MD12G1197900.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001553
Physical Location & Seq
Forward (+)
27904395 .. 27913221
8827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1197900.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGAAGCTTTTCTGGGTTAGCATTGTGATTTCACTTGCTTTCAGCAAATGGGCTGCTGGGTTTATGGAGTACGGCTTCAATGAAACAGAGATGGCTTTGTTGGAGGGCTATGGAGAATCAAAGGTTGGAGCTGACTTGCTGATGGTTGGACTGACCATCATTGGAGGAGCTGGGGCCAAAGGAGCAGTCTGCTTGGATGGAACCTTACCGGGGTATCATTTGCATCGCGGATACGGGTCAGGAGCAAATAGTTGGCTCATTCAATTGGAGGGGGGAGGATGGTGTAACAACATTAGAACTTGTGTTTATCGAAAAACAACAAGGCGGGGATCATCAAAATACATGGAAAAACAGTTAGTATTCAGTGGAATACTAAGCAATAAAGCTGAAGAAAATCCTGATTTTTTCAACTGGAACAGAGTCAAGGTTCGTTATTGCGATGGTGCTTCTTTTTCTGGGGATAGTGAAGATAAGGCCAAAGAACTGCAATTTAGAGGAGAGCGTATATGGTTGGCTGCAATGGAAGACTTGAAATCAAAGGGCATGAGCTACGCCAAGCAGGCTCTTCTTTCGGGTTGCTCTGCCGGTGGTGTGGCAGCTATTTTGCACTGTGATAAGTTCCGGGGTTTAATGCCTGGAACTACGAAAGTGAAGTGCCTGAGTGATGCTGGATTGTTTCTTGATGCGAATGACGTTTCTGGTGGACGCACACTCAGGAATTTATATAGTGGCATTGTCGGCTTGCAGGGGGTGAAACCAAATCTGCCACAATATTGCACTAATCACCTTGACCCAACTTCGTGCTTCTTCCCTCAGAACTTGATCTCCAGCATCAAAACCCCACTGTTTATACTCAATGCAGCATATGATTCGTGGCAGATCCAGACCAGTTTAGCTCCACCGGCAGCAGATCCTGCCGGCTACTGGCGCGATTGCAGATTAAACCATGAAAAATGTACTCCATCACAGATCAATTTTCTTCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAGTTCTCTATGTCTGAACAAAACGGATTGTTTATAAATTCGTGTTTTGCGCACTGCCAATCCGAGAGGCAGGATACATGGTTTGCTGATGATTCTCCAGTTATTGGAAAGAAGGCAATCGCTATAGCAGTTGGAGATTGGTATTTCGACCGAACAAGCGTGAAGATCATAGATTGTCCGTACCCATGTGACAATTCTTGCCACAATCTGAATTTCGAAAAAGCTTAG

Protein Analysis

415

Amino Acids

45.69

Weight (kDa)

