Rh6CG166900

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001224
Physical Location & Seq
Reverse (-)
22608317 .. 22610101
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG166900.1

Sequence Viewer

Length: 1623 bp
ATGTCTTCATCCTCATCAAAAGCTTTCCAATGGTTAAGCCTTGTGGGCATCATATGGCTTCAATCCATAAACGGAACGAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTTCCTCTCCCAACTACAAATCAACAATCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTCTGGCTAGTCCTCATTATCGGCTCACTTCTCGGGTTGATTGGCTACGGTGTCCAATTTCTATTCGTAACTAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTACTCACCATGGTGGCCGGAAATAGTGTCTGTTGGATCAACACCGTTTGCTATGTTGTTTGCATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAGGGATTGAGTGCAAAAATCTACACAGATATCGTTGGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCCAAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTTGTGAGAGACATCGATGTGGGAAGAGGGGAGCCCAGAAATATGGCAGCGGGGTTCAAATTAATGTTTGTGGTAACAATTGCCACCGGAATCTATGCCGTGATCACCAGTTTAGGATCAATGTCGTCTATCCACTTATCGCCATTGCAGGGTGTGATAGGTACCGTATTGTTCCTACTGATCCCCATGGTCATCCCAATTGTGGAAAAAATCGGAGAAAAATTATCTAGAAGGTGGAGCCTAGACAGAGAGAACCGAGTGCATGACTTCAGCATAGAAGATCAGAAAGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAAGATAGTACAGTGCAGGTTCATAATGAGGTTGGTGAAGTTCAGGTTCATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGAAAAGGATAGATTTCTGGTTATATTTCCTAGTGTATATTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCATTTGGGTTCTTTGGCCGTCTCATGACTTCACTTCTGGCCTATTTCTTCTCAAGAAGCAAGTACATGATTTCAAGGCCTGCATTCCTAGTGACATTAGCGGCACCAATAGCAGGAGTTTTCTTCTTGCTTCTCAACCCAGCTAACGTCTCCCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGCTATTCGGGACCAAGAATTTCTCAATAAATCACAATGTGGTGGTTGTGAATATCTCAATCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTATTTATCGCAAGGAAGGAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTATAGGAGCACCTTCATTATTTGGGGTAGTCTTTGTACGTTAGGGACTACTCTAGCTTTTGCGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAATATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

540

Amino Acids

59.15

Weight (kDa)

