Rmu_sc0003673.1_g000010

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003673.1
OG0001224
Physical Location & Seq
Forward (+)
23258 .. 25408
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003673.1_g000010.1.cds

Sequence Viewer

Length: 1845 bp
atgtcttcatcctcatcaaaagctttccaatggttaagccttgtgggcatcatatggcttcaatccataaacggaacgaacaccaccttccctgcttactcatcccaactcaagaacactctcttcctctcccaactacaaatcaacaatctcgtcttcgcctccgacgctgggaaactctttggttggttttccggcattgctgctatttaccttcccctctggctagtcctcattatcggttcacttctcgggttgattggctacggtgtccaatttctattcgtaactaaccaaatctcatctccagcatattggcaaatattcttgctcagcatggtggccggaaatagtgtctgttggatcaacaccgtttgctatgttgtttgcattcgaaactttccgtttcaccaacaggttgctgtgggactaacaacaagttaccagggattgagtgcaaaaatctacacagatatcgttggtgctgctttttcttcttcacctcataagagagccaaggcctatcttcttctaaactctctcctgcctttactagtctgtgttgtagtggcaccatttgtgagagacattgatgtgggaagaggggagcccagaaatatggcagcggggttcaaattaatgtttgtggtaacaattgccaccggaatctatgccgtgatcaccagtttaggatcaatgtcgtctatccacttatcgccattgcagggtgtgataggtaccgtattgttcctactgatccccatggtcatcccaattgtggaaaaaatcggagaaaaattatcaagaaggtggagcctagacagagagaacagagtgcatgacttcagcatagaagatcagaaaggccttgagagcatagagagtggtgtagtgaaagaggaagaagaagaggaacatagtacagtgcaggttcataatgaggttggtgaagtacaggttcatcatgggattggtgttagagaggagattggagtgacgttgatggtgcaaaggatagatttctggttatatttcctagtttattttttcagtgcaacaattgggctcgtgttcttgaacaccttgggacagatagcagagtcaagaggctcatctaagacttcttctctggtttctttatcttcttcatttgggttctttggccgtctcatgacttcacttctggactatttcttctcaagaagcaagtacatgatttcaaggcctgcattcctagtgacattagcggcaccaatagcaggagttttcttcttgcttctcaacccagctaacgtctcccttatcatcagtaccgccattataggagtgtgcactggagcaattacttctattgcagtgtccataaccacagagctattcgggaccaaaaaattctcaataaatcacaatgtggtggttgcgaatatctcagtcgggtcgtttctttttggctactcatctgctcttctttatagtaaggaaggaaatgaagatggtagctgcttgggaatggcttgcagtgacattattgacgaacccaaaatctactcctctgctgaaccggaagactggtgtgggaaagccgaccaaacatctgcccatgtcctcactgcctcagctatatccttgacaacagatgctgcctctcacatcagcacggatttgatcattgctgtatctttcgcatattgtcacagaagatgtggaatcaacagtgattgcaaacggtacggtacaccaagttcatcagtcctcgtgtatcgtttcccgattagacaccagaggaagtccaaaataaaacagttgaaaaactcaattggtttaggaattacaagcataaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

614

Amino Acids

67.02

Weight (kDa)

