MD04G1184700.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001553
Physical Location & Seq
Forward (+)
27584295 .. 27587144
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1184700.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGTGAAGTTCTTGTGGGTTGGCATTGTAACAGCACTTGTTTTCAGAATTTGGGTTGATGGGTTTGTGGAAGAGAAGAATTTTGATGAGACGGTGCCGTCTTTGTTGGAGACATATGGAAGCTCCGAGGCTGCAAATGACATACTCATGGTAGGGCTAACACTCGTCAAAGGAGCTGCTGCCAAAGGAGCAGTCTGCTTGGATGGAACAGTACCTGCTTACCATTTGCATCGTGGATATGGATCGGGGGCGAACAGTTGGCTCATTCACCTTCAGGGAGGAGCATGGTGTAACGACACCAAAAGTTGTTCTAACCGCAAGCTTTCACCTTATGGATCATCAGCGCACATGGAACCAGAGATAGCATTTTCTGGAATACTAAGCAATCACTCTGAAGAAAATCCCGAGTTTTTCAACTGGAATAGAGTAGAGCTCCGGTATTGTGACGGTGCTTCATTTTCTGGGGACGGCGAAAATCAGGCAGCAAAACTGAAATTTAGAGGACAGCGTATATGGTTAGCTGCAATGGACGATTTGATGTCAAAGGGAATGCGCGACGCGAATCATGCTCTTCTTTCTGGTTGCTCTGCCGGCGGTCTGTCATCAATTTTGCACTGTGATGAATTTCGGGGCTTGTTTCCCACAACTACAAAAGTGAAGTGCCTGAGTGATGCTGGATTTTTTCTTGACGCGGTTGATATTTCTGGTGAACGCACAATGAGGGATTTTTATAGTGGTGTAGTAAGCTTGCAGGGGGTGCAAGAAAATCTGCCGCGATTCTGTACTAGTCACCTCGATCCTACTTCGTGCTTCTTCCCCGAGAACTTAATTGTGAACATTACAACCCCGCTATTTATACTCAATTCTGCATATGATTCATGGCAGATCAATAACAGTTTAGCTCCACCATCAGCAGATCCGCACGGTGAGTGGAAGAGTTGCAGATATAACCTTGCAAGTTGTTCCGCATCACAGATGCAAATTCTTCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAATTCTCAATGCCTAATCAAAATGGGATGTTCATAAATTCGTGTTTGGTGCACTGTCAAACGGAGAAAAGTGCATGGTATGCTAATGAATCTCCAGCTCTTGGAAACAAGACAATTGCAGAAGCTGTTGGAGATTGGTATTTTGATAGATCAGAGGTTAAGGTTGTTGATGAAACTTGCCGCCATCTGGCCCTCGAATGA

Protein Analysis

408

Amino Acids

44.9

Weight (kDa)

