Rh3DG093300

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001553
Physical Location & Seq
Reverse (-)
7268054 .. 7271967
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG093300.1

Sequence Viewer

Length: 912 bp
ATGGAAAAACAGTTGCTATTTACCGGAATACTAAGCAATAAAGCTGAAGAAAATCCAGATTTTTTCAACTGGAATAGAGTAAAGCTTCGCTATTGTGATGGTGCTTCTTTTTCTGGGGATAGTGAAAATAAGGCAGCACAACTACAATTCAGAGGACAACGTATATGGTTGTCTGCAATGGAAGATTTGATGTCAAAGGGCATGCGCTATGCTAAGCAGGCTCTTCTTTCAGGTTGCTCTGCTGGTGGCCTGGCATCTATTTTGCACTGCGATGAGTTCCAGGGCTTATTTTCAAGAAGTACAAAAGTGAAGTGCCTAAGTGATGCTGGATTGTTTCTTGATGCGGCTGATGTTTCTGGTGGACGCACACTCAGGAATTTATATGGTGGTGTTGTTGGCTTGCAGGGGGTGCAACAAACTCTACCACGCATTTGTCTTAACCATCTTGATCCTACATCGTGCTTCTTCCCTCAGAACTTAATCAACAGCATTAAAACCCCACTGTTTATACTCAATGCAGCATATGATTCATGGCAGATCCAATCCAGTTTAGCTCCACCATCAGCAGATCCTCACGGTTACTGGAAGAACTGCAGATTAAATCATGCAAAGTGTTCCCCAGAACAGATTCAATATCTGCAAGGATTCAGGAATCAAATGCTGAATGTAGTGAAACAATTTGCGCAGTCTGATAAAAATGGGTTGTTTATAAATTCATGCTTTGCTCATTGCCAAAGTGAGAGGCAGGATACATGGTTTGCTGACAATTCTCCAGTCATCAGAAACAAGGCAATTGCACTAGCTGTTGGGGATTGGTATTTTGAACGAACCGGTGTTAAGGGTGCTAAGGAAGTTGATTGTCCATACCCTTGTGACAACTCTTGCCACAATCTGGTCTTCCAACAAGCATAG

Protein Analysis

303

Amino Acids

33.83

Weight (kDa)

