Nodulin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 737 |
| AccB1I | GGYRCC | 3 cut(s) | 571, 737, 1249 |
| AciI | CCGC | 3 cut(s) | 626, 1247, 1314 |
| AclWI | GGATC | 3 cut(s) | 371, 700, 751 |
| AcoI | YGGCCR | 2 cut(s) | 342, 1162 |
| AcsI | RAATTY | 1 cut(s) | 1390 |
| AcuI | CTGAAG | 1 cut(s) | 829 |
| AfaI | GTAC | 5 cut(s) | 739, 922, 1211, 1312, 1555 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 171, 725, 778, 1259 |
| AgsI | TTSAA | 4 cut(s) | 62, 634, 1078, 1221 |
| AhlI | ACTAGT | 1 cut(s) | 553 |
| AleI | CACNNNNGTG | 1 cut(s) | 338 |
| AluBI | AGCT | 5 cut(s) | 23, 1289, 1375, 1500, 1574 |
| AluI | AGCT | 5 cut(s) | 23, 1289, 1375, 1500, 1574 |
| Alw21I | GWGCWC | 3 cut(s) | 119, 1334, 1529 |
| Alw26I | GTCTC | 3 cut(s) | 579, 1172, 1300 |
| Alw44I | GTGCAC | 1 cut(s) | 1330 |
| AlwI | GGATC | 3 cut(s) | 371, 700, 751 |
| Ama87I | CYCGRG | 1 cut(s) | 251 |
| AoxI | GGCC | 5 cut(s) | 342, 519, 865, 1162, 1223 |
| ApaLI | GTGCAC | 1 cut(s) | 1330 |
| ApeKI | GCWGC | 4 cut(s) | 203, 485, 623, 1500 |
| ApoI | RAATTY | 1 cut(s) | 1390 |
| Asp718I | GGTACC | 1 cut(s) | 737 |
| AspS9I | GGNCC | 1 cut(s) | 1383 |
| AsuHPI | GGTGA | 6 cut(s) | 325, 401, 492, 673, 959, 1018 |
| AsuII | TTCGAA | 1 cut(s) | 394 |
| AvaI | CYCGRG | 1 cut(s) | 251 |
| AvaII | GGWCC | 1 cut(s) | 1383 |
| BaeGI | GKGCMC | 1 cut(s) | 1334 |
| BanI | GGYRCC | 3 cut(s) | 571, 737, 1249 |
| BanII | GRGCYC | 1 cut(s) | 612 |
| BbsI | GAAGAC | 1 cut(s) | 148 |
| Bbv12I | GWGCWC | 3 cut(s) | 119, 1334, 1529 |
| BbvI | GCAGC | 4 cut(s) | 190, 472, 635, 1487 |
| BccI | CCATC | 4 cut(s) | 134, 316, 997, 1487 |
| BceAI | ACGGC | 2 cut(s) | 659, 1149 |
| BclI | TGATCA | 1 cut(s) | 678 |
| BcoDI | GTCTC | 3 cut(s) | 579, 1172, 1300 |
| BcuI | ACTAGT | 1 cut(s) | 553 |
| BfaI | CTAG | 6 cut(s) | 227, 554, 818, 1037, 1235, 1571 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 45 |
| BisI | GCNGC | 5 cut(s) | 204, 486, 624, 1248, 1501 |
| BlpI | GCTNAGC | 1 cut(s) | 35 |
| BlsI | GCNGC | 5 cut(s) | 205, 487, 625, 1249, 1502 |
| Bme18I | GGWCC | 1 cut(s) | 1383 |
| BmeT110I | CYCGRG | 1 cut(s) | 251 |
| BmgT120I | GGNCC | 1 cut(s) | 1383 |
| BmiI | GGNNCC | 6 cut(s) | 573, 609, 739, 815, 1251, 1384 |
| BmsI | GCATC | 1 cut(s) | 57 |
| BpiI | GAAGAC | 1 cut(s) | 148 |
| BpmI | CTGGAG | 1 cut(s) | 1356 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 35 |
| Bpu14I | TTCGAA | 1 cut(s) | 394 |
