AT3G05910

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001553
Physical Location & Seq
Reverse (-)
1764253 .. 1767843
3591 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05910.1

Sequence Viewer

Length: 1248 bp
ATGGTGAAGCTTTTGTTAGTAGGGTTTGTTGTGGCTGGTATAATACTGGGAACTCAAGCAAATGAATATTTAGATTTCAATGTGACAGAAATAGATCGCATTGAGGAATTAGAGTTTGGATTTTCCAAATATAGCTCCAATTTGAACCCTTTAATGGTTGGTCTCACCCTTATCAGAGGAGCTGATTCTGGAGCTGTTTGTTTGGATGGAACATTGCCTGGATATCACTTGCACCGTGGACATGGATCTGGAGCTAATAGCTGGCTCATTCAATTGGAGGGAGGAGGATGGTGCAACAACATCAGGACCTGTGTATACAGGAAGACTACTCGACGTGGTTCCTCTAATTATATGGAGAAACAGCTTCAGTTTACAGGAATACTGAGTGACAAAGCTCAAGAAAATCCTGACTTTTTCAACTGGAATAGAGTTAAGCTTCGTTACTGCGATGGCGCTTCTTTCAGTGGAGATGGCCAGAATCAGGCTGCACAACTTCAGTTTAGAGGAGAGCGTATCTGGAGAGCCGCAATAGATGATTTGAAGGCGAATGGAATGCGATACGCCAATCAGGCACTTCTCTCCGGATGCTCTGCTGGTGGTTTAGCAGCAATCTTGCGCTGCGATGAGTTTAGGAACTTATTCCCTGGATCAACCAAAGTCAAGTGCTTGAGTGATGCAGGCTTGTTCTTGGACACTGCCGATGTTTCAGGCGGCCGCACAATCAGGAACTTATACAATGGTGTCGTAGAATTGCAGAGTGTGAAGAACAATCTTCCACGTATATGCACAAACCATCTTGATCCAACCTCGTGTTTCTTCCCACAAAATCTGATCAGTCAAATGAAAACTCCACTTTTTATCGTCAACGCAGCATATGATACATGGCAGATTCAAAGCAGTATAGCTCCAACATCAGCTGATCCTAGTGGATTTTGGCATGACTGCAGACTAAACCATGGAAAATGTACTCCTGCACAGTTGAGATTCTTGCAAGGATTCAGGGAACAGATGCTTAGAGTAGTCAAGGGCTTCTCAATGTCTAGACAGAATGGATTGTTCATCAATTCTTGCTTTGCTCACTGCCAAACCGAGAGACAAGATACTTGGTTTGCCGATGATTCTCCTGTTATCAGGAAAAAGGCGGTGGCAATAGCTGTAGGAGATTGGTATTTCGATAGAGCGGAAGTGAAGTTAGTCGATTGCCCATACCCGTGTGACAAGAGCTGCCACAACTTGGTGTTCAGATGA

Protein Analysis

415

Amino Acids

46.33

Weight (kDa)