8.07

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 47 - 407 1.5e-173 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
Acc16I TGCGCA 1 cut(s) 1068
AccB7I CCANNNNNTGG 1 cut(s) 1121
AccII CGCG 2 cut(s) 228, 930
AciI CCGC 2 cut(s) 228, 325
AclWI GGATC 3 cut(s) 337, 874, 905
AcsI RAATTY 3 cut(s) 718, 1054, 1228
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 71, 958, 1199
AfiI CCNNNNNNNGG 3 cut(s) 210, 924, 1121
AgsI TTSAA 5 cut(s) 79, 263, 409, 532, 983
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AloI GAACNNNNNNTCC 2 cut(s) 809, 841
AluBI AGCT 8 cut(s) 7, 131, 170, 386, 549, 599, 896, 1241
AluI AGCT 8 cut(s) 7, 131, 170, 386, 549, 599, 896, 1241
AlwI GGATC 3 cut(s) 337, 874, 905
AlwNI CAGNNNCTG 1 cut(s) 914
AoxI GGCC 2 cut(s) 174, 474
ApeKI GCWGC 5 cut(s) 53, 515, 596, 860, 905
ApoI RAATTY 3 cut(s) 718, 1054, 1228
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 2 cut(s) 930, 1069
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 2 cut(s) 210, 623
AsuHPI GGTGA 2 cut(s) 763, 776
AsuII TTCGAA 1 cut(s) 1233
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 5 cut(s) 40, 502, 608, 872, 917
BccI CCATC 7 cut(s) 85, 136, 164, 191, 273, 434, 970
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 852, 886
BciT130I CCWGG 1 cut(s) 636
BciVI GTATCC 2 cut(s) 224, 1084
BcnI CCSGG 2 cut(s) 210, 623
BfmI CTRYAG 1 cut(s) 1140
BfuI GTATCC 2 cut(s) 224, 1084
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 5 cut(s) 54, 516, 597, 861, 906
BlsI GCNGC 5 cut(s) 55, 517, 598, 862, 907
Bme1390I CCNGG 3 cut(s) 210, 623, 636
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 175, 202
BmrFI CCNGG 3 cut(s) 210, 623, 636
BmsI GCATC 4 cut(s) 232, 655, 673, 840
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 2 cut(s) 811, 1098
Bpu14I TTCGAA 1 cut(s) 1233
BpuMI CCSGG 2 cut(s) 210, 623
BsaJI CCNNGG 2 cut(s) 209, 622
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 924, 1121
Bse118I RCCGGY 3 cut(s) 584, 901, 917
Bse1I ACTGG 4 cut(s) 416, 888, 929, 1115
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 209, 622
BseGI GGATG 2 cut(s) 202, 284
BseLI CCNNNNNNNGG 3 cut(s) 210, 924, 1121
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 3 cut(s) 650, 727, 827
BseNI ACTGG 4 cut(s) 416, 888, 929, 1115
BseRI GAGGAG 2 cut(s) 180, 510
BseXI GCAGC 5 cut(s) 40, 502, 608, 872, 917
BseYI CCCAGC 2 cut(s) 56, 170
Bsh1236I CGCG 2 cut(s) 228, 930
Bsh1285I CGRYCG 1 cut(s) 1168
BshFI GGCC 2 cut(s) 176, 476
BsiEI CGRYCG 1 cut(s) 1168
BsiSI CCGG 5 cut(s) 209, 585, 622, 902, 918
BslI CCNNNNNNNGG 3 cut(s) 210, 924, 1121
BsmI GAATGC 1 cut(s) 1012
BsnI GGCC 2 cut(s) 176, 476
Bsp119I TTCGAA 1 cut(s) 1233
Bsp143I GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
BspACI CCGC 2 cut(s) 228, 325
BspANI GGCC 2 cut(s) 176, 476
BspCNI CTCAG 3 cut(s) 651, 726, 826
BspFNI CGCG 2 cut(s) 228, 930
BspLI GGNNCC 2 cut(s) 175, 202
BspPI GGATC 3 cut(s) 337, 874, 905
BspQI GCTCTTC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 1233
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 3 cut(s) 584, 901, 917
BsrI ACTGG 4 cut(s) 416, 888, 929, 1115
