MD12G1198300.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001553
Physical Location & Seq
Forward (+)
27936385 .. 27938419
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1198300.v1.1.491

Sequence Viewer

Length: 987 bp
ATGGTGAACTTCTTTTGGGTTGGCTTTGTACCAGCACTTGTTTTCAGAACTTGGGTTGATAGGTTTGTGGAAGAGAAGAGTCTTGATGCGACGGTGCCTTCTTTGTTGGAGACCTATGGGAGCTCCAAGTCTGCAAATGACATACTCATGGTAGAGATAACACTCGTCGAAGGAGCTGCTGCCCAAGGAGCAGTCTGCCTGGATGGAACAGTACCTGCTTATCATTTGCATCGTGGATATGGATCAGGGGTGAACAGTTGGCTCATTGACCTTCAGGGAGGAGCATGGTGTAATGACACCAAAAGTTGTTCTAACCGCAAATTTTCAGCGTATGGATCATCAGCACACATGGAAAAAGAGAGAGCATTTTCTGGAATACTAAGCAATAAATCTGAAGAAAATCCCGAGTTTTTCAACTGGAATAGAGTAGAACTTCGCTATTGTGATGGCGCTTCATTTTCTGGGGACAGTGAAAATCAGGAAGCACAACTGCAATTTAGAGGACAGCGTATATGGTTATCTGCAATGGACGATTTGATGTCAAAGGGAATGCGCAACGCGAATCATGCTCTTCTTTCTGGTTGCTCTGCCGGTGGTCTGTCATCCATTTTGCACTGTGATGAGTTTCAGAGCTTGTTTCCCACAACTACAAAAGTGAAGTGCCTGAGTGATGCTGGATTTTTTCTCGACACGGTTGATATTTCTGGTGGACGCACACTCAGGGATTATTATAGTGGTGTTGTAAGCTTGCAGGGGGTGGAAGAAAATCTGCCGCGATTCTGTACTAGTCACCTCGACCCTACTTCGGTAATCAATTTTTGTGCACTTTTAAATTTGAAAATAGGTGATAGCATAATAAGTTTTACACTACTTCTTGCTTTGCAGTGCTTCTTTCCTGAGAACTTAATCGCGAACATTACAACCCCGCTCTTTATACTCAATGCAGCATATGATTCATGGCAGGTAATTTTTCATTATTCATTTTAA

Protein Analysis

329

Amino Acids

36.29

Weight (kDa)

