DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2968 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1731 |
| Acc65I | GGTACC | 1 cut(s) | 1556 |
| AccB1I | GGYRCC | 3 cut(s) | 1194, 1426, 1556 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2540 |
| AccBSI | CCGCTC | 1 cut(s) | 1296 |
| AccII | CGCG | 1 cut(s) | 2592 |
| AccIII | TCCGGA | 1 cut(s) | 1068 |
| AciI | CCGC | 5 cut(s) | 894, 1296, 2128, 2481, 2590 |
| AclWI | GGATC | 3 cut(s) | 749, 956, 1525 |
| AcsI | RAATTY | 3 cut(s) | 1075, 1218, 2318 |
| AcuI | CTGAAG | 1 cut(s) | 1253 |
| AcvI | CACGTG | 1 cut(s) | 335 |
| AcyI | GRCGYC | 1 cut(s) | 229 |
| AfaI | GTAC | 3 cut(s) | 1558, 1576, 2630 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 569, 903, 1326, 1511, 2540, 2579 |
| AflII | CTTAAG | 1 cut(s) | 1547 |
| AgeI | ACCGGT | 1 cut(s) | 569 |
| AjnI | CCWGG | 7 cut(s) | 20, 504, 1579, 2024, 2196, 2560, 2932 |
| Alw21I | GWGCWC | 3 cut(s) | 1746, 2049, 2944 |
| Alw26I | GTCTC | 5 cut(s) | 560, 689, 715, 1035, 1296 |
| AlwI | GGATC | 3 cut(s) | 749, 956, 1525 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2222 |
| Ama87I | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| Aor13HI | TCCGGA | 1 cut(s) | 1068 |
| AoxI | GGCC | 5 cut(s) | 891, 1406, 2193, 2587, 2849 |
| ApeKI | GCWGC | 9 cut(s) | 734, 2021, 2212, 2351, 2455, 2495, 2597, 2609, 2612 |
| ApoI | RAATTY | 3 cut(s) | 1075, 1218, 2318 |
| AsiGI | ACCGGT | 1 cut(s) | 569 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1443, 1474 |
| Asp718I | GGTACC | 1 cut(s) | 1556 |
| AspLEI | GCGC | 1 cut(s) | 96 |
| AspS9I | GGNCC | 6 cut(s) | 560, 1406, 2194, 2741, 2813, 2849 |
| AsuC2I | CCSGG | 3 cut(s) | 1327, 2810, 2811 |
| AsuHPI | GGTGA | 6 cut(s) | 605, 737, 875, 929, 1412, 2453 |
| AsuNHI | GCTAGC | 1 cut(s) | 2548 |
| AvaI | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| AvaII | GGWCC | 3 cut(s) | 560, 2741, 2813 |
| AxyI | CCTNAGG | 2 cut(s) | 792, 2817 |
| BanI | GGYRCC | 3 cut(s) | 1194, 1426, 1556 |
| BanII | GRGCYC | 1 cut(s) | 2049 |
| BbrPI | CACGTG | 1 cut(s) | 335 |
| BbsI | GAAGAC | 5 cut(s) | 750, 984, 1016, 2094, 2910 |
| Bbv12I | GWGCWC | 3 cut(s) | 1746, 2049, 2944 |
| BbvCI | CCTCAGC | 1 cut(s) | 474 |
| BbvI | GCAGC | 9 cut(s) | 721, 2033, 2224, 2363, 2467, 2482, 2596, 2599, 2609 |
| BccI | CCATC | 6 cut(s) | 584, 622, 670, 818, 1397, 2573 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 73, 107 |
| BciT130I | CCWGG | 7 cut(s) | 22, 506, 1581, 2026, 2198, 2562, 2934 |
| BciVI | GTATCC | 5 cut(s) | 129, 135, 1219, 2576, 2816 |
| BcnI | CCSGG | 3 cut(s) | 1327, 2810, 2811 |
| BcoDI | GTCTC | 5 cut(s) | 560, 689, 715, 1035, 1296 |
| BfaI | CTAG | 5 cut(s) | 1212, 1434, 2033, 2549, 2778 |
| BfmI | CTRYAG | 1 cut(s) | 735 |
| BfrI | CTTAAG | 1 cut(s) | 1547 |
