FvH4_6g08640

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001553
Physical Location & Seq
Reverse (-)
5149734 .. 5153545
3812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08640.t3

Sequence Viewer

Length: 912 bp
ATGGAAAAACAGGTGCAATTTACCGGAATACTCAGCAATAAAGCTGAAGAAAATCCAGATTTTTTTAACTGGAATAGAGTCAAGCTTCGCTATTGTGATGGTGCTTCTTTTTCTGGGGATAGTGAAAATAAGGCAGCACAACTACAATTCAGAGGACAACGTATATGGTTGTCTGCTATGGAAGATTTGATGTCAAAGGGCATGCGCTATGCTAATCAGGCTCTTCTTTCTGGTTGTTCTGCTGGTGGCCTGGCATCTATTTTGCACTGTGATGAGTTCCGGGGCTTATTTTCAACAAGAACAAAAGTGAAGTGCCTAAGTGATGCTGGATTGTTTCTTGATGCGGCTGATGTTTCTGGTGGACGCACGCTCAGGAATTTATATGGTGGCGTTGTTGGCTTGCAGGGGGTGCAAGAAACTCTACCACGAATTTGTCTTAACCATCTTGATCCTACATCGTGCTTCTTCCCTCAGAATTTAATCAACAGTATTAAAACCCCACTGTTTATACTCAATGCAGCATATGATTCCTGGCAGATCCAATCCAGTTTAGCTCCACCCTCAGCAGATCCTCGTGGTTACTGGAAGGACTGCAGATTAAATCATGCAAAATGTTCCCCAAAACAGATTCAATTTCTGCAAGGATTCAGGAATCAAATGCTGAATGTAGTAAGACAATTCGCAGTGTCTGATAAAAACGGGTTGTTTATAAATTCATGCTTTGCCCATTGCCAAAGTGAGAGGCAGGATACATGGTTTGCTGACAATTCTCCAGTCATCAAAAACAAGGCAATTGCACTAGCTGTCGGGGACTGGTATTTTGAACGAACCGGTGTTAAGGGTACCAAGGCATTTGATTGTCCATACCCTTGTGACAACTCTTGCCACAATCTGGTCTTCCAACGAGCATAG

Protein Analysis

304

Amino Acids

33.89

Weight (kDa)

