MD04G1184800.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001553
Physical Location & Seq
Forward (+)
27588064 .. 27591105
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1184800.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGTGAAGTTCTTGTGGATTGGCATTGTAACAGCACTTGTGTTCAGAACTTGGGTTGATGGGTTTGTGGAAGAGAAGAATCTTGATGAGACGGTGCCATCTTTGTCGGAGACCAATGGAAGCTCCCAGGCTTTTAATGACACACTACTCATGGTAGAGCTAACACTCGTCAAAGAAGCTGCAGCTAAAGGAGCAGTCTGCTTGGATGGAACAGCACCTGCTTATCATTTACATCATGGATATGGATCGGGAGCGAACAGTTGGCTCATTCACCTTCAGGGAGGAGCATGGTGTAACGACACCAAAAGTTGTTCTAACCGCACAACTTCACTTTTCGGATTGGGATCATCAGCGTACATGGAACCATATATGGCATTTTCTGGAATACTAAGCAATCAATCTGAGGAAAATCCCGAGTTTTTCAACTGGAATAGAGTAGAGCTCCGCTATTGTGACGGTGCTTCATTTTCTGGGGACAGCGAACGTCAGGAAGCAAAACTGCAATTTAGAGGACAGCGTATATGGTTATCTGCAATGGACGATTTGATGTCAAAGGGAATGCGTGACGCGAAAAATGCTCTTCTTTGTGGTTGCTCTGCCGGCGGTCTGTCAGCAATTTTGCACTGTGATGAGTTTCGGGGCTTGTTTCCCACAAGTACAAAAGTGAAGTGCCTGAGTGATGCTGGATTTTTTCTTGACGCGGTTGATATTTCCGGTGAACGCACACTGAGGGATTATTATAGTGGTGTTGTAAGCTTGCAGGGGGTGCAAGAAAATCTGCCGCGATTCTGTACTAGTCACCTCGATCCTACTTCGTGCTTCTTCCCGGAGAACTTAATTGTGAACATTACAACCCCGCTATTTATACTCAATTCAGCATACGATTCGTGGCAGATCAATAACAGTTTAGTTCCACCATCAGCAGATCCGTGTGGCGAGTGGGAGAGTTGCAGATATAACCTTGCAAGTTGTTCTGCATCACAGATGCAAATTCTGCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAATTCTCAATGCCTAATCAAAATGGGATGTTCATAAATTCGTGTTTGGTGCACTGTCAAACGAAGGAAAGTGCATTGTTTGCTCATGAATTTCCAGCTCTTGGAAACAAGACAATTGGAGAAGCTCTTGGAGATTGGTATTTTGATAGATCAGAGGTTAAGGTTGTCGATGAAACTTGCTGCGATCTGGCTCTTGAATGA

Protein Analysis

411

Amino Acids

45.3

Weight (kDa)

