Rh5AG411500

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001224
Physical Location & Seq
Reverse (-)
71086523 .. 71088686
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG411500.1

Sequence Viewer

Length: 1605 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGGCATCATATGGCTTCAATCCATAAATGGAACAAACACAACCTTCCCTGCTTACTCATCCCAACTCAAGAACAGTCTCTCCATGTCCCAAATACAAATCAACAATCTCGTCTTCGCCTCTGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATAATCGGATCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATCAATACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGCTTCTCCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTTCCTTTTCTTCTTCACCTCATAAGAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGCTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCAAGAAACATGGCAACCGGGTTCAATTTAATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAGTGATAGGCACCGGAGTATTCCTACTGATGCCCTTGGTAATCCCAATTGTGGAAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTGGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAGCATAGTACAGTGCAGGTTCACAATGAGGTTGGTGAAGTACAGGTTCATCATGGGATTGGTGTTAAGGAGGAGATTGGAGTGACGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTTTATTTTTTCAGTGCAACAATTGGGCTCGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGATTATTTCTTCGCAAGAAGCAAGTATATGATTTCAAGGCCTGCATTCCTAATGGCATTAGCGGCACCAATAGCAGGAGTTTTTTTCTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTTCAGTGTCCATAACCACAGAGCTATTCGGGACCAAAAAATTCTCAATAAATCACAATGTGGTGGTTGCGAATATCTCAATCGGGTCCTTTCTTTTTGGCTACTCATCTGCTCTTCTTTATAGTAAGGAAGGAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTACAGAATCACCTTCATTATCTGGGGTAGTCTTTGTACGTTTGGGACTGTCCTAGCTTTAACGCTATGTGCAAGAACAAGAAAGTTCTACTCCAAAAGACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

534

Amino Acids

58.43

Weight (kDa)

