Rroxscaffold_7G00198780

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001224
Physical Location & Seq
Forward (+)
44474814 .. 44477009
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198780.1

Sequence Viewer

Length: 1620 bp
ATGTCTTCATCCTCATCAAAAGCTTTCCAATGGTTAAGCCTTGTGGGCATCATATGGCTTCAATCCATAAACGGAACAAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTCCCTCTCCCAACTACAAATCAACAGTCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTCTGGCTAGTCCTCATGATCGGCTCACTTCTCGGGTTGATTGGCTACGGTGTCCAATTTCTATTCGTAACCAACCATATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACTATGGTGGCCGGAAATAGTGTCTGTTGGATCAACACCGTTTGCTATGTTGTTTGCATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAGGGATTGAGTGCAAAAATCTACACAGATATCGTTGGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCCAAGGCCTATCTTCTTCTAAACGCTCTCCAGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTTGTGAGAGACATTGATGTGGGAAAAGGGGAGCCCAGAAATATGGCAGCGGGGTTCAAATTAATGTTTGTGGTAACAATTGCCACAGGAATCTATGCTGTGATCACCAGTTTAGGATCAATGTCTATCGACTTATCGCCATTGCACAGTATGATAGGTACCGCATTGTTCCTACTGATGCCCTTGGTCGTCCCAATTCTGGAAAAAATCGGAGAAAAATTATCAAGAAGATGGAGGAGCCTAGACAGAGAGGACATAGTGCACAATTTCAGCACAGATGATCAGAAAGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGGAGAAGAAGAGGAACATAGTACAGTGCATGTTCATAATGAGGTTGGTGAAGTACAAATTCGTCATGGGATTGCTGTTAGAGAGGAGATTGGAGTGAGGTTGATGGTGAAGAGGATAGATTTCTGGTTATATTTCCTAGTATATATTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTCGGACAGATAGTAGAGTCAAGAGGCTCAAGTAGGACTTCTTCTCTGGTTTCTTTATCTTCTTCATTCGGGTTCTTTGGCCGTCTTATGACTTCACTTCTGGACTATTTCTTCTCAAGTAAGTATATGATTTCAAGGCCTGCATTCCTAGTGACATTAGCGGCACCAATAGCAGGAGCTTTCTTCTTGCTTCTCAACCCATCTAACGTCTCCCTTATCATCAGTTCCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGCTATTCGGGACCAAGAATTTCTCAGTAAATCACAATGTGGTGGTTGCGAATATCTCAATCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTCTCTATCGGAGGGAAGGAAATGAAGTTGGTAGCTGCTTGGGAATGGATTGTTACAGAAGCACCTTCATTATCTGGGGCAGTCTTTGTACGTTAGGGACTACTCTAGCTTTAGTGCTATATGCAAGAACACGAAAGTTCTACTCCAAAATATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

539

Amino Acids

59.08

Weight (kDa)

