Rh3DG093100

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001553
Physical Location & Seq
Reverse (-)
7262648 .. 7264197
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG093100.1

Sequence Viewer

Length: 669 bp
ATGTTGAAGTTCTTGTGGGTTTGCATTGTAATAGCAACTTTTTTCAGCAACTGGGTTGATGGGTTTGTTCAGTACAGCCATGAAGGAGATCCCAAGACTGAAATCATTAATAACACTCTGATGGTATCACTAACCCTCATTCAAGGAGCTGATGCCAAAGGAGCAGTCTGCTTGGATGGAACATTACCTGCTTATTATTTTCATCGGGGACACGGGTCAGGGAAAAAGAGTTGGCTCGTTCACTTTCAGGGAGGAGGATGGTGTGGCAACATCACGGATTGTGTTGCTCGCAAGAAAACGCAGCTCGGATCATCAAATTTCATGCTACCAGAGACAGGATTCACCGGAATACTAAGCTATAAAGCTGAAGAAAATCCAGATTTCTTCAACTGGAATAGAGTAATGCTTCCCTCTTGTGACGGTGCTTCTTTTTCTGGGGACAGTGAAAATAAGGAAGCACAACTGCAATTTAGAGGACATCGCATTTGGTTGGCTGCAATGGAAAAATTGATGTCATTGGGCATGCGCCACGCCAAGAAGGCTCTTATTTCTGGTTGCTCTGCTGGAGGTCTGACATCTATTTTGCACTGTGATAAGTTCCGGGGATTATTTCGAAGAAATACTGAAGTAAAGTGCCTCAGTGATGCTGGATTTTTCCTTGACTCGTGA

Protein Analysis

222

Amino Acids

24.6

Weight (kDa)

8.4

Isoelectric Point (pI)