5.41

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 48 - 401 7.1e-161 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 223
AccB1I GGYRCC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 1124
AccII CGCG 4 cut(s) 555, 560, 692, 775
AciI CCGC 8 cut(s) 316, 594, 692, 773, 848, 920, 966, 1204
AclWI GGATC 4 cut(s) 250, 343, 791, 911
AcsI RAATTY 6 cut(s) 48, 79, 494, 623, 981, 1060
AcuI CTGAAG 2 cut(s) 257, 414
AfaI GTAC 2 cut(s) 213, 784
AfiI CCNNNNNNNGG 2 cut(s) 1124, 1210
AgsI TTSAA 2 cut(s) 415, 989
AhlI ACTAGT 1 cut(s) 785
AluBI AGCT 9 cut(s) 123, 176, 322, 433, 521, 747, 902, 1121, 1148
AluI AGCT 9 cut(s) 123, 176, 322, 433, 521, 747, 902, 1121, 1148
Alw21I GWGCWC 2 cut(s) 435, 1077
Alw26I GTCTC 2 cut(s) 83, 104
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 4 cut(s) 250, 343, 791, 911
AlwNI CAGNNNCTG 2 cut(s) 215, 1148
Ama87I CYCGRG 2 cut(s) 404, 818
AoxI GGCC 1 cut(s) 1212
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 5 cut(s) 131, 176, 179, 482, 521
ApoI RAATTY 6 cut(s) 48, 79, 494, 623, 981, 1060
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspLEI GCGC 2 cut(s) 346, 555
AspS9I GGNCC 1 cut(s) 1213
AsuHPI GGTGA 6 cut(s) 16, 260, 318, 719, 782, 938
AvaI CYCGRG 2 cut(s) 404, 818
BaeGI GKGCMC 1 cut(s) 1077
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 435, 1077
BbvI GCAGC 5 cut(s) 118, 163, 166, 494, 508
BccI CCATC 4 cut(s) 53, 197, 916, 1215
BceAI ACGGC 2 cut(s) 82, 484
BcoDI GTCTC 2 cut(s) 83, 104
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 7 cut(s) 132, 177, 180, 483, 522, 773, 1204
BlsI GCNGC 7 cut(s) 133, 178, 181, 484, 523, 774, 1205
BmeT110I CYCGRG 2 cut(s) 404, 818
BmgT120I GGNCC 1 cut(s) 1213
BmiI GGNNCC 2 cut(s) 96, 354
BmsI GCATC 4 cut(s) 238, 661, 966, 977
BplI GAGNNNNNCTC 2 cut(s) 417, 449
BpmI CTGGAG 1 cut(s) 1101
BsaBI GATNNNNATC 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 2 cut(s) 1124, 1210
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 208, 1056
BseJI GATNNNNATC 1 cut(s) 241
BseLI CCNNNNNNNGG 2 cut(s) 1124, 1210
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 1 cut(s) 1077
BseXI GCAGC 5 cut(s) 118, 163, 166, 494, 508
Bsh1236I CGCG 4 cut(s) 555, 560, 692, 775
BshFI GGCC 1 cut(s) 1214
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 435, 1077
BsiHKCI CYCGRG 2 cut(s) 404, 818
BsiSI CCGG 2 cut(s) 436, 591
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 2 cut(s) 1124, 1210
BsmAI GTCTC 2 cut(s) 83, 104
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 2 cut(s) 555, 1018
BsnI GGCC 1 cut(s) 1214
BsoBI CYCGRG 2 cut(s) 404, 818
Bsp1286I GDGCHC 2 cut(s) 435, 1077
Bsp143I GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
BspACI CCGC 8 cut(s) 316, 594, 692, 773, 848, 920, 966, 1204
BspANI GGCC 1 cut(s) 1214
BspCNI CTCAG 1 cut(s) 657
BspFNI CGCG 4 cut(s) 555, 560, 692, 775
BspLI GGNNCC 2 cut(s) 96, 354
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 4 cut(s) 250, 343, 791, 911
