Rh3CG092500

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001553
Physical Location & Seq
Reverse (-)
7234430 .. 7238904
4475 bp
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UTR
Exon/CDS
Intron
Rh3CG092500.1

Sequence Viewer

Length: 576 bp
ATGGTGAGGTTCTTGTGGGTTTGCATTGTAGTAGCATTTTGTTTCAGCAAATGGGTTGATGGGTTTGTGCAGTACAGTCATAGAGGAGATCCCAAAACTGAAATTATAAATAACACACTGATGGTATCGATAACCCTCATTCGAGGAGCTGGGGCCAAAGGAGCAGTCTGCTTGGATGGAACATTGCCTGCTTATTATTTTAACCGTGGACATGGGTCAGGGGCAAATAGTTGGGTCGTTCACTTTCAGGGTGGAGGATGGTGTAGCAACATCACAGAATGTGTTGCTCGCACAAAAACTCAGTTTGGATCATCAAGATACATGTTACCAGAGACGGGGTTCACTGGAATATTAAGCTTCAAAGCTGAAGAAAATCCAGATTTCTTCAACTGGAATAGAGTAATGCTTCCCTCTTGTGACGGTGCTTCTTTTTCTGGGGACAGTGAAAATAAGGTTCGCAGGATGTTCTTGCCATTTACATTTACCTTATACTTCTCCATCTGTTTCCCATATATCTACAGATTTGCACCAATTCAGTTAAACCATACGAACTTAGGAAGAGTATGGGAGATGTAG

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

8.71

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 38 - 155 3.5e-50 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 107
AclWI GGATC 2 cut(s) 83, 316
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 335
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 2 cut(s) 361, 388
AluBI AGCT 3 cut(s) 149, 357, 365
AluI AGCT 3 cut(s) 149, 357, 365
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 2 cut(s) 83, 316
AoxI GGCC 1 cut(s) 153
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
AspS9I GGNCC 1 cut(s) 153
AsuHPI GGTGA 1 cut(s) 16
BccI CCATC 5 cut(s) 53, 115, 170, 252, 506
BcoDI GTCTC 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 153
BmiI GGNNCC 1 cut(s) 154
BoxI GACNNNNGTC 1 cut(s) 214
Bsa29I ATCGAT 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 349, 395
Bse3DI GCAATG 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 3 cut(s) 181, 263, 468
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 1 cut(s) 182
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 349, 395
BseRI GAGGAG 2 cut(s) 99, 159
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 89
BshFI GGCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 128
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 326
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 88, 308
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 313
BspDI ATCGAT 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 154
BspPI GGATC 2 cut(s) 83, 316
BsrDI GCAATG 1 cut(s) 182
BsrI ACTGG 2 cut(s) 349, 395
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 2 cut(s) 88, 308
Bst4CI ACNGT 4 cut(s) 77, 206, 422, 443
Bst6I CTCTTC 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 189, 289
BstDEI CTNAG 2 cut(s) 300, 553
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 3 cut(s) 181, 263, 468
BstKTI GATC 2 cut(s) 91, 311
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 2 cut(s) 88, 308
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNSI RCATGY 1 cut(s) 325
BstPAI GACNNNNGTC 1 cut(s) 214
BstSFI CTRYAG 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 128
BsuRI GGCC 1 cut(s) 155
BsuTUI ATCGAT 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 3 cut(s) 181, 263, 468
BtsIMutI CAGTG 3 cut(s) 116, 342, 448
Cac8I GCNNGC 2 cut(s) 189, 289
Cfr13I GGNCC 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 128
Csp6I GTAC 1 cut(s) 73
CviAII CATG 2 cut(s) 212, 322
CviJI RGCY 4 cut(s) 149, 155, 357, 365
CviKI_1 RGCY 4 cut(s) 149, 155, 357, 365
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 2 cut(s) 300, 553
DpnI GATC 2 cut(s) 90, 310
DpnII GATC 2 cut(s) 88, 308
Eam1104I CTCTTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 387
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 215, 325
FaiI YATR 9 cut(s) 81, 107, 213, 323, 490, 511, 513, 546, 565
FaqI GGGAC 1 cut(s) 452
FatI CATG 2 cut(s) 211, 321
FokI GGATG 3 cut(s) 188, 270, 475
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 1 cut(s) 155
Hin1II CATG 2 cut(s) 215, 325
HindIII AAGCTT 1 cut(s) 355
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 209, 241, 342
Hpy188III TCNNGA 2 cut(s) 315, 377
Hpy8I GTNNAC 3 cut(s) 209, 241, 342
HpyCH4III ACNGT 4 cut(s) 77, 206, 422, 443
HpyCH4V TGCA 3 cut(s) 24, 70, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 2 cut(s) 300, 553
Hsp92II CATG 2 cut(s) 215, 325
Kzo9I GATC 2 cut(s) 88, 308
LmnI GCTCC 2 cut(s) 146, 161
MaeIII GTNAC 2 cut(s) 324, 416
MalI GATC 2 cut(s) 90, 310
MboI GATC 2 cut(s) 88, 308
MboII GAAGA 3 cut(s) 376, 380, 570
MflI RGATCY 1 cut(s) 88
MluCI AATT 2 cut(s) 102, 531
MnlI CCTC 5 cut(s) 77, 137, 146, 248, 421
MseI TTAA 3 cut(s) 201, 353, 539
MslI CAYNNNNRTG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 2 cut(s) 88, 308
NlaIII CATG 2 cut(s) 215, 325
NlaIV GGNNCC 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 325
PciI ACATGT 1 cut(s) 321
PscI ACATGT 1 cut(s) 321
PshAI GACNNNNGTC 1 cut(s) 214
PsiI TTATAA 1 cut(s) 107
PspFI CCCAGC 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 1 cut(s) 153
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 3 cut(s) 201, 353, 539
Sau3AI GATC 2 cut(s) 88, 308
Sau96I GGNCC 1 cut(s) 153
SetI ASST 6 cut(s) 11, 151, 359, 367, 456, 488
SfcI CTRYAG 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 119
Sse9I AATT 2 cut(s) 102, 531
SspI AATATT 1 cut(s) 351
TaaI ACNGT 4 cut(s) 77, 206, 422, 443
TaqI TCGA 2 cut(s) 128, 142
TasI AATT 2 cut(s) 102, 531
TatI WGTACW 1 cut(s) 72
Tru1I TTAA 3 cut(s) 201, 353, 539
Tru9I TTAA 3 cut(s) 201, 353, 539
TscAI CASTG 3 cut(s) 123, 349, 448
TseFI GTSAC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 416
TspRI CASTG 3 cut(s) 123, 349, 448
XceI RCATGY 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.