FvH4_3g40010

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001224
Physical Location & Seq
Forward (+)
33856751 .. 33859157
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40010.t1

Sequence Viewer

Length: 1614 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGCTAAGCCTTGTGGGCATCATATGGCTTCAATCTATAAACGGAACAAACACCACCTTCCCTGCGTACTCATCCCAACTCAAGAACACTCTCTCCATGTCCCAACTGCAGATCAACAATCTCGTCTTTGCCTCCGACGCAGGGAAACTATTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATGATCGGCTCTCTTCTCGGGTTAATCGGCTACGGCGTGCAATTTCTCTTCGTAACGAACCAACTCGCATCTCCAGCATATTGGCAAGTATTCTTGCTCACCATGGTGGCCGGAAATAGTGTATGTTGGATCAACACTGTTTGCTATGTTGTCTGTATTCGGAACTTTCCGTTTCACCAACAGGTTGCTGTGGGATTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTTTCTTTTCTTCTTCACCTCATAAGAGAGCCAGGGCTTATCTTCTTCTAAATTCTCTCCTGCCTTTAATAGTCTGTGCTGTGGTGGCACCGTTCGTGAGAGACATTGATGTGGTTACTGGGAAGCAGCCAAGGAACATGGCAACGGGGTTCAGATTAATGTTTGTGATAACCATTGCCACCGGAATCTATGCGGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAATGATAGGTACCGGGGTGTTCCTACTGATCCCCTTGGTAATCCCAATTGCGGAAAAAATCAGAGATATATTATCAAGAAGATGGAGCCTAGCAGACAGAGAGAACAAAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAGAGTGGTGTGGTGAAAGAGGAAGAAGAAGAGGAACATACTACCACAGTGCAGGTTCATAATGAGGTCAGCGAAGTACAGGTTCAGCACGGTTTTGGTGTAAGAGAGGAGATTGGAGTGAAGCTGATGGTGAGAAGGATAGATTTCTGGTTATATTTCCTAGTCTACTTTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAACAGAGTCAAGAGGCTCATCTAAGACTTCTTCCTTGGTTTCTTTATCTTCTTCATTCGGGTTCTTTGGCCGTCTCATGCCTTCTCTTCTCGATTATTTCTTCGCAAGAAGCAAGCATATGATTTCAAGGCCTGCATTCCTGGTGGCAGTAGCGGCACCGATAGCAGGAGTTTTCTTCTTGCTTCTCAACCCGGCTAACGTCTCCCTTATCATCAGTACCGCCGTTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGTTATTCGGGACCAAGAAGTTCTCAATAAATCACAATGTGGTGGTTGCGAATATTTCAATCGGGTCGTTTCTTTTTGGCTACTCGTCTGCTCTTCTTTATCGCAAGGAAGGAAATGATGATGGTAGCTGTTTGGGAATGGCTTGTTACAAAGGCACCTTCATTATCTGGGGTAGTCTTTGTACATTAGGGACTGTTCTAGCTTTAATGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

538

Amino Acids

59.04

Weight (kDa)