BspQI GCTCTTC 1 cut(s) 576
BspT107I GGYRCC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 590
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
Bst4CI ACNGT 8 cut(s) 94, 211, 257, 449, 617, 896, 926, 1079
Bst6I CTCTTC 3 cut(s) 66, 576, 929
BstAPI GCANNNNNTGC 2 cut(s) 757, 1103
BstC8I GCNNGC 3 cut(s) 320, 592, 749
BstDEI CTNAG 2 cut(s) 380, 665
BstF5I GGATG 2 cut(s) 208, 1056
BstFNI CGCG 4 cut(s) 555, 560, 692, 775
BstHHI GCGC 2 cut(s) 346, 555
BstKTI GATC 6 cut(s) 245, 338, 799, 888, 919, 1175
BstMAI GTCTC 2 cut(s) 83, 104
BstMBI GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
BstMWI GCNNNNNNNGC 5 cut(s) 188, 566, 591, 757, 1103
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 4 cut(s) 555, 560, 692, 775
BstV1I GCAGC 5 cut(s) 118, 163, 166, 494, 508
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
BsuRI GGCC 1 cut(s) 1214
BtsCI GGATG 2 cut(s) 208, 1056
BtsIMutI CAGTG 2 cut(s) 613, 1075
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 3 cut(s) 320, 592, 749
CaiI CAGNNNCTG 2 cut(s) 215, 1148
CfoI GCGC 2 cut(s) 346, 555
Cfr10I RCCGGY 1 cut(s) 590
Cfr13I GGNCC 1 cut(s) 1213
CseI GACGC 2 cut(s) 566, 698
Csp6I GTAC 2 cut(s) 212, 783
CviAII CATG 6 cut(s) 148, 285, 349, 566, 879, 1098
CviQI GTAC 2 cut(s) 212, 783
DdeI CTNAG 2 cut(s) 380, 665
DpnI GATC 6 cut(s) 244, 337, 798, 887, 918, 1174
DpnII GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 3 cut(s) 66, 576, 929
EarI CTCTTC 3 cut(s) 66, 576, 929
Ecl136II GAGCTC 1 cut(s) 433
Eco24I GRGCYC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 257, 414
Eco88I CYCGRG 2 cut(s) 404, 818
EcoICRI GAGCTC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 6 cut(s) 151, 288, 352, 569, 882, 1101
FaqI GGGAC 1 cut(s) 479
FatI CATG 6 cut(s) 147, 284, 348, 565, 878, 1097
FauI CCCGC 1 cut(s) 855
FauNDI CATATG 2 cut(s) 115, 871
Fnu4HI GCNGC 7 cut(s) 132, 177, 180, 483, 522, 773, 1204
FokI GGATG 2 cut(s) 215, 1063
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 7 cut(s) 132, 177, 180, 483, 522, 773, 1204
FspBI CTAG 1 cut(s) 786
GlaI GCGC 2 cut(s) 345, 554
GluI GCNGC 7 cut(s) 132, 177, 180, 483, 522, 773, 1204
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 1 cut(s) 1214
HapII CCGG 2 cut(s) 436, 591
HgaI GACGC 2 cut(s) 566, 698
HhaI GCGC 2 cut(s) 346, 555
Hin1II CATG 6 cut(s) 151, 288, 352, 569, 882, 1101
Hin6I GCGC 2 cut(s) 344, 553
HinP1I GCGC 2 cut(s) 344, 553
HindIII AAGCTT 2 cut(s) 320, 745
HinfI GANTC 6 cut(s) 562, 777, 875, 993, 1000, 1112
HpaII CCGG 2 cut(s) 436, 591
HphI GGTGA 6 cut(s) 16, 260, 318, 719, 782, 938
Hpy166II GTNNAC 3 cut(s) 710, 835, 1075
Hpy188I TCNGA 4 cut(s) 47, 127, 394, 1177
Hpy188III TCNNGA 4 cut(s) 372, 404, 686, 997
Hpy8I GTNNAC 3 cut(s) 710, 835, 1075
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 8 cut(s) 94, 211, 257, 449, 617, 896, 926, 1079
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 566, 591, 757, 1103
