Rorug03G0031600

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001553
Physical Location & Seq
Reverse (-)
2477730 .. 2478707
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0031600.1

Sequence Viewer

Length: 978 bp
ATGGAGCAGATAAACGAATTTGAAGGAGGAGGAGTAGCCATCGGACTAAGTTGCTTTCACATGCAAGCAGACCCAACTTGTGCGACCCTACTCATCAAGTCATGGGCCGAGACTCACCGCCGGGAACCCGTCGTACACCCGCCTTCCTTCGACGACTTATCGGACTCGCCACTCACTAGCGGACTCAACATTCACAAGACAAGCACAAAGTTATCATGCTCTTATTACGAAGCCAAGTCCATGGCAGCACAGACCCCATTAGTACTGAAGAAAATGGGCACAGCCACTTTCAAATTTTCCAATGAAGTGATCAAGCAAAGCCTCTTAGAAATCCAGCAAACATGCCCTGTTGCAACCCCGTTTGATTTGCTGGCCGCACTCTTTTGGGCACGCATTGCTCGCTTGAAGCACCCAAAAAGTGAAAGCAAGCACTCTCTCTCCATTTGTGTCGACTTCAGGAAGAAGCTGCAGCTCTCTTATGGGCACTACGGCAATGCCTTGCATTTTTCGTTGCTTTCATTACCTGATGTGGAAGAAAAGGAAGACTTGGGAGATGTGGTTGGAGCCGTGCACCGCCATGTGTCGAGTCAGGGGGAGGGGGAGTTTTGGTCTGCTTTAGACTGGTTTGAATCACGAAAGGAAGAGGGAGGTCAGTTTGGGGCACCTTTTAGAATGTATGGCCCCGAGTTGACATGTATGAGCATGGAGCATATGGTTGACTTTCCTGGGATTACTGGGAATAAGCCGTTGATGTATACAGCTGTGTTTGATAAGGAGAACAGGCCCGTACACGTGTCATATCATGTTGGAAATGTGGAAGGGGAAGGTTTGATAATCGTGATGCCATCTCCGGAAGGAGGGCTGGCGAGGATGGTTACGGTGACATTGCCGGAGGAGGAACTAGAGCAGCTATGTCAGGATGAAGCTATTTCGAACTTAAACCCAATAATGGTCATAAGTGGGACGAGAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

325

Amino Acids

36.01

Weight (kDa)