8.88

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 10 - 259 2e-74 Nodulin-like
NFD4_C PF23262 324 - 537 8.5e-100 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 919
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 3 cut(s) 571, 737, 1249
AciI CCGC 3 cut(s) 626, 1247, 1314
AclWI GGATC 3 cut(s) 371, 700, 751
AcoI YGGCCR 2 cut(s) 342, 1162
AcsI RAATTY 1 cut(s) 1390
AcuI CTGAAG 1 cut(s) 829
AdeI CACNNNGTG 1 cut(s) 1411
AfaI GTAC 5 cut(s) 739, 922, 1211, 1312, 1555
AfiI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
AgsI TTSAA 4 cut(s) 62, 634, 1078, 1221
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 444
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 5 cut(s) 23, 1289, 1375, 1500, 1574
AluI AGCT 5 cut(s) 23, 1289, 1375, 1500, 1574
Alw21I GWGCWC 2 cut(s) 1334, 1529
Alw26I GTCTC 3 cut(s) 579, 1172, 1300
Alw44I GTGCAC 1 cut(s) 1330
AlwI GGATC 3 cut(s) 371, 700, 751
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 6 cut(s) 342, 519, 865, 1162, 1185, 1223
ApaLI GTGCAC 1 cut(s) 1330
ApeKI GCWGC 4 cut(s) 203, 485, 623, 1500
ApoI RAATTY 1 cut(s) 1390
AseI ATTAAT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 737
AspLEI GCGC 1 cut(s) 1582
AspS9I GGNCC 1 cut(s) 1383
AsuHPI GGTGA 6 cut(s) 325, 401, 492, 673, 959, 1018
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1383
BaeGI GKGCMC 1 cut(s) 1334
BanI GGYRCC 3 cut(s) 571, 737, 1249
BanII GRGCYC 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 2 cut(s) 1334, 1529
BbvI GCAGC 4 cut(s) 190, 472, 635, 1487
BccI CCATC 2 cut(s) 997, 1487
BceAI ACGGC 2 cut(s) 659, 1149
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 579, 1172, 1300
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 7 cut(s) 227, 554, 804, 818, 1037, 1235, 1571
BfuAI ACCTGC 1 cut(s) 919
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 5 cut(s) 204, 486, 624, 1248, 1501
BlsI GCNGC 5 cut(s) 205, 487, 625, 1249, 1502
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 1383
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1383
BmiI GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 2 cut(s) 291, 1356
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 3 cut(s) 95, 890, 1183
Bsa29I ATCGAT 1 cut(s) 591
BsaJI CCNNGG 5 cut(s) 336, 445, 516, 762, 1083
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
Bse1I ACTGG 2 cut(s) 684, 1339
Bse3DI GCAATG 2 cut(s) 198, 719
BseBI CCWGG 1 cut(s) 446
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 5 cut(s) 336, 445, 516, 762, 1083
BseGI GGATG 3 cut(s) 8, 101, 768
BseLI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
BseMI GCAATG 2 cut(s) 198, 719
BseNI ACTGG 2 cut(s) 684, 1339
BseRI GAGGAG 1 cut(s) 998
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 4 cut(s) 190, 472, 635, 1487
BseYI CCCAGC 1 cut(s) 1285
BsgI GTGCAG 1 cut(s) 947
BshFI GGCC 6 cut(s) 344, 521, 867, 1164, 1187, 1225
BshNI GGYRCC 3 cut(s) 571, 737, 1249
BshVI ATCGAT 1 cut(s) 591
BsiHKAI GWGCWC 2 cut(s) 1334, 1529
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 195, 345, 663
BslFI GGGAC 4 cut(s) 441, 1101, 1396, 1576
BslI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1259
BsmAI GTCTC 3 cut(s) 579, 1172, 1300
BsmBI CGTCTC 2 cut(s) 1172, 1300
BsmFI GGGAC 4 cut(s) 441, 1101, 1396, 1576
BsmI GAATGC 2 cut(s) 390, 1229
BsnI GGCC 6 cut(s) 344, 521, 867, 1164, 1187, 1225
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 3 cut(s) 612, 1334, 1529
Bsp143I GATC 5 cut(s) 363, 678, 692, 756, 856
Bsp19I CCATGG 2 cut(s) 336, 762
BspACI CCGC 3 cut(s) 626, 1247, 1314
BspANI GGCC 6 cut(s) 344, 521, 867, 1164, 1187, 1225
