Rh3BG092000

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001553
Physical Location & Seq
Reverse (-)
7255114 .. 7270355
15242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG092000.1

Sequence Viewer

Length: 1425 bp
ATGTTGAAGTTCTTGTGGGTTTGCATTGTAATAGCAACTTTTTTCAGCAACTGGGTTGATGGGTTTGTTCAGTACAGCCATGAAGGAGATCCCAAGACTGAAATCATTAATAACACTCTGATGGTATCACTAACCCTCATTCAAGGAGCTGATGCCAAAGGAGCAGTCTGCTTGGATGGAACATTACCTGCTTATTATTTTCATCGGGGACACGGGTCAGGGAAAAAGAGTTGGCTCGTTCACTTTCAGGGAGGAGGATGGTGTGGCAACATCACGGATTGTGTTGCTCGCAAGAAAACGCAGCTCGGATCATCAAATTTCATGCTACCAGAGACAGGATTCACCGGACTACTAAGCTATAAAGCTGAAGAAAATCCAGATTTCTTCAACTGGAATAGAGTAATGCTTCCCTCTTGTGACGGTGCTTCTTTTTCTGGGGACAGTGAAAATAAGGAAGCACAACTGCAATTTAGAGGACATCGCATTTGGTTGGCTGCAATGGAAAAATTGATGTCATTGGGCATGCGCCACGCCAAGAAGGCTCTTATTTCTGGTTGCTCTGCTGGAGGTCTGACATCTATTTTGCACTGTGATAAGTTCCGGGGATTATTTCGAAGAAATACTGAAGTGAAGTGCCTCAGTGATGCTGGATTTTTCCTTGACTCGGTTGACATATCTGGTGCACCAACATTCAGGAATTTTTTCAGTAATGTTGTAAGCTTGCAGGGGGTGAAAGAAAATCTGCCACACTTTTGTACTAGTCGCCTCGATCCTACTTCGTGCTTCTTCCCTCAGAACTTACTTGCCGGCATTAAAACCCCGCTGTTTATACTTAACTCAGCATATGATTCTTTTCAGTTCCAATTCAGCTTAGTCCCAGCATCAGCTGATCCCAACGGCTTATGGAACAGTTGCAAATTAAACGTTACCAACTGTTCCCCAACTCAGTTGCATATTCTGCAAGGATTCAGGAGTCAAATGCTACAGGCACTAAAGCCATTCTCAAAGTCTAATCAAAGTGGGATATTTATAGATTCATGCTATGCACACTGTCAGTCTCAGTTTCAGCTAACGTGGTATGCTGCCAATTCTACAATCATTGGAAACAAGACAATCTCACAGTCCGTCGGAGATTGGTATTTCGACCGTGCAGAAGTTAAGGAGTTTCTTAGCCTTGATCTGGGATTCTGGGTTGAAGTTTTTCAGATTGAAGTTGTTCAGGGGTTCTGCAAATCGCTGGTGGTGCGGATTTGCAAGTCTGATGCAATGGTGGATTGGCTGGCCGCCGGACCTAACGGTGGTGGAGGCAGCACAGCAACGGTATGTGATGTTGTTGCATGGGTGGAAAAGCCGGCCACCAGAGCTGTGGTCAAACGAGATGCCGCCAGAGGCCTTGACGGTGGTGGAGGCAAAGGAGCAGTGTGA

Protein Analysis

474

Amino Acids

52.02

Weight (kDa)