8.37

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 919
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 3 cut(s) 571, 737, 1249
AciI CCGC 3 cut(s) 626, 1247, 1314
AclWI GGATC 3 cut(s) 371, 700, 751
AcoI YGGCCR 2 cut(s) 342, 1162
AcsI RAATTY 1 cut(s) 1391
AcuI CTGAAG 1 cut(s) 829
AdeI CACNNNGTG 1 cut(s) 1411
AfaI GTAC 7 cut(s) 739, 922, 954, 1211, 1312, 1730, 1735
AfiI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
AgsI TTSAA 5 cut(s) 62, 634, 1078, 1221, 1807
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 444
AluBI AGCT 5 cut(s) 23, 1289, 1375, 1500, 1619
AluI AGCT 5 cut(s) 23, 1289, 1375, 1500, 1619
Alw21I GWGCWC 1 cut(s) 1334
Alw26I GTCTC 3 cut(s) 579, 1172, 1300
Alw44I GTGCAC 1 cut(s) 1330
AlwI GGATC 3 cut(s) 371, 700, 751
AlwNI CAGNNNCTG 1 cut(s) 1640
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 342, 519, 865, 1162, 1223
ApaLI GTGCAC 1 cut(s) 1330
ApeKI GCWGC 5 cut(s) 203, 485, 623, 1500, 1640
ApoI RAATTY 1 cut(s) 1391
AseI ATTAAT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 1383
AsuHPI GGTGA 4 cut(s) 401, 492, 673, 959
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1383
BaeGI GKGCMC 1 cut(s) 1334
BanI GGYRCC 3 cut(s) 571, 737, 1249
BanII GRGCYC 2 cut(s) 612, 1068
BauI CACGAG 2 cut(s) 1067, 1754
BbsI GAAGAC 2 cut(s) 148, 1572
Bbv12I GWGCWC 1 cut(s) 1334
BbvCI CCTCAGC 1 cut(s) 1615
BbvI GCAGC 5 cut(s) 190, 472, 635, 1487, 1627
BccI CCATC 2 cut(s) 997, 1487
BceAI ACGGC 2 cut(s) 659, 1149
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 2 cut(s) 678, 1665
BcoDI GTCTC 3 cut(s) 579, 1172, 1300
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 5 cut(s) 227, 554, 818, 1037, 1235
BfuAI ACCTGC 1 cut(s) 919
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 6 cut(s) 204, 486, 624, 1248, 1501, 1641
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 6 cut(s) 205, 487, 625, 1249, 1502, 1642
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 1383
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1383
BmiI GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 57, 1627
BpiI GAAGAC 2 cut(s) 148, 1572
BpmI CTGGAG 2 cut(s) 291, 1356
Bpu10I CCTNAGC 1 cut(s) 1615
Bpu1102I GCTNAGC 1 cut(s) 332
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 3 cut(s) 95, 890, 1183
BsaJI CCNNGG 4 cut(s) 445, 516, 762, 1083
BsaWI WCCGGW 2 cut(s) 662, 1561
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
Bse1I ACTGG 3 cut(s) 684, 1339, 1574
Bse3DI GCAATG 3 cut(s) 198, 719, 1668
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 4 cut(s) 445, 516, 762, 1083
BseGI GGATG 3 cut(s) 8, 101, 768
BseLI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
BseMI GCAATG 3 cut(s) 198, 719, 1668
BseMII CTCAG 3 cut(s) 346, 1443, 1629
BseNI ACTGG 3 cut(s) 684, 1339, 1574
BseRI GAGGAG 2 cut(s) 998, 1540
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 5 cut(s) 190, 472, 635, 1487, 1627
BseYI CCCAGC 2 cut(s) 170, 1285
BsgI GTGCAG 1 cut(s) 947
BshFI GGCC 5 cut(s) 344, 521, 867, 1164, 1225
BshNI GGYRCC 3 cut(s) 571, 737, 1249
BsiHKAI GWGCWC 1 cut(s) 1334
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 4 cut(s) 195, 345, 663, 1562
BslFI GGGAC 3 cut(s) 441, 1101, 1396
BslI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
BsmAI GTCTC 3 cut(s) 579, 1172, 1300
BsmBI CGTCTC 2 cut(s) 1172, 1300
BsmFI GGGAC 3 cut(s) 441, 1101, 1396
BsmI GAATGC 2 cut(s) 390, 1229
BsnI GGCC 5 cut(s) 344, 521, 867, 1164, 1225
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 3 cut(s) 612, 1068, 1334
Bsp143I GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
Bsp1720I GCTNAGC 1 cut(s) 332
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 3 cut(s) 626, 1247, 1314
BspANI GGCC 5 cut(s) 344, 521, 867, 1164, 1225