| BpuEI | CTTGAG | 3 cut(s) | 95, 890, 1183 |
| BsaJI | CCNNGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| BsaWI | WCCGGW | 1 cut(s) | 662 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 171, 725, 778, 1259 |
| Bse1I | ACTGG | 2 cut(s) | 684, 1339 |
| Bse3DI | GCAATG | 2 cut(s) | 198, 719 |
| BseDI | CCNNGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| BseGI | GGATG | 3 cut(s) | 8, 101, 768 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 171, 725, 778, 1259 |
| BseMI | GCAATG | 2 cut(s) | 198, 719 |
| BseNI | ACTGG | 2 cut(s) | 684, 1339 |
| BseRI | GAGGAG | 1 cut(s) | 998 |
| BseSI | GKGCMC | 1 cut(s) | 1334 |
| BseXI | GCAGC | 4 cut(s) | 190, 472, 635, 1487 |
| BseYI | CCCAGC | 1 cut(s) | 1285 |
| BshFI | GGCC | 5 cut(s) | 344, 521, 867, 1164, 1225 |
| BshNI | GGYRCC | 3 cut(s) | 571, 737, 1249 |
| BsiHKAI | GWGCWC | 3 cut(s) | 119, 1334, 1529 |
| BsiHKCI | CYCGRG | 1 cut(s) | 251 |
| BsiSI | CCGG | 3 cut(s) | 195, 345, 663 |
| BslFI | GGGAC | 4 cut(s) | 441, 1101, 1396, 1576 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 171, 725, 778, 1259 |
| BsmAI | GTCTC | 3 cut(s) | 579, 1172, 1300 |
| BsmBI | CGTCTC | 2 cut(s) | 1172, 1300 |
| BsmFI | GGGAC | 4 cut(s) | 441, 1101, 1396, 1576 |
| BsmI | GAATGC | 1 cut(s) | 390 |
| BsnI | GGCC | 5 cut(s) | 344, 521, 867, 1164, 1225 |
| BsoBI | CYCGRG | 1 cut(s) | 251 |
| Bsp119I | TTCGAA | 1 cut(s) | 394 |
| Bsp1286I | GDGCHC | 4 cut(s) | 119, 612, 1334, 1529 |
| Bsp143I | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 35 |
| Bsp19I | CCATGG | 1 cut(s) | 336 |
| BspACI | CCGC | 3 cut(s) | 626, 1247, 1314 |
| BspANI | GGCC | 5 cut(s) | 344, 521, 867, 1164, 1225 |
| BspHI | TCATGA | 2 cut(s) | 234, 1170 |
| BspLI | GGNNCC | 6 cut(s) | 573, 609, 739, 815, 1251, 1384 |
| BspPI | GGATC | 3 cut(s) | 371, 700, 751 |
| BspT104I | TTCGAA | 1 cut(s) | 394 |
| BspT107I | GGYRCC | 3 cut(s) | 571, 737, 1249 |
| BsrDI | GCAATG | 2 cut(s) | 198, 719 |
| BsrI | ACTGG | 2 cut(s) | 684, 1339 |
| BssECI | CCNNGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| BssMI | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| BssT1I | CCWWGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| Bst4CI | ACNGT | 4 cut(s) | 269, 373, 742, 925 |
| Bst6I | CTCTTC | 1 cut(s) | 903 |
| BstBI | TTCGAA | 1 cut(s) | 394 |
| BstC8I | GCNNGC | 1 cut(s) | 1227 |
| BstDEI | CTNAG | 2 cut(s) | 35, 1116 |
| BstDSI | CCRYGG | 1 cut(s) | 336 |
| BstF5I | GGATG | 3 cut(s) | 8, 101, 768 |