8.24

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 38 - 388 1.4e-152 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AciI CCGC 4 cut(s) 821, 1246, 1284, 1383
AclI AACGTT 1 cut(s) 924
AclWI GGATC 4 cut(s) 83, 316, 764, 884
AcoI YGGCCR 2 cut(s) 1281, 1353
AcsI RAATTY 2 cut(s) 316, 697
AcuI CTGAAG 2 cut(s) 387, 645
AfaI GTAC 2 cut(s) 74, 757
AfiI CCNNNNNNNGG 4 cut(s) 335, 664, 1180, 1298
AgsI TTSAA 5 cut(s) 7, 143, 388, 1196, 1211
AhlI ACTAGT 1 cut(s) 758
AluBI AGCT 9 cut(s) 149, 304, 357, 365, 720, 870, 887, 1069, 1364
AluI AGCT 9 cut(s) 149, 304, 357, 365, 720, 870, 887, 1069, 1364
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 2 cut(s) 326, 1062
Alw44I GTGCAC 1 cut(s) 681
AlwI GGATC 4 cut(s) 83, 316, 764, 884
AlwNI CAGNNNCTG 1 cut(s) 51
AoxI GGCC 3 cut(s) 1281, 1353, 1390
ApaLI GTGCAC 1 cut(s) 681
ApeKI GCWGC 4 cut(s) 301, 494, 1082, 1308
ApoI RAATTY 2 cut(s) 316, 697
ArsI GACNNNNNNTTYG 6 cut(s) 150, 182, 468, 500, 565, 597
AseI ATTAAT 1 cut(s) 108
Asp700I GAANNNNTTC 3 cut(s) 701, 1200, 1215
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 1289
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 2 cut(s) 334, 742
AsuII TTCGAA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 1289
BaeGI GKGCMC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 4 cut(s) 313, 481, 1069, 1320
BccI CCATC 4 cut(s) 53, 115, 170, 252
BceAI ACGGC 1 cut(s) 913
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 2 cut(s) 326, 1062
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 1 cut(s) 759
BfmI CTRYAG 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 6 cut(s) 302, 495, 1083, 1284, 1309, 1383
BlsI GCNGC 6 cut(s) 303, 496, 1084, 1285, 1310, 1384
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 1 cut(s) 1289
BmgT120I GGNCC 1 cut(s) 1289
BmrFI CCNGG 1 cut(s) 602
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 5 cut(s) 142, 634, 890, 1252, 1369
BmuI ACTGGG 1 cut(s) 61
BoxI GACNNNNGTC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 601
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 664, 1180, 1298
Bse118I RCCGGY 2 cut(s) 806, 1351
Bse1I ACTGG 2 cut(s) 56, 395
Bse3DI GCAATG 2 cut(s) 504, 1272
BseDI CCNNGG 1 cut(s) 601
BseGI GGATG 2 cut(s) 181, 263
BseLI CCNNNNNNNGG 4 cut(s) 335, 664, 1180, 1298
BseMI GCAATG 2 cut(s) 504, 1272
BseMII CTCAG 5 cut(s) 652, 806, 852, 959, 1073
BseNI ACTGG 2 cut(s) 56, 395
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 4 cut(s) 313, 481, 1069, 1320
BseYI CCCAGC 1 cut(s) 877
BsgI GTGCAG 1 cut(s) 1170
Bsh1285I CGRYCG 1 cut(s) 1147
BshFI GGCC 3 cut(s) 1283, 1355, 1392
BsiEI CGRYCG 1 cut(s) 1147
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 5 cut(s) 345, 601, 807, 1287, 1352
BslFI GGGAC 3 cut(s) 222, 452, 860
BslI CCNNNNNNNGG 4 cut(s) 335, 664, 1180, 1298
BsmAI GTCTC 2 cut(s) 326, 1062
BsmFI GGGAC 3 cut(s) 222, 452, 860
BsnI GGCC 3 cut(s) 1283, 1355, 1392
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 685
Bsp143I GATC 5 cut(s) 88, 308, 769, 889, 1177
BspACI CCGC 4 cut(s) 821, 1246, 1284, 1383
BspANI GGCC 3 cut(s) 1283, 1355, 1392
BspCNI CTCAG 5 cut(s) 651, 805, 851, 958, 1072
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 4 cut(s) 83, 316, 764, 884
