Rh3AG089200

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001553
Physical Location & Seq
Reverse (-)
7210093 .. 7235584
25492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG089200.1

Sequence Viewer

Length: 1458 bp
ATGTTGAAGTTCTTGTGGGTTTGCATTGTAATAGCAACTTTTTTCAGCAACTGGGTTCATGGGTTTGTTCAGTACAGCCATGAAGGAGATCCCAAGACTGAAATCATTAATAACACTCTGATGGTATCACTAACCCTCATTCAAGGAGCTGATGCCAAAGGAGCAGTCTGCTTGGATGGAACATTACCTGCTTATTATTTTCATCGGGGACACGGGTCAGGGAAAAAGAGTTGGCTCGTTCACTTTCAGGGAGGAGGATGGTGTGGCAACATCACGGATTGTGTTGCTCGCAAGAAAACGCAGCTCGGATCATCAAATTTCATGCTACCAGAGACAGGATTCACCGGAATACTAAGCTATAAAGCTGAAGAAAATCCAGATTTCTTCAACTGGAATAGAGTAATGCTTCCCTCTTGTGACGGTGCTTCTTTTTCTGGGGACAGTGAAAATAAGGAAGCACAACTGCAATTTAGAGGACATCGCATTTGGTTGGCTGAAATGGAAAAATTGATGTCATTGGGCATGCGCCACGCCAAGAAGGCTCTTATTTCTGGTTGCTCTGCTGGAGGTCTGACATCTATTTTGCACTGTGATAAGTTCCGGGGATTATTTCGAAGAAATACTGAAGTGAAGTGCCTCAGTGATGCTGGATTTTTCCTTGACTCGGTTGACATATCTGGTGCACCAACATTCAGGAATTTTTTCAGTAATGTTGTAAGCTTGCAGGGGGTGAAAGAAAATCTGCCACACTTTTGTACTAGTCGCCTCGATCCTACTTCGTGCTTCTTCCCTCAGAACTTACTTGCCGGCATTAAAACCCCGCTGTTTATACTTAACTCAGCATATGATTCTTTTCAGTTCCAATTCAGCTTAGTTCCAGCATCAGCTGATCCCAACGGCTTATGGAACAGTTGCAAATTAAACGTTACCAACTGTTCCCCAACTCAGTTGCATATTCTGCAAGGATTCAGGAGTCAAATGCTACAGGCACTAAAGCCATTCTCAAAGTCTAACCAAAATGGGATGTTTATAGATTCATGCTATGCACACTGTCAGTCTCAGTTTCAGCTAACGTGGTATGCTGCCAATTCTACAATCATTGGAAACAAGACAATCTCACAGTCCGTCGGAGATTGGTATTTCGACCGTGCAGAAGTTAAGGAGATCATTGCTCTGTCTCCACGGTGTGGTCGATGGTTTGGTCCGGCACTTCGACAACCCCCAAGCCTACAAGTACTTTGTCAAGAGCTGCCACATCATCGTTGGGACGGTGACGTCAGCACCCTCCGCGAGGTCCAGGTCATCTCTGGGTTTCCGACCAACAGCAGCACGGAGAGGCTGGACATGTTGGACGAGGAGAGCCATGTCATCAACTTCAGCATGATTGCAAAGGGGCTGAATATGTGTTCATCAATGGAGAAGGGTCGACATGGGGCCAATGGGGCCGATCAACCCTAA

Protein Analysis

485

Amino Acids

53.99

Weight (kDa)