4.85

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 50 - 401 2.1e-155 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccII CGCG 3 cut(s) 569, 701, 784
AciI CCGC 6 cut(s) 319, 445, 603, 701, 782, 857
AclWI GGATC 4 cut(s) 253, 352, 800, 920
AcsI RAATTY 3 cut(s) 990, 1069, 1121
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 3 cut(s) 356, 658, 793
AfiI CCNNNNNNNGG 1 cut(s) 1133
AgsI TTSAA 2 cut(s) 424, 1229
AhlI ACTAGT 1 cut(s) 794
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 8 cut(s) 123, 160, 179, 185, 442, 756, 1130, 1157
AluI AGCT 8 cut(s) 123, 160, 179, 185, 442, 756, 1130, 1157
Alw21I GWGCWC 2 cut(s) 444, 1086
Alw26I GTCTC 2 cut(s) 83, 104
Alw44I GTGCAC 1 cut(s) 1082
AlwI GGATC 4 cut(s) 253, 352, 800, 920
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 413
ApaLI GTGCAC 1 cut(s) 1082
ApeKI GCWGC 3 cut(s) 179, 182, 1212
ApoI RAATTY 3 cut(s) 990, 1069, 1121
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 4 cut(s) 16, 263, 728, 791
AvaI CYCGRG 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 1086
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 444
Bbv12I GWGCWC 2 cut(s) 444, 1086
BbvI GCAGC 3 cut(s) 166, 194, 1199
BccI CCATC 4 cut(s) 53, 106, 200, 925
BcgI CGANNNNNNTGC 2 cut(s) 867, 901
BciT130I CCWGG 1 cut(s) 128
BcnI CCSGG 1 cut(s) 827
BcoDI GTCTC 2 cut(s) 83, 104
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 4 cut(s) 180, 183, 782, 1213
BlsI GCNGC 4 cut(s) 181, 184, 783, 1214
Bme1390I CCNGG 2 cut(s) 128, 827
BmeT110I CYCGRG 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 96, 363
BmrFI CCNGG 2 cut(s) 128, 827
BmsI GCATC 3 cut(s) 670, 975, 986
BplI GAGNNNNNCTC 2 cut(s) 426, 458
BpuMI CCSGG 1 cut(s) 827
BsaBI GATNNNNATC 2 cut(s) 244, 343
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 713
Bsc4I CCNNNNNNNGG 1 cut(s) 1133
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 431
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 2 cut(s) 244, 343
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 211, 1065
BseJI GATNNNNATC 2 cut(s) 244, 343
BseLI CCNNNNNNNGG 1 cut(s) 1133
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 393, 665, 719
BseNI ACTGG 1 cut(s) 431
BseRI GAGGAG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 1086
BseXI GCAGC 3 cut(s) 166, 194, 1199
Bsh1236I CGCG 3 cut(s) 569, 701, 784
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 444, 1086
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 3 cut(s) 600, 714, 827
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 1 cut(s) 1133
BsmAI GTCTC 2 cut(s) 83, 104
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 488
BsmI GAATGC 2 cut(s) 564, 1027
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 2 cut(s) 444, 1086
Bsp143I GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
BspACI CCGC 6 cut(s) 319, 445, 603, 701, 782, 857
BspCNI CTCAG 3 cut(s) 394, 666, 720
BspFNI CGCG 3 cut(s) 569, 701, 784
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 2 cut(s) 96, 363
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 4 cut(s) 253, 352, 800, 920
BspQI GCTCTTC 1 cut(s) 585
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 6 cut(s) 94, 260, 458, 626, 905, 1088
Bst6I CTCTTC 2 cut(s) 66, 585
BstAPI GCANNNNNTGC 3 cut(s) 766, 994, 1112
BstC8I GCNNGC 2 cut(s) 601, 758
BstDEI CTNAG 4 cut(s) 389, 402, 674, 728
BstF5I GGATG 2 cut(s) 211, 1065
BstFNI CGCG 3 cut(s) 569, 701, 784
BstKTI GATC 7 cut(s) 248, 347, 808, 897, 928, 1184, 1219
BstMAI GTCTC 2 cut(s) 83, 104
BstMBI GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
BstMWI GCNNNNNNNGC 6 cut(s) 191, 575, 600, 766, 994, 1112
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 2 cut(s) 126, 825
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 3 cut(s) 569, 701, 784
BstV1I GCAGC 3 cut(s) 166, 194, 1199
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BtsCI GGATG 2 cut(s) 211, 1065
BtsIMutI CAGTG 3 cut(s) 622, 725, 1084
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 601, 758
CaiI CAGNNNCTG 1 cut(s) 218
CciI TCATGA 1 cut(s) 1117
Cfr10I RCCGGY 1 cut(s) 599
CseI GACGC 2 cut(s) 575, 707
Csp6I GTAC 3 cut(s) 355, 657, 792
CviAII CATG 5 cut(s) 151, 236, 288, 358, 1118
CviQI GTAC 3 cut(s) 355, 657, 792
DdeI CTNAG 4 cut(s) 389, 402, 674, 728
DpnI GATC 7 cut(s) 247, 346, 807, 896, 927, 1183, 1218
DpnII GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
Eam1104I CTCTTC 2 cut(s) 66, 585