9.14

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 254 1.5e-73 Nodulin-like
NFD4_C PF23262 321 - 534 1.7e-103 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 910
Acc65I GGTACC 1 cut(s) 722
AccB1I GGYRCC 4 cut(s) 568, 722, 1240, 1517
AccI GTMKAC 1 cut(s) 1032
AciI CCGC 4 cut(s) 674, 764, 1238, 1305
AclWI GGATC 3 cut(s) 368, 700, 736
AcoI YGGCCR 2 cut(s) 339, 1153
AcsI RAATTY 1 cut(s) 532
AdeI CACNNNGTG 1 cut(s) 1402
AfaI GTAC 6 cut(s) 95, 724, 827, 945, 1303, 1546
AfiI CCNNNNNNNGG 3 cut(s) 168, 763, 1250
AgsI TTSAA 4 cut(s) 59, 1069, 1212, 1422
AjnI CCWGG 2 cut(s) 512, 1224
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 4 cut(s) 20, 991, 1491, 1565
AluI AGCT 4 cut(s) 20, 991, 1491, 1565
Alw21I GWGCWC 1 cut(s) 1325
Alw26I GTCTC 3 cut(s) 576, 1163, 1291
Alw44I GTGCAC 1 cut(s) 1321
AlwI GGATC 3 cut(s) 368, 700, 736
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 339, 853, 1153, 1214
ApaLI GTGCAC 1 cut(s) 1321
ApeKI GCWGC 2 cut(s) 200, 607
ApoI RAATTY 1 cut(s) 532
AseI ATTAAT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 722
AspS9I GGNCC 1 cut(s) 1374
AsuC2I CCSGG 2 cut(s) 727, 1277
AsuHPI GGTGA 6 cut(s) 322, 398, 489, 673, 892, 1009
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 1374
BaeGI GKGCMC 1 cut(s) 1325
BaeI ACNNNNGTAYC 2 cut(s) 714, 747
BanI GGYRCC 4 cut(s) 568, 722, 1240, 1517
Bbv12I GWGCWC 1 cut(s) 1325
BbvI GCAGC 2 cut(s) 187, 619
BccI CCATC 3 cut(s) 789, 988, 1478
BceAI ACGGC 3 cut(s) 280, 1140, 1292
BciT130I CCWGG 2 cut(s) 514, 1226
BclI TGATCA 1 cut(s) 844
BcnI CCSGG 2 cut(s) 727, 1277
BcoDI GTCTC 3 cut(s) 576, 1163, 1291
BfaI CTAG 4 cut(s) 224, 803, 1028, 1562
BfmI CTRYAG 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 910
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 201, 608, 1239
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 3 cut(s) 202, 609, 1240
Bme1390I CCNGG 4 cut(s) 514, 727, 1226, 1277
Bme18I GGWCC 1 cut(s) 1374
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 1374
BmiI GGNNCC 6 cut(s) 570, 724, 800, 1242, 1375, 1519
BmrFI CCNGG 4 cut(s) 514, 727, 1226, 1277
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 54, 308
BmuI ACTGGG 1 cut(s) 609
BpmI CTGGAG 2 cut(s) 288, 1347
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 92, 878
BpuMI CCSGG 2 cut(s) 727, 1277
BsaJI CCNNGG 8 cut(s) 333, 442, 513, 611, 726, 747, 1074, 1119
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 763, 1250
Bse1I ACTGG 3 cut(s) 604, 684, 1330
Bse3DI GCAATG 2 cut(s) 195, 654
BseBI CCWGG 2 cut(s) 514, 1226
BseDI CCNNGG 8 cut(s) 333, 442, 513, 611, 726, 747, 1074, 1119
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 3 cut(s) 168, 763, 1250
BseMI GCAATG 2 cut(s) 195, 654
BseNI ACTGG 3 cut(s) 604, 684, 1330
BseRI GAGGAG 1 cut(s) 989
BseSI GKGCMC 1 cut(s) 1325
BseXI GCAGC 2 cut(s) 187, 619
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 4 cut(s) 341, 855, 1155, 1216
BshNI GGYRCC 4 cut(s) 568, 722, 1240, 1517
BsiHKAI GWGCWC 1 cut(s) 1325
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 5 cut(s) 192, 342, 663, 726, 1277
BslFI GGGAC 4 cut(s) 112, 1092, 1387, 1567
BslI CCNNNNNNNGG 3 cut(s) 168, 763, 1250
BsmAI GTCTC 3 cut(s) 576, 1163, 1291
BsmBI CGTCTC 2 cut(s) 1163, 1291
BsmFI GGGAC 4 cut(s) 112, 1092, 1387, 1567
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 4 cut(s) 341, 855, 1155, 1216
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 1 cut(s) 1325
Bsp1407I TGTACA 2 cut(s) 825, 1544
Bsp143I GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
Bsp1720I GCTNAGC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 674, 764, 1238, 1305
