RLG00000013856

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001224
Physical Location & Seq
Forward (+)
43052595 .. 43054391
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013856

Sequence Viewer

Length: 1626 bp
ATGTCTTCATCCTCATCAAAAGCTTTCCAATGGTTAAGCCTTGTGGGCATCATATGGCTTCAATCCATAAACGGAACGAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTTCCTCTCCCAACTACAAATCAACAATCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTCTGGCTAGTCCTCATTATCGGCTCACTTCTCGGGTTGATTGGCTACGGTGTCCAATTTCTATTCGTAACCAACCAAATCTCATCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCCGGAAATAGTGTCTGTTGGATCAACACCGTTTGCTATGTTGTTTGCATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAGGGATTGAGTGCAAAAATCTACACAGACATCGTTGGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCCAAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTCTGTTGTAGTAGCACCATTTGTGAGACACATTGATGTGGGAACAGGGGAGCCCAGAAATATGGCAGCGGGGTTCAAATTAATGTTTGTGGTAACAATTGCCACCGGAATCTATGCCGTGATCACCAGTTTAGGATCAATGTCGTCAATCCACTTATCGCCATTGCAGGGTGTGATAGGTACCGTATTGTTCCTACTGATCCCCTTGGTCATCCCAATTGTGGAAAAAATGGGAGAAAAATTATCAAGGAGGTGGAGCCTAGACAGAGAGAACAGAGTTCATGACTTCAGCATAGAAGATCAGAAAGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAATATAGTACAGTGCAGGTTCACAATGAGGTTGGTGAAGTTCAGGTTCATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGAAAAGGATAGATTTCTGGTTGTATTTCCTAGTTTATATTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCATTTGGGTTCTTTGGCCGTCTCATGACTTCACTTCTGGACTATGTCTTCTCAAGAAGCAATTACATGATTTCAAGGCCTGCATTCCTAGTGACATTAGCGGCACCAATAGCAGGAGTTTTCTTCTTGCTTCTCAACCCAGCTAACGTCTCCCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCCTAACCACAGAGCTATTCGGGACCAAGAATTTCTCAATAAATCACAATGTGGTGGTTGTGAATATCTCAATCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTATTTATCGCAAGGAAGAAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTATAGGAGCACCTTCATTATTTGGGGTAGTCTTTGTACGTTAGGGACTACTCTAGCTTTAGTGCTATATGCAAGAACAAGGAAGTTTTACTCCAAAATATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

542

Amino Acids

59.29

Weight (kDa)