| BfuI | GTATCC | 5 cut(s) | 129, 135, 1219, 2576, 2816 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1414 |
| BlpI | GCTNAGC | 1 cut(s) | 806 |
| Bme18I | GGWCC | 3 cut(s) | 560, 2741, 2813 |
| BmeT110I | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| BmgT120I | GGNCC | 6 cut(s) | 560, 1406, 2194, 2741, 2813, 2849 |
| BmiI | GGNNCC | 7 cut(s) | 561, 1196, 1428, 1558, 2508, 2742, 2814 |
| BmsI | GCATC | 9 cut(s) | 81, 101, 658, 754, 1108, 2059, 2107, 2155, 2641 |
| BmtI | GCTAGC | 1 cut(s) | 2552 |
| BpiI | GAAGAC | 5 cut(s) | 750, 984, 1016, 2094, 2910 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 80, 112, 1874, 1906 |
| BpmI | CTGGAG | 2 cut(s) | 1542, 2955 |
| Bpu10I | CCTNAGC | 1 cut(s) | 474 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 806 |
| BpuEI | CTTGAG | 3 cut(s) | 1087, 1876, 2873 |
| BpuMI | CCSGG | 3 cut(s) | 1327, 2810, 2811 |
| Bsa29I | ATCGAT | 2 cut(s) | 822, 1593 |
| BsaAI | YACGTR | 2 cut(s) | 335, 1787 |
| BsaBI | GATNNNNATC | 3 cut(s) | 48, 636, 960 |
| BsaHI | GRCGYC | 1 cut(s) | 229 |
| BsaI | GGTCTC | 1 cut(s) | 560 |
| BsaJI | CCNNGG | 6 cut(s) | 684, 898, 1342, 2025, 2196, 2809 |
| BsaWI | WCCGGW | 2 cut(s) | 569, 1068 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 274, 304 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 569, 903, 1326, 1511, 2540, 2579 |
| Bse118I | RCCGGY | 1 cut(s) | 569 |
| Bse1I | ACTGG | 4 cut(s) | 865, 1207, 2216, 2299 |
| Bse21I | CCTNAGG | 2 cut(s) | 792, 2817 |
| Bse3DI | GCAATG | 1 cut(s) | 2484 |
| Bse8I | GATNNNNATC | 3 cut(s) | 48, 636, 960 |
| BseAI | TCCGGA | 1 cut(s) | 1068 |
| BseBI | CCWGG | 7 cut(s) | 22, 506, 1581, 2026, 2198, 2562, 2934 |
| BseCI | ATCGAT | 2 cut(s) | 822, 1593 |
| BseDI | CCNNGG | 6 cut(s) | 684, 898, 1342, 2025, 2196, 2809 |
| BseGI | GGATG | 8 cut(s) | 445, 460, 637, 1113, 1123, 1663, 2167, 2341 |
| BseJI | GATNNNNATC | 3 cut(s) | 48, 636, 960 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 569, 903, 1326, 1511, 2540, 2579 |
| BseMI | GCAATG | 1 cut(s) | 2484 |
| BseNI | ACTGG | 4 cut(s) | 865, 1207, 2216, 2299 |
| BseRI | GAGGAG | 7 cut(s) | 143, 295, 508, 1001, 1843, 2345, 2952 |
| BseXI | GCAGC | 9 cut(s) | 721, 2033, 2224, 2363, 2467, 2482, 2596, 2599, 2609 |
| BseYI | CCCAGC | 2 cut(s) | 1530, 2737 |
| Bsh1236I | CGCG | 1 cut(s) | 2592 |
| BshFI | GGCC | 5 cut(s) | 893, 1408, 2195, 2589, 2851 |
| BshNI | GGYRCC | 3 cut(s) | 1194, 1426, 1556 |
| BshTI | ACCGGT | 1 cut(s) | 569 |
| BshVI | ATCGAT | 2 cut(s) | 822, 1593 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1746, 2049, 2944 |
| BsiHKCI | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| BsiSI | CCGG | 4 cut(s) | 570, 1069, 1326, 2810 |
| BslFI | GGGAC | 3 cut(s) | 284, 2754, 2826 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 569, 903, 1326, 1511, 2540, 2579 |