4.99

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 48 - 322 4e-111 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 554
Acc36I ACCTGC 2 cut(s) 223, 952
AccB1I GGYRCC 1 cut(s) 94
AccBSI CCGCTC 1 cut(s) 928
AccII CGCG 3 cut(s) 560, 775, 911
AciI CCGC 3 cut(s) 316, 773, 926
AclWI GGATC 2 cut(s) 250, 343
AcsI RAATTY 2 cut(s) 320, 832
AcuI CTGAAG 2 cut(s) 257, 414
AfaI GTAC 3 cut(s) 30, 213, 784
AfiI CCNNNNNNNGG 1 cut(s) 805
AgsI TTSAA 2 cut(s) 415, 838
AhlI ACTAGT 1 cut(s) 785
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 4 cut(s) 123, 176, 633, 747
AluI AGCT 4 cut(s) 123, 176, 633, 747
Alw21I GWGCWC 2 cut(s) 125, 826
Alw26I GTCTC 1 cut(s) 104
Alw44I GTGCAC 1 cut(s) 822
AlwI GGATC 2 cut(s) 250, 343
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 404
ApaLI GTGCAC 1 cut(s) 822
ApeKI GCWGC 3 cut(s) 176, 179, 944
ApoI RAATTY 2 cut(s) 320, 832
AspLEI GCGC 2 cut(s) 452, 555
AsuHPI GGTGA 4 cut(s) 16, 262, 782, 857
AvaI CYCGRG 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 826
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 125, 826
BbvI GCAGC 3 cut(s) 163, 166, 956
BccI CCATC 2 cut(s) 197, 440
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 104
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 453
BfuAI ACCTGC 2 cut(s) 223, 952
BisI GCNGC 4 cut(s) 177, 180, 773, 945
BlsI GCNGC 4 cut(s) 178, 181, 774, 946
Bme1390I CCNGG 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 3 cut(s) 76, 238, 661
BsaBI GATNNNNATC 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 241
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 2 cut(s) 208, 602
BseJI GATNNNNATC 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 3 cut(s) 656, 733, 888
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 826
BseXI GCAGC 3 cut(s) 163, 166, 956
Bsh1236I CGCG 3 cut(s) 560, 775, 911
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 125, 826
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 591
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 555
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 125, 826
Bsp143I GATC 2 cut(s) 242, 335
Bsp68I TCGCGA 1 cut(s) 911
BspACI CCGC 3 cut(s) 316, 773, 926
BspCNI CTCAG 3 cut(s) 657, 732, 889
BspFNI CGCG 3 cut(s) 560, 775, 911
BspLI GGNNCC 1 cut(s) 96
BspMI ACCTGC 2 cut(s) 223, 952
BspPI GGATC 2 cut(s) 250, 343
BspQI GCTCTTC 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 928
BsrDI GCAATG 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 590
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 2 cut(s) 242, 335
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 6 cut(s) 94, 211, 257, 470, 617, 694
Bst6I CTCTTC 3 cut(s) 66, 71, 576
BstC8I GCNNGC 1 cut(s) 749
BstDEI CTNAG 4 cut(s) 380, 665, 719, 897
BstF5I GGATG 2 cut(s) 208, 602
BstFNI CGCG 3 cut(s) 560, 775, 911
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 2 cut(s) 452, 555
BstKTI GATC 2 cut(s) 245, 338
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 2 cut(s) 242, 335
BstMWI GCNNNNNNNGC 2 cut(s) 188, 566
BstNI CCWGG 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 826
BstUI CGCG 3 cut(s) 560, 775, 911
BstV1I GCAGC 3 cut(s) 163, 166, 956
BtsCI GGATG 2 cut(s) 208, 602
BtsI GCAGTG 1 cut(s) 890
BtsIMutI CAGTG 3 cut(s) 475, 613, 890
BtuMI TCGCGA 1 cut(s) 911
BveI ACCTGC 2 cut(s) 223, 952
Cac8I GCNNGC 1 cut(s) 749
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 2 cut(s) 452, 555
Cfr10I RCCGGY 1 cut(s) 590
CseI GACGC 1 cut(s) 720
Csp6I GTAC 3 cut(s) 29, 212, 783
CviAII CATG 5 cut(s) 148, 285, 349, 566, 957
CviJI RGCY 6 cut(s) 24, 123, 176, 262, 633, 747
CviKI_1 RGCY 6 cut(s) 24, 123, 176, 262, 633, 747
CviQI GTAC 3 cut(s) 29, 212, 783
DdeI CTNAG 4 cut(s) 380, 665, 719, 897
DpnI GATC 2 cut(s) 244, 337
DpnII GATC 2 cut(s) 242, 335
DraI TTTAAA 1 cut(s) 831
Eam1104I CTCTTC 3 cut(s) 66, 71, 576