8.69

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 10 - 260 6.5e-73 Nodulin-like
NFD4_C PF23262 325 - 538 5e-99 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 922
Acc65I GGTACC 1 cut(s) 740
AccB1I GGYRCC 2 cut(s) 740, 1252
AciI CCGC 3 cut(s) 629, 1250, 1317
AclWI GGATC 3 cut(s) 374, 703, 754
AcoI YGGCCR 2 cut(s) 345, 1165
AcsI RAATTY 1 cut(s) 1393
AcuI CTGAAG 1 cut(s) 832
AdeI CACNNNGTG 1 cut(s) 1414
AfaI GTAC 4 cut(s) 742, 925, 1315, 1558
AfiI CCNNNNNNNGG 4 cut(s) 171, 728, 781, 1262
AgsI TTSAA 4 cut(s) 62, 637, 1081, 1224
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 447
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 5 cut(s) 23, 1292, 1378, 1503, 1577
AluI AGCT 5 cut(s) 23, 1292, 1378, 1503, 1577
Alw21I GWGCWC 2 cut(s) 1337, 1532
Alw26I GTCTC 4 cut(s) 565, 580, 1175, 1303
Alw44I GTGCAC 1 cut(s) 1333
AlwI GGATC 3 cut(s) 374, 703, 754
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 345, 522, 868, 1165, 1226
ApaLI GTGCAC 1 cut(s) 1333
ApeKI GCWGC 4 cut(s) 203, 488, 626, 1503
ApoI RAATTY 1 cut(s) 1393
AseI ATTAAT 1 cut(s) 641
Asp718I GGTACC 1 cut(s) 740
AspS9I GGNCC 1 cut(s) 1386
AsuHPI GGTGA 6 cut(s) 328, 404, 495, 676, 962, 1021
AsuII TTCGAA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1386
BaeGI GKGCMC 1 cut(s) 1337
BanI GGYRCC 2 cut(s) 740, 1252
BanII GRGCYC 1 cut(s) 615
BbsI GAAGAC 2 cut(s) 148, 1189
Bbv12I GWGCWC 2 cut(s) 1337, 1532
BbvI GCAGC 4 cut(s) 190, 475, 638, 1490
BccI CCATC 2 cut(s) 1000, 1490
BceAI ACGGC 2 cut(s) 662, 1152
BciT130I CCWGG 1 cut(s) 449
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 4 cut(s) 565, 580, 1175, 1303
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 6 cut(s) 227, 557, 821, 1040, 1238, 1574
BfuAI ACCTGC 1 cut(s) 922
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 5 cut(s) 204, 489, 627, 1251, 1504
BlsI GCNGC 5 cut(s) 205, 490, 628, 1252, 1505
Bme1390I CCNGG 1 cut(s) 449
Bme18I GGWCC 1 cut(s) 1386
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1386
BmiI GGNNCC 5 cut(s) 612, 742, 818, 1254, 1387
BmrFI CCNGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 2 cut(s) 148, 1189
BpmI CTGGAG 2 cut(s) 294, 1359
Bpu14I TTCGAA 1 cut(s) 397
BpuEI CTTGAG 3 cut(s) 95, 893, 1186
BsaJI CCNNGG 5 cut(s) 339, 448, 519, 765, 1086
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 728, 781, 1262
Bse1I ACTGG 2 cut(s) 687, 1342
Bse3DI GCAATG 2 cut(s) 198, 722
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 339, 448, 519, 765, 1086
BseGI GGATG 3 cut(s) 8, 101, 771
BseLI CCNNNNNNNGG 4 cut(s) 171, 728, 781, 1262
BseMI GCAATG 2 cut(s) 198, 722
BseNI ACTGG 2 cut(s) 687, 1342
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 1337
BseXI GCAGC 4 cut(s) 190, 475, 638, 1490
BseYI CCCAGC 1 cut(s) 1288
BsgI GTGCAG 1 cut(s) 950
BshFI GGCC 5 cut(s) 347, 524, 870, 1167, 1228
BshNI GGYRCC 2 cut(s) 740, 1252
BsiHKAI GWGCWC 2 cut(s) 1337, 1532
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 195, 348, 666
BslFI GGGAC 5 cut(s) 444, 1104, 1348, 1399, 1579
BslI CCNNNNNNNGG 4 cut(s) 171, 728, 781, 1262
BsmAI GTCTC 4 cut(s) 565, 580, 1175, 1303
BsmBI CGTCTC 2 cut(s) 1175, 1303
BsmFI GGGAC 5 cut(s) 444, 1104, 1348, 1399, 1579
BsmI GAATGC 2 cut(s) 393, 1232
BsnI GGCC 5 cut(s) 347, 524, 870, 1167, 1228
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 3 cut(s) 615, 1337, 1532
Bsp143I GATC 5 cut(s) 366, 681, 695, 759, 859
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 629, 1250, 1317
BspANI GGCC 5 cut(s) 347, 524, 870, 1167, 1228