BspANI GGCC 4 cut(s) 341, 855, 1155, 1216
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 6 cut(s) 570, 724, 800, 1242, 1375, 1519
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 3 cut(s) 368, 700, 736
BspQI GCTCTTC 1 cut(s) 1461
BspT107I GGYRCC 4 cut(s) 568, 722, 1240, 1517
BsrDI GCAATG 2 cut(s) 195, 654
BsrGI TGTACA 2 cut(s) 825, 1544
BsrI ACTGG 3 cut(s) 604, 684, 1330
BssECI CCNNGG 8 cut(s) 333, 442, 513, 611, 726, 747, 1074, 1119
BssMI GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
BssT1I CCWWGG 6 cut(s) 333, 442, 611, 747, 1074, 1119
Bst2UI CCWGG 2 cut(s) 514, 1226
Bst4CI ACNGT 5 cut(s) 370, 573, 916, 959, 1558
Bst6I CTCTTC 5 cut(s) 249, 284, 891, 1176, 1461
BstAUI TGTACA 2 cut(s) 825, 1544
BstC8I GCNNGC 3 cut(s) 269, 1199, 1218
BstDEI CTNAG 3 cut(s) 32, 706, 1107
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 8 cut(s) 141, 237, 363, 695, 702, 744, 847, 1610
BstMAI GTCTC 3 cut(s) 576, 1163, 1291
BstMBI GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
BstMWI GCNNNNNNNGC 7 cut(s) 42, 164, 305, 566, 861, 1238, 1247
BstNI CCWGG 2 cut(s) 514, 1226
BstSCI CCNGG 4 cut(s) 512, 725, 1224, 1275
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 1325
BstV1I GCAGC 2 cut(s) 187, 619
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 341, 855, 1155, 1216
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 1353
BtsIMutI CAGTG 6 cut(s) 366, 484, 921, 1048, 1323, 1353
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 3 cut(s) 269, 1199, 1218
CciI TCATGA 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 1374
CseI GACGC 1 cut(s) 173
Csp6I GTAC 6 cut(s) 94, 723, 826, 944, 1302, 1545
CviAII CATG 5 cut(s) 124, 232, 334, 619, 1162
CviQI GTAC 6 cut(s) 94, 723, 826, 944, 1302, 1545
DdeI CTNAG 3 cut(s) 32, 706, 1107
DpnI GATC 8 cut(s) 140, 236, 362, 694, 701, 743, 846, 1609
DpnII GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
DraIII CACNNNGTG 1 cut(s) 1402
EaeI YGGCCR 2 cut(s) 339, 1153
Eam1104I CTCTTC 5 cut(s) 249, 284, 891, 1176, 1461
EarI CTCTTC 5 cut(s) 249, 284, 891, 1176, 1461
Eco130I CCWWGG 6 cut(s) 333, 442, 611, 747, 1074, 1119
Eco147I AGGCCT 1 cut(s) 1216
Eco47I GGWCC 1 cut(s) 1374
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 512, 1224
EcoT14I CCWWGG 6 cut(s) 333, 442, 611, 747, 1074, 1119
ErhI CCWWGG 6 cut(s) 333, 442, 611, 747, 1074, 1119
Esp3I CGTCTC 2 cut(s) 1163, 1291
FaeI CATG 5 cut(s) 127, 235, 337, 622, 1165
FaqI GGGAC 4 cut(s) 112, 1092, 1387, 1567
FatI CATG 5 cut(s) 123, 231, 333, 618, 1161
FauNDI CATATG 2 cut(s) 50, 1203
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 1 cut(s) 1032
Fnu4HI GCNGC 3 cut(s) 201, 608, 1239
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 3 cut(s) 201, 608, 1239
FspBI CTAG 4 cut(s) 224, 803, 1028, 1562
GluI GCNGC 3 cut(s) 201, 608, 1239
GsuI CTGGAG 2 cut(s) 288, 1347
HaeIII GGCC 4 cut(s) 341, 855, 1155, 1216
HapII CCGG 5 cut(s) 192, 342, 663, 726, 1277
HgaI GACGC 1 cut(s) 173
Hin1II CATG 5 cut(s) 127, 235, 337, 622, 1165
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 666, 1091
HpaII CCGG 5 cut(s) 192, 342, 663, 726, 1277
HphI GGTGA 6 cut(s) 322, 398, 489, 673, 892, 1009
Hpy166II GTNNAC 3 cut(s) 826, 1033, 1323
Hpy188I TCNGA 5 cut(s) 163, 393, 635, 776, 849
Hpy188III TCNNGA 8 cut(s) 109, 232, 577, 789, 1066, 1095, 1175, 1372
Hpy8I GTNNAC 3 cut(s) 826, 1033, 1323
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 6 cut(s) 94, 221, 996, 1176, 1466, 1531
HpyCH4III ACNGT 5 cut(s) 370, 573, 916, 959, 1558
HpyCH4IV ACGT 1 cut(s) 1284
HpyCH4V TGCA 9 cut(s) 136, 271, 455, 919, 1046, 1220, 1323, 1346, 1580
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 164, 305, 566, 861, 1238, 1247