EarI CTCTTC 3 cut(s) 66, 71, 576
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 257, 414
Eco88I CYCGRG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 123
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 5 cut(s) 151, 288, 352, 569, 960
FaqI GGGAC 1 cut(s) 479
FatI CATG 5 cut(s) 147, 284, 348, 565, 956
FauI CCCGC 1 cut(s) 933
FauNDI CATATG 1 cut(s) 949
Fnu4HI GCNGC 4 cut(s) 177, 180, 773, 945
FokI GGATG 2 cut(s) 215, 589
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 4 cut(s) 177, 180, 773, 945
FspBI CTAG 1 cut(s) 786
FspI TGCGCA 1 cut(s) 554
GlaI GCGC 2 cut(s) 451, 554
GluI GCNGC 4 cut(s) 177, 180, 773, 945
HaeII RGCGCY 1 cut(s) 453
HapII CCGG 1 cut(s) 591
HgaI GACGC 1 cut(s) 720
HhaI GCGC 2 cut(s) 452, 555
Hin1II CATG 5 cut(s) 151, 288, 352, 569, 960
Hin6I GCGC 2 cut(s) 450, 553
HinP1I GCGC 2 cut(s) 450, 553
HindIII AAGCTT 1 cut(s) 745
HinfI GANTC 4 cut(s) 79, 562, 777, 953
HpaII CCGG 1 cut(s) 591
HphI GGTGA 4 cut(s) 16, 262, 782, 857
Hpy166II GTNNAC 4 cut(s) 7, 253, 710, 824
Hpy188I TCNGA 3 cut(s) 47, 394, 630
Hpy188III TCNNGA 7 cut(s) 83, 372, 404, 479, 686, 896, 910
Hpy8I GTNNAC 4 cut(s) 7, 253, 710, 824
Hpy99I CGWCG 2 cut(s) 94, 170
HpyAV CCTTC 3 cut(s) 108, 164, 281
HpyCH4III ACNGT 6 cut(s) 94, 211, 257, 470, 617, 694
HpyCH4V TGCA 9 cut(s) 134, 229, 493, 524, 613, 751, 824, 883, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 566
HpyF3I CTNAG 4 cut(s) 380, 665, 719, 897
Hsp92II CATG 5 cut(s) 151, 288, 352, 569, 960
HspAI GCGC 2 cut(s) 450, 553
Kzo9I GATC 2 cut(s) 242, 335
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 5 cut(s) 120, 128, 173, 188, 281
Lsp1109I GCAGC 3 cut(s) 163, 166, 956
LweI GCATC 3 cut(s) 76, 238, 661
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 1 cut(s) 788
MalI GATC 2 cut(s) 244, 337
MbiI CCGCTC 1 cut(s) 928
MboI GATC 2 cut(s) 242, 335
MboII GAAGA 5 cut(s) 83, 88, 407, 563, 773
MhlI GDGCHC 2 cut(s) 125, 826
MluCI AATT 5 cut(s) 320, 494, 814, 832, 966
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 3 cut(s) 272, 494, 803
MseI TTAA 3 cut(s) 830, 905, 985
MslI CAYNNNNRTG 2 cut(s) 146, 618
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 3 cut(s) 560, 775, 911
MwoI GCNNNNNNNGC 2 cut(s) 188, 566
NdeI CATATG 1 cut(s) 949
NdeII GATC 2 cut(s) 242, 335
NlaIII CATG 5 cut(s) 151, 288, 352, 569, 960
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 788
NruI TCGCGA 1 cut(s) 911
NsbI TGCGCA 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 576
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 562, 777, 953
PkrI GCNGC 4 cut(s) 178, 181, 774, 946
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 96
PstNI CAGNNNCTG 1 cut(s) 215
RruI TCGCGA 1 cut(s) 911
RsaI GTAC 3 cut(s) 30, 213, 784
RsaNI GTAC 3 cut(s) 29, 212, 783
RseI CAYNNNNRTG 2 cut(s) 146, 618
SacI GAGCTC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 3 cut(s) 830, 905, 985
SatI GCNGC 4 cut(s) 177, 180, 773, 945
Sau3AI GATC 2 cut(s) 242, 335
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 125, 826
SfaNI GCATC 3 cut(s) 76, 238, 661
SmiMI CAYNNNNRTG 2 cut(s) 146, 618
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 5 cut(s) 320, 494, 814, 832, 966
SsiI CCGC 3 cut(s) 316, 773, 926
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 6 cut(s) 94, 211, 257, 470, 617, 694
TaqI TCGA 3 cut(s) 168, 687, 795
TasI AATT 5 cut(s) 320, 494, 814, 832, 966
TatI WGTACW 1 cut(s) 782
TauI GCSGC 1 cut(s) 775
TfiI GAWTC 3 cut(s) 562, 777, 953
Tru1I TTAA 3 cut(s) 830, 905, 985
Tru9I TTAA 3 cut(s) 830, 905, 985
TscAI CASTG 3 cut(s) 475, 620, 890
TseFI GTSAC 1 cut(s) 788
TseI GCWGC 3 cut(s) 176, 179, 944
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 4 cut(s) 444, 945, 962, 969
TspRI CASTG 3 cut(s) 475, 620, 890
VneI GTGCAC 1 cut(s) 822
XapI RAATTY 2 cut(s) 320, 832
XspI CTAG 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.