BspHI TCATGA 2 cut(s) 841, 1173
BspLI GGNNCC 5 cut(s) 612, 742, 818, 1254, 1387
BspMI ACCTGC 1 cut(s) 922
BspPI GGATC 3 cut(s) 374, 703, 754
BspT104I TTCGAA 1 cut(s) 397
BspT107I GGYRCC 2 cut(s) 740, 1252
BsrDI GCAATG 2 cut(s) 198, 722
BsrI ACTGG 2 cut(s) 687, 1342
BssECI CCNNGG 5 cut(s) 339, 448, 519, 765, 1086
BssMI GATC 5 cut(s) 366, 681, 695, 759, 859
BssT1I CCWWGG 4 cut(s) 339, 519, 765, 1086
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 4 cut(s) 269, 376, 745, 928
Bst6I CTCTTC 2 cut(s) 128, 906
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 1230
BstDEI CTNAG 1 cut(s) 1119
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 8, 101, 771
BstKTI GATC 5 cut(s) 369, 684, 698, 762, 862
BstMAI GTCTC 4 cut(s) 565, 580, 1175, 1303
BstMBI GATC 5 cut(s) 366, 681, 695, 759, 859
BstMWI GCNNNNNNNGC 4 cut(s) 45, 167, 876, 1259
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 447
BstSLI GKGCMC 1 cut(s) 1337
BstV1I GCAGC 4 cut(s) 190, 475, 638, 1490
BstV2I GAAGAC 2 cut(s) 148, 1189
BstXI CCANNNNNNTGG 2 cut(s) 318, 622
BsuRI GGCC 5 cut(s) 347, 524, 870, 1167, 1228
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 8, 101, 771
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 4 cut(s) 933, 1060, 1335, 1365
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 1 cut(s) 1230
CciI TCATGA 2 cut(s) 841, 1173
Cfr13I GGNCC 1 cut(s) 1386
CseI GACGC 1 cut(s) 176
Csp6I GTAC 4 cut(s) 741, 924, 1314, 1557
CviAII CATG 5 cut(s) 340, 842, 968, 1174, 1216
CviQI GTAC 4 cut(s) 741, 924, 1314, 1557
DdeI CTNAG 1 cut(s) 1119
DpnI GATC 5 cut(s) 368, 683, 697, 761, 861
DpnII GATC 5 cut(s) 366, 681, 695, 759, 859
DraIII CACNNNGTG 1 cut(s) 1414
EaeI YGGCCR 2 cut(s) 345, 1165
Eam1104I CTCTTC 2 cut(s) 128, 906
EarI CTCTTC 2 cut(s) 128, 906
Eco130I CCWWGG 4 cut(s) 339, 519, 765, 1086
Eco147I AGGCCT 3 cut(s) 524, 870, 1228
Eco24I GRGCYC 1 cut(s) 615
Eco47I GGWCC 1 cut(s) 1386
Eco57I CTGAAG 1 cut(s) 832
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 4 cut(s) 339, 519, 765, 1086
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 4 cut(s) 339, 519, 765, 1086
Esp3I CGTCTC 2 cut(s) 1175, 1303
FaeI CATG 5 cut(s) 343, 845, 971, 1177, 1219
FaqI GGGAC 5 cut(s) 444, 1104, 1348, 1399, 1579
FatI CATG 5 cut(s) 339, 841, 967, 1173, 1215
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 1 cut(s) 53
FbaI TGATCA 1 cut(s) 681
Fnu4HI GCNGC 5 cut(s) 204, 489, 627, 1251, 1504
FokI GGATG 2 cut(s) 88, 758
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 5 cut(s) 204, 489, 627, 1251, 1504
FspBI CTAG 6 cut(s) 227, 557, 821, 1040, 1238, 1574
GluI GCNGC 5 cut(s) 204, 489, 627, 1251, 1504
GsaI CCCAGC 1 cut(s) 1292
GsuI CTGGAG 2 cut(s) 294, 1359
HaeIII GGCC 5 cut(s) 347, 524, 870, 1167, 1228
HapII CCGG 3 cut(s) 195, 348, 666
HgaI GACGC 1 cut(s) 176
Hin1II CATG 5 cut(s) 343, 845, 971, 1177, 1219
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 2 cut(s) 669, 1103
HpaII CCGG 3 cut(s) 195, 348, 666
HphI GGTGA 6 cut(s) 328, 404, 495, 676, 962, 1021
Hpy166II GTNNAC 2 cut(s) 937, 1335
Hpy188I TCNGA 2 cut(s) 166, 864
Hpy188III TCNNGA 8 cut(s) 112, 842, 1078, 1107, 1174, 1187, 1203, 1384
Hpy8I GTNNAC 2 cut(s) 937, 1335
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 3 cut(s) 97, 224, 1543
HpyCH4III ACNGT 4 cut(s) 269, 376, 745, 928
HpyCH4IV ACGT 2 cut(s) 1296, 1559
HpyCH4V TGCA 9 cut(s) 393, 461, 727, 931, 1058, 1232, 1335, 1358, 1592
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 167, 876, 1259
HpyF3I CTNAG 1 cut(s) 1119
