MD12G1198000.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001553
Physical Location & Seq
Forward (+)
27925755 .. 27929002
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1198000.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGTGAAGTTCTTGTGGGTTGGCATTGTGGCAGCACTTGTTTCCAGAGTTCGGGCTGATGGGTTTGTGGAGAACAGTATCAATGAGGCGGTGCCGCCTTTGTGGGAGGAGACCTATGGAAGCTCCAAGGCTGCAAATGACATATTGATGGTAGGGCTAACCCTCGTCGAAGGAGCTGCTGCTAAAGGAGCAGTATGCTTGGATGGAACAGTTCCAGCGTATCATTTGCACCGCGGATATGGATCGGGGGTGAATAGTTGGCTCATTAAGTTTCAGGGAGGAGCATGGTGTAATGATACCAGAAGCTGCTCTGACCGCAAACTTACACCATTTGGATCATCAACGCGCATGGAAACAAAGATGCCATTTTCTGGAATACTAAGCAATCAATCTGAACAAAATCCTGAGTTTTTCAACTGGAATAGAGTACTGCTTCGCTATTGTGACGGCGCTTCATTTTCTGGGGACAGCGAAAATAAGAAAGAACATCTGCAATATAGGGGACAGCGGATATGGTTGTCTGCAATGGAAGATTTGATGTCAAAGGGCATGTGCGACGCCAATCAGGCTCTTCTTTTTGGTTGCTCTTCCGGCGGTCTGTCATCTATTCTGCACTGTGATGAATTTCGGGGCTTATTTCCCACAACTACAAAAGTGAAGTGCTTGAGTGATGGTGGATTTTTTATTGACGCAGTTGACATCTCCGGTGGACACACACTCAGGAATATTTATACCGGTGTTGTAAGCTTGCAGGGGGTGCGAGAAAATCTGCCGCGATTCTGTACTAGTCACCTTGACCCTACTTCGTGCTTCTTTCCTGAGAACTTAATCGCGAACATTACAACCCCACTCTTTATACTCAATTCAGCATATGATTCATGGCAGATCTATAACAGTTTAGCTCCACCATCAGCAGATCCCCATGGTGAGTGGAACAGTTGCAGATTAAACCTTGCAAGTTGTTCAGCATCACAGATGCAAATTCTGCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAATTTTCGGTGTCTAATCAAACTGGGATGTTCATAAATTCGTGTTTTGCGCACTGTCAAACGGGGCAAAGTGAATGGTTTTCACATGATTCTCCAGCTGTTGGAAACAAGACAATTGCAGAAGCTGTCGGAGATTGGTATTTTGATCGAGCGGAGGTTAAGGTTGTTGATGAAACTTGCCGCCATCTGGCTTTCGAATGA

Protein Analysis

408

Amino Acids

44.87

Weight (kDa)

