RLG00000025136

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001553
Physical Location & Seq
Forward (+)
42315642 .. 42318979
3338 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025136

Sequence Viewer

Length: 1254 bp
ATGAAGTTTTTGTGGGTTGGCATTGTTGTAGTGCTAGTTTTCACCAAATGGGCTAATGGGTTTGAGGAGCATAGTTTTAATGAAACAGAGCTGTCCTGGTTGGAGGGCTATGGAGAATCCAAGGCTGCTGTAACTGGGTTGATGGTAGGACTAACCCTTATTCCAGGAGCTGGTGCCAAAGGAGCAGTCTGCCTGGATGGAACGTTACCCGGGTATCATTTGCATCGTGGGTACGGGTCAGGAGCAAATAGTTGGCTTATTCAATTGGAGGGGGGAGGATGGTGTAACACCATTAGAAATTGTGTTTACCGCAAAACAACAAGGCGGGGATCATCAAAATTTATGGAAAAACAGTTGCTATTTACAGGAATACTAAGCAATAAAGCTGAAGAAAATCCAGATTTTTTCAACTGGAATAGAGTAAAGCTTCGCTATTGTGATGGTGCTTCTTTTTCTGGGGATAGTGAAAATAAGGCAGCACAGCTACATTTCAAAGGACAACGTATATGGTTGTCTGCAATGGAAGATTTGATGTCAAAGGGCATGCGCTATGCTAAGCAGGCTCTTCTTTCAGGTTGCTCTGCTGGCGGCCTGGCATCTATTTTGCACTGTGATGAGTTCCGGGGCTTATTTTCAAGAAGTACAAAAGTGAAGTGCCTAAGTGATGCTGGATTGTTTCTTGACGCGGCTGATGTTTCTGGTGGACGCACACTCAGGAATTTGTATGGTGGTGTTGTTGGCTTGCAGGGGGTGCAACAAACTCTACCACGCATTTGTCTTAACCATCTTGATCCTACATCGTGCTTCTTCCCTCAGAACTTAATCAACAGCATTAAAACCCCACTGTTTATACTCAATGCAGCATATGATTCATGGCAGATCCAATCCAGTTTAGCTCCACCATCAGCAGATCCTCACGGTTACTGGAAGAACTGCAGATTAAATCATGCAAACTGTTCCCCAAAACAGATTCAATATCTGCAAGGATTCAGAAATCAAATGCTGAATGTAGTGAAACAATTCGCGCTGTCTGATAAAAATGGGTTGTTTATAAATTCATGCTTTGCTCATTGCCAAAGTGAGAGGCAGGATACATGGTTTGCTGACAATTCTCCAGTCATCAGAAACAAGGCAATTGCACTAGCTGTTGGGGATTGGTATTTTGAACGAACCGGTGTTAAGGGTGCTAAGGCAGTTGATTGTCCATACCCTTGTGACAACTCTTGCCACAATCTGGTCTTCCAACGAGCATAG

Protein Analysis

418

Amino Acids

46.19

Weight (kDa)