| BstKTI | GATC | 6 cut(s) | 240, 366, 681, 695, 759, 859 |
| BstMAI | GTCTC | 3 cut(s) | 579, 1172, 1300 |
| BstMBI | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 45, 167, 873, 1256 |
| BstNSI | RCATGY | 1 cut(s) | 932 |
| BstSLI | GKGCMC | 1 cut(s) | 1334 |
| BstV1I | GCAGC | 4 cut(s) | 190, 472, 635, 1487 |
| BstV2I | GAAGAC | 1 cut(s) | 148 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 315, 619 |
| BsuRI | GGCC | 5 cut(s) | 344, 521, 867, 1164, 1225 |
| BtgI | CCRYGG | 1 cut(s) | 336 |
| BtsCI | GGATG | 3 cut(s) | 8, 101, 768 |
| BtsI | GCAGTG | 1 cut(s) | 1362 |
| BtsIMutI | CAGTG | 4 cut(s) | 930, 1057, 1332, 1362 |
| Cac8I | GCNNGC | 1 cut(s) | 1227 |
| CciI | TCATGA | 2 cut(s) | 234, 1170 |
| Cfr13I | GGNCC | 1 cut(s) | 1383 |
| CseI | GACGC | 1 cut(s) | 176 |
| Csp6I | GTAC | 5 cut(s) | 738, 921, 1210, 1311, 1554 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 627, 662 |
| CviAII | CATG | 7 cut(s) | 235, 337, 839, 929, 965, 1171, 1213 |
| CviQI | GTAC | 5 cut(s) | 738, 921, 1210, 1311, 1554 |
| DdeI | CTNAG | 2 cut(s) | 35, 1116 |
| DpnI | GATC | 6 cut(s) | 239, 365, 680, 694, 758, 858 |
| DpnII | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| EaeI | YGGCCR | 2 cut(s) | 342, 1162 |
| Eam1104I | CTCTTC | 1 cut(s) | 903 |
| EarI | CTCTTC | 1 cut(s) | 903 |
| Eco130I | CCWWGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| Eco147I | AGGCCT | 3 cut(s) | 521, 867, 1225 |
| Eco24I | GRGCYC | 1 cut(s) | 612 |
| Eco47I | GGWCC | 1 cut(s) | 1383 |
| Eco57I | CTGAAG | 1 cut(s) | 829 |
| Eco88I | CYCGRG | 1 cut(s) | 251 |
| EcoT14I | CCWWGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| EcoT38I | GRGCYC | 1 cut(s) | 612 |
| ErhI | CCWWGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| Esp3I | CGTCTC | 2 cut(s) | 1172, 1300 |
| FaeI | CATG | 7 cut(s) | 238, 340, 842, 932, 968, 1174, 1216 |
| FaqI | GGGAC | 4 cut(s) | 441, 1101, 1396, 1576 |
| FatI | CATG | 7 cut(s) | 234, 336, 838, 928, 964, 1170, 1212 |
| FauI | CCCGC | 1 cut(s) | 619 |
| FauNDI | CATATG | 1 cut(s) | 53 |
| FbaI | TGATCA | 1 cut(s) | 678 |
| Fnu4HI | GCNGC | 5 cut(s) | 204, 486, 624, 1248, 1501 |
| FokI | GGATG | 2 cut(s) | 88, 755 |
| FriOI | GRGCYC | 1 cut(s) | 612 |
| Fsp4HI | GCNGC | 5 cut(s) | 204, 486, 624, 1248, 1501 |
| FspBI | CTAG | 6 cut(s) | 227, 554, 818, 1037, 1235, 1571 |
| GluI | GCNGC | 5 cut(s) | 204, 486, 624, 1248, 1501 |
| GsaI | CCCAGC | 1 cut(s) | 1289 |