5.71

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 48 - 401 2.9e-156 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1074
AccB1I GGYRCC 1 cut(s) 91
AccB7I CCANNNNNTGG 2 cut(s) 371, 1124
AccBSI CCGCTC 1 cut(s) 1175
AccII CGCG 4 cut(s) 234, 346, 775, 833
AclWI GGATC 3 cut(s) 250, 343, 911
AcsI RAATTY 3 cut(s) 623, 981, 1060
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 2 cut(s) 429, 784
AfiI CCNNNNNNNGG 4 cut(s) 51, 371, 1124, 1210
AgeI ACCGGT 1 cut(s) 734
AgsI TTSAA 1 cut(s) 415
AhlI ACTAGT 1 cut(s) 785
AluBI AGCT 7 cut(s) 123, 176, 306, 747, 902, 1121, 1148
AluI AGCT 7 cut(s) 123, 176, 306, 747, 902, 1121, 1148
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 3 cut(s) 250, 343, 911
AlwNI CAGNNNCTG 2 cut(s) 306, 1148
ApeKI GCWGC 5 cut(s) 32, 131, 176, 179, 306
ApoI RAATTY 3 cut(s) 623, 981, 1060
AsiGI ACCGGT 1 cut(s) 734
AspLEI GCGC 3 cut(s) 348, 452, 1075
AsuHPI GGTGA 4 cut(s) 16, 262, 782, 938
AsuII TTCGAA 1 cut(s) 1218
BanI GGYRCC 1 cut(s) 91
BbvI GCAGC 5 cut(s) 44, 118, 163, 166, 293
BccI CCATC 6 cut(s) 53, 142, 197, 665, 916, 1215
BceAI ACGGC 1 cut(s) 463
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BcoDI GTCTC 1 cut(s) 104
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 1 cut(s) 786
BfoI RGCGCY 1 cut(s) 453
BglI GCCNNNNNGGC 1 cut(s) 566
BglII AGATCT 1 cut(s) 885
BisI GCNGC 8 cut(s) 33, 95, 132, 177, 180, 307, 773, 1204
BlsI GCNGC 8 cut(s) 34, 96, 133, 178, 181, 308, 774, 1205
BmcAI AGTACT 1 cut(s) 429
BmiI GGNNCC 1 cut(s) 93
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 3 cut(s) 351, 966, 977
BmuI ACTGGG 1 cut(s) 1056
BpmI CTGGAG 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1218
BpuEI CTTGAG 1 cut(s) 685
BsaBI GATNNNNATC 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 126, 232, 922
BsaWI WCCGGW 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 371, 1124, 1210
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 2 cut(s) 422, 1051
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 241
BseDI CCNNGG 3 cut(s) 126, 232, 922
BseGI GGATG 2 cut(s) 208, 1056
BseJI GATNNNNATC 1 cut(s) 241
BseLI CCNNNNNNNGG 4 cut(s) 51, 371, 1124, 1210
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 3 cut(s) 396, 733, 810
BseNI ACTGG 2 cut(s) 422, 1051
BseRI GAGGAG 2 cut(s) 122, 294
BseXI GCAGC 5 cut(s) 44, 118, 163, 166, 293
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 4 cut(s) 234, 346, 775, 833
BshNI GGYRCC 1 cut(s) 91
BshTI ACCGGT 1 cut(s) 734
BsiSI CCGG 3 cut(s) 591, 705, 735
BslFI GGGAC 2 cut(s) 479, 516
BslI CCNNNNNNNGG 4 cut(s) 51, 371, 1124, 1210
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 2 cut(s) 479, 516
BsmI GAATGC 1 cut(s) 1018
Bso31I GGTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 1218
Bsp143I GATC 5 cut(s) 242, 335, 885, 916, 1168
Bsp19I CCATGG 1 cut(s) 922
Bsp68I TCGCGA 1 cut(s) 833
BspCNI CTCAG 3 cut(s) 397, 732, 811
BspFNI CGCG 4 cut(s) 234, 346, 775, 833
BspLI GGNNCC 1 cut(s) 93
BspPI GGATC 3 cut(s) 250, 343, 911
BspQI GCTCTTC 2 cut(s) 576, 592
BspT104I TTCGAA 1 cut(s) 1218
BspT107I GGYRCC 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 1175
