Rroxscaffold_1G00009110

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001224
Physical Location & Seq
Reverse (-)
11495179 .. 11497145
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009110.1

Sequence Viewer

Length: 1305 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGGCATCATATGGCTTCAATCCATAAATGGAACAAACACAACCTTCCCTGCTTACTCATCCCAACTCAAGAACAGTCTCTCCATGTCCCAAATACAAATCAACAATCTCGTCTTCGCCTCCGACGCAGGGAAACTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCTAGTCCTCATAATCGGATCACTCCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAAACCAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCAGGCAATAGCGTCTGTTGGATCAACACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGCTTCATCAACAGGTTGCTGTGGGACTAACAACAAGTTACCAAGGATTGAGTGCAAAAATCTACACAGATATTGTCAGTGCTGCTTTTTCTTCTTTACCTCATAAGAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGCGGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCAAGAAACATGGCAACGGGGTTCAATTTAATATTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAATGATCGGCTTAGACAGAATGATAGGTACCGGAGTATTCCTACTGATGCCCTTGGTAATCCCAATTGTGGAAAAAATCAGAGAAAAAATATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTTGAGAGCATAGAGAGTGGTGTAGTGAAAGAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATAATGAGGTTGGTGAAGTACAGGTTCATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGACGTTGATGGTGCAAAGGATAGATTTTTGGTTATATTTCCTAGTTTATTTTTTCAGTGCAACAATTGGGCTCGTGTTCTTGAACACCTTGGGACAGATAGCAGAGTCAAGAGGCTCATCTAAGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGATTATTTCTTCGCAAGAAGCAAGTACATGATTTCAAGGCCTGCATTCCTAATGGCATTAGCGGCACCAATAGCAGGAGTTTTTTCTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

434

Amino Acids

47.71

Weight (kDa)