| GsuI | CTGGAG | 1 cut(s) | 1356 |
| HaeIII | GGCC | 5 cut(s) | 344, 521, 867, 1164, 1225 |
| HapII | CCGG | 3 cut(s) | 195, 345, 663 |
| HgaI | GACGC | 1 cut(s) | 176 |
| Hin1II | CATG | 7 cut(s) | 238, 340, 842, 932, 968, 1174, 1216 |
| HindIII | AAGCTT | 1 cut(s) | 21 |
| HinfI | GANTC | 2 cut(s) | 666, 1100 |
| HpaII | CCGG | 3 cut(s) | 195, 345, 663 |
| HphI | GGTGA | 6 cut(s) | 325, 401, 492, 673, 959, 1018 |
| Hpy166II | GTNNAC | 1 cut(s) | 1332 |
| Hpy188I | TCNGA | 4 cut(s) | 166, 791, 861, 1407 |
| Hpy188III | TCNNGA | 9 cut(s) | 112, 235, 804, 1075, 1104, 1171, 1184, 1200, 1381 |
| Hpy8I | GTNNAC | 1 cut(s) | 1332 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 5 cut(s) | 97, 224, 801, 1475, 1540 |
| HpyCH4III | ACNGT | 4 cut(s) | 269, 373, 742, 925 |
| HpyCH4IV | ACGT | 2 cut(s) | 1293, 1556 |
| HpyCH4V | TGCA | 9 cut(s) | 390, 458, 724, 838, 928, 1055, 1332, 1355, 1589 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 45, 167, 873, 1256 |
| HpyF3I | CTNAG | 2 cut(s) | 35, 1116 |
| HpySE526I | ACGT | 2 cut(s) | 1293, 1556 |
| Hsp92II | CATG | 7 cut(s) | 238, 340, 842, 932, 968, 1174, 1216 |
| KpnI | GGTACC | 1 cut(s) | 741 |
| Ksp22I | TGATCA | 1 cut(s) | 678 |
| Kzo9I | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| LmnI | GCTCC | 4 cut(s) | 607, 813, 1337, 1524 |
| Lsp1109I | GCAGC | 4 cut(s) | 190, 472, 635, 1487 |
| LweI | GCATC | 1 cut(s) | 57 |
| MaeI | CTAG | 6 cut(s) | 227, 554, 818, 1037, 1235, 1571 |
| MaeII | ACGT | 2 cut(s) | 1293, 1556 |
| MaeIII | GTNAC | 4 cut(s) | 286, 440, 649, 1237 |
| MalI | GATC | 6 cut(s) | 239, 365, 680, 694, 758, 858 |
| MboI | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| MfeI | CAATTG | 3 cut(s) | 654, 774, 1059 |
| MhlI | GDGCHC | 4 cut(s) | 119, 612, 1334, 1529 |
| MluCI | AATT | 6 cut(s) | 275, 654, 774, 1059, 1341, 1390 |
| MlyI | GAGTC | 1 cut(s) | 1109 |
| MmeI | TCCRAC | 2 cut(s) | 189, 341 |
| MslI | CAYNNNNRTG | 2 cut(s) | 338, 593 |
| MspA1I | CMGCKG | 1 cut(s) | 626 |
| MspI | CCGG | 3 cut(s) | 195, 345, 663 |
| MunI | CAATTG | 3 cut(s) | 654, 774, 1059 |
| Mva1269I | GAATGC | 1 cut(s) | 390 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 45, 167, 873, 1256 |
| NcoI | CCATGG | 1 cut(s) | 336 |
| NdeI | CATATG | 1 cut(s) | 53 |
| NdeII | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| NlaIII | CATG | 7 cut(s) | 238, 340, 842, 932, 968, 1174, 1216 |
| NlaIV | GGNNCC | 6 cut(s) | 573, 609, 739, 815, 1251, 1384 |