| BsmAI | GTCTC | 5 cut(s) | 560, 689, 715, 1035, 1296 |
| BsmFI | GGGAC | 3 cut(s) | 284, 2754, 2826 |
| BsmI | GAATGC | 1 cut(s) | 2710 |
| BsnI | GGCC | 5 cut(s) | 893, 1408, 2195, 2589, 2851 |
| Bso31I | GGTCTC | 1 cut(s) | 560 |
| BsoBI | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1746, 2049, 2944 |
| Bsp13I | TCCGGA | 1 cut(s) | 1068 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 806 |
| BspACI | CCGC | 5 cut(s) | 894, 1296, 2128, 2481, 2590 |
| BspANI | GGCC | 5 cut(s) | 893, 1408, 2195, 2589, 2851 |
| BspDI | ATCGAT | 2 cut(s) | 822, 1593 |
| BspEI | TCCGGA | 1 cut(s) | 1068 |
| BspFNI | CGCG | 1 cut(s) | 2592 |
| BspHI | TCATGA | 1 cut(s) | 1290 |
| BspLI | GGNNCC | 7 cut(s) | 561, 1196, 1428, 1558, 2508, 2742, 2814 |
| BspMAI | CTGCAG | 1 cut(s) | 739 |
| BspOI | GCTAGC | 1 cut(s) | 2552 |
| BspPI | GGATC | 3 cut(s) | 749, 956, 1525 |
| BspT107I | GGYRCC | 3 cut(s) | 1194, 1426, 1556 |
| BspTI | CTTAAG | 1 cut(s) | 1547 |
| BspTNI | GGTCTC | 1 cut(s) | 560 |
| BsrBI | CCGCTC | 1 cut(s) | 1296 |
| BsrDI | GCAATG | 1 cut(s) | 2484 |
| BsrFI | RCCGGY | 1 cut(s) | 569 |
| BsrI | ACTGG | 4 cut(s) | 865, 1207, 2216, 2299 |
| BssAI | RCCGGY | 1 cut(s) | 569 |
| BssECI | CCNNGG | 6 cut(s) | 684, 898, 1342, 2025, 2196, 2809 |
| BssNI | GRCGYC | 1 cut(s) | 229 |
| BssT1I | CCWWGG | 2 cut(s) | 898, 1342 |
| Bst2UI | CCWGG | 7 cut(s) | 22, 506, 1581, 2026, 2198, 2562, 2934 |
| Bst4CI | ACNGT | 3 cut(s) | 709, 1741, 2628 |
| Bst6I | CTCTTC | 8 cut(s) | 120, 217, 345, 493, 518, 992, 2322, 2385 |
| BstACI | GRCGYC | 1 cut(s) | 229 |
| BstAFI | CTTAAG | 1 cut(s) | 1547 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2174 |
| BstBAI | YACGTR | 2 cut(s) | 335, 1787 |
| BstC8I | GCNNGC | 5 cut(s) | 404, 2283, 2550, 2587, 2828 |
| BstEII | GGTNACC | 1 cut(s) | 863 |
| BstF5I | GGATG | 8 cut(s) | 445, 460, 637, 1113, 1123, 1663, 2167, 2341 |
| BstFNI | CGCG | 1 cut(s) | 2592 |
| BstHHI | GCGC | 1 cut(s) | 96 |
| BstMAI | GTCTC | 5 cut(s) | 560, 689, 715, 1035, 1296 |
| BstNI | CCWGG | 7 cut(s) | 22, 506, 1581, 2026, 2198, 2562, 2934 |
| BstPI | GGTNACC | 1 cut(s) | 863 |
| BstSFI | CTRYAG | 1 cut(s) | 735 |
| BstUI | CGCG | 1 cut(s) | 2592 |
| BstV1I | GCAGC | 9 cut(s) | 721, 2033, 2224, 2363, 2467, 2482, 2596, 2599, 2609 |
| BstV2I | GAAGAC | 5 cut(s) | 750, 984, 1016, 2094, 2910 |
| BstX2I | RGATCY | 1 cut(s) | 1517 |
| BstYI | RGATCY | 1 cut(s) | 1517 |
| Bsu15I | ATCGAT | 2 cut(s) | 822, 1593 |
| Bsu36I | CCTNAGG | 2 cut(s) | 792, 2817 |
| BsuI | GTATCC | 5 cut(s) | 129, 135, 1219, 2576, 2816 |
| BsuRI | GGCC | 5 cut(s) | 893, 1408, 2195, 2589, 2851 |
| BsuTUI | ATCGAT | 2 cut(s) | 822, 1593 |
| BtgZI | GCGATG | 1 cut(s) | 2001 |
| BtsCI | GGATG | 8 cut(s) | 445, 460, 637, 1113, 1123, 1663, 2167, 2341 |
