Rh5DG504400

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001224
Physical Location & Seq
Forward (+)
78874220 .. 78876667
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG504400.1

Sequence Viewer

Length: 1605 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGGCATCATATGGCTTCAATCCATAAATGGAACAAACACAACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTCCATGTCCCAACTACAAATCAACAACCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATGATCGGATCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATCAACACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTCTGTTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCAAGAAACATGGCAACGGGGTTCAATTTAATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAGTGATAGGCACCGGAGTATTCCTACTGATGCCCTTGGTAATCCCAATTGTGGAAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATAATGAGGTTGGTGAAGTGCAGGTTCATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGACGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTTTATTTTTTCAGTGCAACAATTGGGCTCGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGACTATTTCTTCGCAAGAAGCAAGTATATGATTTCAAGGCCTGCATTCCTAATGGCATTAGTGGCACCAATAGCAGGAGTTTTTTTCTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGCTATTCGGGACCAAAAAATTCTCAATAAATCACAATGTGGTGGTTGCGAATATCTCAATCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTGTTTATCGCAAGGAAGGAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTACAGAAGCACCTTCATTATCTGGGGTAGTCTTTGTACGTTAGGGACTGTCCTAGCTTTAACGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

534

Amino Acids

58.61

Weight (kDa)