BssAI RCCGGY 3 cut(s) 584, 901, 917
BssECI CCNNGG 2 cut(s) 209, 622
BssMI GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 4 cut(s) 354, 611, 846, 1020
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 914
BstBI TTCGAA 1 cut(s) 1233
BstC8I GCNNGC 3 cut(s) 561, 743, 919
BstDEI CTNAG 5 cut(s) 374, 659, 713, 813, 1242
BstF5I GGATG 2 cut(s) 202, 284
BstFNI CGCG 2 cut(s) 228, 930
BstHHI GCGC 2 cut(s) 930, 1069
BstKTI GATC 6 cut(s) 332, 825, 882, 913, 972, 1185
BstMBI GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
BstMCI CGRYCG 1 cut(s) 1168
BstMWI GCNNNNNNNGC 6 cut(s) 182, 560, 738, 902, 914, 927
BstNI CCWGG 1 cut(s) 636
BstSCI CCNGG 3 cut(s) 208, 621, 634
BstSFI CTRYAG 1 cut(s) 1140
BstUI CGCG 2 cut(s) 228, 930
BstV1I GCAGC 5 cut(s) 40, 502, 608, 872, 917
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 2 cut(s) 879, 910
BstYI RGATCY 2 cut(s) 879, 910
BsuI GTATCC 2 cut(s) 224, 1084
BsuRI GGCC 2 cut(s) 176, 476
BtgZI GCGATG 2 cut(s) 209, 453
BtsCI GGATG 2 cut(s) 202, 284
BtsI GCAGTG 1 cut(s) 1069
BtsIMutI CAGTG 4 cut(s) 370, 607, 842, 1069
Cac8I GCNNGC 3 cut(s) 561, 743, 919
CaiI CAGNNNCTG 1 cut(s) 914
CfoI GCGC 2 cut(s) 930, 1069
Cfr10I RCCGGY 3 cut(s) 584, 901, 917
Cfr13I GGNCC 1 cut(s) 174
CseI GACGC 1 cut(s) 714
Csp6I GTAC 3 cut(s) 70, 957, 1198
CviAII CATG 5 cut(s) 343, 544, 947, 1095, 1203
CviQI GTAC 3 cut(s) 70, 957, 1198
DdeI CTNAG 5 cut(s) 374, 659, 713, 813, 1242
DpnI GATC 6 cut(s) 331, 824, 881, 912, 971, 1184
DpnII GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco57I CTGAAG 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 634
FaeI CATG 5 cut(s) 346, 547, 950, 1098, 1206
FatI CATG 5 cut(s) 342, 543, 946, 1094, 1202
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 5 cut(s) 54, 516, 597, 861, 906
FokI GGATG 2 cut(s) 209, 291
Fsp4HI GCNGC 5 cut(s) 54, 516, 597, 861, 906
FspI TGCGCA 1 cut(s) 1068
GlaI GCGC 2 cut(s) 929, 1068
GluI GCNGC 5 cut(s) 54, 516, 597, 861, 906
GsaI CCCAGC 2 cut(s) 60, 174
GsuI CTGGAG 2 cut(s) 811, 1098
HaeIII GGCC 2 cut(s) 176, 476
HapII CCGG 5 cut(s) 209, 585, 622, 902, 918
HgaI GACGC 1 cut(s) 714
HhaI GCGC 2 cut(s) 930, 1069
Hin1II CATG 5 cut(s) 346, 547, 950, 1098, 1206
Hin6I GCGC 2 cut(s) 928, 1067
HinP1I GCGC 2 cut(s) 928, 1067
HindIII AAGCTT 2 cut(s) 5, 1239
HinfI GANTC 6 cut(s) 116, 420, 869, 987, 994, 1109
HpaII CCGG 5 cut(s) 209, 585, 622, 902, 918
HphI GGTGA 2 cut(s) 763, 776
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 4 cut(s) 816, 1033, 1081, 1227
Hpy188III TCNNGA 6 cut(s) 240, 398, 680, 715, 883, 991
Hpy8I GTNNAC 1 cut(s) 704
HpyAV CCTTC 1 cut(s) 1123
HpyCH4III ACNGT 4 cut(s) 354, 611, 846, 1020
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 9 cut(s) 223, 487, 518, 607, 745, 777, 860, 936, 1010
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 560, 738, 902, 914, 927
HpyF3I CTNAG 5 cut(s) 374, 659, 713, 813, 1242
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 5 cut(s) 346, 547, 950, 1098, 1206
HspAI GCGC 2 cut(s) 928, 1067
KroI GCCGGC 1 cut(s) 917
KroNI GCCGGC 1 cut(s) 919