7.85

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 38 - 389 1.7e-153 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1274
AatII GACGTC 1 cut(s) 1278
Acc36I ACCTGC 1 cut(s) 196
AccB7I CCANNNNNTGG 1 cut(s) 1187
AccI GTMKAC 1 cut(s) 1426
AccII CGCG 1 cut(s) 1290
AciI CCGC 2 cut(s) 821, 1288
AclI AACGTT 1 cut(s) 924
AclWI GGATC 4 cut(s) 83, 316, 764, 884
AcsI RAATTY 2 cut(s) 316, 697
AcuI CTGAAG 3 cut(s) 387, 645, 1360
AcyI GRCGYC 1 cut(s) 1275
AdeI CACNNNGTG 1 cut(s) 1187
AfaI GTAC 3 cut(s) 74, 757, 1236
AfiI CCNNNNNNNGG 4 cut(s) 335, 664, 1187, 1291
AflIII ACRYGT 1 cut(s) 1344
AgsI TTSAA 3 cut(s) 7, 143, 388
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 1 cut(s) 1296
AluBI AGCT 9 cut(s) 149, 304, 357, 365, 720, 870, 887, 1069, 1249
AluI AGCT 9 cut(s) 149, 304, 357, 365, 720, 870, 887, 1069, 1249
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 3 cut(s) 326, 1062, 1182
Alw44I GTGCAC 1 cut(s) 681
AlwI GGATC 4 cut(s) 83, 316, 764, 884
AlwNI CAGNNNCTG 1 cut(s) 51
AoxI GGCC 2 cut(s) 1434, 1443
ApaLI GTGCAC 1 cut(s) 681
ApeKI GCWGC 4 cut(s) 301, 1082, 1249, 1326
ApoI RAATTY 2 cut(s) 316, 697
ArsI GACNNNNNNTTYG 6 cut(s) 150, 182, 468, 500, 565, 597
AseI ATTAAT 1 cut(s) 108
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 4 cut(s) 1202, 1294, 1434, 1443
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 3 cut(s) 334, 742, 1283
AsuII TTCGAA 1 cut(s) 613
AvaII GGWCC 2 cut(s) 1202, 1294
BaeGI GKGCMC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 4 cut(s) 313, 1069, 1236, 1338
BccI CCATC 4 cut(s) 115, 170, 252, 1188
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 1 cut(s) 1298
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 3 cut(s) 326, 1062, 1182
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 1 cut(s) 759
BfmI CTRYAG 1 cut(s) 983
BfuAI ACCTGC 1 cut(s) 196
BglI GCCNNNNNGGC 2 cut(s) 539, 1442
BisI GCNGC 4 cut(s) 302, 1083, 1250, 1327
BlsI GCNGC 4 cut(s) 303, 1084, 1251, 1328
BmcAI AGTACT 1 cut(s) 1236
Bme1390I CCNGG 2 cut(s) 602, 1298
Bme18I GGWCC 2 cut(s) 1202, 1294
BmgT120I GGNCC 4 cut(s) 1202, 1294, 1434, 1443
BmiI GGNNCC 2 cut(s) 1435, 1444
BmrFI CCNGG 2 cut(s) 602, 1298
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 142, 634, 890
BmuI ACTGGG 1 cut(s) 61
BoxI GACNNNNGTC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 1275
BsaJI CCNNGG 2 cut(s) 601, 1181
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 664, 1187, 1291
Bse118I RCCGGY 1 cut(s) 806
Bse1I ACTGG 2 cut(s) 56, 395
Bse3DI GCAATG 1 cut(s) 1167
BseBI CCWGG 1 cut(s) 1298
BseDI CCNNGG 2 cut(s) 601, 1181
BseGI GGATG 3 cut(s) 181, 263, 1029
BseLI CCNNNNNNNGG 4 cut(s) 335, 664, 1187, 1291
BseMI GCAATG 1 cut(s) 1167
BseMII CTCAG 5 cut(s) 652, 806, 852, 959, 1073
BseNI ACTGG 2 cut(s) 56, 395
BseRI GAGGAG 2 cut(s) 267, 1370