8.89

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 10 - 258 4.3e-76 Nodulin-like
NFD4_C PF23262 326 - 536 3.5e-100 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 734
AccB1I GGYRCC 3 cut(s) 571, 734, 1246
AciI CCGC 4 cut(s) 626, 738, 1244, 1311
AclWI GGATC 2 cut(s) 371, 700
AcoI YGGCCR 2 cut(s) 342, 1162
AcsI RAATTY 2 cut(s) 957, 1387
AdeI CACNNNGTG 1 cut(s) 1408
AfaI GTAC 4 cut(s) 736, 922, 954, 1552
AfiI CCNNNNNNNGG 3 cut(s) 171, 775, 1256
AgsI TTSAA 4 cut(s) 62, 634, 1078, 1218
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 444
AleI CACNNNNGTG 1 cut(s) 338
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 5 cut(s) 23, 1262, 1372, 1497, 1571
AluI AGCT 5 cut(s) 23, 1262, 1372, 1497, 1571
Alw21I GWGCWC 2 cut(s) 840, 1331
Alw26I GTCTC 3 cut(s) 155, 579, 1297
Alw44I GTGCAC 2 cut(s) 836, 1327
AlwI GGATC 2 cut(s) 371, 700
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 342, 519, 865, 1162, 1220
ApaLI GTGCAC 2 cut(s) 836, 1327
ApeKI GCWGC 4 cut(s) 203, 485, 623, 1497
ApoI RAATTY 2 cut(s) 957, 1387
ArsI GACNNNNNNTTYG 2 cut(s) 133, 165
AseI ATTAAT 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 1082
Asp718I GGTACC 1 cut(s) 734
AspS9I GGNCC 1 cut(s) 1380
AsuHPI GGTGA 5 cut(s) 401, 492, 673, 959, 1018
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1380
BaeGI GKGCMC 2 cut(s) 840, 1331
BanI GGYRCC 3 cut(s) 571, 734, 1246
BanII GRGCYC 1 cut(s) 612
BarI GAAGNNNNNNTAC 2 cut(s) 1108, 1140
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 2 cut(s) 840, 1331
BbvI GCAGC 4 cut(s) 190, 472, 635, 1484
BccI CCATC 3 cut(s) 801, 997, 1291
BceAI ACGGC 1 cut(s) 1149
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 2 cut(s) 678, 856
BcoDI GTCTC 3 cut(s) 155, 579, 1297
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 6 cut(s) 227, 554, 818, 1037, 1232, 1568
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 5 cut(s) 204, 486, 624, 1245, 1498
BlsI GCNGC 5 cut(s) 205, 487, 625, 1246, 1499
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 1380
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1380
BmiI GGNNCC 6 cut(s) 573, 609, 736, 815, 1248, 1381
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 57, 744
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 3 cut(s) 291, 527, 1353
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 4 cut(s) 95, 890, 1096, 1183
BsaJI CCNNGG 3 cut(s) 445, 516, 759
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 775, 1256
Bse1I ACTGG 2 cut(s) 684, 1336
Bse3DI GCAATG 2 cut(s) 198, 716
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 445, 516, 759
BseGI GGATG 2 cut(s) 8, 101
BseLI CCNNNNNNNGG 3 cut(s) 171, 775, 1256
BseMI GCAATG 2 cut(s) 198, 716
BseMII CTCAG 1 cut(s) 1407
BseNI ACTGG 2 cut(s) 684, 1336
BseRI GAGGAG 2 cut(s) 826, 998
BseSI GKGCMC 2 cut(s) 840, 1331
BseXI GCAGC 4 cut(s) 190, 472, 635, 1484
BshFI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BshNI GGYRCC 3 cut(s) 571, 734, 1246
BsiHKAI GWGCWC 2 cut(s) 840, 1331
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 195, 345
BslFI GGGAC 4 cut(s) 441, 752, 1393, 1573
BslI CCNNNNNNNGG 3 cut(s) 171, 775, 1256
BsmAI GTCTC 3 cut(s) 155, 579, 1297
BsmBI CGTCTC 1 cut(s) 1297
BsmFI GGGAC 4 cut(s) 441, 752, 1393, 1573
BsmI GAATGC 2 cut(s) 390, 1226
BsnI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 3 cut(s) 612, 840, 1331