8.85

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 45 - 410 1.7e-170 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
AccB1I GGYRCC 1 cut(s) 173
AccB7I CCANNNNNTGG 2 cut(s) 170, 1234
AccII CGCG 2 cut(s) 686, 1025
AciI CCGC 4 cut(s) 310, 325, 588, 686
AclI AACGTT 1 cut(s) 203
AclWI GGATC 4 cut(s) 337, 785, 874, 905
AcsI RAATTY 3 cut(s) 338, 718, 1054
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 2 cut(s) 233, 643
AfiI CCNNNNNNNGG 2 cut(s) 170, 1234
AgeI ACCGGT 1 cut(s) 1172
AgsI TTSAA 6 cut(s) 263, 409, 493, 636, 974, 1166
AjnI CCWGG 4 cut(s) 95, 163, 192, 591
AluBI AGCT 7 cut(s) 91, 170, 386, 427, 484, 896, 1145
AluI AGCT 7 cut(s) 91, 170, 386, 427, 484, 896, 1145
AlwI GGATC 4 cut(s) 337, 785, 874, 905
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 589
ApeKI GCWGC 3 cut(s) 125, 476, 860
ApoI RAATTY 3 cut(s) 338, 718, 1054
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AsiGI ACCGGT 1 cut(s) 1172
Asp700I GAANNNNTTC 1 cut(s) 1019
AspLEI GCGC 2 cut(s) 549, 1027
AsuC2I CCSGG 3 cut(s) 210, 211, 623
AsuHPI GGTGA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 209
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BanI GGYRCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 1231
BbvI GCAGC 3 cut(s) 112, 488, 872
BccI CCATC 6 cut(s) 136, 191, 273, 434, 792, 910
BciT130I CCWGG 4 cut(s) 97, 165, 194, 593
BciVI GTATCC 1 cut(s) 1084
BcnI CCSGG 3 cut(s) 210, 211, 623
BfaI CTAG 2 cut(s) 35, 1142
BfmI CTRYAG 1 cut(s) 934
BfuI GTATCC 1 cut(s) 1084
BisI GCNGC 5 cut(s) 126, 477, 589, 687, 861
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 5 cut(s) 127, 478, 590, 688, 862
Bme1390I CCNGG 7 cut(s) 97, 165, 194, 210, 211, 593, 623
BmeT110I CYCGRG 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 7 cut(s) 97, 165, 194, 210, 211, 593, 623
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 3 cut(s) 232, 605, 655
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 1231
BpmI CTGGAG 1 cut(s) 1098
Bpu10I CCTNAGC 1 cut(s) 1188
Bpu1102I GCTNAGC 1 cut(s) 555
BpuMI CCSGG 3 cut(s) 210, 211, 623
BsaJI CCNNGG 3 cut(s) 120, 209, 622
BsaWI WCCGGW 1 cut(s) 1172
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 1234
Bse118I RCCGGY 1 cut(s) 1172
Bse1I ACTGG 5 cut(s) 139, 416, 888, 929, 1115
Bse3DI GCAATG 2 cut(s) 525, 1069
BseBI CCWGG 4 cut(s) 97, 165, 194, 593
BseDI CCNNGG 3 cut(s) 120, 209, 622
BseGI GGATG 2 cut(s) 202, 284
BseLI CCNNNNNNNGG 2 cut(s) 170, 1234
BseMI GCAATG 2 cut(s) 525, 1069
BseMII CTCAG 2 cut(s) 727, 827
BseNI ACTGG 5 cut(s) 139, 416, 888, 929, 1115
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 112, 488, 872
Bsh1236I CGCG 2 cut(s) 686, 1025
BshFI GGCC 1 cut(s) 591
BshNI GGYRCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 1172
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 3 cut(s) 210, 622, 1173
BslI CCNNNNNNNGG 2 cut(s) 170, 1234
BsnI GGCC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 4 cut(s) 329, 790, 879, 910
Bsp1720I GCTNAGC 1 cut(s) 555
BspACI CCGC 4 cut(s) 310, 325, 588, 686
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 2 cut(s) 726, 826
BspFNI CGCG 2 cut(s) 686, 1025
BspLI GGNNCC 1 cut(s) 175
