RchiOBHm_Chr5g0062631

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001224
Physical Location & Seq
Reverse (-)
68329613 .. 68331063
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33885

Sequence Viewer

Length: 1308 bp
ATGTACAGACAAACCAATCTTGGCTCCTACTTTGCCATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGCCATCATATGGCTTCAGTCCATAAATGGAACAAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTATCCTTGTCCCAACTACAAATCAACAATCTCGTCTTTGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATAATCGGATCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATCAATACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGCTTCTCCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAGGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTGCCTTTTCTTCTTCACCTCATAAGAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGCTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCGAGAAACATGGCAGCGGGGTTCAATTTGATGTTTGTGATGACCATTGCCACCGGAATCTATGCCGTCATCGCCAGTTTAGGATCAATGATCGGCTTAGACAGAATGATAGGTACCGGAGTGTTCCTACTGATCCCCTTGGTAATCCCAATTGTAGTAAAAATCAGAGAAAAATTATCAAGAAAATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAAAGTGGTGTAGTTAAACAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATGATGAGGTTGGTGAGGTACAGGTACATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGCAAAGGATAGATTTCTGGTTACATTTCCTAGTGTATTTTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCGGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTCCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGACTATTTCTTCTCAAGCAAGTATATGATTTCAAGGCCTGCATTCCTAATGGCATTAGCAGCACCAATGGCAGGAGTTTTTTTTGTTGCTTCTCAACCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

435

Amino Acids

47.78

Weight (kDa)