5.54

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 307 3.7e-19 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 661
AccI GTMKAC 2 cut(s) 450, 755
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 5 cut(s) 118, 140, 180, 375, 574
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 2 cut(s) 17, 293
AcuI CTGAAG 2 cut(s) 287, 439
AcvI CACGTG 1 cut(s) 793
AfaI GTAC 3 cut(s) 135, 264, 789
AfiI CCNNNNNNNGG 2 cut(s) 857, 949
AflIII ACRYGT 3 cut(s) 692, 790, 792
AgsI TTSAA 4 cut(s) 23, 292, 406, 629
AjnI CCWGG 1 cut(s) 724
AluBI AGCT 5 cut(s) 466, 472, 761, 910, 926
AluI AGCT 5 cut(s) 466, 472, 761, 910, 926
Alw21I GWGCWC 1 cut(s) 573
Alw26I GTCTC 1 cut(s) 104
Alw44I GTGCAC 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 683
Aor13HI TCCGGA 1 cut(s) 850
AoxI GGCC 4 cut(s) 105, 372, 679, 782
ApaLI GTGCAC 1 cut(s) 569
ApeKI GCWGC 4 cut(s) 245, 466, 469, 907
ApoI RAATTY 2 cut(s) 17, 293
AspS9I GGNCC 3 cut(s) 105, 680, 783
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 107, 892
AsuII TTCGAA 1 cut(s) 932
AvaI CYCGRG 1 cut(s) 683
BaeGI GKGCMC 5 cut(s) 281, 391, 486, 573, 664
BanI GGYRCC 1 cut(s) 661
BbrPI CACGTG 1 cut(s) 793
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 573
BbvI GCAGC 4 cut(s) 257, 453, 481, 919
BccI CCATC 3 cut(s) 47, 853, 865
BceAI ACGGC 3 cut(s) 505, 551, 730
BciT130I CCWGG 1 cut(s) 726
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 2 cut(s) 177, 902
BfmI CTRYAG 1 cut(s) 467
BisI GCNGC 5 cut(s) 246, 375, 467, 470, 908
BlsI GCNGC 5 cut(s) 247, 376, 468, 471, 909
BmcAI AGTACT 1 cut(s) 264
Bme1390I CCNGG 2 cut(s) 122, 726
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 3 cut(s) 105, 680, 783
BmiI GGNNCC 4 cut(s) 126, 565, 663, 682
BmrFI CCNGG 2 cut(s) 122, 726
BmrI ACTGGG 1 cut(s) 744
BmsI GCATC 1 cut(s) 831
BmuI ACTGGG 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 932
BpuMI CCSGG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 793
BsaJI CCNNGG 2 cut(s) 240, 725
BsaWI WCCGGW 1 cut(s) 850
Bsc4I CCNNNNNNNGG 2 cut(s) 857, 949
Bse1I ACTGG 2 cut(s) 626, 739
Bse3DI GCAATG 3 cut(s) 393, 499, 884
BseAI TCCGGA 1 cut(s) 850
BseBI CCWGG 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 240, 725
BseGI GGATG 2 cut(s) 876, 925
BseLI CCNNNNNNNGG 2 cut(s) 857, 949
BseMI GCAATG 3 cut(s) 393, 499, 884
BseNI ACTGG 2 cut(s) 626, 739
BseRI GAGGAG 3 cut(s) 42, 45, 908
BseSI GKGCMC 5 cut(s) 281, 391, 486, 573, 664
BseXI GCAGC 4 cut(s) 257, 453, 481, 919
BshFI GGCC 4 cut(s) 107, 374, 681, 784
BshNI GGYRCC 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 573
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 3 cut(s) 121, 851, 890
BslI CCNNNNNNNGG 2 cut(s) 857, 949
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 4 cut(s) 107, 374, 681, 784
BsoBI CYCGRG 1 cut(s) 683
Bsp119I TTCGAA 1 cut(s) 932
Bsp1286I GDGCHC 5 cut(s) 281, 391, 486, 573, 664
Bsp13I TCCGGA 1 cut(s) 850
Bsp143I GATC 1 cut(s) 309
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 5 cut(s) 118, 140, 180, 375, 574
BspANI GGCC 4 cut(s) 107, 374, 681, 784
BspEI TCCGGA 1 cut(s) 850
BspLI GGNNCC 4 cut(s) 126, 565, 663, 682
BspMAI CTGCAG 1 cut(s) 471
BspT104I TTCGAA 1 cut(s) 932
BspT107I GGYRCC 1 cut(s) 661
BsrDI GCAATG 3 cut(s) 393, 499, 884
BsrI ACTGG 2 cut(s) 626, 739
BssECI CCNNGG 2 cut(s) 240, 725
BssMI GATC 1 cut(s) 309
BssNAI GTATAC 1 cut(s) 756
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 880
Bst6I CTCTTC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 932
BstC8I GCNNGC 6 cut(s) 66, 372, 391, 400, 428, 864
BstDEI CTNAG 2 cut(s) 47, 325
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 2 cut(s) 876, 925
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 395, 399
BstNI CCWGG 1 cut(s) 726
BstNSI RCATGY 3 cut(s) 64, 345, 696
BstSCI CCNGG 2 cut(s) 120, 724
BstSFI CTRYAG 1 cut(s) 467
BstSLI GKGCMC 5 cut(s) 281, 391, 486, 573, 664
BstV1I GCAGC 4 cut(s) 257, 453, 481, 919
BstV2I GAAGAC 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 241
BstZ17I GTATAC 1 cut(s) 756
BsuRI GGCC 4 cut(s) 107, 374, 681, 784
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 876, 925
Cac8I GCNNGC 6 cut(s) 66, 372, 391, 400, 428, 864
Cfr13I GGNCC 3 cut(s) 105, 680, 783
Csp6I GTAC 3 cut(s) 134, 263, 788
CviAII CATG 9 cut(s) 61, 102, 216, 241, 342, 578, 693, 703, 803
CviQI GTAC 3 cut(s) 134, 263, 788
DdeI CTNAG 2 cut(s) 47, 325
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 240