8.19

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 296 2.2e-133 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
Acc16I TGCGCA 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 892
AciI CCGC 1 cut(s) 344
AclWI GGATC 3 cut(s) 443, 532, 563
AcsI RAATTY 2 cut(s) 376, 712
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 1 cut(s) 301
AfiI CCNNNNNNNGG 1 cut(s) 892
AgeI ACCGGT 1 cut(s) 830
AgsI TTSAA 4 cut(s) 67, 294, 632, 824
AjnI CCWGG 2 cut(s) 249, 279
AluBI AGCT 4 cut(s) 44, 85, 554, 803
AluI AGCT 4 cut(s) 44, 85, 554, 803
AlwI GGATC 3 cut(s) 443, 532, 563
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 134, 518
ApoI RAATTY 2 cut(s) 376, 712
AsiGI ACCGGT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 627
AspLEI GCGC 2 cut(s) 207, 685
BbsI GAAGAC 1 cut(s) 889
BbvI GCAGC 2 cut(s) 146, 530
BccI CCATC 3 cut(s) 92, 450, 568
BciT130I CCWGG 2 cut(s) 251, 281
BciVI GTATCC 1 cut(s) 742
BfaI CTAG 1 cut(s) 800
BfmI CTRYAG 1 cut(s) 592
BfuI GTATCC 1 cut(s) 742
BisI GCNGC 3 cut(s) 135, 345, 519
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 3 cut(s) 136, 346, 520
Bme1390I CCNGG 2 cut(s) 251, 281
BmrFI CCNGG 2 cut(s) 251, 281
BmsI GCATC 3 cut(s) 263, 313, 331
BpiI GAAGAC 1 cut(s) 889
BpmI CTGGAG 1 cut(s) 756
Bpu10I CCTNAGC 1 cut(s) 846
Bpu1102I GCTNAGC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 2 cut(s) 23, 830
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 4 cut(s) 74, 546, 587, 773
Bse3DI GCAATG 2 cut(s) 183, 727
BseBI CCWGG 2 cut(s) 251, 281
BseDI CCNNGG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 2 cut(s) 183, 727
BseMII CTCAG 2 cut(s) 385, 485
BseNI ACTGG 4 cut(s) 74, 546, 587, 773
BseXI GCAGC 2 cut(s) 146, 530
BshFI GGCC 1 cut(s) 249
BshTI ACCGGT 1 cut(s) 830
BsiSI CCGG 2 cut(s) 24, 831
BslI CCNNNNNNNGG 1 cut(s) 892
BsnI GGCC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 448, 537, 568
Bsp1720I GCTNAGC 1 cut(s) 213
BspACI CCGC 1 cut(s) 344
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 2 cut(s) 384, 484
BspMAI CTGCAG 1 cut(s) 596
BspPI GGATC 3 cut(s) 443, 532, 563
BspQI GCTCTTC 1 cut(s) 228
BsrDI GCAATG 2 cut(s) 183, 727
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 4 cut(s) 74, 546, 587, 773
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 3 cut(s) 448, 537, 568
Bst2UI CCWGG 2 cut(s) 251, 281
Bst4CI ACNGT 3 cut(s) 12, 504, 578
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 409
BstC8I GCNNGC 3 cut(s) 203, 219, 401
BstDEI CTNAG 6 cut(s) 32, 213, 317, 371, 471, 846
BstHHI GCGC 2 cut(s) 207, 685
BstKTI GATC 3 cut(s) 451, 540, 571
BstMBI GATC 3 cut(s) 448, 537, 568
BstMWI GCNNNNNNNGC 2 cut(s) 218, 409
BstNI CCWGG 2 cut(s) 251, 281
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 2 cut(s) 249, 279
BstSFI CTRYAG 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 146, 530
BstV2I GAAGAC 1 cut(s) 889
BstX2I RGATCY 2 cut(s) 537, 568
BstYI RGATCY 2 cut(s) 537, 568
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 2 cut(s) 265, 500
Cac8I GCNNGC 3 cut(s) 203, 219, 401
CfoI GCGC 2 cut(s) 207, 685
Cfr10I RCCGGY 1 cut(s) 830
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 300
CspAI ACCGGT 1 cut(s) 830
CviAII CATG 5 cut(s) 202, 531, 605, 717, 753
CviJI RGCY 9 cut(s) 44, 85, 221, 249, 285, 347, 399, 554, 803
CviKI_1 RGCY 9 cut(s) 44, 85, 221, 249, 285, 347, 399, 554, 803
CviQI GTAC 1 cut(s) 300
DdeI CTNAG 6 cut(s) 32, 213, 317, 371, 471, 846
DpnI GATC 3 cut(s) 450, 539, 570
DpnII GATC 3 cut(s) 448, 537, 568
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 249, 279
FaeI CATG 5 cut(s) 205, 534, 608, 720, 756
FatI CATG 5 cut(s) 201, 530, 604, 716, 752