7.01

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 253 2.6e-71 Nodulin-like
NFD4_C PF23262 318 - 418 6.7e-41 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 719
AccB1I GGYRCC 3 cut(s) 568, 719, 1231
AciI CCGC 4 cut(s) 401, 560, 1229, 1295
AclWI GGATC 3 cut(s) 247, 368, 697
AcoI YGGCCR 1 cut(s) 1144
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 6 cut(s) 721, 821, 904, 936, 1193, 1293
AfiI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
AgsI TTSAA 4 cut(s) 59, 631, 1060, 1203
AhlI ACTAGT 1 cut(s) 550
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 2 cut(s) 20, 1270
AluI AGCT 2 cut(s) 20, 1270
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 3 cut(s) 122, 1154, 1281
AlwI GGATC 3 cut(s) 247, 368, 697
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 4 cut(s) 516, 847, 1144, 1205
ApeKI GCWGC 2 cut(s) 200, 482
Asp718I GGTACC 1 cut(s) 719
AsuHPI GGTGA 4 cut(s) 322, 589, 670, 941
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BanI GGYRCC 3 cut(s) 568, 719, 1231
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 2 cut(s) 187, 469
BccI CCATC 2 cut(s) 786, 979
BceAI ACGGC 3 cut(s) 280, 656, 1131
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 122, 1154, 1281
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 5 cut(s) 38, 224, 551, 800, 1019
BfmI CTRYAG 1 cut(s) 1301
BfuAI ACCTGC 1 cut(s) 901
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 3 cut(s) 201, 483, 1230
BlsI GCNGC 3 cut(s) 202, 484, 1231
BmeT110I CYCGRG 1 cut(s) 248
BmiI GGNNCC 5 cut(s) 570, 606, 721, 797, 1233
BmsI GCATC 2 cut(s) 54, 729
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 2 cut(s) 92, 872
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 5 cut(s) 247, 333, 442, 744, 1065
BsaWI WCCGGW 2 cut(s) 659, 722
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 760, 1241
Bse1I ACTGG 1 cut(s) 681
Bse3DI GCAATG 2 cut(s) 195, 651
BseDI CCNNGG 5 cut(s) 247, 333, 442, 744, 1065
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
BseMI GCAATG 2 cut(s) 195, 651
BseNI ACTGG 1 cut(s) 681
BseRI GAGGAG 2 cut(s) 236, 980
BseXI GCAGC 2 cut(s) 187, 469
BseYI CCCAGC 1 cut(s) 1266
BsgI GTGCAG 1 cut(s) 929
BshFI GGCC 4 cut(s) 518, 849, 1146, 1207
BshNI GGYRCC 3 cut(s) 568, 719, 1231
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 192, 660, 723
BslFI GGGAC 3 cut(s) 112, 438, 1083
BslI CCNNNNNNNGG 3 cut(s) 168, 760, 1241
BsmAI GTCTC 3 cut(s) 122, 1154, 1281
BsmBI CGTCTC 2 cut(s) 1154, 1281
BsmFI GGGAC 3 cut(s) 112, 438, 1083
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 4 cut(s) 518, 849, 1146, 1207
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 514, 1050
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 5 cut(s) 239, 360, 689, 696, 838
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 401, 560, 1229, 1295
BspANI GGCC 4 cut(s) 518, 849, 1146, 1207
BspLI GGNNCC 5 cut(s) 570, 606, 721, 797, 1233
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 3 cut(s) 247, 368, 697
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 3 cut(s) 568, 719, 1231
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 681
BssECI CCNNGG 5 cut(s) 247, 333, 442, 744, 1065
BssMI GATC 5 cut(s) 239, 360, 689, 696, 838
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 1049
BssT1I CCWWGG 4 cut(s) 333, 442, 744, 1065
Bst2BI CACGAG 1 cut(s) 1049
Bst4CI ACNGT 3 cut(s) 116, 370, 907
Bst6I CTCTTC 1 cut(s) 885
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 343, 1209
BstDEI CTNAG 2 cut(s) 703, 1098
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 5 cut(s) 242, 363, 692, 699, 841
BstMAI GTCTC 3 cut(s) 122, 1154, 1281
BstMBI GATC 5 cut(s) 239, 360, 689, 696, 838
BstMWI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
BstSFI CTRYAG 1 cut(s) 1301
BstV1I GCAGC 2 cut(s) 187, 469
BstV2I GAAGAC 1 cut(s) 145
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 4 cut(s) 518, 849, 1146, 1207
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsIMutI CAGTG 3 cut(s) 484, 912, 1039
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 343, 1209
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 6 cut(s) 720, 820, 903, 935, 1192, 1292
CviAII CATG 6 cut(s) 124, 334, 616, 947, 1153, 1195
CviQI GTAC 6 cut(s) 720, 820, 903, 935, 1192, 1292
DdeI CTNAG 2 cut(s) 703, 1098
DpnI GATC 5 cut(s) 241, 362, 691, 698, 840
DpnII GATC 5 cut(s) 239, 360, 689, 696, 838
EaeI YGGCCR 1 cut(s) 1144