Eco57I CTGAAG 2 cut(s) 287, 439
Eco72I CACGTG 1 cut(s) 793
Eco88I CYCGRG 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 724
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 9 cut(s) 64, 105, 219, 244, 345, 581, 696, 706, 806
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FatI CATG 9 cut(s) 60, 101, 215, 240, 341, 577, 692, 702, 802
FauI CCCGC 1 cut(s) 147
FauNDI CATATG 1 cut(s) 711
FbaI TGATCA 1 cut(s) 309
FblI GTMKAC 2 cut(s) 450, 755
Fnu4HI GCNGC 5 cut(s) 246, 375, 467, 470, 908
FokI GGATG 2 cut(s) 883, 932
Fsp4HI GCNGC 5 cut(s) 246, 375, 467, 470, 908
FspBI CTAG 2 cut(s) 177, 902
GluI GCNGC 5 cut(s) 246, 375, 467, 470, 908
HaeIII GGCC 4 cut(s) 107, 374, 681, 784
HapII CCGG 3 cut(s) 121, 851, 890
Hin1II CATG 9 cut(s) 64, 105, 219, 244, 345, 581, 696, 706, 806
HincII GTYRAC 3 cut(s) 451, 690, 718
HindII GTYRAC 3 cut(s) 451, 690, 718
HinfI GANTC 5 cut(s) 112, 164, 183, 586, 629
HpaII CCGG 3 cut(s) 121, 851, 890
HphI GGTGA 2 cut(s) 107, 892
Hpy166II GTNNAC 7 cut(s) 136, 451, 571, 690, 718, 756, 790
Hpy188I TCNGA 2 cut(s) 44, 163
Hpy188III TCNNGA 5 cut(s) 457, 633, 838, 851, 917
Hpy8I GTNNAC 7 cut(s) 136, 451, 571, 690, 718, 756, 790
Hpy99I CGWCG 2 cut(s) 134, 155
HpyAV CCTTC 6 cut(s) 17, 153, 157, 812, 818, 848
HpyCH4III ACNGT 1 cut(s) 880
HpyCH4IV ACGT 1 cut(s) 792
HpyCH4V TGCA 5 cut(s) 64, 353, 469, 502, 571
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 399
HpyF3I CTNAG 2 cut(s) 47, 325
HpySE526I ACGT 1 cut(s) 792
Hsp92II CATG 9 cut(s) 64, 105, 219, 244, 345, 581, 696, 706, 806
Kpn2I TCCGGA 1 cut(s) 850
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 3 cut(s) 4, 563, 706
Lsp1109I GCAGC 4 cut(s) 257, 453, 481, 919
LweI GCATC 1 cut(s) 831
MaeI CTAG 2 cut(s) 177, 902
MaeII ACGT 1 cut(s) 792
MaeIII GTNAC 2 cut(s) 874, 880
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MboII GAAGA 5 cut(s) 280, 472, 545, 554, 653
MhlI GDGCHC 5 cut(s) 281, 391, 486, 573, 664
MluCI AATT 2 cut(s) 17, 293
MlyI GAGTC 4 cut(s) 106, 158, 177, 595
MmeI TCCRAC 2 cut(s) 541, 787
MroI TCCGGA 1 cut(s) 850
MseI TTAA 1 cut(s) 938
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 3 cut(s) 121, 851, 890
MspR9I CCNGG 2 cut(s) 122, 726
MvaI CCWGG 1 cut(s) 726
MwoI GCNNNNNNNGC 2 cut(s) 395, 399
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 711
NdeII GATC 1 cut(s) 309
NlaIII CATG 9 cut(s) 64, 105, 219, 244, 345, 581, 696, 706, 806
NlaIV GGNNCC 4 cut(s) 126, 565, 663, 682
NmeAIII GCCGAG 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 880
NspI RCATGY 3 cut(s) 64, 345, 696
NspV TTCGAA 1 cut(s) 932
PciI ACATGT 1 cut(s) 692
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 5 cut(s) 247, 376, 468, 471, 909
PleI GAGTC 4 cut(s) 106, 158, 177, 594
PmaCI CACGTG 1 cut(s) 793
PmlI CACGTG 1 cut(s) 793
PpsI GAGTC 4 cut(s) 106, 158, 177, 594
Ppu21I YACGTR 1 cut(s) 793
PscI ACATGT 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 724
PspCI CACGTG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 724
PspN4I GGNNCC 4 cut(s) 126, 565, 663, 682
PspPI GGNCC 3 cut(s) 105, 680, 783
PsrI GAACNNNNNNTAC 2 cut(s) 117, 149
PstI CTGCAG 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 761
RsaI GTAC 3 cut(s) 135, 264, 789
RsaNI GTAC 3 cut(s) 134, 263, 788
RseI CAYNNNNRTG 1 cut(s) 576
SalI GTCGAC 1 cut(s) 449
SaqAI TTAA 1 cut(s) 938
SatI GCNGC 5 cut(s) 246, 375, 467, 470, 908
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 3 cut(s) 105, 680, 783
ScaI AGTACT 1 cut(s) 264
SchI GAGTC 4 cut(s) 106, 158, 177, 595
ScrFI CCNGG 2 cut(s) 122, 726
SduI GDGCHC 5 cut(s) 281, 391, 486, 573, 664
SfaNI GCATC 1 cut(s) 831
SfcI CTRYAG 1 cut(s) 467
SfuI TTCGAA 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 576
Sse9I AATT 2 cut(s) 17, 293
SsiI CCGC 5 cut(s) 118, 140, 180, 375, 574
SspMI CTAG 2 cut(s) 177, 902
StyD4I CCNGG 2 cut(s) 120, 724
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 880
TaiI ACGT 1 cut(s) 795
TaqI TCGA 4 cut(s) 150, 450, 584, 932
TasI AATT 2 cut(s) 17, 293
TatI WGTACW 1 cut(s) 262
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 1 cut(s) 938
Tru9I TTAA 1 cut(s) 938
TseFI GTSAC 1 cut(s) 880
TseI GCWGC 4 cut(s) 245, 466, 469, 907
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 3 cut(s) 318, 507, 936
VneI GTGCAC 1 cut(s) 569
XapI RAATTY 2 cut(s) 17, 293
XceI RCATGY 3 cut(s) 64, 345, 696
XmiI GTMKAC 2 cut(s) 450, 755
XspI CTAG 2 cut(s) 177, 902
ZrmI AGTACT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.