Bsp143I GATC 5 cut(s) 237, 363, 678, 692, 856
BspACI CCGC 4 cut(s) 626, 738, 1244, 1311
BspANI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BspCNI CTCAG 1 cut(s) 1406
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 6 cut(s) 573, 609, 736, 815, 1248, 1381
BspPI GGATC 2 cut(s) 371, 700
BspQI GCTCTTC 1 cut(s) 1467
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 3 cut(s) 571, 734, 1246
BsrDI GCAATG 2 cut(s) 198, 716
BsrI ACTGG 2 cut(s) 684, 1336
BssECI CCNNGG 3 cut(s) 445, 516, 759
BssMI GATC 5 cut(s) 237, 363, 678, 692, 856
BssT1I CCWWGG 2 cut(s) 516, 759
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 5 cut(s) 149, 269, 373, 725, 925
Bst6I CTCTTC 3 cut(s) 903, 1004, 1467
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 1224
BstDEI CTNAG 1 cut(s) 1393
BstF5I GGATG 2 cut(s) 8, 101
BstKTI GATC 5 cut(s) 240, 366, 681, 695, 859
BstMAI GTCTC 3 cut(s) 155, 579, 1297
BstMBI GATC 5 cut(s) 237, 363, 678, 692, 856
BstMWI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 1 cut(s) 932
BstSCI CCNGG 1 cut(s) 444
BstSLI GKGCMC 2 cut(s) 840, 1331
BstV1I GCAGC 4 cut(s) 190, 472, 635, 1484
BstV2I GAAGAC 1 cut(s) 148
BstXI CCANNNNNNTGG 2 cut(s) 315, 619
BsuRI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BtsCI GGATG 2 cut(s) 8, 101
BtsI GCAGTG 1 cut(s) 1359
BtsIMutI CAGTG 4 cut(s) 930, 1057, 1329, 1359
Cac8I GCNNGC 1 cut(s) 1224
CciI TCATGA 1 cut(s) 234
Cfr13I GGNCC 1 cut(s) 1380
CseI GACGC 1 cut(s) 176
Csp6I GTAC 4 cut(s) 735, 921, 953, 1551
CviAII CATG 3 cut(s) 235, 929, 965
CviQI GTAC 4 cut(s) 735, 921, 953, 1551
DdeI CTNAG 1 cut(s) 1393
DpnI GATC 5 cut(s) 239, 365, 680, 694, 858
DpnII GATC 5 cut(s) 237, 363, 678, 692, 856
DraIII CACNNNGTG 1 cut(s) 1408
EaeI YGGCCR 2 cut(s) 342, 1162
Eam1104I CTCTTC 3 cut(s) 903, 1004, 1467
EarI CTCTTC 3 cut(s) 903, 1004, 1467
EciI GGCGGA 1 cut(s) 1300
Eco130I CCWWGG 2 cut(s) 516, 759
Eco147I AGGCCT 3 cut(s) 521, 867, 1222
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1380
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 516, 759
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 2 cut(s) 516, 759
Esp3I CGTCTC 1 cut(s) 1297
FaeI CATG 3 cut(s) 238, 932, 968
FalI AAGNNNNNCTT 2 cut(s) 1105, 1137
FaqI GGGAC 4 cut(s) 441, 752, 1393, 1573
FatI CATG 3 cut(s) 234, 928, 964
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 1 cut(s) 53
FbaI TGATCA 2 cut(s) 678, 856
Fnu4HI GCNGC 5 cut(s) 204, 486, 624, 1245, 1498
FokI GGATG 1 cut(s) 88
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 5 cut(s) 204, 486, 624, 1245, 1498
FspBI CTAG 6 cut(s) 227, 554, 818, 1037, 1232, 1568
GluI GCNGC 5 cut(s) 204, 486, 624, 1245, 1498
GsuI CTGGAG 3 cut(s) 291, 527, 1353
HaeIII GGCC 5 cut(s) 344, 521, 867, 1164, 1222
HapII CCGG 2 cut(s) 195, 345
HgaI GACGC 1 cut(s) 176
Hin1II CATG 3 cut(s) 238, 932, 968
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 2 cut(s) 666, 1100
HpaII CCGG 2 cut(s) 195, 345
HphI GGTGA 5 cut(s) 401, 492, 673, 959, 1018
Hpy166II GTNNAC 2 cut(s) 838, 1329
Hpy188I TCNGA 5 cut(s) 166, 788, 861, 1088, 1473
Hpy188III TCNNGA 8 cut(s) 112, 235, 776, 801, 1075, 1104, 1184, 1378
Hpy8I GTNNAC 2 cut(s) 838, 1329
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 97, 224, 1093, 1472, 1537
HpyCH4III ACNGT 5 cut(s) 149, 269, 373, 725, 925
HpyCH4IV ACGT 2 cut(s) 1290, 1553
