Rroxscaffold_7G00198800

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001224
Physical Location & Seq
Forward (+)
44484725 .. 44487109
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198800.1

Sequence Viewer

Length: 1827 bp
ATGTCTTCATCCTCATCAAAAGCTTTCCAATGGTTAAGCCTTGTGGGCATCATATGGCTTCAATCCATAAACGGAACGAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTCTTCCTCTCCCAACTACAAATCAACAATCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTCTGGCTAGTCCTCATTATCGGCTCACTTCTCGGGTTGATTGGCTACGGTGTCCAATTTCTATTCGTAACTAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACTATGGTGGCCGGAAATAGTGTCTGTTGGATCAACACCGTTTGCTATGTTGTTTGCATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAGGGATTGAGTGCAAAAATCTACACAGATATCGTTGGTGCTGCTTTTTCTTCTTCACCTCATAAGAGAGCCAAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTTGTGAGAGACATTGATGTGGGAACAGGGGAGCCCAGAAATATGGCAGCGGGGTTCAAATTAATGTTTGTGGTAACAATTGCCACCGGAATCTATGCCGTGATCACCAGTTTAGGATCAATGTCGTCTATCCACTTATCGCCATTGCAGGGTGTGATAGGTACCGTATTGTTCCTACTGATCCCCTTGGTCATCCCAATTGTGGAAAAAATCGGAGAAAAATTATCAAGAAGGTGGTGCCTAGACAGAGAGAACAGAGTGCATGATTTCAGCATAGAAGATCAGAAAGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAAAGTAGTACAGTGCAGGTTCATAATGAGGTTGGTGAAGTTCAGGTTCATCATGGGATTGATGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGAAAAGGATAGATTTCTGGTTATATTTCCTAGTGTATATTTTCAGTGCAACACTTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCGGAGTCAAGAGGCTCATTTAAAACTTCTTCTCTGGTTTCTTTATCCTCTTCATTTGGGTTCTTTGGCCGTCTCATGACTTCACTTCTGGAGTATTTCTTCTCAAGCAAGTACATGATTTCAAGGCCTGCATTCCTAGTGACATTAGCGGCACCAATAGCAGGAGTTTTCTTCTTGCTTCTCAACCCAGCTAACGTCTCCCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGCTATTCGGGACCAAGAATTTCTCGATAAATCACAATGTGGTGGTTGTGAATATCTCAATCGGGTCATTTCTTTTTGGCTACTCATCTGCTCTTATTTATCGCAAGGAAGGAAATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTATAGGAGCACCTTCATTATTTGGGGTAGTCTTTACTCCAAGCTACAGCATATGTCTATTATGATGGTTTCAAAAGAGCCCAAAATCTACTCCTCTGCTGAACCGGAAGACTGGTGTGGGAAAGCCGACCAAACATCTGCCCATGTCCTCACTGCCTCAGCTATATCCTTGACAACAGATGCTGCCTCTCACATCAGCACGGGTTTGATCCTTGCTGTATCTTTCGCATATTGTCACAGAAGATGTGGAATCAACAGTGATTGCAAACGGTACACCAATTTCATCAGTCCGCGTGTATCGTTTCCCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

608

Amino Acids

66.62

Weight (kDa)