8.42

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 49 - 410 2.2e-169 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccBSI CCGCTC 1 cut(s) 1181
AccI GTMKAC 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 581
AciI CCGC 5 cut(s) 525, 711, 715, 1142, 1181
AclWI GGATC 4 cut(s) 253, 655, 794, 914
AcoI YGGCCR 2 cut(s) 472, 712
AcuI CTGAAG 2 cut(s) 350, 479
AfaI GTAC 1 cut(s) 967
AfiI CCNNNNNNNGG 3 cut(s) 154, 481, 1234
AgsI TTSAA 6 cut(s) 79, 145, 272, 418, 541, 893
AjiI CACGTC 1 cut(s) 335
AjnI CCWGG 2 cut(s) 217, 643
AjuI GAANNNNNNNTTGG 2 cut(s) 99, 131
Alw26I GTCTC 2 cut(s) 167, 1087
AlwI GGATC 4 cut(s) 253, 655, 794, 914
AlwNI CAGNNNCTG 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 581
AoxI GGCC 2 cut(s) 472, 712
ApeKI GCWGC 5 cut(s) 485, 605, 618, 869, 1224
Asp700I GAANNNNTTC 1 cut(s) 638
AspLEI GCGC 2 cut(s) 455, 618
AspS9I GGNCC 1 cut(s) 306
AsuHPI GGTGA 2 cut(s) 16, 157
AvaII GGWCC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 474
BauI CACGAG 1 cut(s) 808
BbsI GAAGAC 1 cut(s) 329
BbvI GCAGC 5 cut(s) 472, 605, 617, 881, 1211
BccI CCATC 5 cut(s) 200, 282, 443, 464, 801
BcgI CGANNNNNNTGC 2 cut(s) 535, 569
BciT130I CCWGG 2 cut(s) 219, 645
BclI TGATCA 1 cut(s) 831
BcoDI GTCTC 2 cut(s) 167, 1087
BfaI CTAG 2 cut(s) 924, 1041
BfmI CTRYAG 2 cut(s) 943, 1155
BfoI RGCGCY 1 cut(s) 456
BglI GCCNNNNNGGC 1 cut(s) 569
BisI GCNGC 8 cut(s) 486, 525, 606, 619, 712, 715, 870, 1225
BlsI GCNGC 8 cut(s) 487, 526, 607, 620, 713, 716, 871, 1226
Bme1390I CCNGG 2 cut(s) 219, 645
Bme18I GGWCC 1 cut(s) 306
BmgBI CACGTC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 306
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 219, 645
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 3 cut(s) 575, 664, 999
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 329
BpmI CTGGAG 3 cut(s) 210, 270, 538
BpuEI CTTGAG 3 cut(s) 39, 381, 688
BsaAI YACGTR 1 cut(s) 779
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 235, 643, 955
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 481, 1234
Bse1I ACTGG 2 cut(s) 51, 425
Bse3DI GCAATG 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 581
BseBI CCWGG 2 cut(s) 219, 645
BseDI CCNNGG 3 cut(s) 235, 643, 955
BseGI GGATG 3 cut(s) 211, 293, 590
BseLI CCNNNNNNNGG 3 cut(s) 154, 481, 1234
BseMI GCAATG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 51, 425
BseRI GAGGAG 3 cut(s) 192, 297, 519
BseX3I CGGCCG 1 cut(s) 712
BseXI GCAGC 5 cut(s) 472, 605, 617, 881, 1211
BsgI GTGCAG 2 cut(s) 471, 957
Bsh1285I CGRYCG 1 cut(s) 715
BshFI GGCC 2 cut(s) 474, 714
BsiEI CGRYCG 1 cut(s) 715
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 3 cut(s) 154, 481, 1234
BsmAI GTCTC 2 cut(s) 167, 1087
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 2 cut(s) 474, 714
Bso31I GGTCTC 1 cut(s) 167
Bsp13I TCCGGA 1 cut(s) 581
Bsp143I GATC 6 cut(s) 94, 245, 647, 799, 831, 919
Bsp19I CCATGG 1 cut(s) 955
BspACI CCGC 5 cut(s) 525, 711, 715, 1142, 1181
BspANI GGCC 2 cut(s) 474, 714
BspCNI CTCAG 1 cut(s) 375
BspEI TCCGGA 1 cut(s) 581
BspLI GGNNCC 1 cut(s) 340
BspMAI CTGCAG 1 cut(s) 947
BspPI GGATC 4 cut(s) 253, 655, 794, 914
BspTNI GGTCTC 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 1181
BsrDI GCAATG 1 cut(s) 212
BsrI ACTGG 2 cut(s) 51, 425
BssECI CCNNGG 3 cut(s) 235, 643, 955
BssMI GATC 6 cut(s) 94, 245, 647, 799, 831, 919