HpyF3I CTNAG 2 cut(s) 380, 665
Hsp92II CATG 6 cut(s) 151, 288, 352, 569, 882, 1101
HspAI GCGC 2 cut(s) 344, 553
KroI GCCGGC 1 cut(s) 590
KroNI GCCGGC 1 cut(s) 592
Kzo9I GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 6 cut(s) 128, 173, 188, 281, 438, 907
Lsp1109I GCAGC 5 cut(s) 118, 163, 166, 494, 508
LweI GCATC 4 cut(s) 238, 661, 966, 977
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 4 cut(s) 28, 290, 443, 788
MalI GATC 6 cut(s) 244, 337, 798, 887, 918, 1174
MboI GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
MboII GAAGA 7 cut(s) 83, 88, 407, 563, 805, 946, 977
MfeI CAATTG 1 cut(s) 1137
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 2 cut(s) 435, 1077
MmeI TCCRAC 2 cut(s) 87, 1132
MnlI CCTC 6 cut(s) 121, 272, 494, 714, 803, 1171
MroNI GCCGGC 1 cut(s) 590
MseI TTAA 2 cut(s) 827, 1182
MslI CAYNNNNRTG 2 cut(s) 146, 618
MspI CCGG 2 cut(s) 436, 591
MunI CAATTG 1 cut(s) 1137
Mva1269I GAATGC 2 cut(s) 555, 1018
MvnI CGCG 4 cut(s) 555, 560, 692, 775
MwoI GCNNNNNNNGC 5 cut(s) 188, 566, 591, 757, 1103
NaeI GCCGGC 1 cut(s) 592
NdeI CATATG 2 cut(s) 115, 871
NdeII GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
NgoMIV GCCGGC 1 cut(s) 590
NlaIII CATG 6 cut(s) 151, 288, 352, 569, 882, 1101
NlaIV GGNNCC 2 cut(s) 96, 354
NmuCI GTSAC 2 cut(s) 443, 788
PciSI GCTCTTC 1 cut(s) 576
PctI GAATGC 2 cut(s) 555, 1018
PdiI GCCGGC 1 cut(s) 592
PfeI GAWTC 6 cut(s) 562, 777, 875, 993, 1000, 1112
PflMI CCANNNNNTGG 1 cut(s) 1124
PkrI GCNGC 7 cut(s) 133, 178, 181, 484, 523, 774, 1205
Psp124BI GAGCTC 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 96, 354
PspPI GGNCC 1 cut(s) 1213
PstNI CAGNNNCTG 2 cut(s) 215, 1148
PsuI RGATCY 1 cut(s) 916
RsaI GTAC 2 cut(s) 213, 784
RsaNI GTAC 2 cut(s) 212, 783
RseI CAYNNNNRTG 2 cut(s) 146, 618
SacI GAGCTC 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 827, 1182
SatI GCNGC 7 cut(s) 132, 177, 180, 483, 522, 773, 1204
Sau3AI GATC 6 cut(s) 242, 335, 796, 885, 916, 1172
Sau96I GGNCC 1 cut(s) 1213
SduI GDGCHC 2 cut(s) 435, 1077
SfaNI GCATC 4 cut(s) 238, 661, 966, 977
SmiMI CAYNNNNRTG 2 cut(s) 146, 618
SpeI ACTAGT 1 cut(s) 785
SsiI CCGC 8 cut(s) 316, 594, 692, 773, 848, 920, 966, 1204
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 1 cut(s) 435
TaaI ACNGT 8 cut(s) 94, 211, 257, 449, 617, 896, 926, 1079
TaqI TCGA 2 cut(s) 795, 1218
TatI WGTACW 1 cut(s) 782
TauI GCSGC 2 cut(s) 775, 1206
TfiI GAWTC 6 cut(s) 562, 777, 875, 993, 1000, 1112
Tru1I TTAA 2 cut(s) 827, 1182
Tru9I TTAA 2 cut(s) 827, 1182
TscAI CASTG 2 cut(s) 620, 1082
TseFI GTSAC 2 cut(s) 443, 788
TseI GCWGC 5 cut(s) 131, 176, 179, 482, 521
Tsp45I GTSAC 2 cut(s) 443, 788
TspDTI ATGAA 6 cut(s) 444, 636, 867, 1045, 1125, 1209
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 2 cut(s) 620, 1082
Van91I CCANNNNNTGG 1 cut(s) 1124
VneI GTGCAC 1 cut(s) 1073
XapI RAATTY 6 cut(s) 48, 79, 494, 623, 981, 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.