8.88

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 253 2.4e-71 Nodulin-like
NFD4_C PF23262 318 - 531 2.5e-103 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 901, 928
AccB1I GGYRCC 3 cut(s) 568, 719, 1231
AciI CCGC 1 cut(s) 1296
AclWI GGATC 3 cut(s) 247, 368, 697
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 1 cut(s) 1373
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1393
AfaI GTAC 4 cut(s) 821, 904, 1294, 1537
AfiI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
AgsI TTSAA 4 cut(s) 59, 631, 1060, 1203
AhlI ACTAGT 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 5 cut(s) 20, 1271, 1357, 1482, 1556
AluI AGCT 5 cut(s) 20, 1271, 1357, 1482, 1556
Alw21I GWGCWC 2 cut(s) 514, 1316
Alw26I GTCTC 3 cut(s) 559, 1154, 1282
Alw44I GTGCAC 1 cut(s) 1312
AlwI GGATC 3 cut(s) 247, 368, 697
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 516, 847, 1144, 1205
ApaLI GTGCAC 1 cut(s) 1312
ApeKI GCWGC 3 cut(s) 200, 482, 1482
ApoI RAATTY 1 cut(s) 1373
ArsI GACNNNNNNTTYG 2 cut(s) 1160, 1192
AspS9I GGNCC 1 cut(s) 1365
AsuHPI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 1365
BaeGI GKGCMC 1 cut(s) 1316
BanI GGYRCC 3 cut(s) 568, 719, 1231
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 2 cut(s) 514, 1316
BbvI GCAGC 3 cut(s) 187, 469, 1469
BccI CCATC 3 cut(s) 786, 979, 1469
BceAI ACGGC 3 cut(s) 280, 656, 1131
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 559, 1154, 1282
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
BfuAI ACCTGC 2 cut(s) 901, 928
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 201, 483, 1483
BlsI GCNGC 3 cut(s) 202, 484, 1484
Bme18I GGWCC 1 cut(s) 1365
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 1365
BmiI GGNNCC 6 cut(s) 570, 606, 721, 797, 1233, 1366
BmsI GCATC 2 cut(s) 54, 729
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 2 cut(s) 288, 1338
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 2 cut(s) 92, 872
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 333, 442, 744, 1065
BsaWI WCCGGW 2 cut(s) 659, 722
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 760, 1241
Bse1I ACTGG 2 cut(s) 681, 1321
Bse3DI GCAATG 2 cut(s) 195, 651
BseDI CCNNGG 4 cut(s) 333, 442, 744, 1065
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 681, 1321
BseRI GAGGAG 1 cut(s) 980
BseSI GKGCMC 1 cut(s) 1316
BseXI GCAGC 3 cut(s) 187, 469, 1469
BseYI CCCAGC 1 cut(s) 1267
BsgI GTGCAG 2 cut(s) 929, 956
BshFI GGCC 4 cut(s) 518, 849, 1146, 1207
BshNI GGYRCC 3 cut(s) 568, 719, 1231
BsiHKAI GWGCWC 2 cut(s) 514, 1316
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 192, 660, 723
BslFI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
BslI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
BsmAI GTCTC 3 cut(s) 559, 1154, 1282
BsmBI CGTCTC 2 cut(s) 1154, 1282
BsmFI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 4 cut(s) 518, 849, 1146, 1207
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 514, 1050, 1316
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 6 cut(s) 234, 239, 360, 689, 696, 838
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 1 cut(s) 1296
BspANI GGCC 4 cut(s) 518, 849, 1146, 1207
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 6 cut(s) 570, 606, 721, 797, 1233, 1366
BspMI ACCTGC 2 cut(s) 901, 928
BspPI GGATC 3 cut(s) 247, 368, 697
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 3 cut(s) 568, 719, 1231
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1321
BssECI CCNNGG 4 cut(s) 333, 442, 744, 1065
BssMI GATC 6 cut(s) 234, 239, 360, 689, 696, 838
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 1049
BssT1I CCWWGG 4 cut(s) 333, 442, 744, 1065
Bst2BI CACGAG 1 cut(s) 1049
Bst4CI ACNGT 3 cut(s) 370, 907, 1549
Bst6I CTCTTC 1 cut(s) 885
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 343, 1209
BstDEI CTNAG 2 cut(s) 703, 1098
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 6 cut(s) 237, 242, 363, 692, 699, 841
BstMAI GTCTC 3 cut(s) 559, 1154, 1282
BstMBI GATC 6 cut(s) 234, 239, 360, 689, 696, 838
BstMWI GCNNNNNNNGC 5 cut(s) 42, 164, 855, 1229, 1238
BstSLI GKGCMC 1 cut(s) 1316
BstV1I GCAGC 3 cut(s) 187, 469, 1469
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 518, 849, 1146, 1207
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 1344
BtsIMutI CAGTG 5 cut(s) 484, 912, 1039, 1314, 1344
BveI ACCTGC 2 cut(s) 901, 928
Cac8I GCNNGC 2 cut(s) 343, 1209
CciI TCATGA 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 1365
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 4 cut(s) 820, 903, 1293, 1536
CviAII CATG 6 cut(s) 124, 232, 334, 616, 947, 1153
CviQI GTAC 4 cut(s) 820, 903, 1293, 1536
DdeI CTNAG 2 cut(s) 703, 1098
DpnI GATC 6 cut(s) 236, 241, 362, 691, 698, 840
DpnII GATC 6 cut(s) 234, 239, 360, 689, 696, 838