8.65

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 1 - 296 2.3e-132 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
Acc65I GGTACC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 892
AciI CCGC 1 cut(s) 344
AclWI GGATC 3 cut(s) 443, 532, 563
AcsI RAATTY 4 cut(s) 376, 429, 475, 712
AcuI CTGAAG 1 cut(s) 66
AfaI GTAC 1 cut(s) 844
AfiI CCNNNNNNNGG 1 cut(s) 892
AgeI ACCGGT 1 cut(s) 830
AgsI TTSAA 3 cut(s) 294, 632, 824
AjnI CCWGG 2 cut(s) 249, 530
AluBI AGCT 4 cut(s) 44, 85, 554, 803
AluI AGCT 4 cut(s) 44, 85, 554, 803
AlwI GGATC 3 cut(s) 443, 532, 563
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 2 cut(s) 134, 518
ApoI RAATTY 4 cut(s) 376, 429, 475, 712
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
AsiGI ACCGGT 1 cut(s) 830
Asp718I GGTACC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 207
AsuC2I CCSGG 1 cut(s) 281
BanI GGYRCC 1 cut(s) 842
BauI CACGAG 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 889
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 2 cut(s) 146, 530
BccI CCATC 2 cut(s) 92, 450
BciT130I CCWGG 2 cut(s) 251, 532
BciVI GTATCC 1 cut(s) 742
BcnI CCSGG 1 cut(s) 281
BfaI CTAG 1 cut(s) 800
BfmI CTRYAG 1 cut(s) 592
BfuI GTATCC 1 cut(s) 742
BisI GCNGC 3 cut(s) 135, 345, 519
BlsI GCNGC 3 cut(s) 136, 346, 520
Bme1390I CCNGG 3 cut(s) 251, 281, 532
BmiI GGNNCC 1 cut(s) 844
BmrFI CCNGG 3 cut(s) 251, 281, 532
BmsI GCATC 3 cut(s) 263, 313, 331
BpiI GAAGAC 1 cut(s) 889
BpmI CTGGAG 1 cut(s) 756
Bpu10I CCTNAGC 2 cut(s) 371, 562
BpuMI CCSGG 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 280, 846
BsaWI WCCGGW 2 cut(s) 23, 830
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 5 cut(s) 74, 546, 587, 773, 818
Bse3DI GCAATG 1 cut(s) 727
BseBI CCWGG 2 cut(s) 251, 532
BseDI CCNNGG 2 cut(s) 280, 846
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 4 cut(s) 46, 385, 485, 576
BseNI ACTGG 5 cut(s) 74, 546, 587, 773, 818
BseXI GCAGC 2 cut(s) 146, 530
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 842
BshTI ACCGGT 1 cut(s) 830
BsiSI CCGG 3 cut(s) 24, 280, 831
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 1 cut(s) 892
BsmFI GGGAC 1 cut(s) 824
BsnI GGCC 1 cut(s) 249
Bsp143I GATC 3 cut(s) 448, 537, 568
BspACI CCGC 1 cut(s) 344
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 4 cut(s) 45, 384, 484, 575
BspLI GGNNCC 1 cut(s) 844
BspMAI CTGCAG 1 cut(s) 596
BspPI GGATC 3 cut(s) 443, 532, 563
BspQI GCTCTTC 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 5 cut(s) 74, 546, 587, 773, 818
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 2 cut(s) 280, 846
BssMI GATC 3 cut(s) 448, 537, 568
BssSI CACGAG 1 cut(s) 573
BssT1I CCWWGG 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 2 cut(s) 251, 532
Bst4CI ACNGT 3 cut(s) 269, 488, 504
Bst6I CTCTTC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 409
BstC8I GCNNGC 3 cut(s) 203, 368, 401
BstDEI CTNAG 5 cut(s) 32, 317, 371, 471, 562
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 3 cut(s) 451, 540, 571
BstMBI GATC 3 cut(s) 448, 537, 568
BstMWI GCNNNNNNNGC 3 cut(s) 218, 396, 409
BstNI CCWGG 2 cut(s) 251, 532
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 3 cut(s) 249, 279, 530
BstSFI CTRYAG 1 cut(s) 592
BstV1I GCAGC 2 cut(s) 146, 530
BstV2I GAAGAC 1 cut(s) 889
BstX2I RGATCY 2 cut(s) 537, 568
BstYI RGATCY 2 cut(s) 537, 568
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 3 cut(s) 265, 500, 690
Cac8I GCNNGC 3 cut(s) 203, 368, 401
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 830
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 843
CspAI ACCGGT 1 cut(s) 830
CviAII CATG 4 cut(s) 202, 605, 717, 753
CviJI RGCY 9 cut(s) 44, 85, 221, 249, 285, 347, 399, 554, 803
CviKI_1 RGCY 9 cut(s) 44, 85, 221, 249, 285, 347, 399, 554, 803
CviQI GTAC 1 cut(s) 843
DdeI CTNAG 5 cut(s) 32, 317, 371, 471, 562
DpnI GATC 3 cut(s) 450, 539, 570
DpnII GATC 3 cut(s) 448, 537, 568
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 846
