MD11G1060600.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001224
Physical Location & Seq
Forward (+)
5262275 .. 5265489
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1060600.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGACCCAACTACAGCTCAATAACCTCGCCTTCGCCTCAGATGCCGGAAAACTTCTCGGATGGTTTTCCGGCATTGCTGCCATCCACTTACCCCTCTGGATAGTCCTCATGATCGGTTCGTTTCTGGGGTTAATCGGGTATGGCGTCCAGTTCCTCTTCATCACAAACCTAATCCCATCACCATCCTATTGGCAGATTTTCTTGTTAACTGTTATGGCTGGGAATAGCATTTGCTGGATCAACACTGTGTGCTATGTAGTCAGCATTAGAAACTTCCCATGTCATCAACAAATTGCCGTGGGGATAACAACAAGTTACCAAGGATTGAGCGCCAAGATTTATACAGATATTGTTGGCGCTGCATTTTCATCTTCGCCTCACAGAAGAGCCAAGGCCTACTTACTTCTCAACTCTCTCTTGCCACTTGTTGTGTGTGTTATCGTCGCGCCATTGGTTAGAGACATTGATCTGATGGGAAGGCAAAGTAACATGGAAGCCGGGTTTGTTTTTATGTTCGTCATAACAATTGCTACAGGAATCTACGCGGTGATTAGCAGTTTAGGGTCGCTTTCTAGCGGGTTATCTCCTTTGCATAATGTAATAGGAACCGGATTGTTCCTATTAGTCCCATTAGTAATCCCGCTTGTGGATAAAATGAGAGAAGTATTTTCAAAAAAATGGAACATAAACAGAGAGAGAAGAGTGTATAATTTTACCATAGAGGAGAAGAACAACGAAACGGGGAGCTTAGAGAATGTGGGAGAAGAATTAGATGCTAGACAAGTTCTTGAAGTTGGTGTTAGGGAGGAGATTGGAGTGAAGTTGATGGTGACAAGGATTGATTTTTGGTTATATTTCTTTGTGTATTTGTTCGGTGCAACACTTGGGTTGGTGTTCTTGAACAACTTGGGACAGATTTCAGAGTCCCGGGGCTCGTCCAGAACTTCTTCATTGGTCTCTTTATCCTCTTCATTTGGGTTTTTTGGCCGTCTCATGCCTTCTCTTTTGGACTATTTCTTCTCAAGGAGTAAGTATATGATTTCAAGGCCAGCATTAGTAGTTGCATTAATGGCACCAATAGCAGGAGCCTTCTTCTTGCTTCTCAACCCAGCCAACCTCTCACTTTTCATCAGCACAGCAATTATAGGAGTCTGTACTGGAGCTATTACTTCAATCGCAGTGTCCATAACCACAGAGCTGTTTGGGACCAAGAGTTTCTCAGTGAACCACAATGTGGTGGTGGCCAACATATCAATCGGGTCTTTTGTGTTTGGATACTTGTCTGCTGTTCTTTACCACAAAGAAGGAAATCATGATGGTGACGGGAAGTGCATGGGAATGGCTTGCTACAGAAGCACTTTTATTATATGGGGCTGCCTTTGTTTTCTGGGAACTGGCGTAGCTTTAATGCTCTATGCACGAACACGAAAGTTCTACTCCCAAAGATCATTAAATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

487

Amino Acids

53.42

Weight (kDa)

9.07

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 2 - 215 8.9e-60 Nodulin-like
NFD4_C PF23262 264 - 480 1.2e-104 NFD4, C-terminal domain
MFS_1 PF07690 349 - 476 7.1e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1072
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccII CGCG 2 cut(s) 446, 545
AciI CCGC 3 cut(s) 545, 576, 641
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 2 cut(s) 985, 1242
AcyI GRCGYC 1 cut(s) 144
AdeI CACNNNGTG 2 cut(s) 249, 1234
AfaI GTAC 1 cut(s) 1156
AfiI CCNNNNNNNGG 3 cut(s) 646, 1082, 1234