31.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 38 - 221 4.6e-87 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AclWI GGATC 2 cut(s) 83, 316
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 387, 645
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 3 cut(s) 7, 143, 388
AluBI AGCT 4 cut(s) 149, 304, 357, 365
AluI AGCT 4 cut(s) 149, 304, 357, 365
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 2 cut(s) 83, 316
AlwNI CAGNNNCTG 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 301, 494
ApoI RAATTY 1 cut(s) 316
ArsI GACNNNNNNTTYG 6 cut(s) 150, 182, 468, 500, 565, 597
AseI ATTAAT 1 cut(s) 108
AspLEI GCGC 1 cut(s) 528
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 613
BauI CACGAG 1 cut(s) 664
BbvI GCAGC 2 cut(s) 313, 481
BccI CCATC 4 cut(s) 53, 115, 170, 252
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 2 cut(s) 302, 495
BlsI GCNGC 2 cut(s) 303, 496
Bme1390I CCNGG 1 cut(s) 602
BmrFI CCNGG 1 cut(s) 602
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 2 cut(s) 142, 634
BmuI ACTGGG 1 cut(s) 61
BoxI GACNNNNGTC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 601
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 56, 395
Bse3DI GCAATG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 601
BseGI GGATG 2 cut(s) 181, 263
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 652
BseNI ACTGG 2 cut(s) 56, 395
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 2 cut(s) 313, 481
BsiSI CCGG 2 cut(s) 345, 601
BslFI GGGAC 2 cut(s) 222, 452
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 2 cut(s) 222, 452
Bsp119I TTCGAA 1 cut(s) 613
Bsp143I GATC 2 cut(s) 88, 308
BspCNI CTCAG 1 cut(s) 651
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 2 cut(s) 83, 316
BspT104I TTCGAA 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 504
BsrI ACTGG 2 cut(s) 56, 395
BssECI CCNNGG 1 cut(s) 601
BssMI GATC 2 cut(s) 88, 308
BssSI CACGAG 1 cut(s) 664
Bst2BI CACGAG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 422, 443, 590
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 289, 524
BstDEI CTNAG 2 cut(s) 353, 638
BstF5I GGATG 2 cut(s) 181, 263
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 2 cut(s) 91, 311
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 2 cut(s) 88, 308
BstMWI GCNNNNNNNGC 2 cut(s) 161, 539
BstNSI RCATGY 1 cut(s) 526
BstPAI GACNNNNGTC 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 313, 481
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 181, 263
BtsIMutI CAGTG 3 cut(s) 448, 586, 646
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 289, 524
CaiI CAGNNNCTG 1 cut(s) 51
CfoI GCGC 1 cut(s) 528
Csp6I GTAC 1 cut(s) 73
CviAII CATG 3 cut(s) 80, 322, 523
CviJI RGCY 8 cut(s) 78, 149, 235, 304, 357, 365, 494, 542
CviKI_1 RGCY 8 cut(s) 78, 149, 235, 304, 357, 365, 494, 542
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 2 cut(s) 353, 638
DpnI GATC 2 cut(s) 90, 310
DpnII GATC 2 cut(s) 88, 308
Eco57I CTGAAG 2 cut(s) 387, 645
FaeI CATG 3 cut(s) 83, 325, 526
FaiI YATR 4 cut(s) 81, 323, 360, 524
FaqI GGGAC 2 cut(s) 222, 452
FatI CATG 3 cut(s) 79, 321, 522
Fnu4HI GCNGC 2 cut(s) 302, 495
FokI GGATG 2 cut(s) 188, 270
Fsp4HI GCNGC 2 cut(s) 302, 495
GlaI GCGC 1 cut(s) 527
GluI GCNGC 2 cut(s) 302, 495
GsuI CTGGAG 1 cut(s) 585
HapII CCGG 2 cut(s) 345, 601
HhaI GCGC 1 cut(s) 528
Hin1II CATG 3 cut(s) 83, 325, 526
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HinfI GANTC 2 cut(s) 339, 662
HpaII CCGG 2 cut(s) 345, 601
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 120, 308, 573
Hpy188III TCNNGA 2 cut(s) 377, 666
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 2 cut(s) 77, 532
HpyCH4III ACNGT 3 cut(s) 422, 443, 590
HpyCH4V TGCA 4 cut(s) 24, 466, 497, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 539
HpyF3I CTNAG 2 cut(s) 353, 638
Hsp92II CATG 3 cut(s) 83, 325, 526
HspAI GCGC 1 cut(s) 526
Kzo9I GATC 2 cut(s) 88, 308
LmnI GCTCC 2 cut(s) 146, 161
Lsp1109I GCAGC 2 cut(s) 313, 481
LweI GCATC 2 cut(s) 142, 634
MaeIII GTNAC 1 cut(s) 416
MalI GATC 2 cut(s) 90, 310
MboI GATC 2 cut(s) 88, 308
MboII GAAGA 3 cut(s) 376, 380, 627
MflI RGATCY 1 cut(s) 88
MluCI AATT 3 cut(s) 316, 467, 506
MlyI GAGTC 1 cut(s) 656
MnlI CCTC 7 cut(s) 146, 245, 248, 421, 467, 560, 647
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 2 cut(s) 345, 601
MspR9I CCNGG 1 cut(s) 602
MwoI GCNNNNNNNGC 2 cut(s) 161, 539
NciI CCSGG 1 cut(s) 602
NdeII GATC 2 cut(s) 88, 308
NlaIII CATG 3 cut(s) 83, 325, 526
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 613
PaeI GCATGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 339
PkrI GCNGC 2 cut(s) 303, 496
PleI GAGTC 1 cut(s) 656
PpsI GAGTC 1 cut(s) 656
PshAI GACNNNNGTC 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 108
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 302, 495
Sau3AI GATC 2 cut(s) 88, 308
SchI GAGTC 1 cut(s) 656
ScrFI CCNGG 1 cut(s) 602
SetI ASST 6 cut(s) 151, 190, 306, 359, 367, 571
SfaNI GCATC 2 cut(s) 142, 634
SfuI TTCGAA 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 119
SphI GCATGC 1 cut(s) 526
Sse9I AATT 3 cut(s) 316, 467, 506
StyD4I CCNGG 1 cut(s) 600
TaaI ACNGT 3 cut(s) 422, 443, 590
TaqI TCGA 1 cut(s) 613
TasI AATT 3 cut(s) 316, 467, 506
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 3 cut(s) 448, 593, 646
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 301, 494
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 3 cut(s) 96, 191, 310
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 3 cut(s) 448, 593, 646
VspI ATTAAT 1 cut(s) 108
XapI RAATTY 1 cut(s) 316
XceI RCATGY 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.