8.32

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 21 - 268 5e-71 Nodulin-like
NFD4_C PF23262 330 - 430 5.8e-41 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 937
Acc65I GGTACC 1 cut(s) 755
AccB1I GGYRCC 2 cut(s) 604, 755
AccB7I CCANNNNNTGG 1 cut(s) 87
AciI CCGC 3 cut(s) 437, 659, 1115
AclWI GGATC 4 cut(s) 283, 404, 733, 769
AcoI YGGCCR 2 cut(s) 78, 1180
AcuI CTGAAG 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 6 cut(s) 5, 757, 857, 940, 972, 978
AfiI CCNNNNNNNGG 3 cut(s) 87, 204, 1274
AgsI TTSAA 3 cut(s) 667, 1096, 1236
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 1 cut(s) 477
AleI CACNNNNGTG 1 cut(s) 371
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AluBI AGCT 2 cut(s) 56, 1305
AluI AGCT 2 cut(s) 56, 1305
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 1 cut(s) 1190
AlwI GGATC 4 cut(s) 283, 404, 733, 769
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 5 cut(s) 78, 552, 883, 1180, 1238
ApeKI GCWGC 4 cut(s) 236, 518, 656, 1262
Asp718I GGTACC 1 cut(s) 755
AsuC2I CCSGG 1 cut(s) 1171
AsuHPI GGTGA 4 cut(s) 358, 525, 625, 977
AsuII TTCGAA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BalI TGGCCA 1 cut(s) 80
BanI GGYRCC 2 cut(s) 604, 755
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 4 cut(s) 223, 505, 668, 1274
BccI CCATC 2 cut(s) 89, 1015
BceAI ACGGC 3 cut(s) 316, 692, 1167
BciT130I CCWGG 1 cut(s) 479
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 1 cut(s) 1171
BcoDI GTCTC 1 cut(s) 1190
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 5 cut(s) 74, 260, 587, 836, 1055
BfmI CTRYAG 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 937
BisI GCNGC 4 cut(s) 237, 519, 657, 1263
BlsI GCNGC 4 cut(s) 238, 520, 658, 1264
Bme1390I CCNGG 2 cut(s) 479, 1171
BmeT110I CYCGRG 1 cut(s) 284
BmiI GGNNCC 5 cut(s) 25, 606, 642, 757, 833
BmrFI CCNGG 2 cut(s) 479, 1171
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 427
BpuEI CTTGAG 3 cut(s) 128, 908, 1201
BpuMI CCSGG 1 cut(s) 1171
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 4 cut(s) 369, 478, 780, 1101
BsaWI WCCGGW 2 cut(s) 695, 758
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 204, 1274
Bse1I ACTGG 1 cut(s) 717
Bse3DI GCAATG 2 cut(s) 231, 687
BseBI CCWGG 1 cut(s) 479
BseDI CCNNGG 4 cut(s) 369, 478, 780, 1101
BseGI GGATG 1 cut(s) 134
BseLI CCNNNNNNNGG 3 cut(s) 87, 204, 1274
BseMI GCAATG 2 cut(s) 231, 687
BseNI ACTGG 1 cut(s) 717
BseRI GAGGAG 1 cut(s) 1016
BseXI GCAGC 4 cut(s) 223, 505, 668, 1274
BseYI CCCAGC 1 cut(s) 1301
BsgI GTGCAG 1 cut(s) 965
BshFI GGCC 5 cut(s) 80, 554, 885, 1182, 1240
BshNI GGYRCC 2 cut(s) 604, 755
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 4 cut(s) 228, 696, 759, 1170
BslFI GGGAC 3 cut(s) 148, 474, 1119
BslI CCNNNNNNNGG 3 cut(s) 87, 204, 1274
BsmAI GTCTC 1 cut(s) 1190
BsmBI CGTCTC 1 cut(s) 1190
BsmFI GGGAC 3 cut(s) 148, 474, 1119
BsmI GAATGC 1 cut(s) 1244
BsnI GGCC 5 cut(s) 80, 554, 885, 1182, 1240
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 550
Bsp1407I TGTACA 2 cut(s) 3, 855
Bsp143I GATC 6 cut(s) 275, 396, 725, 732, 774, 874
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 3 cut(s) 437, 659, 1115
BspANI GGCC 5 cut(s) 80, 554, 885, 1182, 1240
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 5 cut(s) 25, 606, 642, 757, 833
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 4 cut(s) 283, 404, 733, 769
BspT104I TTCGAA 1 cut(s) 427
BspT107I GGYRCC 2 cut(s) 604, 755
BsrDI GCAATG 2 cut(s) 231, 687
BsrGI TGTACA 2 cut(s) 3, 855
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 4 cut(s) 369, 478, 780, 1101
BssMI GATC 6 cut(s) 275, 396, 725, 732, 774, 874
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 369, 780, 1101
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 2 cut(s) 406, 943
Bst6I CTCTTC 1 cut(s) 921
BstACI GRCGYC 1 cut(s) 303
BstAUI TGTACA 2 cut(s) 3, 855
BstBI TTCGAA 1 cut(s) 427
BstC8I GCNNGC 2 cut(s) 379, 1242
BstDEI CTNAG 2 cut(s) 739, 1134
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 134
BstKTI GATC 6 cut(s) 278, 399, 728, 735, 777, 877
BstMAI GTCTC 1 cut(s) 1190
BstMBI GATC 6 cut(s) 275, 396, 725, 732, 774, 874
BstMWI GCNNNNNNNGC 6 cut(s) 200, 602, 713, 891, 1262, 1271
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 477, 1169
BstSFI CTRYAG 1 cut(s) 597
BstV1I GCAGC 4 cut(s) 223, 505, 668, 1274
BstV2I GAAGAC 1 cut(s) 33
BstXI CCANNNNNNTGG 1 cut(s) 348
BsuRI GGCC 5 cut(s) 80, 554, 885, 1182, 1240
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 697
BtsCI GGATG 1 cut(s) 134
BtsIMutI CAGTG 3 cut(s) 520, 948, 1075
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 2 cut(s) 379, 1242
CciI TCATGA 1 cut(s) 952
CseI GACGC 3 cut(s) 209, 292, 376
Csp6I GTAC 6 cut(s) 4, 756, 856, 939, 971, 977
CviAII CATG 6 cut(s) 37, 370, 652, 953, 983, 1189
CviQI GTAC 6 cut(s) 4, 756, 856, 939, 971, 977
DdeI CTNAG 2 cut(s) 739, 1134