AgsI TTSAA 5 cut(s) 672, 791, 901, 1044, 1173
AluBI AGCT 5 cut(s) 16, 747, 1163, 1198, 1403
AluI AGCT 5 cut(s) 16, 747, 1163, 1198, 1403
Alw26I GTCTC 3 cut(s) 453, 961, 995
AlwI GGATC 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 927
AoxI GGCC 4 cut(s) 393, 985, 1046, 1242
ApeKI GCWGC 3 cut(s) 77, 359, 1374
AseI ATTAAT 1 cut(s) 1067
Asp700I GAANNNNTTC 1 cut(s) 946
AspLEI GCGC 3 cut(s) 332, 359, 448
AspS9I GGNCC 1 cut(s) 1206
AsuC2I CCSGG 3 cut(s) 499, 928, 929
AsuHPI GGTGA 4 cut(s) 171, 559, 841, 1331
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 1 cut(s) 1206
BalI TGGCCA 1 cut(s) 1244
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 1 cut(s) 935
BbvI GCAGC 3 cut(s) 64, 346, 1361
BccI CCATC 7 cut(s) 54, 89, 184, 190, 466, 820, 1310
BceAI ACGGC 2 cut(s) 281, 972
BciVI GTATCC 1 cut(s) 1268
BcnI CCSGG 3 cut(s) 499, 928, 929
BcoDI GTCTC 3 cut(s) 453, 961, 995
BfaI CTAG 2 cut(s) 573, 777
BfmI CTRYAG 3 cut(s) 11, 531, 1348
BfoI RGCGCY 2 cut(s) 333, 360
BfuI GTATCC 1 cut(s) 1268
BisI GCNGC 3 cut(s) 78, 360, 1375
BlsI GCNGC 3 cut(s) 79, 361, 1376
Bme1390I CCNGG 3 cut(s) 499, 928, 929
Bme18I GGWCC 1 cut(s) 1206
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 1 cut(s) 1206
BmiI GGNNCC 4 cut(s) 607, 1074, 1087, 1207
BmrFI CCNGG 3 cut(s) 499, 928, 929
BmsI GCATC 2 cut(s) 31, 763
BpmI CTGGAG 1 cut(s) 1179
BpuEI CTTGAG 1 cut(s) 1006
BpuMI CCSGG 3 cut(s) 499, 928, 929
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 961
BsaJI CCNNGG 5 cut(s) 297, 319, 390, 927, 928
BsaWI WCCGGW 1 cut(s) 608
Bsc4I CCNNNNNNNGG 3 cut(s) 646, 1082, 1234
Bse1I ACTGG 3 cut(s) 148, 1162, 1399
Bse3DI GCAATG 1 cut(s) 72
BseDI CCNNGG 5 cut(s) 297, 319, 390, 927, 928
BseGI GGATG 3 cut(s) 65, 81, 182
BseLI CCNNNNNNNGG 3 cut(s) 646, 1082, 1234
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 2 cut(s) 51, 1233
BseNI ACTGG 3 cut(s) 148, 1162, 1399
BseRI GAGGAG 2 cut(s) 737, 821
BseXI GCAGC 3 cut(s) 64, 346, 1361
BseYI CCCAGC 2 cut(s) 218, 1108
Bsh1236I CGCG 2 cut(s) 446, 545
BshFI GGCC 4 cut(s) 395, 987, 1048, 1244
BshNI GGYRCC 1 cut(s) 1072
BsiHKCI CYCGRG 1 cut(s) 927
BsiSI CCGG 5 cut(s) 45, 69, 498, 609, 928
BslFI GGGAC 4 cut(s) 611, 910, 924, 1219
BslI CCNNNNNNNGG 3 cut(s) 646, 1082, 1234
BsmAI GTCTC 3 cut(s) 453, 961, 995
BsmBI CGTCTC 1 cut(s) 995
BsmFI GGGAC 4 cut(s) 611, 910, 924, 1219
BsnI GGCC 4 cut(s) 395, 987, 1048, 1244