8.66

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 253 2.7e-70 Nodulin-like
NFD4_C PF23262 318 - 531 9.7e-101 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
AccB1I GGYRCC 3 cut(s) 568, 719, 1231
AciI CCGC 3 cut(s) 401, 1229, 1296
AclWI GGATC 3 cut(s) 247, 368, 697
AcoI YGGCCR 1 cut(s) 1144
AcsI RAATTY 1 cut(s) 1373
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1393
AfaI GTAC 5 cut(s) 821, 904, 936, 1294, 1537
AfiI CCNNNNNNNGG 2 cut(s) 760, 1241
AgsI TTSAA 4 cut(s) 59, 631, 1060, 1203
AhlI ACTAGT 1 cut(s) 550
AjnI CCWGG 1 cut(s) 849
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 5 cut(s) 20, 1271, 1357, 1482, 1556
AluI AGCT 5 cut(s) 20, 1271, 1357, 1482, 1556
Alw21I GWGCWC 2 cut(s) 514, 1316
Alw26I GTCTC 3 cut(s) 122, 1154, 1282
Alw44I GTGCAC 1 cut(s) 1312
AlwI GGATC 3 cut(s) 247, 368, 697
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 516, 847, 1144, 1205
ApaLI GTGCAC 1 cut(s) 1312
ApeKI GCWGC 2 cut(s) 200, 1482
ApoI RAATTY 1 cut(s) 1373
AspS9I GGNCC 2 cut(s) 1365, 1419
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 6 cut(s) 322, 489, 589, 670, 941, 1501
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 2 cut(s) 1365, 1419
BaeGI GKGCMC 1 cut(s) 1316
BanI GGYRCC 3 cut(s) 568, 719, 1231
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 2 cut(s) 514, 1316
BbvI GCAGC 2 cut(s) 187, 1469
BccI CCATC 3 cut(s) 786, 979, 1469
BceAI ACGGC 3 cut(s) 280, 656, 1131
BciT130I CCWGG 1 cut(s) 851
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 3 cut(s) 122, 1154, 1282
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 201, 1230, 1483
BlsI GCNGC 3 cut(s) 202, 1231, 1484
Bme1390I CCNGG 2 cut(s) 625, 851
Bme18I GGWCC 2 cut(s) 1365, 1419
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 1365, 1419
BmiI GGNNCC 7 cut(s) 570, 606, 721, 797, 1233, 1366, 1420
BmrFI CCNGG 2 cut(s) 625, 851
BmsI GCATC 2 cut(s) 54, 729
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 3 cut(s) 288, 872, 1338
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 625
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 333, 442, 744, 1065
BsaWI WCCGGW 2 cut(s) 659, 722
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1241
Bse1I ACTGG 2 cut(s) 681, 1321
Bse3DI GCAATG 2 cut(s) 195, 651
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 4 cut(s) 333, 442, 744, 1065
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 2 cut(s) 760, 1241
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 681, 1321
BseRI GAGGAG 2 cut(s) 908, 980
BseSI GKGCMC 1 cut(s) 1316
BseXI GCAGC 2 cut(s) 187, 1469
BseYI CCCAGC 1 cut(s) 1267
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 4 cut(s) 518, 849, 1146, 1207
BshNI GGYRCC 3 cut(s) 568, 719, 1231
BsiHKAI GWGCWC 2 cut(s) 514, 1316
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 192, 624, 660, 723
BslFI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
BslI CCNNNNNNNGG 2 cut(s) 760, 1241
BsmAI GTCTC 3 cut(s) 122, 1154, 1282
BsmBI CGTCTC 2 cut(s) 1154, 1282
BsmFI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 4 cut(s) 518, 849, 1146, 1207
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 514, 1050, 1316
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 5 cut(s) 239, 360, 689, 696, 838
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 3 cut(s) 401, 1229, 1296
BspANI GGCC 4 cut(s) 518, 849, 1146, 1207
BspLI GGNNCC 7 cut(s) 570, 606, 721, 797, 1233, 1366, 1420
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 3 cut(s) 247, 368, 697
BspQI GCTCTTC 1 cut(s) 1452
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 3 cut(s) 568, 719, 1231
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1321
BssECI CCNNGG 4 cut(s) 333, 442, 744, 1065
BssMI GATC 5 cut(s) 239, 360, 689, 696, 838
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 1049
BssT1I CCWWGG 4 cut(s) 333, 442, 744, 1065
Bst2BI CACGAG 1 cut(s) 1049
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 4 cut(s) 116, 370, 907, 1549
Bst6I CTCTTC 2 cut(s) 885, 1452
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 343, 1209
BstDEI CTNAG 2 cut(s) 703, 1098
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 5 cut(s) 242, 363, 692, 699, 841
BstMAI GTCTC 3 cut(s) 122, 1154, 1282
BstMBI GATC 5 cut(s) 239, 360, 689, 696, 838
BstMWI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 2 cut(s) 623, 849
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 1316
BstV1I GCAGC 2 cut(s) 187, 1469
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 518, 849, 1146, 1207
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 5 cut(s) 484, 912, 1039, 1314, 1344
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 343, 1209
Cfr13I GGNCC 2 cut(s) 1365, 1419
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 5 cut(s) 820, 903, 935, 1293, 1536
CviAII CATG 5 cut(s) 124, 334, 616, 947, 1153
CviQI GTAC 5 cut(s) 820, 903, 935, 1293, 1536
DdeI CTNAG 2 cut(s) 703, 1098
DpnI GATC 5 cut(s) 241, 362, 691, 698, 840
DpnII GATC 5 cut(s) 239, 360, 689, 696, 838
DraIII CACNNNGTG 1 cut(s) 1393
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 2 cut(s) 885, 1452