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 4 cut(s) 313, 1069, 1236, 1338
BsgI GTGCAG 1 cut(s) 1170
Bsh1236I CGCG 1 cut(s) 1290
Bsh1285I CGRYCG 1 cut(s) 1147
BshFI GGCC 2 cut(s) 1436, 1445
BsiEI CGRYCG 1 cut(s) 1147
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 4 cut(s) 345, 601, 807, 1205
BslFI GGGAC 3 cut(s) 222, 452, 1280
BslI CCNNNNNNNGG 4 cut(s) 335, 664, 1187, 1291
BsmAI GTCTC 3 cut(s) 326, 1062, 1182
BsmFI GGGAC 3 cut(s) 222, 452, 1280
BsnI GGCC 2 cut(s) 1436, 1445
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 685
Bsp143I GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
BspACI CCGC 2 cut(s) 821, 1288
BspANI GGCC 2 cut(s) 1436, 1445
BspCNI CTCAG 5 cut(s) 651, 805, 851, 958, 1072
BspFNI CGCG 1 cut(s) 1290
BspLI GGNNCC 2 cut(s) 1435, 1444
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 4 cut(s) 83, 316, 764, 884
BspT104I TTCGAA 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 1167
BsrFI RCCGGY 1 cut(s) 806
BsrI ACTGG 2 cut(s) 56, 395
BssAI RCCGGY 1 cut(s) 806
BssECI CCNNGG 2 cut(s) 601, 1181
BssMI GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
BssNI GRCGYC 1 cut(s) 1275
Bst2UI CCWGG 1 cut(s) 1298
BstACI GRCGYC 1 cut(s) 1275
BstAPI GCANNNNNTGC 1 cut(s) 958
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 4 cut(s) 289, 524, 722, 808
BstDEI CTNAG 7 cut(s) 353, 638, 792, 838, 871, 945, 1059
BstDSI CCRYGG 1 cut(s) 1181
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 3 cut(s) 181, 263, 1029
BstFNI CGCG 1 cut(s) 1290
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 6 cut(s) 91, 311, 772, 892, 1167, 1450
BstMAI GTCTC 3 cut(s) 326, 1062, 1182
BstMBI GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
BstMCI CGRYCG 1 cut(s) 1147
BstMWI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1287, 1442
BstNI CCWGG 1 cut(s) 1298
BstNSI RCATGY 2 cut(s) 526, 1348
BstPAI GACNNNNGTC 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 600, 1296
BstSFI CTRYAG 1 cut(s) 983
BstSLI GKGCMC 1 cut(s) 685
BstUI CGCG 1 cut(s) 1290
BstV1I GCAGC 4 cut(s) 313, 1069, 1236, 1338
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 2 cut(s) 1436, 1445
BtgI CCRYGG 1 cut(s) 1181
BtgZI GCGATG 1 cut(s) 464
BtsCI GGATG 3 cut(s) 181, 263, 1029
BtsIMutI CAGTG 4 cut(s) 448, 586, 646, 1048
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 4 cut(s) 289, 524, 722, 808
CaiI CAGNNNCTG 1 cut(s) 51
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 806
Cfr13I GGNCC 4 cut(s) 1202, 1294, 1434, 1443
Csp6I GTAC 3 cut(s) 73, 756, 1235
CviAII CATG 9 cut(s) 59, 80, 322, 523, 1038, 1345, 1364, 1381, 1430
CviQI GTAC 3 cut(s) 73, 756, 1235
DdeI CTNAG 7 cut(s) 353, 638, 792, 838, 871, 945, 1059
DpnI GATC 6 cut(s) 90, 310, 771, 891, 1166, 1449
DpnII GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