Bso31I GGTCTC 1 cut(s) 961
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 1 cut(s) 935
Bsp143I GATC 4 cut(s) 111, 237, 466, 1445
BspACI CCGC 3 cut(s) 545, 576, 641
BspANI GGCC 4 cut(s) 395, 987, 1048, 1244
BspCNI CTCAG 2 cut(s) 50, 1232
BspFNI CGCG 2 cut(s) 446, 545
BspHI TCATGA 2 cut(s) 108, 1312
BspLI GGNNCC 4 cut(s) 607, 1074, 1087, 1207
BspPI GGATC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 1072
BspTNI GGTCTC 1 cut(s) 961
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 3 cut(s) 148, 1162, 1399
BssECI CCNNGG 5 cut(s) 297, 319, 390, 927, 928
BssMI GATC 4 cut(s) 111, 237, 466, 1445
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 2 cut(s) 319, 390
Bst4CI ACNGT 2 cut(s) 211, 247
Bst6I CTCTTC 4 cut(s) 161, 379, 694, 973
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 1050, 1345
BstDEI CTNAG 3 cut(s) 37, 748, 1219
BstDSI CCRYGG 1 cut(s) 297
BstF5I GGATG 3 cut(s) 65, 81, 182
BstFNI CGCG 2 cut(s) 446, 545
BstH2I RGCGCY 2 cut(s) 333, 360
BstHHI GCGC 3 cut(s) 332, 359, 448
BstKTI GATC 4 cut(s) 114, 240, 469, 1448
BstMAI GTCTC 3 cut(s) 453, 961, 995
BstMBI GATC 4 cut(s) 111, 237, 466, 1445
BstMWI GCNNNNNNNGC 4 cut(s) 41, 1070, 1079, 1353
BstSCI CCNGG 3 cut(s) 497, 926, 927
BstSFI CTRYAG 3 cut(s) 11, 531, 1348
BstUI CGCG 2 cut(s) 446, 545
BstV1I GCAGC 3 cut(s) 64, 346, 1361
BstXI CCANNNNNNTGG 1 cut(s) 189
BsuI GTATCC 1 cut(s) 1268
BsuRI GGCC 4 cut(s) 395, 987, 1048, 1244
BtgI CCRYGG 1 cut(s) 297
BtsCI GGATG 3 cut(s) 65, 81, 182
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 3 cut(s) 243, 1185, 1227
Cac8I GCNNGC 2 cut(s) 1050, 1345
CciI TCATGA 2 cut(s) 108, 1312
CfoI GCGC 3 cut(s) 332, 359, 448
Cfr13I GGNCC 1 cut(s) 1206
Cfr9I CCCGGG 1 cut(s) 927
CseI GACGC 1 cut(s) 133
Csp6I GTAC 1 cut(s) 1155
CviAII CATG 6 cut(s) 109, 279, 490, 994, 1313, 1333
CviQI GTAC 1 cut(s) 1155
DdeI CTNAG 3 cut(s) 37, 748, 1219
DpnI GATC 4 cut(s) 113, 239, 468, 1447
DpnII GATC 4 cut(s) 111, 237, 466, 1445
DraIII CACNNNGTG 2 cut(s) 249, 1234
EaeI YGGCCR 2 cut(s) 985, 1242
Eam1104I CTCTTC 4 cut(s) 161, 379, 694, 973
EarI CTCTTC 4 cut(s) 161, 379, 694, 973
Eco130I CCWWGG 2 cut(s) 319, 390
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 935
Eco31I GGTCTC 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 1206
Eco88I CYCGRG 1 cut(s) 927
EcoT14I CCWWGG 2 cut(s) 319, 390
EcoT38I GRGCYC 1 cut(s) 935