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 249, 530
EcoT14I CCWWGG 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 4 cut(s) 205, 608, 720, 756
FaqI GGGAC 1 cut(s) 824
FatI CATG 4 cut(s) 201, 604, 716, 752
FauNDI CATATG 1 cut(s) 523
Fnu4HI GCNGC 3 cut(s) 135, 345, 519
Fsp4HI GCNGC 3 cut(s) 135, 345, 519
FspBI CTAG 1 cut(s) 800
GlaI GCGC 1 cut(s) 206
GluI GCNGC 3 cut(s) 135, 345, 519
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 1 cut(s) 249
HapII CCGG 3 cut(s) 24, 280, 831
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 207
Hin1II CATG 4 cut(s) 205, 608, 720, 756
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 5 cut(s) 78, 527, 628, 645, 652
HpaII CCGG 3 cut(s) 24, 280, 831
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 3 cut(s) 152, 474, 691
Hpy188III TCNNGA 5 cut(s) 56, 338, 373, 446, 649
Hpy8I GTNNAC 1 cut(s) 362
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 3 cut(s) 269, 488, 504
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 9 cut(s) 16, 265, 403, 412, 518, 594, 608, 640, 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 396, 409
HpyF3I CTNAG 5 cut(s) 32, 317, 371, 471, 562
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 4 cut(s) 205, 608, 720, 756
HspAI GCGC 1 cut(s) 205
KpnI GGTACC 1 cut(s) 846
Kzo9I GATC 3 cut(s) 448, 537, 568
LguI GCTCTTC 1 cut(s) 228
LmnI GCTCC 1 cut(s) 559
Lsp1109I GCAGC 2 cut(s) 146, 530
LweI GCATC 3 cut(s) 263, 313, 331
MaeI CTAG 1 cut(s) 800
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 2 cut(s) 578, 872
MalI GATC 3 cut(s) 450, 539, 570
MboI GATC 3 cut(s) 448, 537, 568
MboII GAAGA 5 cut(s) 59, 194, 215, 457, 889
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 2 cut(s) 537, 568
MlyI GAGTC 1 cut(s) 87
MnlI CCTC 5 cut(s) 146, 480, 571, 582, 735
MseI TTAA 6 cut(s) 66, 438, 479, 492, 599, 837
MslI CAYNNNNRTG 1 cut(s) 270
MspI CCGG 3 cut(s) 24, 280, 831
MspR9I CCNGG 3 cut(s) 251, 281, 532
MunI CAATTG 1 cut(s) 792
MvaI CCWGG 2 cut(s) 251, 532
MwoI GCNNNNNNNGC 3 cut(s) 218, 396, 409
NciI CCSGG 1 cut(s) 281
NdeI CATATG 1 cut(s) 523
NdeII GATC 3 cut(s) 448, 537, 568
NlaIII CATG 4 cut(s) 205, 608, 720, 756
NlaIV GGNNCC 1 cut(s) 844
NmuCI GTSAC 1 cut(s) 872
NspI RCATGY 1 cut(s) 205
PaeI GCATGC 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 228
PfeI GAWTC 4 cut(s) 527, 628, 645, 652
PflMI CCANNNNNTGG 1 cut(s) 892
PinAI ACCGGT 1 cut(s) 830
PkrI GCNGC 3 cut(s) 136, 346, 520
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PsiI TTATAA 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 249, 530
PspGI CCWGG 2 cut(s) 249, 530
PspN4I GGNNCC 1 cut(s) 844
PstI CTGCAG 1 cut(s) 596
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 537, 568
RsaI GTAC 1 cut(s) 844
RsaNI GTAC 1 cut(s) 843
RseI CAYNNNNRTG 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 6 cut(s) 66, 438, 479, 492, 599, 837
SatI GCNGC 3 cut(s) 135, 345, 519
Sau3AI GATC 3 cut(s) 448, 537, 568
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 3 cut(s) 251, 281, 532
SetI ASST 6 cut(s) 15, 46, 87, 163, 556, 805
SfaNI GCATC 3 cut(s) 263, 313, 331
SfcI CTRYAG 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 270
SphI GCATGC 1 cut(s) 205
SsiI CCGC 1 cut(s) 344
SspMI CTAG 1 cut(s) 800
StyD4I CCNGG 3 cut(s) 249, 279, 530
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 3 cut(s) 269, 488, 504
TaiI ACGT 1 cut(s) 163
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 4 cut(s) 527, 628, 645, 652
Tru1I TTAA 6 cut(s) 66, 438, 479, 492, 599, 837
Tru9I TTAA 6 cut(s) 66, 438, 479, 492, 599, 837
TscAI CASTG 3 cut(s) 272, 507, 690
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 2 cut(s) 134, 518
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 1 cut(s) 705
TspRI CASTG 3 cut(s) 272, 507, 690
Van91I CCANNNNNTGG 1 cut(s) 892
XapI RAATTY 4 cut(s) 376, 429, 475, 712
XceI RCATGY 1 cut(s) 205
XspI CTAG 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.