FvH4_2g11110

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001224
Physical Location & Seq
Reverse (-)
9687826 .. 9689902
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11110.t1

Sequence Viewer

Length: 1590 bp
ATGTCTTCTTCATCAAAAGCTTTCCAATGGTTAAGCCTTGTGGCCATCATATGGCTTCAATCCATAAACGGAACAAACATCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTCCATGTCCCAACTACAAATCAACAACCTCGTCTTCGCCTCTGACGCAGGCAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGTTAGTCCTCATCATTGGCTCGCTTCTCGGCTTGATTGGCTACGGTGTCCAATTTCTATTCGTAACCAACCAAATCTCATCTCCGGCATATTGGCAAATATTCTTGCTCACCATGGTGGCCGGAAATAGTGTCTGTTGGATCAACACCGTTTGCTATGTCGTTTGTATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTAGGACTAACCACAAGTTACCAAGGATTGAGTGCAAAAATCTACACTGATATTGTTGGTGCTGCTTTTTCTTCTGCACCTTATAATACAGCCAAGGCCTATCTTCTTCTAATCTCTCTCCTGCCTTTACTAGTCTGTATTGTAGTGGCACCATTTCTGAGAGACATTGATGTGGGAAGAGGGGAGCCCGGAAATATGGCAGGAGGGTTCAAATTAATGTTTGTGATAACCATTGCCACGGGAATCTATGCCGTGATCACCAGTTTAGGGTCAATGTCTATCGGCTTATCGCCATTGCATAATGTGATAGGTACCGCATTGTTCCTACTGATCCCCTTGGTAATCCCAACTTTGGAAAAAATTGGAGAAAAAGTATCAGGAAGGTGGAGCCTAGACAAAAATACTAGAGTGCACAATTTGAGCATAGATGATCAGAAAGGCCTTGAGAGCATAGAGAGTGGCGTAGTGAAAGAGGAAGACGAAGAGGAACATACTACAGTGCAGGTTAATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTAAAAAGGATAGATTTCTGGTTATATTTCCTAGTGTACTTTCTCAGTGCAACAATTGGGCTTCTTTTCTTGAACATCTTGGGACAGATAGCAGGGTCAAGAGGCTCATATAGGACTTCTTCTCTGGTTTCTTTATCTTCTTCATTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGACTACTTCTTCTCAAGAAGCAAGTATATGATTTCACGGCCTGCATTCCTGGTGGCATTAGCAGCACCAATAGCAGGAGCTTTCTTCTTGCTGCTCAACCAAGCTAACATCTCCCTTATCATGAGTACCGCCATTATAGGAGTGTGCACCGGAGCAATTACTTGTATTGCAGTGTCCATAACTACAGAGCTATTTGGGACAAAAAATTTCTCAATAAATCACAATGTGGTGGTTGCAAATATCTCAATCGGCTCGTTTGTTTTTGGCTACTCATCTGCTCTTCTTTATCGCAAGGAAGAAAATGAAGATGGTAGCTGCTTGGGAACGGCTTGTTACAGAAACACCTTCATTATCTGGGGTAGTCTTTGTTCGTTGGGGACTGTTCTAGCCTTGATGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

530

Amino Acids

57.72

Weight (kDa)

