNNCC 6 cut(s) 570, 606, 721, 797, 1212, 1345
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 3 cut(s) 247, 368, 697
BspQI GCTCTTC 1 cut(s) 1431
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 3 cut(s) 568, 719, 1210
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1300
BssECI CCNNGG 4 cut(s) 333, 442, 744, 1065
BssMI GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 1049
BssT1I CCWWGG 4 cut(s) 333, 442, 744, 1065
Bst2BI CACGAG 1 cut(s) 1049
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 4 cut(s) 116, 370, 907, 1528
Bst6I CTCTTC 2 cut(s) 885, 1431
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 343, 1188
BstDEI CTNAG 1 cut(s) 703
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 2 cut(s) 98, 1094
BstKTI GATC 6 cut(s) 242, 363, 692, 699, 841, 1580
BstMAI GTCTC 3 cut(s) 122, 1154, 1261
BstMBI GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
BstMWI GCNNNNNNNGC 7 cut(s) 42, 164, 566, 855, 1187, 1208, 1217
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 849
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 1295
BstV1I GCAGC 3 cut(s) 187, 469, 1448
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 518, 849, 1146, 1186
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 2 cut(s) 98, 1094
BtsIMutI CAGTG 5 cut(s) 484, 912, 1039, 1293, 1323
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 343, 1188
Cfr13I GGNCC 1 cut(s) 1344
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 5 cut(s) 820, 903, 935, 1272, 1515
CviAII CATG 5 cut(s) 124, 334, 616, 947, 1153
CviQI GTAC 5 cut(s) 820, 903, 935, 1272, 1515
DdeI CTNAG 1 cut(s) 703
DpnI GATC 6 cut(s) 241, 362, 691, 698, 840, 1579
DpnII GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
DraIII CACNNNGTG 1 cut(s) 1372
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 2 cut(s) 885, 1431
EarI CTCTTC 2 cut(s) 885, 1431
Eco130I CCWWGG 4 cut(s) 333, 442, 744, 1065
Eco147I AGGCCT 2 cut(s) 518, 1186
Eco24I GRGCYC 1 cut(s) 1050
Eco47I GGWCC 1 cut(s) 1344
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 4 cut(s) 333, 442, 744, 1065
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 4 cut(s) 333, 442, 744, 1065
Esp3I CGTCTC 2 cut(s) 1154, 1261
FaeI CATG 5 cut(s) 127, 337, 619, 950, 1156
FaqI GGGAC 5 cut(s) 112, 438, 1083, 1357, 1537
FatI CATG 5 cut(s) 123, 333, 615, 946, 1152
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 2 cut(s) 696, 838
Fnu4HI GCNGC 4 cut(s) 201, 483, 1209, 1462
FokI GGATG 2 cut(s) 85, 1081
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 4 cut(s) 201, 483, 1209, 1462
FspBI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1532
GluI GCNGC 4 cut(s) 201, 483, 1209, 1462
GsaI CCCAGC 1 cut(s) 1250
GsuI CTGGAG 3 cut(s) 288, 872, 1317
HaeIII GGCC 4 cut(s) 518, 849, 1146, 1186
HapII CCGG 3 cut(s) 192, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 127, 337, 619, 950, 1156
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 3 cut(s) 663, 1082, 1485
HpaII CCGG 3 cut(s) 192, 660, 723
HphI GGTGA 6 cut(s) 322, 489, 589, 670, 941, 1480
Hpy166II GTNNAC 3 cut(s) 820, 916, 1293
Hpy188I TCNGA 4 cut(s) 163, 239, 773, 843
Hpy188III TCNNGA 6 cut(s) 109, 786, 1057, 1086, 1166, 1342
Hpy8I GTNNAC 3 cut(s) 820, 916, 1293
HpyAV CCTTC 6 cut(s) 94, 221, 1141, 1167, 1436, 1501
HpyCH4III ACNGT 4 cut(s) 116, 370, 907, 1528
HpyCH4IV ACGT 3 cut(s) 980, 1254, 1517
HpyCH4V TGCA 7 cut(s) 455, 910, 991, 1037, 1190, 1293, 1550
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 164, 566, 855, 1187, 1208, 1217