BsrDI GCAATG 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 2 cut(s) 422, 1051
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 3 cut(s) 126, 232, 922
BssMI GATC 5 cut(s) 242, 335, 885, 916, 1168
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 2 cut(s) 126, 922
Bst4CI ACNGT 6 cut(s) 77, 211, 617, 896, 938, 1079
Bst6I CTCTTC 2 cut(s) 576, 592
BstACI GRCGYC 1 cut(s) 558
BstAPI GCANNNNNTGC 2 cut(s) 757, 985
BstBI TTCGAA 1 cut(s) 1218
BstC8I GCNNGC 1 cut(s) 749
BstDEI CTNAG 4 cut(s) 380, 405, 719, 819
BstDSI CCRYGG 2 cut(s) 232, 922
BstF5I GGATG 2 cut(s) 208, 1056
BstFNI CGCG 4 cut(s) 234, 346, 775, 833
BstH2I RGCGCY 1 cut(s) 453
BstHHI GCGC 3 cut(s) 348, 452, 1075
BstKTI GATC 5 cut(s) 245, 338, 888, 919, 1171
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 5 cut(s) 242, 335, 885, 916, 1168
BstMWI GCNNNNNNNGC 6 cut(s) 188, 315, 566, 591, 757, 985
BstNSI RCATGY 1 cut(s) 553
BstUI CGCG 4 cut(s) 234, 346, 775, 833
BstV1I GCAGC 5 cut(s) 44, 118, 163, 166, 293
BstX2I RGATCY 2 cut(s) 885, 916
BstYI RGATCY 2 cut(s) 885, 916
BtgI CCRYGG 2 cut(s) 232, 922
BtsCI GGATG 2 cut(s) 208, 1056
BtsIMutI CAGTG 2 cut(s) 613, 1075
BtuMI TCGCGA 1 cut(s) 833
Cac8I GCNNGC 1 cut(s) 749
CaiI CAGNNNCTG 2 cut(s) 306, 1148
CfoI GCGC 3 cut(s) 348, 452, 1075
Cfr10I RCCGGY 1 cut(s) 734
Cfr42I CCGCGG 1 cut(s) 235
CseI GACGC 2 cut(s) 566, 698
Csp6I GTAC 2 cut(s) 428, 783
CspAI ACCGGT 1 cut(s) 734
CviAII CATG 6 cut(s) 285, 349, 550, 879, 923, 1109
CviQI GTAC 2 cut(s) 428, 783
DdeI CTNAG 4 cut(s) 380, 405, 719, 819
DpnI GATC 5 cut(s) 244, 337, 887, 918, 1170
DpnII GATC 5 cut(s) 242, 335, 885, 916, 1168
Eam1104I CTCTTC 2 cut(s) 576, 592
EarI CTCTTC 2 cut(s) 576, 592
Eco130I CCWWGG 2 cut(s) 126, 922
Eco31I GGTCTC 1 cut(s) 104
EcoT14I CCWWGG 2 cut(s) 126, 922
ErhI CCWWGG 2 cut(s) 126, 922
FaeI CATG 6 cut(s) 288, 352, 553, 882, 926, 1112
FaqI GGGAC 2 cut(s) 479, 516
FatI CATG 6 cut(s) 284, 348, 549, 878, 922, 1108
FauNDI CATATG 1 cut(s) 871
Fnu4HI GCNGC 8 cut(s) 33, 95, 132, 177, 180, 307, 773, 1204
FokI GGATG 2 cut(s) 215, 1063
Fsp4HI GCNGC 8 cut(s) 33, 95, 132, 177, 180, 307, 773, 1204
FspBI CTAG 1 cut(s) 786
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 3 cut(s) 347, 451, 1074
GluI GCNGC 8 cut(s) 33, 95, 132, 177, 180, 307, 773, 1204
GsuI CTGGAG 1 cut(s) 1101
HaeII RGCGCY 1 cut(s) 453
HapII CCGG 3 cut(s) 591, 705, 735
HgaI GACGC 2 cut(s) 566, 698
HhaI GCGC 3 cut(s) 348, 452, 1075
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 6 cut(s) 288, 352, 553, 882, 926, 1112
Hin6I GCGC 3 cut(s) 346, 450, 1073
HinP1I GCGC 3 cut(s) 346, 450, 1073
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 1 cut(s) 745
HinfI GANTC 5 cut(s) 777, 875, 993, 1000, 1112
HpaII CCGG 3 cut(s) 591, 705, 735
HphI GGTGA 4 cut(s) 16, 262, 782, 938
Hpy166II GTNNAC 2 cut(s) 697, 710
Hpy188I TCNGA 3 cut(s) 313, 394, 1154
Hpy188III TCNNGA 7 cut(s) 45, 372, 404, 721, 818, 832, 997
Hpy8I GTNNAC 2 cut(s) 697, 710
Hpy99I CGWCG 2 cut(s) 170, 560
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 6 cut(s) 77, 211, 617, 896, 938, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 315, 566, 591, 757, 985
HpyF3I CTNAG 4 cut(s) 380, 405, 719, 819