BspDI ATCGAT 1 cut(s) 591
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 3 cut(s) 371, 700, 751
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 3 cut(s) 571, 737, 1249
BsrDI GCAATG 2 cut(s) 198, 719
BsrI ACTGG 2 cut(s) 684, 1339
BssECI CCNNGG 5 cut(s) 336, 445, 516, 762, 1083
BssMI GATC 5 cut(s) 363, 678, 692, 756, 856
BssT1I CCWWGG 4 cut(s) 336, 516, 762, 1083
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 4 cut(s) 269, 373, 742, 925
Bst6I CTCTTC 3 cut(s) 128, 595, 903
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 1227
BstDEI CTNAG 1 cut(s) 1116
BstDSI CCRYGG 2 cut(s) 336, 762
BstF5I GGATG 3 cut(s) 8, 101, 768
BstHHI GCGC 1 cut(s) 1582
BstKTI GATC 5 cut(s) 366, 681, 695, 759, 859
BstMAI GTCTC 3 cut(s) 579, 1172, 1300
BstMBI GATC 5 cut(s) 363, 678, 692, 756, 856
BstMWI GCNNNNNNNGC 5 cut(s) 45, 167, 873, 1256, 1586
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 1334
BstV1I GCAGC 4 cut(s) 190, 472, 635, 1487
BstV2I GAAGAC 1 cut(s) 148
BstXI CCANNNNNNTGG 2 cut(s) 315, 619
Bsu15I ATCGAT 1 cut(s) 591
BsuRI GGCC 6 cut(s) 344, 521, 867, 1164, 1187, 1225
BsuTUI ATCGAT 1 cut(s) 591
BtgI CCRYGG 2 cut(s) 336, 762
BtsCI GGATG 3 cut(s) 8, 101, 768
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 4 cut(s) 930, 1057, 1332, 1362
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 1227
CciI TCATGA 1 cut(s) 1170
CfoI GCGC 1 cut(s) 1582
Cfr13I GGNCC 1 cut(s) 1383
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 1 cut(s) 176
Csp6I GTAC 5 cut(s) 738, 921, 1210, 1311, 1554
CviAII CATG 6 cut(s) 337, 763, 839, 965, 1171, 1213
CviQI GTAC 5 cut(s) 738, 921, 1210, 1311, 1554
DdeI CTNAG 1 cut(s) 1116
DpnI GATC 5 cut(s) 365, 680, 694, 758, 858
DpnII GATC 5 cut(s) 363, 678, 692, 756, 856
DraIII CACNNNGTG 1 cut(s) 1411
EaeI YGGCCR 2 cut(s) 342, 1162
Eam1104I CTCTTC 3 cut(s) 128, 595, 903
EarI CTCTTC 3 cut(s) 128, 595, 903
Eco130I CCWWGG 4 cut(s) 336, 516, 762, 1083
Eco147I AGGCCT 3 cut(s) 521, 867, 1225
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1383
Eco57I CTGAAG 1 cut(s) 829
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 4 cut(s) 336, 516, 762, 1083
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 4 cut(s) 336, 516, 762, 1083
Esp3I CGTCTC 2 cut(s) 1172, 1300
FaeI CATG 6 cut(s) 340, 766, 842, 968, 1174, 1216
FaqI GGGAC 4 cut(s) 441, 1101, 1396, 1576
FatI CATG 6 cut(s) 336, 762, 838, 964, 1170, 1212
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 1 cut(s) 53
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 5 cut(s) 204, 486, 624, 1248, 1501
FokI GGATG 2 cut(s) 88, 755
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 5 cut(s) 204, 486, 624, 1248, 1501
FspBI CTAG 7 cut(s) 227, 554, 804, 818, 1037, 1235, 1571
GlaI GCGC 1 cut(s) 1581
GluI GCNGC 5 cut(s) 204, 486, 624, 1248, 1501
GsaI CCCAGC 1 cut(s) 1289
GsuI CTGGAG 2 cut(s) 291, 1356
HaeIII GGCC 6 cut(s) 344, 521, 867, 1164, 1187, 1225
HapII CCGG 3 cut(s) 195, 345, 663
HgaI GACGC 1 cut(s) 176
HhaI GCGC 1 cut(s) 1582
Hin1II CATG 6 cut(s) 340, 766, 842, 968, 1174, 1216
Hin6I GCGC 1 cut(s) 1580
HinP1I GCGC 1 cut(s) 1580
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 2 cut(s) 666, 1100
HpaII CCGG 3 cut(s) 195, 345, 663
HphI GGTGA 6 cut(s) 325, 401, 492, 673, 959, 1018
Hpy166II GTNNAC 1 cut(s) 1332
Hpy188I TCNGA 3 cut(s) 166, 791, 861
Hpy188III TCNNGA 7 cut(s) 112, 804, 1075, 1104, 1171, 1200, 1381
Hpy8I GTNNAC 1 cut(s) 1332
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 97, 224, 801, 1475, 1540
HpyCH4III ACNGT 4 cut(s) 269, 373, 742, 925
HpyCH4IV ACGT 2 cut(s) 1293, 1556