BspMAI CTGCAG 1 cut(s) 938
BspPI GGATC 4 cut(s) 337, 785, 874, 905
BspQI GCTCTTC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 525, 1069
BsrFI RCCGGY 1 cut(s) 1172
BsrI ACTGG 5 cut(s) 139, 416, 888, 929, 1115
BssAI RCCGGY 1 cut(s) 1172
BssECI CCNNGG 3 cut(s) 120, 209, 622
BssMI GATC 4 cut(s) 329, 790, 879, 910
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 4 cut(s) 97, 165, 194, 593
Bst4CI ACNGT 5 cut(s) 354, 611, 846, 920, 956
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 751
BstC8I GCNNGC 4 cut(s) 545, 561, 586, 743
BstDEI CTNAG 6 cut(s) 374, 555, 659, 713, 813, 1188
BstF5I GGATG 2 cut(s) 202, 284
BstFNI CGCG 2 cut(s) 686, 1025
BstHHI GCGC 2 cut(s) 549, 1027
BstKTI GATC 4 cut(s) 332, 793, 882, 913
BstMBI GATC 4 cut(s) 329, 790, 879, 910
BstMWI GCNNNNNNNGC 4 cut(s) 182, 560, 585, 751
BstNI CCWGG 4 cut(s) 97, 165, 194, 593
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 7 cut(s) 95, 163, 192, 208, 209, 591, 621
BstSFI CTRYAG 1 cut(s) 934
BstUI CGCG 2 cut(s) 686, 1025
BstV1I GCAGC 3 cut(s) 112, 488, 872
BstV2I GAAGAC 1 cut(s) 1231
BstX2I RGATCY 2 cut(s) 879, 910
BstYI RGATCY 2 cut(s) 879, 910
BsuI GTATCC 1 cut(s) 1084
BsuRI GGCC 1 cut(s) 591
BtsCI GGATG 2 cut(s) 202, 284
BtsIMutI CAGTG 2 cut(s) 607, 842
Cac8I GCNNGC 4 cut(s) 545, 561, 586, 743
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 2 cut(s) 549, 1027
Cfr10I RCCGGY 1 cut(s) 1172
Cfr9I CCCGGG 1 cut(s) 209
CseI GACGC 2 cut(s) 692, 714
Csp6I GTAC 2 cut(s) 232, 642
CspAI ACCGGT 1 cut(s) 1172
CviAII CATG 5 cut(s) 544, 873, 947, 1059, 1095
CviQI GTAC 2 cut(s) 232, 642
DdeI CTNAG 6 cut(s) 374, 555, 659, 713, 813, 1188
DpnI GATC 4 cut(s) 331, 792, 881, 912
DpnII GATC 4 cut(s) 329, 790, 879, 910
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 95, 163, 192, 591
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 5 cut(s) 547, 876, 950, 1062, 1098
FatI CATG 5 cut(s) 543, 872, 946, 1058, 1094
FauI CCCGC 1 cut(s) 318
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 5 cut(s) 126, 477, 589, 687, 861
FokI GGATG 2 cut(s) 209, 291
Fsp4HI GCNGC 5 cut(s) 126, 477, 589, 687, 861
FspBI CTAG 2 cut(s) 35, 1142
GlaI GCGC 2 cut(s) 548, 1026
GluI GCNGC 5 cut(s) 126, 477, 589, 687, 861
GsuI CTGGAG 1 cut(s) 1098
HaeIII GGCC 1 cut(s) 591
HapII CCGG 3 cut(s) 210, 622, 1173
HgaI GACGC 2 cut(s) 692, 714
HhaI GCGC 2 cut(s) 549, 1027
Hin1II CATG 5 cut(s) 547, 876, 950, 1062, 1098
Hin6I GCGC 2 cut(s) 547, 1025
HinP1I GCGC 2 cut(s) 547, 1025
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 4 cut(s) 116, 869, 970, 987
HpaII CCGG 3 cut(s) 210, 622, 1173
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 307, 704
Hpy188I TCNGA 4 cut(s) 816, 992, 1033, 1124
Hpy188III TCNNGA 6 cut(s) 240, 398, 636, 680, 715, 788
Hpy8I GTNNAC 2 cut(s) 307, 704
HpyCH4III ACNGT 5 cut(s) 354, 611, 846, 920, 956
HpyCH4IV ACGT 2 cut(s) 203, 502
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 560, 585, 751
HpyF3I CTNAG 6 cut(s) 374, 555, 659, 713, 813, 1188
HpySE526I ACGT 2 cut(s) 203, 502
Hsp92II CATG 5 cut(s) 547, 876, 950, 1062, 1098
HspAI GCGC 2 cut(s) 547, 1025
Kzo9I GATC 4 cut(s) 329, 790, 879, 910