DraIII CACNNNGTG 1 cut(s) 1187
DrdI GACNNNNNNGTC 1 cut(s) 1274
DseDI GACNNNNNNGTC 1 cut(s) 1274
Eco47I GGWCC 2 cut(s) 1202, 1294
Eco57I CTGAAG 3 cut(s) 387, 645, 1360
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoRII CCWGG 1 cut(s) 1296
FaeI CATG 9 cut(s) 62, 83, 325, 526, 1041, 1348, 1367, 1384, 1433
FaqI GGGAC 3 cut(s) 222, 452, 1280
FatI CATG 9 cut(s) 58, 79, 321, 522, 1037, 1344, 1363, 1380, 1429
FauI CCCGC 1 cut(s) 828
FauNDI CATATG 1 cut(s) 844
FblI GTMKAC 1 cut(s) 1426
Fnu4HI GCNGC 4 cut(s) 302, 1083, 1250, 1327
FokI GGATG 3 cut(s) 188, 270, 1036
Fsp4HI GCNGC 4 cut(s) 302, 1083, 1250, 1327
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 527
GluI GCNGC 4 cut(s) 302, 1083, 1250, 1327
GsuI CTGGAG 1 cut(s) 585
HaeIII GGCC 2 cut(s) 1436, 1445
HapII CCGG 4 cut(s) 345, 601, 807, 1205
HhaI GCGC 1 cut(s) 528
Hin1I GRCGYC 1 cut(s) 1275
Hin1II CATG 9 cut(s) 62, 83, 325, 526, 1041, 1348, 1367, 1384, 1433
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 2 cut(s) 670, 1427
HindII GTYRAC 2 cut(s) 670, 1427
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 6 cut(s) 339, 662, 848, 966, 973, 1034
HpaII CCGG 4 cut(s) 345, 601, 807, 1205
HphI GGTGA 3 cut(s) 334, 742, 1283
Hpy166II GTNNAC 4 cut(s) 241, 670, 683, 1427
Hpy188I TCNGA 6 cut(s) 120, 308, 573, 795, 1130, 1317
Hpy188III TCNNGA 4 cut(s) 377, 694, 970, 1244
Hpy8I GTNNAC 4 cut(s) 241, 670, 683, 1427
Hpy99I CGWCG 1 cut(s) 1130
HpyAV CCTTC 3 cut(s) 77, 532, 1414
HpyCH4IV ACGT 3 cut(s) 924, 1073, 1275
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1287, 1442
HpyF3I CTNAG 7 cut(s) 353, 638, 792, 838, 871, 945, 1059
HpySE526I ACGT 3 cut(s) 924, 1073, 1275
Hsp92I GRCGYC 1 cut(s) 1275
Hsp92II CATG 9 cut(s) 62, 83, 325, 526, 1041, 1348, 1367, 1384, 1433
HspAI GCGC 1 cut(s) 526
KroI GCCGGC 1 cut(s) 806
KroNI GCCGGC 1 cut(s) 808
Kzo9I GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
LmnI GCTCC 2 cut(s) 146, 161
Lsp1109I GCAGC 4 cut(s) 313, 1069, 1236, 1338
LweI GCATC 3 cut(s) 142, 634, 890
MaeI CTAG 1 cut(s) 759
MaeII ACGT 3 cut(s) 924, 1073, 1275
MaeIII GTNAC 3 cut(s) 416, 925, 1271
MalI GATC 6 cut(s) 90, 310, 771, 891, 1166, 1449
MboI GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
MboII GAAGA 4 cut(s) 376, 380, 627, 778
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
MlyI GAGTC 2 cut(s) 656, 982
MmeI TCCRAC 3 cut(s) 1108, 1329, 1340
MroNI GCCGGC 1 cut(s) 806
MroXI GAANNNNTTC 1 cut(s) 701
MseI TTAA 5 cut(s) 108, 813, 834, 920, 1158
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 823, 887
MspI CCGG 4 cut(s) 345, 601, 807, 1205
MspR9I CCNGG 2 cut(s) 602, 1298
MvaI CCWGG 1 cut(s) 1298
MvnI CGCG 1 cut(s) 1290
MwoI GCNNNNNNNGC 5 cut(s) 161, 539, 958, 1287, 1442
NaeI GCCGGC 1 cut(s) 808
NciI CCSGG 1 cut(s) 602
NdeI CATATG 1 cut(s) 844