7.55

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 10 - 259 1.8e-74 Nodulin-like
NFD4_C PF23262 326 - 527 1.6e-90 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 919
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 4 cut(s) 571, 737, 813, 1246
AccII CGCG 1 cut(s) 1807
AciI CCGC 5 cut(s) 626, 1097, 1244, 1311, 1805
AclWI GGATC 4 cut(s) 371, 700, 751, 1717
AcoI YGGCCR 2 cut(s) 342, 1162
AcsI RAATTY 1 cut(s) 1387
AdeI CACNNNGTG 1 cut(s) 1408
AfaI GTAC 5 cut(s) 739, 922, 1208, 1309, 1787
AfiI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1256
AgsI TTSAA 5 cut(s) 62, 634, 1078, 1218, 1587
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 444
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 6 cut(s) 23, 1286, 1372, 1497, 1558, 1676
AluI AGCT 6 cut(s) 23, 1286, 1372, 1497, 1558, 1676
Alw21I GWGCWC 2 cut(s) 1331, 1526
Alw26I GTCTC 3 cut(s) 579, 1172, 1297
Alw44I GTGCAC 1 cut(s) 1327
AlwI GGATC 4 cut(s) 371, 700, 751, 1717
AlwNI CAGNNNCTG 1 cut(s) 1697
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 342, 519, 865, 1162, 1220
ApaLI GTGCAC 1 cut(s) 1327
ApeKI GCWGC 5 cut(s) 203, 485, 623, 1497, 1697
ApoI RAATTY 1 cut(s) 1387
AseI ATTAAT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 1380
AsuHPI GGTGA 5 cut(s) 401, 492, 673, 959, 1018
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 1380
BaeGI GKGCMC 1 cut(s) 1331
BanI GGYRCC 4 cut(s) 571, 737, 813, 1246
BanII GRGCYC 2 cut(s) 612, 1596
BbsI GAAGAC 2 cut(s) 148, 1629
Bbv12I GWGCWC 2 cut(s) 1331, 1526
BbvCI CCTCAGC 1 cut(s) 1672
BbvI GCAGC 5 cut(s) 190, 472, 635, 1484, 1684
BccI CCATC 3 cut(s) 997, 1484, 1573
BceAI ACGGC 2 cut(s) 659, 1149
BciT130I CCWGG 1 cut(s) 446
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 579, 1172, 1297
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 5 cut(s) 227, 554, 818, 1037, 1232
BfmI CTRYAG 1 cut(s) 1559
BfuAI ACCTGC 1 cut(s) 919
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 6 cut(s) 204, 486, 624, 1245, 1498, 1698
BlsI GCNGC 6 cut(s) 205, 487, 625, 1246, 1499, 1699
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 1 cut(s) 1380
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 1380
BmiI GGNNCC 6 cut(s) 573, 609, 739, 815, 1248, 1381
BmrFI CCNGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 57, 1684
BpiI GAAGAC 2 cut(s) 148, 1629
BpmI CTGGAG 3 cut(s) 291, 1205, 1353
Bpu10I CCTNAGC 1 cut(s) 1672
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 3 cut(s) 95, 890, 1183
BsaJI CCNNGG 4 cut(s) 445, 516, 762, 1083
BsaWI WCCGGW 2 cut(s) 662, 1618
Bsc4I CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1256
Bse1I ACTGG 3 cut(s) 684, 1336, 1631
Bse3DI GCAATG 2 cut(s) 198, 719
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 4 cut(s) 445, 516, 762, 1083
BseGI GGATG 3 cut(s) 8, 101, 768
BseLI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1256
BseMI GCAATG 2 cut(s) 198, 719
BseMII CTCAG 1 cut(s) 1686
BseNI ACTGG 3 cut(s) 684, 1336, 1631
BseRI GAGGAG 2 cut(s) 998, 1597
BseSI GKGCMC 1 cut(s) 1331
BseXI GCAGC 5 cut(s) 190, 472, 635, 1484, 1684
BseYI CCCAGC 1 cut(s) 1282
BsgI GTGCAG 1 cut(s) 947
Bsh1236I CGCG 1 cut(s) 1807
BshFI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BshNI GGYRCC 4 cut(s) 571, 737, 813, 1246
BsiHKAI GWGCWC 2 cut(s) 1331, 1526
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 4 cut(s) 195, 345, 663, 1619
BslFI GGGAC 3 cut(s) 441, 1101, 1393
BslI CCNNNNNNNGG 4 cut(s) 171, 725, 778, 1256
BsmAI GTCTC 3 cut(s) 579, 1172, 1297
BsmBI CGTCTC 2 cut(s) 1172, 1297
BsmFI GGGAC 3 cut(s) 441, 1101, 1393
BsmI GAATGC 2 cut(s) 390, 1226
BsnI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 4 cut(s) 612, 1331, 1526, 1596