BspT104I TTCGAA 1 cut(s) 613
BsrDI GCAATG 2 cut(s) 504, 1272
BsrFI RCCGGY 2 cut(s) 806, 1351
BsrI ACTGG 2 cut(s) 56, 395
BssAI RCCGGY 2 cut(s) 806, 1351
BssECI CCNNGG 1 cut(s) 601
BssMI GATC 5 cut(s) 88, 308, 769, 889, 1177
BstAPI GCANNNNNTGC 1 cut(s) 958
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 6 cut(s) 289, 524, 722, 808, 1281, 1353
BstDEI CTNAG 8 cut(s) 353, 638, 792, 838, 871, 945, 1059, 1169
BstF5I GGATG 2 cut(s) 181, 263
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 5 cut(s) 91, 311, 772, 892, 1180
BstMAI GTCTC 2 cut(s) 326, 1062
BstMBI GATC 5 cut(s) 88, 308, 769, 889, 1177
BstMCI CGRYCG 1 cut(s) 1147
BstMWI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1243, 1361
BstNSI RCATGY 1 cut(s) 526
BstPAI GACNNNNGTC 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 983
BstSLI GKGCMC 1 cut(s) 685
BstV1I GCAGC 4 cut(s) 313, 481, 1069, 1320
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 1366
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 3 cut(s) 1283, 1355, 1392
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 181, 263
BtsI GCAGTG 1 cut(s) 1425
BtsIMutI CAGTG 5 cut(s) 448, 586, 646, 1048, 1425
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 6 cut(s) 289, 524, 722, 808, 1281, 1353
CaiI CAGNNNCTG 1 cut(s) 51
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 2 cut(s) 806, 1351
Cfr13I GGNCC 1 cut(s) 1289
Csp6I GTAC 2 cut(s) 73, 756
CviAII CATG 5 cut(s) 80, 322, 523, 1038, 1338
CviQI GTAC 2 cut(s) 73, 756
DdeI CTNAG 8 cut(s) 353, 638, 792, 838, 871, 945, 1059, 1169
DpnI GATC 5 cut(s) 90, 310, 771, 891, 1179
DpnII GATC 5 cut(s) 88, 308, 769, 889, 1177
EaeI YGGCCR 2 cut(s) 1281, 1353
Eco147I AGGCCT 1 cut(s) 1392
Eco47I GGWCC 1 cut(s) 1289
Eco57I CTGAAG 2 cut(s) 387, 645
FaeI CATG 5 cut(s) 83, 325, 526, 1041, 1341
FaqI GGGAC 3 cut(s) 222, 452, 860
FatI CATG 5 cut(s) 79, 321, 522, 1037, 1337
FauI CCCGC 1 cut(s) 828
FauNDI CATATG 1 cut(s) 844
Fnu4HI GCNGC 6 cut(s) 302, 495, 1083, 1284, 1309, 1383
FokI GGATG 2 cut(s) 188, 270
Fsp4HI GCNGC 6 cut(s) 302, 495, 1083, 1284, 1309, 1383
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 527
GluI GCNGC 6 cut(s) 302, 495, 1083, 1284, 1309, 1383
GsaI CCCAGC 1 cut(s) 881
GsuI CTGGAG 1 cut(s) 585
HaeIII GGCC 3 cut(s) 1283, 1355, 1392
HapII CCGG 5 cut(s) 345, 601, 807, 1287, 1352
HhaI GCGC 1 cut(s) 528
Hin1II CATG 5 cut(s) 83, 325, 526, 1041, 1341
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 7 cut(s) 339, 662, 848, 966, 973, 1034, 1185
HpaII CCGG 5 cut(s) 345, 601, 807, 1287, 1352
HphI GGTGA 2 cut(s) 334, 742
Hpy166II GTNNAC 3 cut(s) 241, 670, 683
Hpy188I TCNGA 7 cut(s) 120, 308, 573, 795, 1130, 1206, 1261
Hpy188III TCNNGA 3 cut(s) 377, 694, 970
Hpy8I GTNNAC 3 cut(s) 241, 670, 683
Hpy99I CGWCG 1 cut(s) 1130
HpyAV CCTTC 2 cut(s) 77, 532
HpyCH4IV ACGT 2 cut(s) 924, 1073
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1243, 1361
HpyF3I CTNAG 8 cut(s) 353, 638, 792, 838, 871, 945, 1059, 1169
HpySE526I ACGT 2 cut(s) 924, 1073
Hsp92II CATG 5 cut(s) 83, 325, 526, 1041, 1341
HspAI GCGC 1 cut(s) 526
KroI GCCGGC 2 cut(s) 806, 1351
KroNI GCCGGC 2 cut(s) 808, 1353
Kzo9I GATC 5 cut(s) 88, 308, 769, 889, 1177