8.83

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 256 5.2e-74 Nodulin-like
NFD4_C PF23262 313 - 526 1.2e-99 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
Acc36I ACCTGC 1 cut(s) 913
Acc65I GGTACC 1 cut(s) 731
AccB1I GGYRCC 2 cut(s) 568, 731
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 2 cut(s) 735, 1281
AclWI GGATC 2 cut(s) 368, 745
AcoI YGGCCR 3 cut(s) 42, 339, 1129
AcsI RAATTY 2 cut(s) 1357, 1582
AdeI CACNNNGTG 1 cut(s) 1378
AfaI GTAC 3 cut(s) 733, 1010, 1279
AfiI CCNNNNNNNGG 4 cut(s) 51, 687, 772, 1226
AgsI TTSAA 3 cut(s) 59, 631, 1045
AhlI ACTAGT 1 cut(s) 550
AjnI CCWGG 1 cut(s) 1200
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 5 cut(s) 20, 1232, 1256, 1342, 1467
AluI AGCT 5 cut(s) 20, 1232, 1256, 1342, 1467
Alw21I GWGCWC 2 cut(s) 834, 1301
Alw26I GTCTC 2 cut(s) 576, 1139
Alw44I GTGCAC 2 cut(s) 830, 1297
AlwI GGATC 2 cut(s) 368, 745
AoxI GGCC 6 cut(s) 42, 339, 516, 859, 1129, 1190
ApaLI GTGCAC 2 cut(s) 830, 1297
ApeKI GCWGC 5 cut(s) 200, 482, 1214, 1243, 1467
ApoI RAATTY 2 cut(s) 1357, 1582
AseI ATTAAT 1 cut(s) 635
Asp718I GGTACC 1 cut(s) 731
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 4 cut(s) 73, 322, 398, 670
AsuII TTCGAA 1 cut(s) 391
BaeGI GKGCMC 2 cut(s) 834, 1301
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 2 cut(s) 568, 731
BanII GRGCYC 1 cut(s) 609
BbsI GAAGAC 2 cut(s) 145, 903
Bbv12I GWGCWC 2 cut(s) 834, 1301
BbvI GCAGC 5 cut(s) 187, 469, 1226, 1230, 1454
BccI CCATC 3 cut(s) 53, 964, 1454
BceAI ACGGC 4 cut(s) 656, 1116, 1205, 1494
BciT130I CCWGG 1 cut(s) 1202
BclI TGATCA 2 cut(s) 675, 850
BcnI CCSGG 1 cut(s) 609
BcoDI GTCTC 2 cut(s) 576, 1139
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 5 cut(s) 551, 812, 825, 1004, 1538
BfmI CTRYAG 3 cut(s) 420, 915, 1335
BfuAI ACCTGC 1 cut(s) 913
BisI GCNGC 5 cut(s) 201, 483, 1215, 1244, 1468
BlsI GCNGC 5 cut(s) 202, 484, 1216, 1245, 1469
Bme1390I CCNGG 2 cut(s) 609, 1202
BmiI GGNNCC 4 cut(s) 570, 606, 733, 809
BmrFI CCNGG 2 cut(s) 609, 1202
BmsI GCATC 1 cut(s) 1536
BpiI GAAGAC 2 cut(s) 145, 903
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 3 cut(s) 92, 884, 1150
BpuMI CCSGG 1 cut(s) 609
BsaJI CCNNGG 5 cut(s) 333, 442, 513, 657, 756
BsaWI WCCGGW 1 cut(s) 1301
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 777, 807
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 687, 772, 1226
Bse1I ACTGG 1 cut(s) 681
Bse3DI GCAATG 3 cut(s) 195, 651, 713
BseBI CCWGG 1 cut(s) 1202
BseDI CCNNGG 5 cut(s) 333, 442, 513, 657, 756
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 4 cut(s) 51, 687, 772, 1226
BseMI GCAATG 3 cut(s) 195, 651, 713
BseMII CTCAG 2 cut(s) 569, 1030
BseNI ACTGG 1 cut(s) 681
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 2 cut(s) 834, 1301
BseXI GCAGC 5 cut(s) 187, 469, 1226, 1230, 1454
BsgI GTGCAG 2 cut(s) 480, 941
BshFI GGCC 6 cut(s) 44, 341, 518, 861, 1131, 1192
BshNI GGYRCC 2 cut(s) 568, 731
BsiHKAI GWGCWC 2 cut(s) 834, 1301
BsiSI CCGG 5 cut(s) 192, 305, 342, 609, 1302
BslFI GGGAC 4 cut(s) 112, 1068, 1363, 1543
BslI CCNNNNNNNGG 4 cut(s) 51, 687, 772, 1226
BsmAI GTCTC 2 cut(s) 576, 1139
BsmBI CGTCTC 1 cut(s) 1139
BsmFI GGGAC 4 cut(s) 112, 1068, 1363, 1543
BsmI GAATGC 1 cut(s) 1196
BsnI GGCC 6 cut(s) 44, 341, 518, 861, 1131, 1192
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 609, 834, 1301