Bsp143I GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
BspACI CCGC 5 cut(s) 626, 1097, 1244, 1311, 1805
BspANI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BspCNI CTCAG 1 cut(s) 1685
BspFNI CGCG 1 cut(s) 1807
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 6 cut(s) 573, 609, 739, 815, 1248, 1381
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 4 cut(s) 371, 700, 751, 1717
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 4 cut(s) 571, 737, 813, 1246
BsrDI GCAATG 2 cut(s) 198, 719
BsrI ACTGG 3 cut(s) 684, 1336, 1631
BssECI CCNNGG 4 cut(s) 445, 516, 762, 1083
BssMI GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
BssT1I CCWWGG 3 cut(s) 516, 762, 1083
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 6 cut(s) 269, 373, 742, 925, 1772, 1785
Bst6I CTCTTC 3 cut(s) 128, 903, 1150
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 1224
BstDEI CTNAG 1 cut(s) 1672
BstF5I GGATG 3 cut(s) 8, 101, 768
BstFNI CGCG 1 cut(s) 1807
BstKTI GATC 6 cut(s) 366, 681, 695, 759, 859, 1725
BstMAI GTCTC 3 cut(s) 579, 1172, 1297
BstMBI GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
BstMWI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
BstNI CCWGG 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 1 cut(s) 1559
BstSLI GKGCMC 1 cut(s) 1331
BstUI CGCG 1 cut(s) 1807
BstV1I GCAGC 5 cut(s) 190, 472, 635, 1484, 1684
BstV2I GAAGAC 2 cut(s) 148, 1629
BstXI CCANNNNNNTGG 2 cut(s) 315, 619
BsuRI GGCC 5 cut(s) 344, 521, 867, 1164, 1222
BtsCI GGATG 3 cut(s) 8, 101, 768
BtsI GCAGTG 2 cut(s) 1359, 1665
BtsIMutI CAGTG 6 cut(s) 930, 1057, 1329, 1359, 1665, 1777
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 1 cut(s) 1224
CaiI CAGNNNCTG 1 cut(s) 1697
CciI TCATGA 1 cut(s) 1170
Cfr13I GGNCC 1 cut(s) 1380
CseI GACGC 1 cut(s) 176
Csp6I GTAC 5 cut(s) 738, 921, 1207, 1308, 1786
CviAII CATG 5 cut(s) 839, 965, 1171, 1210, 1658
CviQI GTAC 5 cut(s) 738, 921, 1207, 1308, 1786
DdeI CTNAG 1 cut(s) 1672
DpnI GATC 6 cut(s) 365, 680, 694, 758, 858, 1724
DpnII GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
DraI TTTAAA 1 cut(s) 1117
DraIII CACNNNGTG 1 cut(s) 1408
EaeI YGGCCR 2 cut(s) 342, 1162
Eam1104I CTCTTC 3 cut(s) 128, 903, 1150
EarI CTCTTC 3 cut(s) 128, 903, 1150
Eco130I CCWWGG 3 cut(s) 516, 762, 1083
Eco147I AGGCCT 3 cut(s) 521, 867, 1222
Eco24I GRGCYC 2 cut(s) 612, 1596
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1380
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 3 cut(s) 516, 762, 1083
EcoT38I GRGCYC 2 cut(s) 612, 1596
ErhI CCWWGG 3 cut(s) 516, 762, 1083
Esp3I CGTCTC 2 cut(s) 1172, 1297
FaeI CATG 5 cut(s) 842, 968, 1174, 1213, 1661
FaqI GGGAC 3 cut(s) 441, 1101, 1393
FatI CATG 5 cut(s) 838, 964, 1170, 1209, 1657
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 2 cut(s) 53, 1566
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 6 cut(s) 204, 486, 624, 1245, 1498, 1698
FokI GGATG 2 cut(s) 88, 755
FriOI GRGCYC 2 cut(s) 612, 1596
Fsp4HI GCNGC 6 cut(s) 204, 486, 624, 1245, 1498, 1698
FspBI CTAG 5 cut(s) 227, 554, 818, 1037, 1232
GluI GCNGC 6 cut(s) 204, 486, 624, 1245, 1498, 1698
GsaI CCCAGC 1 cut(s) 1286
GsuI CTGGAG 3 cut(s) 291, 1205, 1353
HaeIII GGCC 5 cut(s) 344, 521, 867, 1164, 1222
HapII CCGG 4 cut(s) 195, 345, 663, 1619
HgaI GACGC 1 cut(s) 176
Hin1II CATG 5 cut(s) 842, 968, 1174, 1213, 1661
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 3 cut(s) 666, 1100, 1764
HpaII CCGG 4 cut(s) 195, 345, 663, 1619
HphI GGTGA 5 cut(s) 401, 492, 673, 959, 1018
Hpy166II GTNNAC 2 cut(s) 1329, 1788
Hpy188I TCNGA 3 cut(s) 166, 791, 861
Hpy188III TCNNGA 9 cut(s) 112, 804, 1075, 1104, 1171, 1184, 1378, 1393, 1820
Hpy8I GTNNAC 2 cut(s) 1329, 1788
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 97, 224, 801, 1472, 1537