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
Eco130I CCWWGG 4 cut(s) 333, 442, 744, 1065
Eco147I AGGCCT 2 cut(s) 518, 1207
Eco24I GRGCYC 1 cut(s) 1050
Eco88I CYCGRG 1 cut(s) 248
EcoT14I CCWWGG 4 cut(s) 333, 442, 744, 1065
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 4 cut(s) 333, 442, 744, 1065
Esp3I CGTCTC 2 cut(s) 1154, 1281
FaeI CATG 6 cut(s) 127, 337, 619, 950, 1156, 1198
FaqI GGGAC 3 cut(s) 112, 438, 1083
FatI CATG 6 cut(s) 123, 333, 615, 946, 1152, 1194
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 3 cut(s) 201, 483, 1230
FokI GGATG 1 cut(s) 85
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 3 cut(s) 201, 483, 1230
FspBI CTAG 5 cut(s) 38, 224, 551, 800, 1019
GluI GCNGC 3 cut(s) 201, 483, 1230
GsaI CCCAGC 1 cut(s) 1270
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 4 cut(s) 518, 849, 1146, 1207
HapII CCGG 3 cut(s) 192, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 127, 337, 619, 950, 1156, 1198
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 2 cut(s) 663, 1082
HpaII CCGG 3 cut(s) 192, 660, 723
HphI GGTGA 4 cut(s) 322, 589, 670, 941
Hpy166II GTNNAC 1 cut(s) 820
Hpy188I TCNGA 4 cut(s) 163, 239, 773, 843
Hpy188III TCNNGA 5 cut(s) 109, 786, 1057, 1086, 1166
Hpy8I GTNNAC 1 cut(s) 820
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 4 cut(s) 94, 221, 1141, 1167
HpyCH4III ACNGT 3 cut(s) 116, 370, 907
HpyCH4IV ACGT 2 cut(s) 980, 1274
HpyCH4V TGCA 5 cut(s) 455, 910, 991, 1037, 1211
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
HpyF3I CTNAG 2 cut(s) 703, 1098
HpySE526I ACGT 2 cut(s) 980, 1274
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 127, 337, 619, 950, 1156, 1198
KpnI GGTACC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 5 cut(s) 239, 360, 689, 696, 838
LmnI GCTCC 2 cut(s) 604, 795
Lsp1109I GCAGC 2 cut(s) 187, 469
LweI GCATC 2 cut(s) 54, 729
MaeI CTAG 5 cut(s) 38, 224, 551, 800, 1019
MaeII ACGT 2 cut(s) 980, 1274
MaeIII GTNAC 3 cut(s) 283, 437, 976
MalI GATC 5 cut(s) 241, 362, 691, 698, 840
MboI GATC 5 cut(s) 239, 360, 689, 696, 838
MfeI CAATTG 2 cut(s) 756, 1041
MhlI GDGCHC 2 cut(s) 514, 1050
MluCI AATT 4 cut(s) 272, 631, 756, 1041
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 2 cut(s) 186, 338
MseI TTAA 2 cut(s) 32, 635
MslI CAYNNNNRTG 2 cut(s) 335, 590
MspI CCGG 3 cut(s) 192, 660, 723
MunI CAATTG 2 cut(s) 756, 1041
Mva1269I GAATGC 1 cut(s) 1211
MwoI GCNNNNNNNGC 6 cut(s) 42, 164, 566, 855, 1229, 1238
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 5 cut(s) 239, 360, 689, 696, 838
NlaIII CATG 6 cut(s) 127, 337, 619, 950, 1156, 1198
NlaIV GGNNCC 5 cut(s) 570, 606, 721, 797, 1233
NmuCI GTSAC 1 cut(s) 976
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PceI AGGCCT 2 cut(s) 518, 1207
PctI GAATGC 1 cut(s) 1211
PfeI GAWTC 1 cut(s) 663
PkrI GCNGC 3 cut(s) 202, 484, 1231
PleI GAGTC 1 cut(s) 1090
PpsI GAGTC 1 cut(s) 1090
PspFI CCCAGC 1 cut(s) 1266
PspN4I GGNNCC 5 cut(s) 570, 606, 721, 797, 1233
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 6 cut(s) 721, 821, 904, 936, 1193, 1293
RsaNI GTAC 6 cut(s) 720, 820, 903, 935, 1192, 1292
RseI CAYNNNNRTG 2 cut(s) 335, 590
SaqAI TTAA 2 cut(s) 32, 635
SatI GCNGC 3 cut(s) 201, 483, 1230
Sau3AI GATC 5 cut(s) 239, 360, 689, 696, 838
SchI GAGTC 1 cut(s) 1091
SduI GDGCHC 2 cut(s) 514, 1050
SfaNI GCATC 2 cut(s) 54, 729
SfcI CTRYAG 1 cut(s) 1301
SfuI TTCGAA 1 cut(s) 391
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 2 cut(s) 107, 851
SmoI CTYRAG 2 cut(s) 107, 851
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 4 cut(s) 272, 631, 756, 1041
SseBI AGGCCT 2 cut(s) 518, 1207
SsiI CCGC 4 cut(s) 401, 560, 1229, 1295
SspI AATATT 2 cut(s) 321, 639
SspMI CTAG 5 cut(s) 38, 224, 551, 800, 1019
StuI AGGCCT 2 cut(s) 518, 1207
StyI CCWWGG 4 cut(s) 333, 442, 744, 1065
TaaI ACNGT 3 cut(s) 116, 370, 907
TaiI ACGT 2 cut(s) 983, 1277
TaqI TCGA 1 cut(s) 391
TasI AATT 4 cut(s) 272, 631, 756, 1041
TatI WGTACW 4 cut(s) 819, 902, 934, 1191
TauI GCSGC 1 cut(s) 1232
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 2 cut(s) 32, 635
Tru9I TTAA 2 cut(s) 32, 635
TscAI CASTG 3 cut(s) 484, 912, 1039
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 2 cut(s) 200, 482
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 3 cut(s) 395, 905, 932
TspRI CASTG 3 cut(s) 484, 912, 1039
XspI CTAG 5 cut(s) 38, 224, 551, 800, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.