EarI CTCTTC 2 cut(s) 885, 1452
Eco130I CCWWGG 4 cut(s) 333, 442, 744, 1065
Eco147I AGGCCT 2 cut(s) 518, 1207
Eco24I GRGCYC 1 cut(s) 1050
Eco47I GGWCC 2 cut(s) 1365, 1419
Eco88I CYCGRG 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 1419
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 4 cut(s) 333, 442, 744, 1065
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 4 cut(s) 333, 442, 744, 1065
Esp3I CGTCTC 2 cut(s) 1154, 1282
FaeI CATG 5 cut(s) 127, 337, 619, 950, 1156
FaqI GGGAC 5 cut(s) 112, 438, 1083, 1378, 1558
FatI CATG 5 cut(s) 123, 333, 615, 946, 1152
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 3 cut(s) 201, 1230, 1483
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 3 cut(s) 201, 1230, 1483
FspBI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
GluI GCNGC 3 cut(s) 201, 1230, 1483
GsaI CCCAGC 1 cut(s) 1271
GsuI CTGGAG 3 cut(s) 288, 872, 1338
HaeIII GGCC 4 cut(s) 518, 849, 1146, 1207
HapII CCGG 4 cut(s) 192, 624, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 127, 337, 619, 950, 1156
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 3 cut(s) 663, 1082, 1506
HpaII CCGG 4 cut(s) 192, 624, 660, 723
HphI GGTGA 6 cut(s) 322, 489, 589, 670, 941, 1501
Hpy166II GTNNAC 3 cut(s) 820, 916, 1314
Hpy188I TCNGA 4 cut(s) 163, 239, 773, 843
Hpy188III TCNNGA 6 cut(s) 109, 786, 1057, 1086, 1166, 1363
Hpy8I GTNNAC 3 cut(s) 820, 916, 1314
HpyAV CCTTC 6 cut(s) 94, 221, 1141, 1167, 1457, 1522
HpyCH4III ACNGT 4 cut(s) 116, 370, 907, 1549
HpyCH4IV ACGT 3 cut(s) 980, 1275, 1538
HpyCH4V TGCA 7 cut(s) 455, 910, 991, 1037, 1211, 1314, 1571
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
HpyF3I CTNAG 2 cut(s) 703, 1098
HpySE526I ACGT 3 cut(s) 980, 1275, 1538
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 127, 337, 619, 950, 1156
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 5 cut(s) 239, 360, 689, 696, 838
LguI GCTCTTC 1 cut(s) 1452
LmnI GCTCC 4 cut(s) 604, 795, 895, 1319
Lsp1109I GCAGC 2 cut(s) 187, 1469
LweI GCATC 2 cut(s) 54, 729
MaeI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
MaeII ACGT 3 cut(s) 980, 1275, 1538
MaeIII GTNAC 4 cut(s) 283, 437, 976, 1499
MalI GATC 5 cut(s) 241, 362, 691, 698, 840
MboI GATC 5 cut(s) 239, 360, 689, 696, 838
MfeI CAATTG 2 cut(s) 756, 1041
MhlI GDGCHC 3 cut(s) 514, 1050, 1316
MluCI AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 2 cut(s) 338, 433
MnlI CCTC 9 cut(s) 19, 169, 239, 510, 874, 886, 916, 958, 1082
MseI TTAA 4 cut(s) 32, 635, 960, 1559
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 4 cut(s) 192, 624, 660, 723
MspR9I CCNGG 2 cut(s) 625, 851
MunI CAATTG 2 cut(s) 756, 1041
Mva1269I GAATGC 1 cut(s) 1211
MvaI CCWGG 1 cut(s) 851
MwoI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 5 cut(s) 239, 360, 689, 696, 838
NlaIII CATG 5 cut(s) 127, 337, 619, 950, 1156
NlaIV GGNNCC 7 cut(s) 570, 606, 721, 797, 1233, 1366, 1420
NmuCI GTSAC 1 cut(s) 976
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PceI AGGCCT 2 cut(s) 518, 1207
PciSI GCTCTTC 1 cut(s) 1452
PctI GAATGC 1 cut(s) 1211
PfeI GAWTC 2 cut(s) 663, 1506
PkrI GCNGC 3 cut(s) 202, 1231, 1484
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PpuMI RGGWCCY 1 cut(s) 1419
Psp5II RGGWCCY 1 cut(s) 1419
Psp6I CCWGG 1 cut(s) 849
PspFI CCCAGC 1 cut(s) 1267
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 7 cut(s) 570, 606, 721, 797, 1233, 1366, 1420
PspPI GGNCC 2 cut(s) 1365, 1419
PspPPI RGGWCCY 1 cut(s) 1419
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 5 cut(s) 821, 904, 936, 1294, 1537
RsaNI GTAC 5 cut(s) 820, 903, 935, 1293, 1536
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1452
SaqAI TTAA 4 cut(s) 32, 635, 960, 1559
SatI GCNGC 3 cut(s) 201, 1230, 1483
Sau3AI GATC 5 cut(s) 239, 360, 689, 696, 838
Sau96I GGNCC 2 cut(s) 1365, 1419
SchI GAGTC 1 cut(s) 1091
ScrFI CCNGG 2 cut(s) 625, 851
SduI GDGCHC 3 cut(s) 514, 1050, 1316
SfaNI GCATC 2 cut(s) 54, 729
SfcI CTRYAG 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 2 cut(s) 1365, 1419
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
SseBI AGGCCT 2 cut(s) 518, 1207
SsiI CCGC 3 cut(s) 401, 1229, 1296
SspI AATATT 1 cut(s) 321
SspMI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
StuI AGGCCT 2 cut(s) 518, 1207
StyD4I CCNGG 2 cut(s) 623, 849
StyI CCWWGG 4 cut(s) 333, 442, 744, 1065
TaaI ACNGT 4 cut(s) 116, 370, 907, 1549
TaiI ACGT 3 cut(s) 983, 1278, 1541
TaqI TCGA 1 cut(s) 391
TasI AATT 6 cut(s) 272, 631, 756, 1041, 1323, 1373
TatI WGTACW 3 cut(s) 819, 902, 934
TauI GCSGC 1 cut(s) 1232
TfiI GAWTC 2 cut(s) 663, 1506
Tru1I TTAA 4 cut(s) 32, 635, 960, 1559
Tru9I TTAA 4 cut(s) 32, 635, 960, 1559
TscAI CASTG 5 cut(s) 484, 912, 1039, 1321, 1344
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 2 cut(s) 200, 1482
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 932, 1485, 1504
TspRI CASTG 5 cut(s) 484, 912, 1039, 1321, 1344
VneI GTGCAC 1 cut(s) 1312
VpaK11BI GGWCC 2 cut(s) 1365, 1419
XapI RAATTY 1 cut(s) 1373
XspI CTAG 6 cut(s) 38, 224, 551, 800, 1019, 1553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.