HpyF3I CTNAG 3 cut(s) 32, 706, 1107
HpySE526I ACGT 1 cut(s) 1284
Hsp92II CATG 5 cut(s) 127, 235, 337, 622, 1165
KpnI GGTACC 1 cut(s) 726
Ksp22I TGATCA 1 cut(s) 844
Kzo9I GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
LguI GCTCTTC 1 cut(s) 1461
LmnI GCTCC 2 cut(s) 798, 1328
Lsp1109I GCAGC 2 cut(s) 187, 619
LweI GCATC 2 cut(s) 54, 308
MaeI CTAG 4 cut(s) 224, 803, 1028, 1562
MaeII ACGT 1 cut(s) 1284
MaeIII GTNAC 4 cut(s) 283, 437, 595, 1508
MalI GATC 8 cut(s) 140, 236, 362, 694, 701, 743, 846, 1609
MboI GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
MfeI CAATTG 2 cut(s) 759, 1050
MhlI GDGCHC 1 cut(s) 1325
MluCI AATT 5 cut(s) 272, 532, 759, 1050, 1332
MlyI GAGTC 1 cut(s) 1100
MmeI TCCRAC 2 cut(s) 186, 338
MnlI CCTC 9 cut(s) 19, 169, 239, 510, 880, 892, 925, 967, 1091
MseI TTAA 5 cut(s) 254, 428, 548, 638, 1568
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 5 cut(s) 192, 342, 663, 726, 1277
MspR9I CCNGG 4 cut(s) 514, 727, 1226, 1277
MunI CAATTG 2 cut(s) 759, 1050
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 2 cut(s) 514, 1226
MwoI GCNNNNNNNGC 7 cut(s) 42, 164, 305, 566, 861, 1238, 1247
NciI CCSGG 2 cut(s) 727, 1277
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 2 cut(s) 50, 1203
NdeII GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
NlaIII CATG 5 cut(s) 127, 235, 337, 622, 1165
NlaIV GGNNCC 6 cut(s) 570, 724, 800, 1242, 1375, 1519
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 231
PceI AGGCCT 1 cut(s) 1216
PciSI GCTCTTC 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 1220
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 3 cut(s) 202, 609, 1240
PleI GAGTC 1 cut(s) 1099
PpsI GAGTC 1 cut(s) 1099
PshBI ATTAAT 1 cut(s) 638
Psp6I CCWGG 2 cut(s) 512, 1224
PspGI CCWGG 2 cut(s) 512, 1224
PspN4I GGNNCC 6 cut(s) 570, 724, 800, 1242, 1375, 1519
PspPI GGNCC 1 cut(s) 1374
PsrI GAACNNNNNNTAC 2 cut(s) 809, 841
PstI CTGCAG 1 cut(s) 138
RsaI GTAC 6 cut(s) 95, 724, 827, 945, 1303, 1546
RsaNI GTAC 6 cut(s) 94, 723, 826, 944, 1302, 1545
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1461
SaqAI TTAA 5 cut(s) 254, 428, 548, 638, 1568
SatI GCNGC 3 cut(s) 201, 608, 1239
Sau3AI GATC 8 cut(s) 138, 234, 360, 692, 699, 741, 844, 1607
Sau96I GGNCC 1 cut(s) 1374
SchI GAGTC 1 cut(s) 1100
ScrFI CCNGG 4 cut(s) 514, 727, 1226, 1277
SduI GDGCHC 1 cut(s) 1325
SfaNI GCATC 2 cut(s) 54, 308
SfcI CTRYAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 1374
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 2 cut(s) 107, 857
SmoI CTYRAG 2 cut(s) 107, 857
Sse9I AATT 5 cut(s) 272, 532, 759, 1050, 1332
SseBI AGGCCT 1 cut(s) 1216
SsiI CCGC 4 cut(s) 674, 764, 1238, 1305
SspI AATATT 1 cut(s) 1417
SspMI CTAG 4 cut(s) 224, 803, 1028, 1562
StuI AGGCCT 1 cut(s) 1216
StyD4I CCNGG 4 cut(s) 512, 725, 1224, 1275
StyI CCWWGG 6 cut(s) 333, 442, 611, 747, 1074, 1119
TaaI ACNGT 5 cut(s) 370, 573, 916, 959, 1558
TaiI ACGT 1 cut(s) 1287
TaqI TCGA 1 cut(s) 1176
TasI AATT 5 cut(s) 272, 532, 759, 1050, 1332
TatI WGTACW 3 cut(s) 825, 943, 1544
TauI GCSGC 1 cut(s) 1241
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 5 cut(s) 254, 428, 548, 638, 1568
Tru9I TTAA 5 cut(s) 254, 428, 548, 638, 1568
TscAI CASTG 6 cut(s) 373, 484, 921, 1048, 1330, 1353
TseI GCWGC 2 cut(s) 200, 607
TspDTI ATGAA 3 cut(s) 914, 1128, 1513
TspGWI ACGGA 2 cut(s) 84, 390
TspRI CASTG 6 cut(s) 373, 484, 921, 1048, 1330, 1353
VneI GTGCAC 1 cut(s) 1321
VpaK11BI GGWCC 1 cut(s) 1374
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 1032
XspI CTAG 4 cut(s) 224, 803, 1028, 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.