BspCNI CTCAG 3 cut(s) 345, 1442, 1628
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 3 cut(s) 371, 700, 751
BspQI GCTCTTC 1 cut(s) 1470
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 3 cut(s) 571, 737, 1249
BsrDI GCAATG 3 cut(s) 198, 719, 1668
BsrI ACTGG 3 cut(s) 684, 1339, 1574
BssECI CCNNGG 4 cut(s) 445, 516, 762, 1083
BssMI GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
BssSI CACGAG 2 cut(s) 1067, 1754
BssT1I CCWWGG 3 cut(s) 516, 762, 1083
Bst2BI CACGAG 2 cut(s) 1067, 1754
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 8 cut(s) 269, 373, 742, 925, 1715, 1728, 1733, 1803
Bst6I CTCTTC 4 cut(s) 128, 595, 903, 1470
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 2 cut(s) 1227, 1516
BstDEI CTNAG 4 cut(s) 332, 1116, 1429, 1615
BstDSI CCRYGG 1 cut(s) 762
BstF5I GGATG 3 cut(s) 8, 101, 768
BstKTI GATC 6 cut(s) 366, 681, 695, 759, 859, 1668
BstMAI GTCTC 3 cut(s) 579, 1172, 1300
BstMBI GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
BstMWI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1256
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 1334
BstV1I GCAGC 5 cut(s) 190, 472, 635, 1487, 1627
BstV2I GAAGAC 2 cut(s) 148, 1572
BstXI CCANNNNNNTGG 2 cut(s) 315, 619
BsuRI GGCC 5 cut(s) 344, 521, 867, 1164, 1225
BtgI CCRYGG 1 cut(s) 762
BtsCI GGATG 3 cut(s) 8, 101, 768
BtsI GCAGTG 3 cut(s) 1362, 1525, 1608
BtsIMutI CAGTG 7 cut(s) 930, 1057, 1332, 1362, 1525, 1608, 1720
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 2 cut(s) 1227, 1516
CaiI CAGNNNCTG 1 cut(s) 1640
CciI TCATGA 1 cut(s) 1170
Cfr13I GGNCC 1 cut(s) 1383
CseI GACGC 1 cut(s) 176
Csp6I GTAC 7 cut(s) 738, 921, 953, 1210, 1311, 1729, 1734
CviAII CATG 7 cut(s) 337, 763, 839, 965, 1171, 1213, 1601
CviQI GTAC 7 cut(s) 738, 921, 953, 1210, 1311, 1729, 1734
DdeI CTNAG 4 cut(s) 332, 1116, 1429, 1615
DpnI GATC 6 cut(s) 365, 680, 694, 758, 858, 1667
DpnII GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
DraIII CACNNNGTG 1 cut(s) 1411
EaeI YGGCCR 2 cut(s) 342, 1162
Eam1104I CTCTTC 4 cut(s) 128, 595, 903, 1470
EarI CTCTTC 4 cut(s) 128, 595, 903, 1470
Eco130I CCWWGG 3 cut(s) 516, 762, 1083
Eco147I AGGCCT 3 cut(s) 521, 867, 1225
Eco24I GRGCYC 2 cut(s) 612, 1068
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1383
Eco57I CTGAAG 1 cut(s) 829
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 3 cut(s) 516, 762, 1083
EcoT38I GRGCYC 2 cut(s) 612, 1068
ErhI CCWWGG 3 cut(s) 516, 762, 1083
Esp3I CGTCTC 2 cut(s) 1172, 1300
FaeI CATG 7 cut(s) 340, 766, 842, 968, 1174, 1216, 1604
FaqI GGGAC 3 cut(s) 441, 1101, 1396
FatI CATG 7 cut(s) 336, 762, 838, 964, 1170, 1212, 1600
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 1 cut(s) 53
FbaI TGATCA 2 cut(s) 678, 1665
Fnu4HI GCNGC 6 cut(s) 204, 486, 624, 1248, 1501, 1641
FokI GGATG 2 cut(s) 88, 755
FriOI GRGCYC 2 cut(s) 612, 1068
Fsp4HI GCNGC 6 cut(s) 204, 486, 624, 1248, 1501, 1641
FspBI CTAG 5 cut(s) 227, 554, 818, 1037, 1235
GluI GCNGC 6 cut(s) 204, 486, 624, 1248, 1501, 1641
GsaI CCCAGC 2 cut(s) 174, 1289
GsuI CTGGAG 2 cut(s) 291, 1356
HaeIII GGCC 5 cut(s) 344, 521, 867, 1164, 1225
HapII CCGG 4 cut(s) 195, 345, 663, 1562
HgaI GACGC 1 cut(s) 176
Hin1II CATG 7 cut(s) 340, 766, 842, 968, 1174, 1216, 1604
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 3 cut(s) 666, 1100, 1707
HpaII CCGG 4 cut(s) 195, 345, 663, 1562
HphI GGTGA 4 cut(s) 401, 492, 673, 959
Hpy166II GTNNAC 3 cut(s) 245, 1332, 1736
Hpy188I TCNGA 3 cut(s) 166, 791, 861
Hpy188III TCNNGA 9 cut(s) 112, 804, 1075, 1104, 1171, 1184, 1200, 1381, 1768
Hpy8I GTNNAC 3 cut(s) 245, 1332, 1736
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 4 cut(s) 97, 224, 801, 1475
HpyCH4III ACNGT 8 cut(s) 269, 373, 742, 925, 1715, 1728, 1733, 1803