Bsp143I GATC 4 cut(s) 360, 675, 750, 850
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 2 cut(s) 735, 1281
BspANI GGCC 6 cut(s) 44, 341, 518, 861, 1131, 1192
BspCNI CTCAG 2 cut(s) 570, 1029
BspHI TCATGA 1 cut(s) 1272
BspLI GGNNCC 4 cut(s) 570, 606, 733, 809
BspMI ACCTGC 1 cut(s) 913
BspPI GGATC 2 cut(s) 368, 745
BspQI GCTCTTC 1 cut(s) 1437
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 2 cut(s) 568, 731
BsrDI GCAATG 3 cut(s) 195, 651, 713
BsrI ACTGG 1 cut(s) 681
BssECI CCNNGG 5 cut(s) 333, 442, 513, 657, 756
BssMI GATC 4 cut(s) 360, 675, 750, 850
BssT1I CCWWGG 4 cut(s) 333, 442, 513, 756
Bst2UI CCWGG 1 cut(s) 1202
Bst4CI ACNGT 4 cut(s) 266, 370, 919, 1534
Bst6I CTCTTC 3 cut(s) 592, 897, 1437
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 169, 242, 1194
BstDEI CTNAG 2 cut(s) 578, 1016
BstDSI CCRYGG 2 cut(s) 333, 657
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 4 cut(s) 363, 678, 753, 853
BstMAI GTCTC 2 cut(s) 576, 1139
BstMBI GATC 4 cut(s) 360, 675, 750, 850
BstMWI GCNNNNNNNGC 5 cut(s) 164, 258, 867, 1214, 1223
BstNI CCWGG 1 cut(s) 1202
BstSCI CCNGG 2 cut(s) 607, 1200
BstSFI CTRYAG 3 cut(s) 420, 915, 1335
BstSLI GKGCMC 2 cut(s) 834, 1301
BstV1I GCAGC 5 cut(s) 187, 469, 1226, 1230, 1454
BstV2I GAAGAC 2 cut(s) 145, 903
BsuRI GGCC 6 cut(s) 44, 341, 518, 861, 1131, 1192
BtgI CCRYGG 2 cut(s) 333, 657
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 1329
BtsIMutI CAGTG 4 cut(s) 465, 924, 1024, 1329
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 3 cut(s) 169, 242, 1194
CciI TCATGA 1 cut(s) 1272
CseI GACGC 1 cut(s) 173
Csp6I GTAC 3 cut(s) 732, 1009, 1278
CviAII CATG 5 cut(s) 124, 334, 932, 1138, 1273
CviQI GTAC 3 cut(s) 732, 1009, 1278
DdeI CTNAG 2 cut(s) 578, 1016
DpnI GATC 4 cut(s) 362, 677, 752, 852
DpnII GATC 4 cut(s) 360, 675, 750, 850
DraIII CACNNNGTG 1 cut(s) 1378
EaeI YGGCCR 3 cut(s) 42, 339, 1129
Eam1104I CTCTTC 3 cut(s) 592, 897, 1437
EarI CTCTTC 3 cut(s) 592, 897, 1437
Eco130I CCWWGG 4 cut(s) 333, 442, 513, 756
Eco147I AGGCCT 2 cut(s) 518, 861
Eco24I GRGCYC 1 cut(s) 609
EcoRII CCWGG 1 cut(s) 1200
EcoT14I CCWWGG 4 cut(s) 333, 442, 513, 756
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 4 cut(s) 333, 442, 513, 756
Esp3I CGTCTC 1 cut(s) 1139
FaeI CATG 5 cut(s) 127, 337, 935, 1141, 1276
FaqI GGGAC 4 cut(s) 112, 1068, 1363, 1543
FatI CATG 5 cut(s) 123, 333, 931, 1137, 1272
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 2 cut(s) 675, 850
Fnu4HI GCNGC 5 cut(s) 201, 483, 1215, 1244, 1468
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 5 cut(s) 201, 483, 1215, 1244, 1468
FspBI CTAG 5 cut(s) 551, 812, 825, 1004, 1538
GluI GCNGC 5 cut(s) 201, 483, 1215, 1244, 1468
HaeIII GGCC 6 cut(s) 44, 341, 518, 861, 1131, 1192
HapII CCGG 5 cut(s) 192, 305, 342, 609, 1302
HgaI GACGC 1 cut(s) 173
Hin1II CATG 5 cut(s) 127, 337, 935, 1141, 1276
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 1 cut(s) 663
HpaII CCGG 5 cut(s) 192, 305, 342, 609, 1302
HphI GGTGA 4 cut(s) 73, 322, 398, 670
Hpy166II GTNNAC 3 cut(s) 832, 1009, 1299
Hpy188I TCNGA 3 cut(s) 163, 579, 855
Hpy188III TCNNGA 7 cut(s) 109, 798, 1042, 1071, 1151, 1167, 1273
Hpy8I GTNNAC 3 cut(s) 832, 1009, 1299
HpyAV CCTTC 5 cut(s) 94, 221, 795, 1152, 1507
HpyCH4III ACNGT 4 cut(s) 266, 370, 919, 1534
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 258, 867, 1214, 1223