ErhI CCWWGG 2 cut(s) 319, 390
Esp3I CGTCTC 1 cut(s) 995
FaeI CATG 6 cut(s) 112, 282, 493, 997, 1316, 1336
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 4 cut(s) 611, 910, 924, 1219
FatI CATG 6 cut(s) 108, 278, 489, 993, 1312, 1332
FauI CCCGC 2 cut(s) 569, 648
Fnu4HI GCNGC 3 cut(s) 78, 360, 1375
FokI GGATG 3 cut(s) 68, 72, 169
FriOI GRGCYC 1 cut(s) 935
Fsp4HI GCNGC 3 cut(s) 78, 360, 1375
FspBI CTAG 2 cut(s) 573, 777
GlaI GCGC 3 cut(s) 331, 358, 447
GluI GCNGC 3 cut(s) 78, 360, 1375
GsaI CCCAGC 2 cut(s) 222, 1112
GsuI CTGGAG 1 cut(s) 1179
HaeII RGCGCY 2 cut(s) 333, 360
HaeIII GGCC 4 cut(s) 395, 987, 1048, 1244
HapII CCGG 5 cut(s) 45, 69, 498, 609, 928
HgaI GACGC 1 cut(s) 133
HhaI GCGC 3 cut(s) 332, 359, 448
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 6 cut(s) 112, 282, 493, 997, 1316, 1336
Hin6I GCGC 3 cut(s) 330, 357, 446
HinP1I GCGC 3 cut(s) 330, 357, 446
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HinfI GANTC 3 cut(s) 537, 923, 1149
HpaI GTTAAC 1 cut(s) 207
HpaII CCGG 5 cut(s) 45, 69, 498, 609, 928
HphI GGTGA 4 cut(s) 171, 559, 841, 1331
Hpy166II GTNNAC 2 cut(s) 207, 1225
Hpy188I TCNGA 4 cut(s) 40, 59, 471, 922
Hpy188III TCNNGA 6 cut(s) 97, 109, 788, 898, 939, 1313
Hpy8I GTNNAC 2 cut(s) 207, 1225
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 5 cut(s) 40, 471, 1008, 1099, 1298
HpyCH4III ACNGT 2 cut(s) 211, 247
HpyCH4V TGCA 6 cut(s) 362, 592, 878, 1064, 1332, 1418
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 1070, 1079, 1353
HpyF3I CTNAG 3 cut(s) 37, 748, 1219
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 6 cut(s) 112, 282, 493, 997, 1316, 1336
HspAI GCGC 3 cut(s) 330, 357, 446
KspAI GTTAAC 1 cut(s) 207
Kzo9I GATC 4 cut(s) 111, 237, 466, 1445
LguI GCTCTTC 1 cut(s) 379
LmnI GCTCC 3 cut(s) 744, 1085, 1160
Lsp1109I GCAGC 3 cut(s) 64, 346, 1361
LweI GCATC 2 cut(s) 31, 763
MaeI CTAG 2 cut(s) 573, 777
MaeIII GTNAC 4 cut(s) 314, 485, 829, 1319
MalI GATC 4 cut(s) 113, 239, 468, 1447
MboI GATC 4 cut(s) 111, 237, 466, 1445
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 935
MlsI TGGCCA 1 cut(s) 1244
MluCI AATT 5 cut(s) 291, 525, 709, 767, 1140
MluNI TGGCCA 1 cut(s) 1244
MlyI GAGTC 2 cut(s) 932, 1158
Mox20I TGGCCA 1 cut(s) 1244
MroXI GAANNNNTTC 1 cut(s) 946
MscI TGGCCA 1 cut(s) 1244
MseI TTAA 5 cut(s) 131, 206, 1067, 1406, 1451
MslI CAYNNNNRTG 2 cut(s) 1317, 1337