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
HpyF3I CTNAG 1 cut(s) 1393
HpySE526I ACGT 2 cut(s) 1290, 1553
Hsp92II CATG 3 cut(s) 238, 932, 968
KpnI GGTACC 1 cut(s) 738
Ksp22I TGATCA 2 cut(s) 678, 856
Kzo9I GATC 5 cut(s) 237, 363, 678, 692, 856
LguI GCTCTTC 1 cut(s) 1467
LmnI GCTCC 4 cut(s) 607, 813, 1259, 1334
Lsp1109I GCAGC 4 cut(s) 190, 472, 635, 1484
LweI GCATC 2 cut(s) 57, 744
MaeI CTAG 6 cut(s) 227, 554, 818, 1037, 1232, 1568
MaeII ACGT 2 cut(s) 1290, 1553
MaeIII GTNAC 5 cut(s) 286, 440, 649, 1234, 1514
MalI GATC 5 cut(s) 239, 365, 680, 694, 858
MboI GATC 5 cut(s) 237, 363, 678, 692, 856
MfeI CAATTG 2 cut(s) 654, 1059
MhlI GDGCHC 3 cut(s) 612, 840, 1331
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 189, 341
MroXI GAANNNNTTC 1 cut(s) 1082
MseI TTAA 2 cut(s) 35, 638
MslI CAYNNNNRTG 2 cut(s) 338, 593
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 2 cut(s) 195, 345
MspR9I CCNGG 1 cut(s) 446
MunI CAATTG 2 cut(s) 654, 1059
Mva1269I GAATGC 2 cut(s) 390, 1226
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
NdeI CATATG 1 cut(s) 53
NdeII GATC 5 cut(s) 237, 363, 678, 692, 856
NlaIII CATG 3 cut(s) 238, 932, 968
NlaIV GGNNCC 6 cut(s) 573, 609, 736, 815, 1248, 1381
NmuCI GTSAC 1 cut(s) 1234
NspI RCATGY 1 cut(s) 932
NspV TTCGAA 1 cut(s) 394
OliI CACNNNNGTG 1 cut(s) 338
PagI TCATGA 1 cut(s) 234
PceI AGGCCT 3 cut(s) 521, 867, 1222
PciSI GCTCTTC 1 cut(s) 1467
PctI GAATGC 2 cut(s) 390, 1226
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 5 cut(s) 205, 487, 625, 1246, 1499
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PshBI ATTAAT 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 6 cut(s) 573, 609, 736, 815, 1248, 1381
PspPI GGNCC 1 cut(s) 1380
RsaI GTAC 4 cut(s) 736, 922, 954, 1552
RsaNI GTAC 4 cut(s) 735, 921, 953, 1551
RseI CAYNNNNRTG 2 cut(s) 338, 593
SapI GCTCTTC 1 cut(s) 1467
SaqAI TTAA 2 cut(s) 35, 638
SatI GCNGC 5 cut(s) 204, 486, 624, 1245, 1498
Sau3AI GATC 5 cut(s) 237, 363, 678, 692, 856
Sau96I GGNCC 1 cut(s) 1380
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 3 cut(s) 612, 840, 1331
SfaNI GCATC 2 cut(s) 57, 744
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 1380
SmiMI CAYNNNNRTG 2 cut(s) 338, 593
SmlI CTYRAG 4 cut(s) 110, 869, 1111, 1198
SmoI CTYRAG 4 cut(s) 110, 869, 1111, 1198
SpeI ACTAGT 1 cut(s) 553
SseBI AGGCCT 3 cut(s) 521, 867, 1222
SsiI CCGC 4 cut(s) 626, 738, 1244, 1311
SspI AATATT 1 cut(s) 324
SspMI CTAG 6 cut(s) 227, 554, 818, 1037, 1232, 1568
StuI AGGCCT 3 cut(s) 521, 867, 1222
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 2 cut(s) 516, 759
TaaI ACNGT 5 cut(s) 149, 269, 373, 725, 925
TaiI ACGT 2 cut(s) 1293, 1556
TaqI TCGA 2 cut(s) 394, 705
TatI WGTACW 2 cut(s) 920, 952
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 2 cut(s) 35, 638
Tru9I TTAA 2 cut(s) 35, 638
TscAI CASTG 4 cut(s) 930, 1057, 1336, 1359
TseFI GTSAC 1 cut(s) 1234
TseI GCWGC 4 cut(s) 203, 485, 623, 1497
Tsp45I GTSAC 1 cut(s) 1234
TspDTI ATGAA 4 cut(s) 923, 1137, 1500, 1519
TspGWI ACGGA 2 cut(s) 87, 393
TspRI CASTG 4 cut(s) 930, 1057, 1336, 1359
VneI GTGCAC 2 cut(s) 836, 1327
VpaK11BI GGWCC 1 cut(s) 1380
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 2 cut(s) 957, 1387
XceI RCATGY 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 6 cut(s) 227, 554, 818, 1037, 1232, 1568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.