Kzo9I GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 5 cut(s) 128, 167, 182, 242, 901
Lsp1109I GCAGC 5 cut(s) 40, 502, 608, 872, 917
LweI GCATC 4 cut(s) 232, 655, 673, 840
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 2 cut(s) 284, 1205
MalI GATC 6 cut(s) 331, 824, 881, 912, 971, 1184
MboI GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
MboII GAAGA 7 cut(s) 401, 479, 536, 557, 799, 971, 1192
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 879, 910
MluCI AATT 7 cut(s) 263, 488, 718, 973, 1054, 1210, 1228
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 4 cut(s) 81, 106, 127, 1129
MnlI CCTC 7 cut(s) 97, 158, 262, 269, 488, 822, 1077
MroNI GCCGGC 1 cut(s) 917
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 2 cut(s) 629, 941
MspI CCGG 5 cut(s) 209, 585, 622, 902, 918
MspR9I CCNGG 3 cut(s) 210, 623, 636
MunI CAATTG 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 1012
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 2 cut(s) 228, 930
MwoI GCNNNNNNNGC 6 cut(s) 182, 560, 738, 902, 914, 927
NaeI GCCGGC 1 cut(s) 919
NciI CCSGG 2 cut(s) 210, 623
NdeI CATATG 1 cut(s) 865
NdeII GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
NgoMIV GCCGGC 1 cut(s) 917
NlaIII CATG 5 cut(s) 346, 547, 950, 1098, 1206
NlaIV GGNNCC 2 cut(s) 175, 202
NmuCI GTSAC 1 cut(s) 1205
NsbI TGCGCA 1 cut(s) 1068
NspV TTCGAA 1 cut(s) 1233
PciSI GCTCTTC 1 cut(s) 570
PctI GAATGC 1 cut(s) 1012
PdiI GCCGGC 1 cut(s) 919
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 5 cut(s) 116, 869, 987, 994, 1109
PflMI CCANNNNNTGG 1 cut(s) 1121
PkrI GCNGC 5 cut(s) 55, 517, 598, 862, 907
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
PsiI TTATAA 1 cut(s) 1052
Psp6I CCWGG 1 cut(s) 634
PspFI CCCAGC 2 cut(s) 56, 170
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 2 cut(s) 175, 202
PspPI GGNCC 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 2 cut(s) 879, 910
RsaI GTAC 3 cut(s) 71, 958, 1199
RsaNI GTAC 3 cut(s) 70, 957, 1198
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 2 cut(s) 629, 941
SatI GCNGC 5 cut(s) 54, 516, 597, 861, 906
Sau3AI GATC 6 cut(s) 329, 822, 879, 910, 969, 1182
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 3 cut(s) 210, 623, 636
SfaNI GCATC 4 cut(s) 232, 655, 673, 840
SfcI CTRYAG 1 cut(s) 1140
SfuI TTCGAA 1 cut(s) 1233
Sse9I AATT 7 cut(s) 263, 488, 718, 973, 1054, 1210, 1228
SsiI CCGC 2 cut(s) 228, 325
SspI AATATT 1 cut(s) 773
StyD4I CCNGG 3 cut(s) 208, 621, 634
TaaI ACNGT 4 cut(s) 354, 611, 846, 1020
TaiI ACGT 1 cut(s) 696
TaqI TCGA 3 cut(s) 310, 1164, 1233
TaqII GACCGA 1 cut(s) 1182
TasI AATT 7 cut(s) 263, 488, 718, 973, 1054, 1210, 1228
TatI WGTACW 1 cut(s) 956
TfiI GAWTC 5 cut(s) 116, 869, 987, 994, 1109
Tru1I TTAA 2 cut(s) 629, 941
Tru9I TTAA 2 cut(s) 629, 941
TscAI CASTG 4 cut(s) 370, 614, 849, 1076
TseFI GTSAC 1 cut(s) 1205
TseI GCWGC 5 cut(s) 53, 515, 596, 860, 905
Tsp45I GTSAC 1 cut(s) 1205
TspDTI ATGAA 3 cut(s) 17, 96, 963
TspGWI ACGGA 2 cut(s) 1056, 1185
TspRI CASTG 4 cut(s) 370, 614, 849, 1076
Van91I CCANNNNNTGG 1 cut(s) 1121
XapI RAATTY 3 cut(s) 718, 1054, 1228
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.