LmnI GCTCC 3 cut(s) 146, 161, 1415
Lsp1109I GCAGC 4 cut(s) 313, 481, 1069, 1320
LweI GCATC 5 cut(s) 142, 634, 890, 1252, 1369
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 924, 1073
MaeIII GTNAC 2 cut(s) 416, 925
MalI GATC 5 cut(s) 90, 310, 771, 891, 1179
MboI GATC 5 cut(s) 88, 308, 769, 889, 1177
MboII GAAGA 4 cut(s) 376, 380, 627, 778
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
MlyI GAGTC 2 cut(s) 656, 982
MmeI TCCRAC 1 cut(s) 1108
MroNI GCCGGC 2 cut(s) 806, 1351
MroXI GAANNNNTTC 3 cut(s) 701, 1200, 1215
MseI TTAA 5 cut(s) 108, 813, 834, 920, 1158
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 823, 887
MspI CCGG 5 cut(s) 345, 601, 807, 1287, 1352
MspR9I CCNGG 1 cut(s) 602
MwoI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1243, 1361
NaeI GCCGGC 2 cut(s) 808, 1353
NciI CCSGG 1 cut(s) 602
NdeI CATATG 1 cut(s) 844
NdeII GATC 5 cut(s) 88, 308, 769, 889, 1177
NgoMIV GCCGGC 2 cut(s) 806, 1351
NlaIII CATG 5 cut(s) 83, 325, 526, 1041, 1341
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 613
PaeI GCATGC 1 cut(s) 526
PceI AGGCCT 1 cut(s) 1392
PdiI GCCGGC 2 cut(s) 808, 1353
PdmI GAANNNNTTC 3 cut(s) 701, 1200, 1215
PfeI GAWTC 5 cut(s) 339, 848, 966, 1034, 1185
PkrI GCNGC 6 cut(s) 303, 496, 1084, 1285, 1310, 1384
PleI GAGTC 2 cut(s) 656, 981
PpsI GAGTC 2 cut(s) 656, 981
PshAI GACNNNNGTC 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 108
Psp1406I AACGTT 1 cut(s) 924
PspFI CCCAGC 1 cut(s) 877
PspPI GGNCC 1 cut(s) 1289
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 2 cut(s) 74, 757
RsaNI GTAC 2 cut(s) 73, 756
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 5 cut(s) 108, 813, 834, 920, 1158
SatI GCNGC 6 cut(s) 302, 495, 1083, 1284, 1309, 1383
Sau3AI GATC 5 cut(s) 88, 308, 769, 889, 1177
Sau96I GGNCC 1 cut(s) 1289
SchI GAGTC 2 cut(s) 656, 982
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 5 cut(s) 142, 634, 890, 1252, 1369
SfcI CTRYAG 1 cut(s) 983
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 1 cut(s) 1289
SmiMI CAYNNNNRTG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 758
SphI GCATGC 1 cut(s) 526
Sse9I AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
SseBI AGGCCT 1 cut(s) 1392
SsiI CCGC 4 cut(s) 821, 1246, 1284, 1383
SspMI CTAG 1 cut(s) 759
StuI AGGCCT 1 cut(s) 1392
StyD4I CCNGG 1 cut(s) 600
TaiI ACGT 2 cut(s) 927, 1076
TaqI TCGA 3 cut(s) 613, 768, 1143
TasI AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
TatI WGTACW 2 cut(s) 72, 755
TauI GCSGC 2 cut(s) 1286, 1385
TfiI GAWTC 5 cut(s) 339, 848, 966, 1034, 1185
Tru1I TTAA 5 cut(s) 108, 813, 834, 920, 1158
Tru9I TTAA 5 cut(s) 108, 813, 834, 920, 1158
TscAI CASTG 5 cut(s) 448, 593, 646, 1055, 1425
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 4 cut(s) 301, 494, 1082, 1308
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 4 cut(s) 96, 191, 310, 1026
TspGWI ACGGA 2 cut(s) 290, 1114
TspRI CASTG 5 cut(s) 448, 593, 646, 1055, 1425
VneI GTGCAC 1 cut(s) 681
VpaK11BI GGWCC 1 cut(s) 1289
VspI ATTAAT 1 cut(s) 108
XapI RAATTY 2 cut(s) 316, 697
XceI RCATGY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XmnI GAANNNNTTC 3 cut(s) 701, 1200, 1215
XspI CTAG 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.