Msp20I TGGCCA 1 cut(s) 1244
MspI CCGG 5 cut(s) 45, 69, 498, 609, 928
MspR9I CCNGG 3 cut(s) 499, 928, 929
MunI CAATTG 1 cut(s) 525
MvnI CGCG 2 cut(s) 446, 545
MwoI GCNNNNNNNGC 4 cut(s) 41, 1070, 1079, 1353
NciI CCSGG 3 cut(s) 499, 928, 929
NdeII GATC 4 cut(s) 111, 237, 466, 1445
NlaIII CATG 6 cut(s) 112, 282, 493, 997, 1316, 1336
NlaIV GGNNCC 4 cut(s) 607, 1074, 1087, 1207
NmuCI GTSAC 2 cut(s) 829, 1319
PagI TCATGA 2 cut(s) 108, 1312
PceI AGGCCT 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 946
PfeI GAWTC 1 cut(s) 537
PflMI CCANNNNNTGG 1 cut(s) 1234
PkrI GCNGC 3 cut(s) 79, 361, 1376
PleI GAGTC 2 cut(s) 931, 1157
PpsI GAGTC 2 cut(s) 931, 1157
PshBI ATTAAT 1 cut(s) 1067
PspFI CCCAGC 2 cut(s) 218, 1108
PspN4I GGNNCC 4 cut(s) 607, 1074, 1087, 1207
PspPI GGNCC 1 cut(s) 1206
RsaI GTAC 1 cut(s) 1156
RsaNI GTAC 1 cut(s) 1155
RseI CAYNNNNRTG 2 cut(s) 1317, 1337
SapI GCTCTTC 1 cut(s) 379
SaqAI TTAA 5 cut(s) 131, 206, 1067, 1406, 1451
SatI GCNGC 3 cut(s) 78, 360, 1375
Sau3AI GATC 4 cut(s) 111, 237, 466, 1445
Sau96I GGNCC 1 cut(s) 1206
SchI GAGTC 2 cut(s) 932, 1158
ScrFI CCNGG 3 cut(s) 499, 928, 929
SduI GDGCHC 1 cut(s) 935
SetI ASST 8 cut(s) 18, 27, 171, 749, 1119, 1165, 1200, 1405
SfaNI GCATC 2 cut(s) 31, 763
SfcI CTRYAG 3 cut(s) 11, 531, 1348
SinI GGWCC 1 cut(s) 1206
SmaI CCCGGG 1 cut(s) 929
SmiMI CAYNNNNRTG 2 cut(s) 1317, 1337
SmlI CTYRAG 1 cut(s) 1021
SmoI CTYRAG 1 cut(s) 1021
Sse9I AATT 5 cut(s) 291, 525, 709, 767, 1140
SseBI AGGCCT 1 cut(s) 395
SsiI CCGC 3 cut(s) 545, 576, 641
SspMI CTAG 2 cut(s) 573, 777
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 3 cut(s) 497, 926, 927
StyI CCWWGG 2 cut(s) 319, 390
TaaI ACNGT 2 cut(s) 211, 247
TasI AATT 5 cut(s) 291, 525, 709, 767, 1140
TatI WGTACW 1 cut(s) 1154
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 5 cut(s) 131, 206, 1067, 1406, 1451
Tru9I TTAA 5 cut(s) 131, 206, 1067, 1406, 1451
TscAI CASTG 3 cut(s) 250, 1185, 1227
TseFI GTSAC 2 cut(s) 829, 1319
TseI GCWGC 3 cut(s) 77, 359, 1374
Tsp45I GTSAC 2 cut(s) 829, 1319
TspDTI ATGAA 5 cut(s) 148, 357, 939, 960, 1117
TspMI CCCGGG 1 cut(s) 927
TspRI CASTG 3 cut(s) 250, 1185, 1227
Van91I CCANNNNNTGG 1 cut(s) 1234
VpaK11BI GGWCC 1 cut(s) 1206
VspI ATTAAT 1 cut(s) 1067
XmaI CCCGGG 1 cut(s) 927
XmnI GAANNNNTTC 1 cut(s) 946
XspI CTAG 2 cut(s) 573, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.