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 5 cut(s) 67, 167, 182, 242, 901
Lsp1109I GCAGC 3 cut(s) 112, 488, 872
LweI GCATC 3 cut(s) 232, 605, 655
MaeI CTAG 2 cut(s) 35, 1142
MaeII ACGT 2 cut(s) 203, 502
MaeIII GTNAC 5 cut(s) 130, 204, 284, 920, 1214
MalI GATC 4 cut(s) 331, 792, 881, 912
MboI GATC 4 cut(s) 329, 790, 879, 910
MboII GAAGA 6 cut(s) 401, 536, 557, 799, 940, 1231
MfeI CAATTG 2 cut(s) 263, 1134
MflI RGATCY 2 cut(s) 879, 910
MluCI AATT 8 cut(s) 263, 298, 338, 718, 1019, 1054, 1108, 1134
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 7 cut(s) 58, 97, 262, 269, 822, 924, 1077
MroXI GAANNNNTTC 1 cut(s) 1019
MseI TTAA 6 cut(s) 78, 780, 821, 834, 941, 1179
MslI CAYNNNNRTG 1 cut(s) 612
MspI CCGG 3 cut(s) 210, 622, 1173
MspR9I CCNGG 7 cut(s) 97, 165, 194, 210, 211, 593, 623
MunI CAATTG 2 cut(s) 263, 1134
MvaI CCWGG 4 cut(s) 97, 165, 194, 593
MvnI CGCG 2 cut(s) 686, 1025
MwoI GCNNNNNNNGC 4 cut(s) 182, 560, 585, 751
NciI CCSGG 3 cut(s) 210, 211, 623
NdeI CATATG 1 cut(s) 865
NdeII GATC 4 cut(s) 329, 790, 879, 910
NlaIII CATG 5 cut(s) 547, 876, 950, 1062, 1098
NlaIV GGNNCC 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 1214
NspI RCATGY 1 cut(s) 547
PaeI GCATGC 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 1019
PfeI GAWTC 4 cut(s) 116, 869, 970, 987
PflMI CCANNNNNTGG 2 cut(s) 170, 1234
PfoI TCCNGGA 1 cut(s) 163
PinAI ACCGGT 1 cut(s) 1172
PkrI GCNGC 5 cut(s) 127, 478, 590, 688, 862
PsiI TTATAA 1 cut(s) 1052
Psp1406I AACGTT 1 cut(s) 203
Psp6I CCWGG 4 cut(s) 95, 163, 192, 591
PspGI CCWGG 4 cut(s) 95, 163, 192, 591
PspN4I GGNNCC 1 cut(s) 175
PstI CTGCAG 1 cut(s) 938
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 879, 910
RsaI GTAC 2 cut(s) 233, 643
RsaNI GTAC 2 cut(s) 232, 642
RseI CAYNNNNRTG 1 cut(s) 612
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 6 cut(s) 78, 780, 821, 834, 941, 1179
SatI GCNGC 5 cut(s) 126, 477, 589, 687, 861
Sau3AI GATC 4 cut(s) 329, 790, 879, 910
ScrFI CCNGG 7 cut(s) 97, 165, 194, 210, 211, 593, 623
SfaNI GCATC 3 cut(s) 232, 605, 655
SfcI CTRYAG 1 cut(s) 934
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 612
SphI GCATGC 1 cut(s) 547
Sse9I AATT 8 cut(s) 263, 298, 338, 718, 1019, 1054, 1108, 1134
SsiI CCGC 4 cut(s) 310, 325, 588, 686
SspMI CTAG 2 cut(s) 35, 1142
StyD4I CCNGG 7 cut(s) 95, 163, 192, 208, 209, 591, 621
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 5 cut(s) 354, 611, 846, 920, 956
TaiI ACGT 2 cut(s) 206, 505
TasI AATT 8 cut(s) 263, 298, 338, 718, 1019, 1054, 1108, 1134
TatI WGTACW 1 cut(s) 641
TauI GCSGC 2 cut(s) 591, 689
TfiI GAWTC 4 cut(s) 116, 869, 970, 987
Tru1I TTAA 6 cut(s) 78, 780, 821, 834, 941, 1179
Tru9I TTAA 6 cut(s) 78, 780, 821, 834, 941, 1179
TscAI CASTG 2 cut(s) 614, 849
TseFI GTSAC 1 cut(s) 1214
TseI GCWGC 3 cut(s) 125, 476, 860
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 4 cut(s) 17, 96, 861, 1047
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 2 cut(s) 614, 849
Van91I CCANNNNNTGG 2 cut(s) 170, 1234
XapI RAATTY 3 cut(s) 338, 718, 1054
XceI RCATGY 1 cut(s) 547
XmaI CCCGGG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 1019
XspI CTAG 2 cut(s) 35, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.