BssNAI GTATAC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 808
BssT1I CCWWGG 1 cut(s) 955
Bst1107I GTATAC 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 808
Bst2UI CCWGG 2 cut(s) 219, 645
Bst4CI ACNGT 2 cut(s) 236, 978
BstBAI YACGTR 1 cut(s) 779
BstC8I GCNNGC 2 cut(s) 263, 679
BstDEI CTNAG 2 cut(s) 383, 1013
BstDSI CCRYGG 2 cut(s) 235, 955
BstF5I GGATG 3 cut(s) 211, 293, 590
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 2 cut(s) 455, 618
BstKTI GATC 6 cut(s) 97, 248, 650, 802, 834, 922
BstMAI GTCTC 2 cut(s) 167, 1087
BstMBI GATC 6 cut(s) 94, 245, 647, 799, 831, 919
BstMCI CGRYCG 1 cut(s) 715
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNI CCWGG 2 cut(s) 219, 645
BstSCI CCNGG 2 cut(s) 217, 643
BstSFI CTRYAG 2 cut(s) 943, 1155
BstV1I GCAGC 5 cut(s) 472, 605, 617, 881, 1211
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BstZ17I GTATAC 1 cut(s) 316
BstZI CGGCCG 1 cut(s) 712
BsuRI GGCC 2 cut(s) 474, 714
BtgI CCRYGG 2 cut(s) 235, 955
BtgZI GCGATG 2 cut(s) 462, 636
BtrI CACGTC 1 cut(s) 335
BtsCI GGATG 3 cut(s) 211, 293, 590
BtsI GCAGTG 2 cut(s) 693, 1078
BtsIMutI CAGTG 3 cut(s) 469, 693, 1078
Cac8I GCNNGC 2 cut(s) 263, 679
CaiI CAGNNNCTG 1 cut(s) 309
CciNI GCGGCCGC 1 cut(s) 712
CfoI GCGC 2 cut(s) 455, 618
Cfr13I GGNCC 1 cut(s) 306
Csp6I GTAC 1 cut(s) 966
CviAII CATG 4 cut(s) 242, 882, 938, 956
CviQI GTAC 1 cut(s) 966
DdeI CTNAG 2 cut(s) 383, 1013
DpnI GATC 6 cut(s) 96, 247, 649, 801, 833, 921
DpnII GATC 6 cut(s) 94, 245, 647, 799, 831, 919
EaeI YGGCCR 2 cut(s) 472, 712
EagI CGGCCG 1 cut(s) 712
EclXI CGGCCG 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 955
Eco31I GGTCTC 1 cut(s) 167
Eco32I GATATC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 306
Eco52I CGGCCG 1 cut(s) 712
Eco57I CTGAAG 2 cut(s) 350, 479
EcoO109I RGGNCCY 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 217, 643
EcoRV GATATC 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 955
FaeI CATG 4 cut(s) 245, 885, 941, 959
FatI CATG 4 cut(s) 241, 881, 937, 955
FauNDI CATATG 1 cut(s) 874
FbaI TGATCA 1 cut(s) 831
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 8 cut(s) 486, 525, 606, 619, 712, 715, 870, 1225
FokI GGATG 3 cut(s) 218, 300, 597
Fsp4HI GCNGC 8 cut(s) 486, 525, 606, 619, 712, 715, 870, 1225
FspBI CTAG 2 cut(s) 924, 1041
GlaI GCGC 2 cut(s) 454, 617
GluI GCNGC 8 cut(s) 486, 525, 606, 619, 712, 715, 870, 1225
GsuI CTGGAG 3 cut(s) 210, 270, 538
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 2 cut(s) 474, 714
HapII CCGG 1 cut(s) 582
HhaI GCGC 2 cut(s) 455, 618
Hin1II CATG 4 cut(s) 245, 885, 941, 959
Hin6I GCGC 2 cut(s) 453, 616
HinP1I GCGC 2 cut(s) 453, 616
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HindIII AAGCTT 2 cut(s) 8, 434
HinfI GANTC 6 cut(s) 185, 478, 889, 984, 996, 1118
HpaII CCGG 1 cut(s) 582
HphI GGTGA 2 cut(s) 16, 157
Hpy166II GTNNAC 4 cut(s) 239, 316, 372, 865
Hpy188I TCNGA 3 cut(s) 176, 831, 1244
Hpy8I GTNNAC 4 cut(s) 239, 316, 372, 865
Hpy99I CGWCG 1 cut(s) 336
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 2 cut(s) 236, 978
HpyCH4IV ACGT 2 cut(s) 334, 778
HpyCH4V TGCA 9 cut(s) 232, 294, 488, 677, 754, 786, 945, 974, 991
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 2 cut(s) 383, 1013
HpySE526I ACGT 2 cut(s) 334, 778
Hsp92II CATG 4 cut(s) 245, 885, 941, 959
HspAI GCGC 2 cut(s) 453, 616