DraIII CACNNNGTG 1 cut(s) 1393
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
Eco130I CCWWGG 4 cut(s) 333, 442, 744, 1065
Eco147I AGGCCT 2 cut(s) 518, 1207
Eco24I GRGCYC 1 cut(s) 1050
Eco47I GGWCC 1 cut(s) 1365
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 4 cut(s) 333, 442, 744, 1065
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 4 cut(s) 333, 442, 744, 1065
Esp3I CGTCTC 2 cut(s) 1154, 1282
FaeI CATG 6 cut(s) 127, 235, 337, 619, 950, 1156
FaqI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
FatI CATG 6 cut(s) 123, 231, 333, 615, 946, 1152
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 3 cut(s) 201, 483, 1483
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 3 cut(s) 201, 483, 1483
FspBI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
GluI GCNGC 3 cut(s) 201, 483, 1483
GsaI CCCAGC 1 cut(s) 1271
GsuI CTGGAG 2 cut(s) 288, 1338
HaeIII GGCC 4 cut(s) 518, 849, 1146, 1207
HapII CCGG 3 cut(s) 192, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 127, 235, 337, 619, 950, 1156
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 663, 1082
HpaII CCGG 3 cut(s) 192, 660, 723
HphI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 941
Hpy166II GTNNAC 2 cut(s) 820, 1314
Hpy188I TCNGA 4 cut(s) 163, 239, 773, 843
Hpy188III TCNNGA 7 cut(s) 109, 232, 786, 1057, 1086, 1166, 1363
Hpy8I GTNNAC 2 cut(s) 820, 1314
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 6 cut(s) 94, 221, 1141, 1167, 1457, 1522
HpyCH4III ACNGT 3 cut(s) 370, 907, 1549
HpyCH4IV ACGT 3 cut(s) 980, 1275, 1538
HpyCH4V TGCA 9 cut(s) 455, 910, 937, 991, 1037, 1211, 1314, 1337, 1571
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 164, 855, 1229, 1238
HpyF3I CTNAG 2 cut(s) 703, 1098
HpySE526I ACGT 3 cut(s) 980, 1275, 1538
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 127, 235, 337, 619, 950, 1156
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 6 cut(s) 234, 239, 360, 689, 696, 838
LmnI GCTCC 3 cut(s) 604, 795, 1319
Lsp1109I GCAGC 3 cut(s) 187, 469, 1469
LweI GCATC 2 cut(s) 54, 729
MaeI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
MaeII ACGT 3 cut(s) 980, 1275, 1538
MaeIII GTNAC 4 cut(s) 283, 437, 976, 1499
MalI GATC 6 cut(s) 236, 241, 362, 691, 698, 840
MboI GATC 6 cut(s) 234, 239, 360, 689, 696, 838
MfeI CAATTG 2 cut(s) 756, 1041
MhlI GDGCHC 3 cut(s) 514, 1050, 1316
MluCI AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 2 cut(s) 186, 338
MseI TTAA 3 cut(s) 32, 635, 1559
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 3 cut(s) 192, 660, 723
MunI CAATTG 2 cut(s) 756, 1041
Mva1269I GAATGC 1 cut(s) 1211
MwoI GCNNNNNNNGC 5 cut(s) 42, 164, 855, 1229, 1238
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 6 cut(s) 234, 239, 360, 689, 696, 838
NlaIII CATG 6 cut(s) 127, 235, 337, 619, 950, 1156
NlaIV GGNNCC 6 cut(s) 570, 606, 721, 797, 1233, 1366
NmuCI GTSAC 1 cut(s) 976
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 231
PceI AGGCCT 2 cut(s) 518, 1207
PctI GAATGC 1 cut(s) 1211
PfeI GAWTC 1 cut(s) 663
PkrI GCNGC 3 cut(s) 202, 484, 1484
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 1267
PspN4I GGNNCC 6 cut(s) 570, 606, 721, 797, 1233, 1366
PspPI GGNCC 1 cut(s) 1365
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 4 cut(s) 821, 904, 1294, 1537
RsaNI GTAC 4 cut(s) 820, 903, 1293, 1536
RseI CAYNNNNRTG 2 cut(s) 335, 590
SaqAI TTAA 3 cut(s) 32, 635, 1559
SatI GCNGC 3 cut(s) 201, 483, 1483
Sau3AI GATC 6 cut(s) 234, 239, 360, 689, 696, 838
Sau96I GGNCC 1 cut(s) 1365
SchI GAGTC 1 cut(s) 1091
SduI GDGCHC 3 cut(s) 514, 1050, 1316
SfaNI GCATC 2 cut(s) 54, 729
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 1 cut(s) 1365
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 2 cut(s) 107, 851
SmoI CTYRAG 2 cut(s) 107, 851
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
SseBI AGGCCT 2 cut(s) 518, 1207
SsiI CCGC 1 cut(s) 1296
SspI AATATT 1 cut(s) 321
SspMI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
StuI AGGCCT 2 cut(s) 518, 1207
StyI CCWWGG 4 cut(s) 333, 442, 744, 1065
TaaI ACNGT 3 cut(s) 370, 907, 1549
TaiI ACGT 3 cut(s) 983, 1278, 1541
TaqI TCGA 1 cut(s) 391
TasI AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
TatI WGTACW 2 cut(s) 819, 902
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 3 cut(s) 32, 635, 1559
Tru9I TTAA 3 cut(s) 32, 635, 1559
TscAI CASTG 5 cut(s) 484, 912, 1039, 1321, 1344
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 3 cut(s) 200, 482, 1482
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 4 cut(s) 905, 932, 1485, 1504
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 5 cut(s) 484, 912, 1039, 1321, 1344
VneI GTGCAC 1 cut(s) 1312
VpaK11BI GGWCC 1 cut(s) 1365
XapI RAATTY 1 cut(s) 1373
XspI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.