HpyF3I CTNAG 2 cut(s) 578, 1016
Hsp92II CATG 5 cut(s) 127, 337, 935, 1141, 1276
KpnI GGTACC 1 cut(s) 735
Ksp22I TGATCA 2 cut(s) 675, 850
Kzo9I GATC 4 cut(s) 360, 675, 750, 850
LguI GCTCTTC 1 cut(s) 1437
LmnI GCTCC 4 cut(s) 604, 807, 1229, 1304
Lsp1109I GCAGC 5 cut(s) 187, 469, 1226, 1230, 1454
LweI GCATC 1 cut(s) 1536
MaeI CTAG 5 cut(s) 551, 812, 825, 1004, 1538
MaeIII GTNAC 3 cut(s) 283, 437, 1484
MalI GATC 4 cut(s) 362, 677, 752, 852
MboI GATC 4 cut(s) 360, 675, 750, 850
MfeI CAATTG 1 cut(s) 1026
MhlI GDGCHC 3 cut(s) 609, 834, 1301
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 8 cut(s) 272, 632, 780, 835, 1026, 1308, 1357, 1582
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 9 cut(s) 158, 169, 239, 593, 617, 886, 898, 943, 1067
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 3 cut(s) 32, 635, 927
MslI CAYNNNNRTG 2 cut(s) 335, 590
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 5 cut(s) 192, 305, 342, 609, 1302
MspR9I CCNGG 2 cut(s) 609, 1202
MunI CAATTG 1 cut(s) 1026
Mva1269I GAATGC 1 cut(s) 1196
MvaI CCWGG 1 cut(s) 1202
MwoI GCNNNNNNNGC 5 cut(s) 164, 258, 867, 1214, 1223
NciI CCSGG 1 cut(s) 609
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 4 cut(s) 360, 675, 750, 850
NlaIII CATG 5 cut(s) 127, 337, 935, 1141, 1276
NlaIV GGNNCC 4 cut(s) 570, 606, 733, 809
NmeAIII GCCGAG 1 cut(s) 228
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 1272
PceI AGGCCT 2 cut(s) 518, 861
PciSI GCTCTTC 1 cut(s) 1437
PctI GAATGC 1 cut(s) 1196
PfeI GAWTC 1 cut(s) 663
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 5 cut(s) 202, 484, 1216, 1245, 1469
PshBI ATTAAT 1 cut(s) 635
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 1200
PspGI CCWGG 1 cut(s) 1200
PspN4I GGNNCC 4 cut(s) 570, 606, 733, 809
PsrI GAACNNNNNNTAC 2 cut(s) 1504, 1536
RsaI GTAC 3 cut(s) 733, 1010, 1279
RsaNI GTAC 3 cut(s) 732, 1009, 1278
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1437
SaqAI TTAA 3 cut(s) 32, 635, 927
SatI GCNGC 5 cut(s) 201, 483, 1215, 1244, 1468
Sau3AI GATC 4 cut(s) 360, 675, 750, 850
ScrFI CCNGG 2 cut(s) 609, 1202
SduI GDGCHC 3 cut(s) 609, 834, 1301
SfaNI GCATC 1 cut(s) 1536
SfcI CTRYAG 3 cut(s) 420, 915, 1335
SfuI TTCGAA 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 3 cut(s) 107, 863, 1165
SmoI CTYRAG 3 cut(s) 107, 863, 1165
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 8 cut(s) 272, 632, 780, 835, 1026, 1308, 1357, 1582
SseBI AGGCCT 2 cut(s) 518, 861
SsiI CCGC 2 cut(s) 735, 1281
SspI AATATT 1 cut(s) 321
SspMI CTAG 5 cut(s) 551, 812, 825, 1004, 1538
StuI AGGCCT 2 cut(s) 518, 861
StyD4I CCNGG 2 cut(s) 607, 1200
StyI CCWWGG 4 cut(s) 333, 442, 513, 756
TaaI ACNGT 4 cut(s) 266, 370, 919, 1534
TaqI TCGA 1 cut(s) 391
TasI AATT 8 cut(s) 272, 632, 780, 835, 1026, 1308, 1357, 1582
TatI WGTACW 1 cut(s) 1008
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 3 cut(s) 32, 635, 927
Tru9I TTAA 3 cut(s) 32, 635, 927
TscAI CASTG 4 cut(s) 472, 924, 1024, 1329
TseI GCWGC 5 cut(s) 200, 482, 1214, 1243, 1467
TspDTI ATGAA 4 cut(s) 1104, 1470, 1489, 1575
TspGWI ACGGA 2 cut(s) 84, 390
TspRI CASTG 4 cut(s) 472, 924, 1024, 1329
Van91I CCANNNNNTGG 1 cut(s) 51
VneI GTGCAC 2 cut(s) 830, 1297
VspI ATTAAT 1 cut(s) 635
XapI RAATTY 2 cut(s) 1357, 1582
XspI CTAG 5 cut(s) 551, 812, 825, 1004, 1538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.