Kpn2I TCCGGA 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 831
Kzo9I GATC 6 cut(s) 94, 245, 647, 799, 831, 919
LmnI GCTCC 5 cut(s) 140, 179, 191, 251, 910
Lsp1109I GCAGC 5 cut(s) 472, 605, 617, 881, 1211
LweI GCATC 3 cut(s) 575, 664, 999
MaeI CTAG 2 cut(s) 924, 1041
MaeII ACGT 2 cut(s) 334, 778
MaeIII GTNAC 4 cut(s) 82, 386, 440, 1214
MalI GATC 6 cut(s) 96, 247, 649, 801, 833, 921
MbiI CCGCTC 1 cut(s) 1181
MboI GATC 6 cut(s) 94, 245, 647, 799, 831, 919
MboII GAAGA 4 cut(s) 334, 764, 775, 808
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 1 cut(s) 245
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 6 cut(s) 107, 139, 272, 346, 749, 1063
MluNI TGGCCA 1 cut(s) 474
MmeI TCCRAC 2 cut(s) 827, 932
MnlI CCTC 8 cut(s) 97, 170, 271, 275, 278, 352, 497, 817
Mox20I TGGCCA 1 cut(s) 474
MroI TCCGGA 1 cut(s) 581
MroXI GAANNNNTTC 1 cut(s) 638
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 152, 432
MslI CAYNNNNRTG 2 cut(s) 781, 1210
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 2 cut(s) 219, 645
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 2 cut(s) 219, 645
MwoI GCNNNNNNNGC 1 cut(s) 569
NcoI CCATGG 1 cut(s) 955
NdeI CATATG 1 cut(s) 874
NdeII GATC 6 cut(s) 94, 245, 647, 799, 831, 919
NlaIII CATG 4 cut(s) 245, 885, 941, 959
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 3 cut(s) 82, 386, 1214
NotI GCGGCCGC 1 cut(s) 712
PctI GAATGC 1 cut(s) 558
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 6 cut(s) 185, 478, 889, 984, 996, 1118
PflMI CCANNNNNTGG 1 cut(s) 1234
PkrI GCNGC 8 cut(s) 487, 526, 607, 620, 713, 716, 871, 1226
Ppu21I YACGTR 1 cut(s) 779
PpuMI RGGWCCY 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 217, 643
PspGI CCWGG 2 cut(s) 217, 643
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 306
PspPPI RGGWCCY 1 cut(s) 306
PstI CTGCAG 1 cut(s) 947
PstNI CAGNNNCTG 1 cut(s) 309
PsuI RGATCY 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 917
RsaI GTAC 1 cut(s) 967
RsaNI GTAC 1 cut(s) 966
RseI CAYNNNNRTG 2 cut(s) 781, 1210
SaqAI TTAA 2 cut(s) 152, 432
SatI GCNGC 8 cut(s) 486, 525, 606, 619, 712, 715, 870, 1225
Sau3AI GATC 6 cut(s) 94, 245, 647, 799, 831, 919
Sau96I GGNCC 1 cut(s) 306
ScrFI CCNGG 2 cut(s) 219, 645
SfaNI GCATC 3 cut(s) 575, 664, 999
SfcI CTRYAG 2 cut(s) 943, 1155
SinI GGWCC 1 cut(s) 306
SmiMI CAYNNNNRTG 2 cut(s) 781, 1210
SmlI CTYRAG 3 cut(s) 54, 396, 667
SmoI CTYRAG 3 cut(s) 54, 396, 667
Sse9I AATT 6 cut(s) 107, 139, 272, 346, 749, 1063
SsiI CCGC 5 cut(s) 525, 711, 715, 1142, 1181
SspI AATATT 1 cut(s) 68
SspMI CTAG 2 cut(s) 924, 1041
StyD4I CCNGG 2 cut(s) 217, 643
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 2 cut(s) 236, 978
TaiI ACGT 2 cut(s) 337, 781
TaqI TCGA 3 cut(s) 331, 1173, 1197
TasI AATT 6 cut(s) 107, 139, 272, 346, 749, 1063
TatI WGTACW 1 cut(s) 965
TauI GCSGC 3 cut(s) 527, 714, 717
TfiI GAWTC 6 cut(s) 185, 478, 889, 984, 996, 1118
Tru1I TTAA 2 cut(s) 152, 432
Tru9I TTAA 2 cut(s) 152, 432
TscAI CASTG 3 cut(s) 469, 700, 1085
TseFI GTSAC 3 cut(s) 82, 386, 1214
TseI GCWGC 5 cut(s) 485, 605, 618, 869, 1224
Tsp45I GTSAC 3 cut(s) 82, 386, 1214
TspDTI ATGAA 3 cut(s) 78, 857, 1048
TspRI CASTG 3 cut(s) 469, 700, 1085
Van91I CCANNNNNTGG 1 cut(s) 1234
VpaK11BI GGWCC 1 cut(s) 306
XbaI TCTAGA 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 2 cut(s) 924, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.