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 167, 873, 1256, 1586
HpyF3I CTNAG 1 cut(s) 1116
HpySE526I ACGT 2 cut(s) 1293, 1556
Hsp92II CATG 6 cut(s) 340, 766, 842, 968, 1174, 1216
HspAI GCGC 1 cut(s) 1580
KpnI GGTACC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 5 cut(s) 363, 678, 692, 756, 856
LmnI GCTCC 4 cut(s) 607, 813, 1337, 1524
Lsp1109I GCAGC 4 cut(s) 190, 472, 635, 1487
LweI GCATC 1 cut(s) 57
MaeI CTAG 7 cut(s) 227, 554, 804, 818, 1037, 1235, 1571
MaeII ACGT 2 cut(s) 1293, 1556
MaeIII GTNAC 4 cut(s) 286, 440, 649, 1237
MalI GATC 5 cut(s) 365, 680, 694, 758, 858
MboI GATC 5 cut(s) 363, 678, 692, 756, 856
MfeI CAATTG 3 cut(s) 654, 774, 1059
MhlI GDGCHC 3 cut(s) 612, 1334, 1529
MluCI AATT 8 cut(s) 275, 635, 654, 774, 797, 1059, 1341, 1390
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 189, 341
MseI TTAA 2 cut(s) 35, 638
MslI CAYNNNNRTG 2 cut(s) 338, 593
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 3 cut(s) 195, 345, 663
MspR9I CCNGG 1 cut(s) 446
MunI CAATTG 3 cut(s) 654, 774, 1059
Mva1269I GAATGC 2 cut(s) 390, 1229
MvaI CCWGG 1 cut(s) 446
MwoI GCNNNNNNNGC 5 cut(s) 45, 167, 873, 1256, 1586
NcoI CCATGG 2 cut(s) 336, 762
NdeI CATATG 1 cut(s) 53
NdeII GATC 5 cut(s) 363, 678, 692, 756, 856
NlaIII CATG 6 cut(s) 340, 766, 842, 968, 1174, 1216
NlaIV GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
NmuCI GTSAC 1 cut(s) 1237
NspV TTCGAA 1 cut(s) 394
OliI CACNNNNGTG 1 cut(s) 338
PagI TCATGA 1 cut(s) 1170
PceI AGGCCT 3 cut(s) 521, 867, 1225
PctI GAATGC 2 cut(s) 390, 1229
PfeI GAWTC 1 cut(s) 666
PkrI GCNGC 5 cut(s) 205, 487, 625, 1249, 1502
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PshBI ATTAAT 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 1285
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 6 cut(s) 573, 609, 739, 815, 1251, 1384
PspPI GGNCC 1 cut(s) 1383
RsaI GTAC 5 cut(s) 739, 922, 1211, 1312, 1555
RsaNI GTAC 5 cut(s) 738, 921, 1210, 1311, 1554
RseI CAYNNNNRTG 2 cut(s) 338, 593
SaqAI TTAA 2 cut(s) 35, 638
SatI GCNGC 5 cut(s) 204, 486, 624, 1248, 1501
Sau3AI GATC 5 cut(s) 363, 678, 692, 756, 856
Sau96I GGNCC 1 cut(s) 1383
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 3 cut(s) 612, 1334, 1529
SfaNI GCATC 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 1383
SmiMI CAYNNNNRTG 2 cut(s) 338, 593
SmlI CTYRAG 3 cut(s) 110, 869, 1198
SmoI CTYRAG 3 cut(s) 110, 869, 1198
SpeI ACTAGT 1 cut(s) 553
Sse9I AATT 8 cut(s) 275, 635, 654, 774, 797, 1059, 1341, 1390
SseBI AGGCCT 3 cut(s) 521, 867, 1225
SsiI CCGC 3 cut(s) 626, 1247, 1314
SspI AATATT 1 cut(s) 324
SspMI CTAG 7 cut(s) 227, 554, 804, 818, 1037, 1235, 1571
StuI AGGCCT 3 cut(s) 521, 867, 1225
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 4 cut(s) 336, 516, 762, 1083
TaaI ACNGT 4 cut(s) 269, 373, 742, 925
TaiI ACGT 2 cut(s) 1296, 1559
TaqI TCGA 2 cut(s) 394, 591
TasI AATT 8 cut(s) 275, 635, 654, 774, 797, 1059, 1341, 1390
TatI WGTACW 2 cut(s) 920, 1209
TauI GCSGC 1 cut(s) 1250
TfiI GAWTC 1 cut(s) 666
Tru1I TTAA 2 cut(s) 35, 638
Tru9I TTAA 2 cut(s) 35, 638
TscAI CASTG 4 cut(s) 930, 1057, 1339, 1362
TseFI GTSAC 1 cut(s) 1237
TseI GCWGC 4 cut(s) 203, 485, 623, 1500
Tsp45I GTSAC 1 cut(s) 1237
TspDTI ATGAA 5 cut(s) 923, 950, 1137, 1503, 1522
TspGWI ACGGA 2 cut(s) 87, 393
TspRI CASTG 4 cut(s) 930, 1057, 1339, 1362
VneI GTGCAC 1 cut(s) 1330
VpaK11BI GGWCC 1 cut(s) 1383
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 1 cut(s) 1390
XbaI TCTAGA 1 cut(s) 803
XspI CTAG 7 cut(s) 227, 554, 804, 818, 1037, 1235, 1571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.