RchiOBHm_Chr5g0072091

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001224
Physical Location & Seq
Forward (+)
77895149 .. 77895844
696 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34708

Sequence Viewer

Length: 696 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGGCATCATATGGCTTCAATCCATAAATGGAACAAACACAACCTTCCCTGCTTACTCATCCCAACTCAAGAACAGTCTCTCCATGTCCCAAATACAAATCAACAATCTTGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCTGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTTGTAATCGGATCACTCCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAAACCTAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATTAACACCGTATGCTATGTTGTCAGTATTCGAAACTTTCCGCTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGACATTGTCAGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCACATGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGCTGTAGTGGCACCACTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCAAGAAACATGGCAACGGGGTTCAATTTAATATTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

231

Amino Acids

24.98

Weight (kDa)

9.24

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 228 2.4e-70 Nodulin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AciI CCGC 1 cut(s) 401
AclWI GGATC 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 59, 631
AhlI ACTAGT 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 248
ApeKI GCWGC 2 cut(s) 200, 482
AsuHPI GGTGA 5 cut(s) 322, 398, 489, 589, 670
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
BanI GGYRCC 1 cut(s) 568
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 2 cut(s) 187, 469
BceAI ACGGC 2 cut(s) 280, 656
BcoDI GTCTC 1 cut(s) 122
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 3 cut(s) 38, 224, 551
BfmI CTRYAG 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 2 cut(s) 201, 483
BlsI GCNGC 2 cut(s) 202, 484
BmeT110I CYCGRG 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 570, 606
BmsI GCATC 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 247, 333, 442
BsaWI WCCGGW 1 cut(s) 659
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 579, 681
Bse3DI GCAATG 2 cut(s) 195, 651
BseDI CCNNGG 3 cut(s) 247, 333, 442
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 2 cut(s) 579, 681
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 187, 469
BshNI GGYRCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 2 cut(s) 112, 438
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 2 cut(s) 112, 438
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 1 cut(s) 239
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 1 cut(s) 401
BspLI GGNNCC 2 cut(s) 570, 606
BspPI GGATC 1 cut(s) 247
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 1 cut(s) 568
BsrDI GCAATG 2 cut(s) 195, 651
BsrI ACTGG 2 cut(s) 579, 681
BssECI CCNNGG 3 cut(s) 247, 333, 442
BssMI GATC 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 333, 442
Bst4CI ACNGT 2 cut(s) 116, 370
BstACI GRCGYC 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 1 cut(s) 343
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 1 cut(s) 239
BstMWI GCNNNNNNNGC 3 cut(s) 42, 164, 566
BstNSI RCATGY 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 561
BstV1I GCAGC 2 cut(s) 187, 469
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 484, 572
Cac8I GCNNGC 1 cut(s) 343
CseI GACGC 3 cut(s) 173, 256, 340
CviAII CATG 4 cut(s) 124, 334, 515, 616
CviJI RGCY 6 cut(s) 20, 36, 55, 223, 261, 607
CviKI_1 RGCY 6 cut(s) 20, 36, 55, 223, 261, 607
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
Eco130I CCWWGG 2 cut(s) 333, 442
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 333, 442
ErhI CCWWGG 2 cut(s) 333, 442
FaeI CATG 4 cut(s) 127, 337, 518, 619
FaqI GGGAC 2 cut(s) 112, 438
FatI CATG 4 cut(s) 123, 333, 514, 615
FauNDI CATATG 1 cut(s) 50
Fnu4HI GCNGC 2 cut(s) 201, 483
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 2 cut(s) 201, 483
FspBI CTAG 3 cut(s) 38, 224, 551
GluI GCNGC 2 cut(s) 201, 483
GsuI CTGGAG 1 cut(s) 288
HapII CCGG 1 cut(s) 660
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 4 cut(s) 127, 337, 518, 619
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 1 cut(s) 663
HpaII CCGG 1 cut(s) 660
HphI GGTGA 5 cut(s) 322, 398, 489, 589, 670
Hpy188I TCNGA 2 cut(s) 163, 239
Hpy188III TCNNGA 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 94, 221
HpyCH4III ACNGT 2 cut(s) 116, 370
HpyCH4V TGCA 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 164, 566
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 4 cut(s) 127, 337, 518, 619
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 2 cut(s) 187, 469
LweI GCATC 1 cut(s) 54
MaeI CTAG 3 cut(s) 38, 224, 551
MaeIII GTNAC 2 cut(s) 283, 437
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 5 cut(s) 145, 483, 486, 515, 518
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 2 cut(s) 272, 631
MmeI TCCRAC 2 cut(s) 186, 338
MnlI CCTC 4 cut(s) 19, 169, 257, 510
MseI TTAA 3 cut(s) 32, 363, 635
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 1 cut(s) 660
MwoI GCNNNNNNNGC 3 cut(s) 42, 164, 566
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 1 cut(s) 239
NlaIII CATG 4 cut(s) 127, 337, 518, 619
NlaIV GGNNCC 2 cut(s) 570, 606
NspI RCATGY 1 cut(s) 518
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PfeI GAWTC 1 cut(s) 663
PflFI GACNNNGTC 1 cut(s) 473
PkrI GCNGC 2 cut(s) 202, 484
PspN4I GGNNCC 2 cut(s) 570, 606
PsyI GACNNNGTC 1 cut(s) 473
RseI CAYNNNNRTG 2 cut(s) 335, 590
SaqAI TTAA 3 cut(s) 32, 363, 635
SatI GCNGC 2 cut(s) 201, 483
Sau3AI GATC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 514
SetI ASST 7 cut(s) 22, 86, 213, 294, 417, 502, 691
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 2 cut(s) 272, 631
SsiI CCGC 1 cut(s) 401
SspI AATATT 2 cut(s) 321, 639
SspMI CTAG 3 cut(s) 38, 224, 551
StyI CCWWGG 2 cut(s) 333, 442
TaaI ACNGT 2 cut(s) 116, 370
TaqI TCGA 1 cut(s) 391
TasI AATT 2 cut(s) 272, 631
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 3 cut(s) 32, 363, 635
Tru9I TTAA 3 cut(s) 32, 363, 635
TscAI CASTG 2 cut(s) 484, 579
TseI GCWGC 2 cut(s) 200, 482
TspRI CASTG 2 cut(s) 484, 579
Tth111I GACNNNGTC 1 cut(s) 473
XceI RCATGY 1 cut(s) 518
XspI CTAG 3 cut(s) 38, 224, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.