HpyCH4IV ACGT 2 cut(s) 998, 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1256
HpyF3I CTNAG 4 cut(s) 332, 1116, 1429, 1615
HpySE526I ACGT 2 cut(s) 998, 1293
Hsp92II CATG 7 cut(s) 340, 766, 842, 968, 1174, 1216, 1604
KpnI GGTACC 1 cut(s) 741
Ksp22I TGATCA 2 cut(s) 678, 1665
Kzo9I GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
LguI GCTCTTC 1 cut(s) 1470
LmnI GCTCC 3 cut(s) 607, 813, 1337
Lsp1109I GCAGC 5 cut(s) 190, 472, 635, 1487, 1627
LweI GCATC 2 cut(s) 57, 1627
MaeI CTAG 5 cut(s) 227, 554, 818, 1037, 1235
MaeII ACGT 2 cut(s) 998, 1293
MaeIII GTNAC 7 cut(s) 286, 440, 649, 994, 1237, 1520, 1691
MalI GATC 6 cut(s) 365, 680, 694, 758, 858, 1667
MboI GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
MfeI CAATTG 4 cut(s) 654, 774, 1059, 1815
MhlI GDGCHC 3 cut(s) 612, 1068, 1334
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 189, 341
MseI TTAA 2 cut(s) 35, 638
MslI CAYNNNNRTG 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 4 cut(s) 195, 345, 663, 1562
MspR9I CCNGG 1 cut(s) 446
MunI CAATTG 4 cut(s) 654, 774, 1059, 1815
Mva1269I GAATGC 2 cut(s) 390, 1229
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1256
NcoI CCATGG 1 cut(s) 762
NdeI CATATG 1 cut(s) 53
NdeII GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
NlaIII CATG 7 cut(s) 340, 766, 842, 968, 1174, 1216, 1604
NlaIV GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
NmuCI GTSAC 4 cut(s) 994, 1237, 1520, 1691
NspV TTCGAA 1 cut(s) 394
PagI TCATGA 1 cut(s) 1170
PceI AGGCCT 3 cut(s) 521, 867, 1225
PciSI GCTCTTC 1 cut(s) 1470
PctI GAATGC 2 cut(s) 390, 1229
PfeI GAWTC 2 cut(s) 666, 1707
PkrI GCNGC 6 cut(s) 205, 487, 625, 1249, 1502, 1642
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PshBI ATTAAT 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 2 cut(s) 170, 1285
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
PspPI GGNCC 1 cut(s) 1383
PsrI GAACNNNNNNTAC 2 cut(s) 1726, 1758
PstNI CAGNNNCTG 1 cut(s) 1640
RsaI GTAC 7 cut(s) 739, 922, 954, 1211, 1312, 1730, 1735
RsaNI GTAC 7 cut(s) 738, 921, 953, 1210, 1311, 1729, 1734
RseI CAYNNNNRTG 1 cut(s) 593
SapI GCTCTTC 1 cut(s) 1470
SaqAI TTAA 2 cut(s) 35, 638
SatI GCNGC 6 cut(s) 204, 486, 624, 1248, 1501, 1641
Sau3AI GATC 6 cut(s) 363, 678, 692, 756, 856, 1665
Sau96I GGNCC 1 cut(s) 1383
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 3 cut(s) 612, 1068, 1334
SfaNI GCATC 2 cut(s) 57, 1627
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 1383
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 3 cut(s) 110, 869, 1198
SmoI CTYRAG 3 cut(s) 110, 869, 1198
SpeI ACTAGT 1 cut(s) 553
SseBI AGGCCT 3 cut(s) 521, 867, 1225
SsiI CCGC 3 cut(s) 626, 1247, 1314
SspI AATATT 1 cut(s) 324
SspMI CTAG 5 cut(s) 227, 554, 818, 1037, 1235
StuI AGGCCT 3 cut(s) 521, 867, 1225
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 3 cut(s) 516, 762, 1083
TaaI ACNGT 8 cut(s) 269, 373, 742, 925, 1715, 1728, 1733, 1803
TaiI ACGT 2 cut(s) 1001, 1296
TaqI TCGA 1 cut(s) 394
TatI WGTACW 3 cut(s) 920, 952, 1209
TauI GCSGC 1 cut(s) 1250
TfiI GAWTC 2 cut(s) 666, 1707
Tru1I TTAA 2 cut(s) 35, 638
Tru9I TTAA 2 cut(s) 35, 638
TscAI CASTG 7 cut(s) 930, 1057, 1339, 1362, 1525, 1615, 1720
TseFI GTSAC 4 cut(s) 994, 1237, 1520, 1691
TseI GCWGC 5 cut(s) 203, 485, 623, 1500, 1640
Tsp45I GTSAC 4 cut(s) 994, 1237, 1520, 1691
TspDTI ATGAA 5 cut(s) 923, 950, 1137, 1503, 1734
TspGWI ACGGA 3 cut(s) 87, 393, 1673
TspRI CASTG 7 cut(s) 930, 1057, 1339, 1362, 1525, 1615, 1720
VneI GTGCAC 1 cut(s) 1330
VpaK11BI GGWCC 1 cut(s) 1383
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 1 cut(s) 1391
XspI CTAG 5 cut(s) 227, 554, 818, 1037, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.