HpyCH4III ACNGT 6 cut(s) 269, 373, 742, 925, 1772, 1785
HpyCH4IV ACGT 1 cut(s) 1290
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
HpyF3I CTNAG 1 cut(s) 1672
HpySE526I ACGT 1 cut(s) 1290
Hsp92II CATG 5 cut(s) 842, 968, 1174, 1213, 1661
KpnI GGTACC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
LmnI GCTCC 3 cut(s) 607, 1334, 1521
Lsp1109I GCAGC 5 cut(s) 190, 472, 635, 1484, 1684
LweI GCATC 2 cut(s) 57, 1684
MaeI CTAG 5 cut(s) 227, 554, 818, 1037, 1232
MaeII ACGT 1 cut(s) 1290
MaeIII GTNAC 5 cut(s) 286, 440, 649, 1234, 1748
MalI GATC 6 cut(s) 365, 680, 694, 758, 858, 1724
MboI GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
MfeI CAATTG 2 cut(s) 654, 774
MhlI GDGCHC 4 cut(s) 612, 1331, 1526, 1596
MluCI AATT 8 cut(s) 275, 635, 654, 774, 797, 1338, 1387, 1792
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 189, 341
MseI TTAA 3 cut(s) 35, 638, 1116
MslI CAYNNNNRTG 2 cut(s) 338, 593
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 4 cut(s) 195, 345, 663, 1619
MspR9I CCNGG 1 cut(s) 446
MunI CAATTG 2 cut(s) 654, 774
Mva1269I GAATGC 2 cut(s) 390, 1226
MvaI CCWGG 1 cut(s) 446
MvnI CGCG 1 cut(s) 1807
MwoI GCNNNNNNNGC 4 cut(s) 45, 167, 873, 1253
NdeI CATATG 2 cut(s) 53, 1566
NdeII GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
NlaIII CATG 5 cut(s) 842, 968, 1174, 1213, 1661
NlaIV GGNNCC 6 cut(s) 573, 609, 739, 815, 1248, 1381
NmuCI GTSAC 2 cut(s) 1234, 1748
NspV TTCGAA 1 cut(s) 394
OliI CACNNNNGTG 1 cut(s) 338
PagI TCATGA 1 cut(s) 1170
PceI AGGCCT 3 cut(s) 521, 867, 1222
PctI GAATGC 2 cut(s) 390, 1226
PfeI GAWTC 2 cut(s) 666, 1764
PkrI GCNGC 6 cut(s) 205, 487, 625, 1246, 1499, 1699
PleI GAGTC 1 cut(s) 1108
PpsI GAGTC 1 cut(s) 1108
PshBI ATTAAT 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 1282
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 6 cut(s) 573, 609, 739, 815, 1248, 1381
PspPI GGNCC 1 cut(s) 1380
PstNI CAGNNNCTG 1 cut(s) 1697
RsaI GTAC 5 cut(s) 739, 922, 1208, 1309, 1787
RsaNI GTAC 5 cut(s) 738, 921, 1207, 1308, 1786
RseI CAYNNNNRTG 2 cut(s) 338, 593
SaqAI TTAA 3 cut(s) 35, 638, 1116
SatI GCNGC 6 cut(s) 204, 486, 624, 1245, 1498, 1698
Sau3AI GATC 6 cut(s) 363, 678, 692, 756, 856, 1722
Sau96I GGNCC 1 cut(s) 1380
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 4 cut(s) 612, 1331, 1526, 1596
SfaNI GCATC 2 cut(s) 57, 1684
SfcI CTRYAG 1 cut(s) 1559
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 1380
SmiMI CAYNNNNRTG 2 cut(s) 338, 593
SmlI CTYRAG 3 cut(s) 110, 869, 1198
SmoI CTYRAG 3 cut(s) 110, 869, 1198
SpeI ACTAGT 1 cut(s) 553
Sse9I AATT 8 cut(s) 275, 635, 654, 774, 797, 1338, 1387, 1792
SseBI AGGCCT 3 cut(s) 521, 867, 1222
SsiI CCGC 5 cut(s) 626, 1097, 1244, 1311, 1805
SspI AATATT 1 cut(s) 324
SspMI CTAG 5 cut(s) 227, 554, 818, 1037, 1232
StuI AGGCCT 3 cut(s) 521, 867, 1222
StyD4I CCNGG 1 cut(s) 444
StyI CCWWGG 3 cut(s) 516, 762, 1083
TaaI ACNGT 6 cut(s) 269, 373, 742, 925, 1772, 1785
TaiI ACGT 1 cut(s) 1293
TaqI TCGA 2 cut(s) 394, 1394
TasI AATT 8 cut(s) 275, 635, 654, 774, 797, 1338, 1387, 1792
TatI WGTACW 2 cut(s) 920, 1206
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 2 cut(s) 666, 1764
Tru1I TTAA 3 cut(s) 35, 638, 1116
Tru9I TTAA 3 cut(s) 35, 638, 1116
TscAI CASTG 6 cut(s) 930, 1057, 1336, 1359, 1672, 1777
TseFI GTSAC 2 cut(s) 1234, 1748
TseI GCWGC 5 cut(s) 203, 485, 623, 1497, 1697
Tsp45I GTSAC 2 cut(s) 1234, 1748
TspDTI ATGAA 6 cut(s) 923, 950, 1137, 1500, 1519, 1786
TspGWI ACGGA 2 cut(s) 87, 393
TspRI CASTG 6 cut(s) 930, 1057, 1336, 1359, 1672, 1777
VneI GTGCAC 1 cut(s) 1327
VpaK11BI GGWCC 1 cut(s) 1380
VspI ATTAAT 1 cut(s) 638
XapI RAATTY 1 cut(s) 1387
XspI CTAG 5 cut(s) 227, 554, 818, 1037, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.