NdeII GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
NgoMIV GCCGGC 1 cut(s) 806
NlaIII CATG 9 cut(s) 62, 83, 325, 526, 1041, 1348, 1367, 1384, 1433
NlaIV GGNNCC 2 cut(s) 1435, 1444
NmuCI GTSAC 2 cut(s) 416, 1271
NspI RCATGY 2 cut(s) 526, 1348
NspV TTCGAA 1 cut(s) 613
PaeI GCATGC 1 cut(s) 526
PciI ACATGT 1 cut(s) 1344
PcsI WCGNNNNNNNCGW 1 cut(s) 1189
PdiI GCCGGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 4 cut(s) 339, 848, 966, 1034
PflMI CCANNNNNTGG 1 cut(s) 1187
PkrI GCNGC 4 cut(s) 303, 1084, 1251, 1328
PleI GAGTC 2 cut(s) 656, 981
PpsI GAGTC 2 cut(s) 656, 981
PscI ACATGT 1 cut(s) 1344
PshAI GACNNNNGTC 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 108
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 1296
PspGI CCWGG 1 cut(s) 1296
PspN4I GGNNCC 2 cut(s) 1435, 1444
PspPI GGNCC 4 cut(s) 1202, 1294, 1434, 1443
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 3 cut(s) 74, 757, 1236
RsaNI GTAC 3 cut(s) 73, 756, 1235
RseI CAYNNNNRTG 1 cut(s) 119
SalI GTCGAC 1 cut(s) 1425
SaqAI TTAA 5 cut(s) 108, 813, 834, 920, 1158
SatI GCNGC 4 cut(s) 302, 1083, 1250, 1327
Sau3AI GATC 6 cut(s) 88, 308, 769, 889, 1164, 1447
Sau96I GGNCC 4 cut(s) 1202, 1294, 1434, 1443
ScaI AGTACT 1 cut(s) 1236
SchI GAGTC 2 cut(s) 656, 982
ScrFI CCNGG 2 cut(s) 602, 1298
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 3 cut(s) 142, 634, 890
SfcI CTRYAG 1 cut(s) 983
SfiI GGCCNNNNNGGCC 1 cut(s) 1442
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 2 cut(s) 1202, 1294
SmiMI CAYNNNNRTG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 758
SphI GCATGC 1 cut(s) 526
Sse9I AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
SsiI CCGC 2 cut(s) 821, 1288
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 2 cut(s) 600, 1296
TaiI ACGT 3 cut(s) 927, 1076, 1278
TaqI TCGA 6 cut(s) 613, 768, 1143, 1192, 1213, 1426
TasI AATT 7 cut(s) 316, 467, 506, 697, 863, 917, 1087
TatI WGTACW 3 cut(s) 72, 755, 1234
TfiI GAWTC 4 cut(s) 339, 848, 966, 1034
Tru1I TTAA 5 cut(s) 108, 813, 834, 920, 1158
Tru9I TTAA 5 cut(s) 108, 813, 834, 920, 1158
TscAI CASTG 4 cut(s) 448, 593, 646, 1055
TseFI GTSAC 2 cut(s) 416, 1271
TseI GCWGC 4 cut(s) 301, 1082, 1249, 1326
Tsp45I GTSAC 2 cut(s) 416, 1271
TspDTI ATGAA 6 cut(s) 47, 96, 191, 310, 1026, 1398
TspGWI ACGGA 3 cut(s) 290, 1114, 1346
TspRI CASTG 4 cut(s) 448, 593, 646, 1055
Van91I CCANNNNNTGG 1 cut(s) 1187
VneI GTGCAC 1 cut(s) 681
VpaK11BI GGWCC 2 cut(s) 1202, 1294
VspI ATTAAT 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 2 cut(s) 316, 697
XceI RCATGY 2 cut(s) 526, 1348
XcmI CCANNNNNNNNNTGG 2 cut(s) 1260, 1304
XmiI GTMKAC 1 cut(s) 1426
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 1 cut(s) 759
ZraI GACGTC 1 cut(s) 1276
ZrmI AGTACT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.