EarI CTCTTC 2 cut(s) 66, 585
Ecl136II GAGCTC 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 5 cut(s) 154, 239, 291, 361, 1121
FaqI GGGAC 1 cut(s) 488
FatI CATG 5 cut(s) 150, 235, 287, 357, 1117
FauI CCCGC 1 cut(s) 864
Fnu4HI GCNGC 4 cut(s) 180, 183, 782, 1213
FokI GGATG 2 cut(s) 218, 1072
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 4 cut(s) 180, 183, 782, 1213
FspBI CTAG 1 cut(s) 795
GluI GCNGC 4 cut(s) 180, 183, 782, 1213
HapII CCGG 3 cut(s) 600, 714, 827
HgaI GACGC 2 cut(s) 575, 707
Hin1II CATG 5 cut(s) 154, 239, 291, 361, 1121
HindIII AAGCTT 1 cut(s) 754
HinfI GANTC 5 cut(s) 79, 786, 884, 1002, 1009
HpaII CCGG 3 cut(s) 600, 714, 827
HphI GGTGA 4 cut(s) 16, 263, 728, 791
Hpy166II GTNNAC 3 cut(s) 719, 844, 1084
Hpy188I TCNGA 5 cut(s) 47, 109, 338, 403, 1186
Hpy188III TCNNGA 9 cut(s) 83, 249, 381, 413, 488, 695, 1006, 1118, 1226
Hpy8I GTNNAC 3 cut(s) 719, 844, 1084
HpyAV CCTTC 2 cut(s) 284, 1090
HpyCH4III ACNGT 6 cut(s) 94, 260, 458, 626, 905, 1088
HpyCH4IV ACGT 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 575, 600, 766, 994, 1112
HpyF3I CTNAG 4 cut(s) 389, 402, 674, 728
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 5 cut(s) 154, 239, 291, 361, 1121
KroI GCCGGC 1 cut(s) 599
KroNI GCCGGC 1 cut(s) 601
Kzo9I GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 5 cut(s) 128, 191, 251, 284, 447
Lsp1109I GCAGC 3 cut(s) 166, 194, 1199
LweI GCATC 3 cut(s) 670, 975, 986
MaeI CTAG 1 cut(s) 795
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 5 cut(s) 28, 293, 452, 563, 797
MalI GATC 7 cut(s) 247, 346, 807, 896, 927, 1183, 1218
MboI GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
MboII GAAGA 4 cut(s) 83, 88, 572, 814
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 2 cut(s) 444, 1086
MluCI AATT 9 cut(s) 503, 615, 837, 871, 990, 1034, 1069, 1121, 1146
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 6 cut(s) 275, 397, 503, 723, 812, 1180
MroNI GCCGGC 1 cut(s) 599
MseI TTAA 3 cut(s) 135, 836, 1191
MslI CAYNNNNRTG 2 cut(s) 240, 627
MspI CCGG 3 cut(s) 600, 714, 827
MspR9I CCNGG 2 cut(s) 128, 827
MunI CAATTG 1 cut(s) 1146
Mva1269I GAATGC 2 cut(s) 564, 1027
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 3 cut(s) 569, 701, 784
MwoI GCNNNNNNNGC 6 cut(s) 191, 575, 600, 766, 994, 1112
NaeI GCCGGC 1 cut(s) 601
NciI CCSGG 1 cut(s) 827
NdeII GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
NgoMIV GCCGGC 1 cut(s) 599
NlaIII CATG 5 cut(s) 154, 239, 291, 361, 1121
NlaIV GGNNCC 2 cut(s) 96, 363
NmuCI GTSAC 3 cut(s) 452, 563, 797
PagI TCATGA 1 cut(s) 1117
PaqCI CACCTGC 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 585
PctI GAATGC 2 cut(s) 564, 1027
PdiI GCCGGC 1 cut(s) 601
PfeI GAWTC 5 cut(s) 79, 786, 884, 1002, 1009
PflMI CCANNNNNTGG 1 cut(s) 1133
PfoI TCCNGGA 1 cut(s) 825
PkrI GCNGC 4 cut(s) 181, 184, 783, 1214
Psp124BI GAGCTC 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 96, 363
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 1 cut(s) 925
RsaI GTAC 3 cut(s) 356, 658, 793
RsaNI GTAC 3 cut(s) 355, 657, 792
RseI CAYNNNNRTG 2 cut(s) 240, 627
SacI GAGCTC 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 3 cut(s) 135, 836, 1191
SatI GCNGC 4 cut(s) 180, 183, 782, 1213
Sau3AI GATC 7 cut(s) 245, 344, 805, 894, 925, 1181, 1216
ScrFI CCNGG 2 cut(s) 128, 827
SduI GDGCHC 2 cut(s) 444, 1086
SfaNI GCATC 3 cut(s) 670, 975, 986
SfcI CTRYAG 1 cut(s) 180
SmiMI CAYNNNNRTG 2 cut(s) 240, 627
SpeI ACTAGT 1 cut(s) 794
Sse9I AATT 9 cut(s) 503, 615, 837, 871, 990, 1034, 1069, 1121, 1146
SsiI CCGC 6 cut(s) 319, 445, 603, 701, 782, 857
SspMI CTAG 1 cut(s) 795
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 126, 825
TaaI ACNGT 6 cut(s) 94, 260, 458, 626, 905, 1088
TaiI ACGT 1 cut(s) 487
TaqI TCGA 2 cut(s) 804, 1200
TasI AATT 9 cut(s) 503, 615, 837, 871, 990, 1034, 1069, 1121, 1146
TatI WGTACW 2 cut(s) 656, 791
TauI GCSGC 1 cut(s) 784
TfiI GAWTC 5 cut(s) 79, 786, 884, 1002, 1009
Tru1I TTAA 3 cut(s) 135, 836, 1191
Tru9I TTAA 3 cut(s) 135, 836, 1191
TscAI CASTG 3 cut(s) 629, 732, 1091
TseFI GTSAC 3 cut(s) 452, 563, 797
TseI GCWGC 3 cut(s) 179, 182, 1212
Tsp45I GTSAC 3 cut(s) 452, 563, 797
TspDTI ATGAA 4 cut(s) 453, 1054, 1134, 1218
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 3 cut(s) 629, 732, 1091
Van91I CCANNNNNTGG 1 cut(s) 1133
VneI GTGCAC 1 cut(s) 1082
XapI RAATTY 3 cut(s) 990, 1069, 1121
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.