FauNDI CATATG 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 135, 345, 519
Fsp4HI GCNGC 3 cut(s) 135, 345, 519
FspBI CTAG 1 cut(s) 800
FspI TGCGCA 1 cut(s) 684
GlaI GCGC 2 cut(s) 206, 684
GluI GCNGC 3 cut(s) 135, 345, 519
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 1 cut(s) 249
HapII CCGG 2 cut(s) 24, 831
HgaI GACGC 1 cut(s) 372
HhaI GCGC 2 cut(s) 207, 685
Hin1II CATG 5 cut(s) 205, 534, 608, 720, 756
Hin6I GCGC 2 cut(s) 205, 683
HinP1I GCGC 2 cut(s) 205, 683
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 4 cut(s) 527, 628, 645, 652
HpaII CCGG 2 cut(s) 24, 831
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 4 cut(s) 152, 474, 691, 782
Hpy188III TCNNGA 6 cut(s) 56, 294, 338, 373, 446, 649
Hpy8I GTNNAC 1 cut(s) 362
HpyCH4III ACNGT 3 cut(s) 12, 504, 578
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 9 cut(s) 176, 265, 403, 412, 518, 594, 608, 640, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 409
HpyF3I CTNAG 6 cut(s) 32, 213, 317, 371, 471, 846
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 5 cut(s) 205, 534, 608, 720, 756
HspAI GCGC 2 cut(s) 205, 683
Kzo9I GATC 3 cut(s) 448, 537, 568
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 1 cut(s) 559
Lsp1109I GCAGC 2 cut(s) 146, 530
LweI GCATC 3 cut(s) 263, 313, 331
MaeI CTAG 1 cut(s) 800
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 578, 872
MalI GATC 3 cut(s) 450, 539, 570
MboI GATC 3 cut(s) 448, 537, 568
MboII GAAGA 6 cut(s) 59, 194, 215, 457, 598, 889
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 2 cut(s) 537, 568
MluCI AATT 6 cut(s) 146, 376, 677, 712, 766, 792
MnlI CCTC 4 cut(s) 146, 480, 582, 735
MroXI GAANNNNTTC 1 cut(s) 627
MseI TTAA 5 cut(s) 438, 479, 492, 599, 837
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 2 cut(s) 24, 831
MspR9I CCNGG 2 cut(s) 251, 281
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 2 cut(s) 251, 281
MwoI GCNNNNNNNGC 2 cut(s) 218, 409
NdeI CATATG 1 cut(s) 523
NdeII GATC 3 cut(s) 448, 537, 568
NlaIII CATG 5 cut(s) 205, 534, 608, 720, 756
NmuCI GTSAC 1 cut(s) 872
NsbI TGCGCA 1 cut(s) 684
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 627
PfeI GAWTC 4 cut(s) 527, 628, 645, 652
PflMI CCANNNNNTGG 1 cut(s) 892
PinAI ACCGGT 1 cut(s) 830
PkrI GCNGC 3 cut(s) 136, 346, 520
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 249, 279
PspGI CCWGG 2 cut(s) 249, 279
PstI CTGCAG 1 cut(s) 596
PsuI RGATCY 2 cut(s) 537, 568
RsaI GTAC 1 cut(s) 301
RsaNI GTAC 1 cut(s) 300
RseI CAYNNNNRTG 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 5 cut(s) 438, 479, 492, 599, 837
SatI GCNGC 3 cut(s) 135, 345, 519
Sau3AI GATC 3 cut(s) 448, 537, 568
ScrFI CCNGG 2 cut(s) 251, 281
SetI ASST 6 cut(s) 46, 87, 163, 235, 556, 805
SfaNI GCATC 3 cut(s) 263, 313, 331
SfcI CTRYAG 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 270
SphI GCATGC 1 cut(s) 205
Sse9I AATT 6 cut(s) 146, 376, 677, 712, 766, 792
SsiI CCGC 1 cut(s) 344
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 2 cut(s) 249, 279
TaaI ACNGT 3 cut(s) 12, 504, 578
TaiI ACGT 1 cut(s) 163
TasI AATT 6 cut(s) 146, 376, 677, 712, 766, 792
TatI WGTACW 1 cut(s) 299
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 4 cut(s) 527, 628, 645, 652
Tru1I TTAA 5 cut(s) 438, 479, 492, 599, 837
Tru9I TTAA 5 cut(s) 438, 479, 492, 599, 837
TscAI CASTG 2 cut(s) 272, 507
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 2 cut(s) 134, 518
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 2 cut(s) 519, 705
TspRI CASTG 2 cut(s) 272, 507
Van91I CCANNNNNTGG 1 cut(s) 892
XapI RAATTY 2 cut(s) 376, 712
XceI RCATGY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 627
XspI CTAG 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.