DpnI GATC 6 cut(s) 277, 398, 727, 734, 776, 876
DpnII GATC 6 cut(s) 275, 396, 725, 732, 774, 874
EaeI YGGCCR 2 cut(s) 78, 1180
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
Eco130I CCWWGG 3 cut(s) 369, 780, 1101
Eco147I AGGCCT 2 cut(s) 554, 1240
Eco57I CTGAAG 1 cut(s) 77
Eco88I CYCGRG 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 3 cut(s) 369, 780, 1101
ErhI CCWWGG 3 cut(s) 369, 780, 1101
Esp3I CGTCTC 1 cut(s) 1190
FaeI CATG 6 cut(s) 40, 373, 655, 956, 986, 1192
FaqI GGGAC 3 cut(s) 148, 474, 1119
FatI CATG 6 cut(s) 36, 369, 651, 952, 982, 1188
FauI CCCGC 1 cut(s) 652
FauNDI CATATG 1 cut(s) 86
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 4 cut(s) 237, 519, 657, 1263
FokI GGATG 1 cut(s) 121
Fsp4HI GCNGC 4 cut(s) 237, 519, 657, 1263
FspBI CTAG 5 cut(s) 74, 260, 587, 836, 1055
GluI GCNGC 4 cut(s) 237, 519, 657, 1263
GsaI CCCAGC 1 cut(s) 1305
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 5 cut(s) 80, 554, 885, 1182, 1240
HapII CCGG 4 cut(s) 228, 696, 759, 1170
HgaI GACGC 3 cut(s) 209, 292, 376
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 6 cut(s) 40, 373, 655, 956, 986, 1192
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 2 cut(s) 699, 1118
HpaII CCGG 4 cut(s) 228, 696, 759, 1170
HphI GGTGA 4 cut(s) 358, 525, 625, 977
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 4 cut(s) 199, 275, 809, 879
Hpy188III TCNNGA 6 cut(s) 145, 822, 953, 1093, 1122, 1202
Hpy8I GTNNAC 1 cut(s) 856
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 130, 257, 1203
HpyCH4III ACNGT 2 cut(s) 406, 943
HpyCH4V TGCA 5 cut(s) 491, 946, 1027, 1073, 1244
HpyF10VI GCNNNNNNNGC 6 cut(s) 200, 602, 713, 891, 1262, 1271
HpyF3I CTNAG 2 cut(s) 739, 1134
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 6 cut(s) 40, 373, 655, 956, 986, 1192
KpnI GGTACC 1 cut(s) 759
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 6 cut(s) 275, 396, 725, 732, 774, 874
LmnI GCTCC 3 cut(s) 29, 640, 831
Lsp1109I GCAGC 4 cut(s) 223, 505, 668, 1274
MaeI CTAG 5 cut(s) 74, 260, 587, 836, 1055
MaeIII GTNAC 3 cut(s) 319, 473, 1044
MalI GATC 6 cut(s) 277, 398, 727, 734, 776, 876
MboI GATC 6 cut(s) 275, 396, 725, 732, 774, 874
MfeI CAATTG 2 cut(s) 792, 1077
MhlI GDGCHC 1 cut(s) 550
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 5 cut(s) 308, 667, 792, 815, 1077
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 1 cut(s) 1127
MmeI TCCRAC 3 cut(s) 222, 374, 469
Mox20I TGGCCA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 80
MseI TTAA 2 cut(s) 68, 912
MslI CAYNNNNRTG 2 cut(s) 371, 626
Msp20I TGGCCA 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 4 cut(s) 228, 696, 759, 1170
MspR9I CCNGG 2 cut(s) 479, 1171
MunI CAATTG 2 cut(s) 792, 1077
Mva1269I GAATGC 1 cut(s) 1244
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 6 cut(s) 200, 602, 713, 891, 1262, 1271
NciI CCSGG 1 cut(s) 1171
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 86
NdeII GATC 6 cut(s) 275, 396, 725, 732, 774, 874
NlaIII CATG 6 cut(s) 40, 373, 655, 956, 986, 1192
NlaIV GGNNCC 5 cut(s) 25, 606, 642, 757, 833
NmeAIII GCCGAG 1 cut(s) 669
NspV TTCGAA 1 cut(s) 427
OliI CACNNNNGTG 1 cut(s) 371
PagI TCATGA 1 cut(s) 952
PceI AGGCCT 2 cut(s) 554, 1240
PctI GAATGC 1 cut(s) 1244
PfeI GAWTC 1 cut(s) 699
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 4 cut(s) 238, 520, 658, 1264
PleI GAGTC 1 cut(s) 1126
PpsI GAGTC 1 cut(s) 1126
Psp6I CCWGG 1 cut(s) 477
PspFI CCCAGC 1 cut(s) 1301
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 5 cut(s) 25, 606, 642, 757, 833
PsrI GAACNNNNNNTAC 2 cut(s) 839, 871
RsaI GTAC 6 cut(s) 5, 757, 857, 940, 972, 978
RsaNI GTAC 6 cut(s) 4, 756, 856, 939, 971, 977
RseI CAYNNNNRTG 2 cut(s) 371, 626
SaqAI TTAA 2 cut(s) 68, 912
SatI GCNGC 4 cut(s) 237, 519, 657, 1263
Sau3AI GATC 6 cut(s) 275, 396, 725, 732, 774, 874
SchI GAGTC 1 cut(s) 1127
ScrFI CCNGG 2 cut(s) 479, 1171
SduI GDGCHC 1 cut(s) 550
SfcI CTRYAG 1 cut(s) 597
SfuI TTCGAA 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 371, 626
SmlI CTYRAG 3 cut(s) 143, 887, 1216
SmoI CTYRAG 3 cut(s) 143, 887, 1216
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 5 cut(s) 308, 667, 792, 815, 1077
SseBI AGGCCT 2 cut(s) 554, 1240
SsiI CCGC 3 cut(s) 437, 659, 1115
SspI AATATT 1 cut(s) 357
SspMI CTAG 5 cut(s) 74, 260, 587, 836, 1055
StuI AGGCCT 2 cut(s) 554, 1240
StyD4I CCNGG 2 cut(s) 477, 1169
StyI CCWWGG 3 cut(s) 369, 780, 1101
TaaI ACNGT 2 cut(s) 406, 943
TaqI TCGA 1 cut(s) 427
TasI AATT 5 cut(s) 308, 667, 792, 815, 1077
TatI WGTACW 3 cut(s) 3, 855, 938
TfiI GAWTC 1 cut(s) 699
Tru1I TTAA 2 cut(s) 68, 912
Tru9I TTAA 2 cut(s) 68, 912
TscAI CASTG 3 cut(s) 520, 948, 1075
TseI GCWGC 4 cut(s) 236, 518, 656, 1262
TspDTI ATGAA 1 cut(s) 941
TspRI CASTG 3 cut(s) 520, 948, 1075
Van91I CCANNNNNTGG 1 cut(s) 87
XspI CTAG 5 cut(s) 74, 260, 587, 836, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.