| NmuCI | GTSAC | 1 cut(s) | 1237 |
| NspI | RCATGY | 1 cut(s) | 932 |
| NspV | TTCGAA | 1 cut(s) | 394 |
| OliI | CACNNNNGTG | 1 cut(s) | 338 |
| PagI | TCATGA | 2 cut(s) | 234, 1170 |
| PceI | AGGCCT | 3 cut(s) | 521, 867, 1225 |
| PctI | GAATGC | 1 cut(s) | 390 |
| PfeI | GAWTC | 1 cut(s) | 666 |
| PkrI | GCNGC | 5 cut(s) | 205, 487, 625, 1249, 1502 |
| PleI | GAGTC | 1 cut(s) | 1108 |
| PpsI | GAGTC | 1 cut(s) | 1108 |
| PspFI | CCCAGC | 1 cut(s) | 1285 |
| PspN4I | GGNNCC | 6 cut(s) | 573, 609, 739, 815, 1251, 1384 |
| PspPI | GGNCC | 1 cut(s) | 1383 |
| RsaI | GTAC | 5 cut(s) | 739, 922, 1211, 1312, 1555 |
| RsaNI | GTAC | 5 cut(s) | 738, 921, 1210, 1311, 1554 |
| RseI | CAYNNNNRTG | 2 cut(s) | 338, 593 |
| SatI | GCNGC | 5 cut(s) | 204, 486, 624, 1248, 1501 |
| Sau3AI | GATC | 6 cut(s) | 237, 363, 678, 692, 756, 856 |
| Sau96I | GGNCC | 1 cut(s) | 1383 |
| SchI | GAGTC | 1 cut(s) | 1109 |
| SduI | GDGCHC | 4 cut(s) | 119, 612, 1334, 1529 |
| SfaNI | GCATC | 1 cut(s) | 57 |
| SfuI | TTCGAA | 1 cut(s) | 394 |
| SinI | GGWCC | 1 cut(s) | 1383 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 338, 593 |
| SmlI | CTYRAG | 3 cut(s) | 110, 869, 1198 |
| SmoI | CTYRAG | 3 cut(s) | 110, 869, 1198 |
| SpeI | ACTAGT | 1 cut(s) | 553 |
| Sse9I | AATT | 6 cut(s) | 275, 654, 774, 1059, 1341, 1390 |
| SseBI | AGGCCT | 3 cut(s) | 521, 867, 1225 |
| SsiI | CCGC | 3 cut(s) | 626, 1247, 1314 |
| SspI | AATATT | 1 cut(s) | 324 |
| SspMI | CTAG | 6 cut(s) | 227, 554, 818, 1037, 1235, 1571 |
| StuI | AGGCCT | 3 cut(s) | 521, 867, 1225 |
| StyI | CCWWGG | 5 cut(s) | 336, 445, 516, 762, 1083 |
| TaaI | ACNGT | 4 cut(s) | 269, 373, 742, 925 |
| TaiI | ACGT | 2 cut(s) | 1296, 1559 |
| TaqI | TCGA | 1 cut(s) | 394 |
| TasI | AATT | 6 cut(s) | 275, 654, 774, 1059, 1341, 1390 |
| TatI | WGTACW | 2 cut(s) | 920, 1209 |
| TauI | GCSGC | 1 cut(s) | 1250 |
| TfiI | GAWTC | 1 cut(s) | 666 |
| TscAI | CASTG | 4 cut(s) | 930, 1057, 1339, 1362 |
| TseFI | GTSAC | 1 cut(s) | 1237 |
| TseI | GCWGC | 4 cut(s) | 203, 485, 623, 1500 |
| Tsp45I | GTSAC | 1 cut(s) | 1237 |
| TspDTI | ATGAA | 5 cut(s) | 923, 950, 1137, 1503, 1522 |
| TspGWI | ACGGA | 2 cut(s) | 87, 393 |
| TspRI | CASTG | 4 cut(s) | 930, 1057, 1339, 1362 |
| VneI | GTGCAC | 1 cut(s) | 1330 |
| VpaK11BI | GGWCC | 1 cut(s) | 1383 |
| XapI | RAATTY | 1 cut(s) | 1390 |
| XceI | RCATGY | 1 cut(s) | 932 |
| XspI | CTAG | 6 cut(s) | 227, 554, 818, 1037, 1235, 1571 |