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 6 cut(s) 288, 352, 553, 882, 926, 1112
HspAI GCGC 3 cut(s) 346, 450, 1073
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 5 cut(s) 242, 335, 885, 916, 1168
LguI GCTCTTC 2 cut(s) 576, 592
LmnI GCTCC 5 cut(s) 128, 173, 188, 281, 907
Lsp1109I GCAGC 5 cut(s) 44, 118, 163, 166, 293
LweI GCATC 3 cut(s) 351, 966, 977
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 2 cut(s) 443, 788
MalI GATC 5 cut(s) 244, 337, 887, 918, 1170
MbiI CCGCTC 1 cut(s) 1175
MboI GATC 5 cut(s) 242, 335, 885, 916, 1168
MboII GAAGA 3 cut(s) 542, 563, 579
MfeI CAATTG 1 cut(s) 1137
MflI RGATCY 2 cut(s) 885, 916
MluCI AATT 6 cut(s) 623, 862, 981, 1025, 1060, 1137
MmeI TCCRAC 2 cut(s) 1105, 1132
MnlI CCTC 5 cut(s) 79, 100, 173, 272, 1171
MseI TTAA 4 cut(s) 267, 827, 947, 1182
MslI CAYNNNNRTG 2 cut(s) 146, 618
MspA1I CMGCKG 3 cut(s) 234, 508, 1121
MspI CCGG 3 cut(s) 591, 705, 735
MunI CAATTG 1 cut(s) 1137
Mva1269I GAATGC 1 cut(s) 1018
MvnI CGCG 4 cut(s) 234, 346, 775, 833
MwoI GCNNNNNNNGC 6 cut(s) 188, 315, 566, 591, 757, 985
NcoI CCATGG 1 cut(s) 922
NdeI CATATG 1 cut(s) 871
NdeII GATC 5 cut(s) 242, 335, 885, 916, 1168
NlaIII CATG 6 cut(s) 288, 352, 553, 882, 926, 1112
NlaIV GGNNCC 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 443, 788
NruI TCGCGA 1 cut(s) 833
NsbI TGCGCA 1 cut(s) 1074
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 1218
PciSI GCTCTTC 2 cut(s) 576, 592
PctI GAATGC 1 cut(s) 1018
PfeI GAWTC 5 cut(s) 777, 875, 993, 1000, 1112
PflMI CCANNNNNTGG 2 cut(s) 371, 1124
PinAI ACCGGT 1 cut(s) 734
PkrI GCNGC 8 cut(s) 34, 96, 133, 178, 181, 308, 774, 1205
PspN4I GGNNCC 1 cut(s) 93
PstNI CAGNNNCTG 2 cut(s) 306, 1148
PsuI RGATCY 2 cut(s) 885, 916
PvuII CAGCTG 1 cut(s) 1121
RruI TCGCGA 1 cut(s) 833
RsaI GTAC 2 cut(s) 429, 784
RsaNI GTAC 2 cut(s) 428, 783
RseI CAYNNNNRTG 2 cut(s) 146, 618
SacII CCGCGG 1 cut(s) 235
SapI GCTCTTC 2 cut(s) 576, 592
SaqAI TTAA 4 cut(s) 267, 827, 947, 1182
SatI GCNGC 8 cut(s) 33, 95, 132, 177, 180, 307, 773, 1204
Sau3AI GATC 5 cut(s) 242, 335, 885, 916, 1168
ScaI AGTACT 1 cut(s) 429
SfaNI GCATC 3 cut(s) 351, 966, 977
Sfr303I CCGCGG 1 cut(s) 235
SfuI TTCGAA 1 cut(s) 1218
SgrBI CCGCGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 146, 618
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 6 cut(s) 623, 862, 981, 1025, 1060, 1137
SspI AATATT 1 cut(s) 727
SspMI CTAG 1 cut(s) 786
StyI CCWWGG 2 cut(s) 126, 922
TaaI ACNGT 6 cut(s) 77, 211, 617, 896, 938, 1079
TaqI TCGA 3 cut(s) 168, 1171, 1218
TasI AATT 6 cut(s) 623, 862, 981, 1025, 1060, 1137
TatI WGTACW 2 cut(s) 427, 782
TauI GCSGC 3 cut(s) 97, 775, 1206
TfiI GAWTC 5 cut(s) 777, 875, 993, 1000, 1112
Tru1I TTAA 4 cut(s) 267, 827, 947, 1182
Tru9I TTAA 4 cut(s) 267, 827, 947, 1182
TscAI CASTG 2 cut(s) 620, 1082
TseFI GTSAC 2 cut(s) 443, 788
TseI GCWGC 5 cut(s) 32, 131, 176, 179, 306
Tsp45I GTSAC 2 cut(s) 443, 788
TspDTI ATGAA 5 cut(s) 444, 636, 867, 1045, 1209
TspRI CASTG 2 cut(s) 620, 1082
Van91I CCANNNNNTGG 2 cut(s) 371, 1124
XapI RAATTY 3 cut(s) 623, 981, 1060
XceI RCATGY 1 cut(s) 553
XspI CTAG 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.