HpyF3I CTNAG 1 cut(s) 703
HpySE526I ACGT 3 cut(s) 980, 1254, 1517
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 127, 337, 619, 950, 1156
Ksp22I TGATCA 2 cut(s) 696, 838
Kzo9I GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
LguI GCTCTTC 1 cut(s) 1431
LmnI GCTCC 4 cut(s) 604, 795, 895, 1298
Lsp1109I GCAGC 3 cut(s) 187, 469, 1448
LweI GCATC 3 cut(s) 54, 729, 907
MaeI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1532
MaeII ACGT 3 cut(s) 980, 1254, 1517
MaeIII GTNAC 5 cut(s) 283, 437, 546, 976, 1478
MalI GATC 6 cut(s) 241, 362, 691, 698, 840, 1579
MboI GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
MfeI CAATTG 2 cut(s) 756, 1041
MhlI GDGCHC 3 cut(s) 514, 1050, 1295
MluCI AATT 6 cut(s) 272, 631, 756, 1041, 1302, 1352
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 2 cut(s) 338, 433
MnlI CCTC 9 cut(s) 19, 169, 239, 510, 874, 886, 916, 958, 1082
MseI TTAA 4 cut(s) 32, 635, 960, 1538
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 3 cut(s) 192, 660, 723
MspR9I CCNGG 1 cut(s) 851
MunI CAATTG 2 cut(s) 756, 1041
Mva1269I GAATGC 1 cut(s) 1190
MvaI CCWGG 1 cut(s) 851
MwoI GCNNNNNNNGC 7 cut(s) 42, 164, 566, 855, 1187, 1208, 1217
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
NlaIII CATG 5 cut(s) 127, 337, 619, 950, 1156
NlaIV GGNNCC 6 cut(s) 570, 606, 721, 797, 1212, 1345
NmuCI GTSAC 2 cut(s) 283, 976
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PceI AGGCCT 2 cut(s) 518, 1186
PciSI GCTCTTC 1 cut(s) 1431
PctI GAATGC 1 cut(s) 1190
PfeI GAWTC 2 cut(s) 663, 1485
PkrI GCNGC 4 cut(s) 202, 484, 1210, 1463
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
Psp6I CCWGG 1 cut(s) 849
PspFI CCCAGC 1 cut(s) 1246
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 6 cut(s) 570, 606, 721, 797, 1212, 1345
PspPI GGNCC 1 cut(s) 1344
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 5 cut(s) 821, 904, 936, 1273, 1516
RsaNI GTAC 5 cut(s) 820, 903, 935, 1272, 1515
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1431
SaqAI TTAA 4 cut(s) 32, 635, 960, 1538
SatI GCNGC 4 cut(s) 201, 483, 1209, 1462
Sau3AI GATC 6 cut(s) 239, 360, 689, 696, 838, 1577
Sau96I GGNCC 1 cut(s) 1344
SchI GAGTC 1 cut(s) 1091
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 3 cut(s) 514, 1050, 1295
SfaNI GCATC 3 cut(s) 54, 729, 907
SfcI CTRYAG 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 1 cut(s) 1344
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 6 cut(s) 272, 631, 756, 1041, 1302, 1352
SseBI AGGCCT 2 cut(s) 518, 1186
SsiI CCGC 3 cut(s) 401, 1208, 1275
SspI AATATT 1 cut(s) 321
SspMI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1532
StuI AGGCCT 2 cut(s) 518, 1186
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 4 cut(s) 333, 442, 744, 1065
TaaI ACNGT 4 cut(s) 116, 370, 907, 1528
TaiI ACGT 3 cut(s) 983, 1257, 1520
TaqI TCGA 1 cut(s) 391
TasI AATT 6 cut(s) 272, 631, 756, 1041, 1302, 1352
TatI WGTACW 2 cut(s) 819, 934
TauI GCSGC 1 cut(s) 1211
TfiI GAWTC 2 cut(s) 663, 1485
Tru1I TTAA 4 cut(s) 32, 635, 960, 1538
Tru9I TTAA 4 cut(s) 32, 635, 960, 1538
TscAI CASTG 5 cut(s) 484, 912, 1039, 1300, 1323
TseFI GTSAC 2 cut(s) 283, 976
TseI GCWGC 3 cut(s) 200, 482, 1461
Tsp45I GTSAC 2 cut(s) 283, 976
TspDTI ATGAA 3 cut(s) 932, 1464, 1483
TspRI CASTG 5 cut(s) 484, 912, 1039, 1300, 1323
VneI GTGCAC 1 cut(s) 1291
VpaK11BI GGWCC 1 cut(s) 1344
XapI RAATTY 1 cut(s) 1352
XspI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.