HpySE526I ACGT 2 cut(s) 1296, 1559
Hsp92II CATG 5 cut(s) 343, 845, 971, 1177, 1219
KpnI GGTACC 1 cut(s) 744
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 5 cut(s) 366, 681, 695, 759, 859
LmnI GCTCC 4 cut(s) 610, 816, 1340, 1527
Lsp1109I GCAGC 4 cut(s) 190, 475, 638, 1490
LweI GCATC 1 cut(s) 57
MaeI CTAG 6 cut(s) 227, 557, 821, 1040, 1238, 1574
MaeII ACGT 2 cut(s) 1296, 1559
MaeIII GTNAC 4 cut(s) 286, 443, 652, 1240
MalI GATC 5 cut(s) 368, 683, 697, 761, 861
MboI GATC 5 cut(s) 366, 681, 695, 759, 859
MfeI CAATTG 3 cut(s) 657, 777, 1062
MhlI GDGCHC 3 cut(s) 615, 1337, 1532
MluCI AATT 9 cut(s) 275, 638, 657, 777, 800, 1062, 1210, 1344, 1393
MlyI GAGTC 1 cut(s) 1112
MmeI TCCRAC 2 cut(s) 189, 344
MseI TTAA 2 cut(s) 35, 641
MslI CAYNNNNRTG 3 cut(s) 341, 594, 596
MspA1I CMGCKG 1 cut(s) 629
MspI CCGG 3 cut(s) 195, 348, 666
MspR9I CCNGG 1 cut(s) 449
MunI CAATTG 3 cut(s) 657, 777, 1062
Mva1269I GAATGC 2 cut(s) 393, 1232
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 4 cut(s) 45, 167, 876, 1259
NcoI CCATGG 1 cut(s) 339
NdeI CATATG 1 cut(s) 53
NdeII GATC 5 cut(s) 366, 681, 695, 759, 859
NlaIII CATG 5 cut(s) 343, 845, 971, 1177, 1219
NlaIV GGNNCC 5 cut(s) 612, 742, 818, 1254, 1387
NmuCI GTSAC 1 cut(s) 1240
NspV TTCGAA 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 341
PagI TCATGA 2 cut(s) 841, 1173
PceI AGGCCT 3 cut(s) 524, 870, 1228
PctI GAATGC 2 cut(s) 393, 1232
PfeI GAWTC 1 cut(s) 669
PflFI GACNNNGTC 1 cut(s) 1193
PkrI GCNGC 5 cut(s) 205, 490, 628, 1252, 1505
PleI GAGTC 1 cut(s) 1111
PpsI GAGTC 1 cut(s) 1111
PshBI ATTAAT 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 1288
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 612, 742, 818, 1254, 1387
PspPI GGNCC 1 cut(s) 1386
PsyI GACNNNGTC 1 cut(s) 1193
RsaI GTAC 4 cut(s) 742, 925, 1315, 1558
RsaNI GTAC 4 cut(s) 741, 924, 1314, 1557
RseI CAYNNNNRTG 3 cut(s) 341, 594, 596
SaqAI TTAA 2 cut(s) 35, 641
SatI GCNGC 5 cut(s) 204, 489, 627, 1251, 1504
Sau3AI GATC 5 cut(s) 366, 681, 695, 759, 859
Sau96I GGNCC 1 cut(s) 1386
SchI GAGTC 1 cut(s) 1112
ScrFI CCNGG 1 cut(s) 449
SduI GDGCHC 3 cut(s) 615, 1337, 1532
SfaNI GCATC 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 1386
SmiMI CAYNNNNRTG 3 cut(s) 341, 594, 596
SmlI CTYRAG 3 cut(s) 110, 872, 1201
SmoI CTYRAG 3 cut(s) 110, 872, 1201
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 9 cut(s) 275, 638, 657, 777, 800, 1062, 1210, 1344, 1393
SseBI AGGCCT 3 cut(s) 524, 870, 1228
SsiI CCGC 3 cut(s) 629, 1250, 1317
SspI AATATT 1 cut(s) 327
SspMI CTAG 6 cut(s) 227, 557, 821, 1040, 1238, 1574
StuI AGGCCT 3 cut(s) 524, 870, 1228
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 4 cut(s) 339, 519, 765, 1086
TaaI ACNGT 4 cut(s) 269, 376, 745, 928
TaiI ACGT 2 cut(s) 1299, 1562
TaqI TCGA 1 cut(s) 397
TasI AATT 9 cut(s) 275, 638, 657, 777, 800, 1062, 1210, 1344, 1393
TatI WGTACW 1 cut(s) 923
TauI GCSGC 1 cut(s) 1253
TfiI GAWTC 1 cut(s) 669
Tru1I TTAA 2 cut(s) 35, 641
Tru9I TTAA 2 cut(s) 35, 641
TscAI CASTG 4 cut(s) 933, 1060, 1342, 1365
TseFI GTSAC 1 cut(s) 1240
TseI GCWGC 4 cut(s) 203, 488, 626, 1503
Tsp45I GTSAC 1 cut(s) 1240
TspDTI ATGAA 5 cut(s) 830, 953, 1140, 1506, 1525
TspGWI ACGGA 2 cut(s) 87, 396
TspRI CASTG 4 cut(s) 933, 1060, 1342, 1365
Tth111I GACNNNGTC 1 cut(s) 1193
VneI GTGCAC 1 cut(s) 1333
VpaK11BI GGWCC 1 cut(s) 1386
VspI ATTAAT 1 cut(s) 641
XapI RAATTY 1 cut(s) 1393
XspI CTAG 6 cut(s) 227, 557, 821, 1040, 1238, 1574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.