| BtsI | GCAGTG | 2 cut(s) | 1128, 1618 |
| BtsIMutI | CAGTG | 4 cut(s) | 1128, 1546, 1618, 2553 |
| Cac8I | GCNNGC | 5 cut(s) | 404, 2283, 2550, 2587, 2828 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2222 |
| CciI | TCATGA | 1 cut(s) | 1290 |
| CfoI | GCGC | 1 cut(s) | 96 |
| Cfr10I | RCCGGY | 1 cut(s) | 569 |
| Cfr13I | GGNCC | 6 cut(s) | 560, 1406, 2194, 2741, 2813, 2849 |
| Cfr9I | CCCGGG | 1 cut(s) | 2809 |
| ClaI | ATCGAT | 2 cut(s) | 822, 1593 |
| CseI | GACGC | 1 cut(s) | 237 |
| Csp6I | GTAC | 3 cut(s) | 1557, 1575, 2629 |
| CspAI | ACCGGT | 1 cut(s) | 569 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1999, 2034 |
| CviAII | CATG | 6 cut(s) | 407, 1160, 1291, 2254, 2288, 2602 |
| CviQI | GTAC | 3 cut(s) | 1557, 1575, 2629 |
| DraI | TTTAAA | 2 cut(s) | 841, 2317 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1731 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1731 |
| Eam1104I | CTCTTC | 8 cut(s) | 120, 217, 345, 493, 518, 992, 2322, 2385 |
| EarI | CTCTTC | 8 cut(s) | 120, 217, 345, 493, 518, 992, 2322, 2385 |
| EciI | GGCGGA | 1 cut(s) | 2143 |
| Ecl136II | GAGCTC | 1 cut(s) | 2047 |
| Eco130I | CCWWGG | 2 cut(s) | 898, 1342 |
| Eco24I | GRGCYC | 1 cut(s) | 2049 |
| Eco31I | GGTCTC | 1 cut(s) | 560 |
| Eco32I | GATATC | 3 cut(s) | 1099, 1465, 2446 |
| Eco47I | GGWCC | 3 cut(s) | 560, 2741, 2813 |
| Eco53kI | GAGCTC | 1 cut(s) | 2047 |
| Eco57I | CTGAAG | 1 cut(s) | 1253 |
| Eco72I | CACGTG | 1 cut(s) | 335 |
| Eco81I | CCTNAGG | 2 cut(s) | 792, 2817 |
| Eco88I | CYCGRG | 5 cut(s) | 97, 435, 1874, 2048, 2809 |
| Eco91I | GGTNACC | 1 cut(s) | 863 |
| EcoICRI | GAGCTC | 1 cut(s) | 2047 |
| EcoO109I | RGGNCCY | 4 cut(s) | 560, 2194, 2813, 2849 |
| EcoO65I | GGTNACC | 1 cut(s) | 863 |
| EcoRII | CCWGG | 7 cut(s) | 20, 504, 1579, 2024, 2196, 2560, 2932 |
| EcoRV | GATATC | 3 cut(s) | 1099, 1465, 2446 |
| EcoT14I | CCWWGG | 2 cut(s) | 898, 1342 |
| EcoT38I | GRGCYC | 1 cut(s) | 2049 |
| ErhI | CCWWGG | 2 cut(s) | 898, 1342 |
| FaeI | CATG | 6 cut(s) | 410, 1163, 1294, 2257, 2291, 2605 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 359, 391 |
| FaqI | GGGAC | 3 cut(s) | 284, 2754, 2826 |
| FatI | CATG | 6 cut(s) | 406, 1159, 1290, 2253, 2287, 2601 |
| FokI | GGATG | 8 cut(s) | 452, 467, 644, 1120, 1130, 1670, 2174, 2348 |
| FriOI | GRGCYC | 1 cut(s) | 2049 |
| FspBI | CTAG | 5 cut(s) | 1212, 1434, 2033, 2549, 2778 |
| GlaI | GCGC | 1 cut(s) | 95 |
| GsaI | CCCAGC | 2 cut(s) | 1534, 2741 |
| GsuI | CTGGAG | 2 cut(s) | 1542, 2955 |
| HaeIII | GGCC | 5 cut(s) | 893, 1408, 2195, 2589, 2851 |
| HapII | CCGG | 4 cut(s) | 570, 1069, 1326, 2810 |
| HgaI | GACGC | 1 cut(s) | 237 |
| HhaI | GCGC | 1 cut(s) | 96 |
| Hin1I | GRCGYC | 1 cut(s) | 229 |
| Hin1II | CATG | 6 cut(s) | 410, 1163, 1294, 2257, 2291, 2605 |
| Hin6I | GCGC | 1 cut(s) | 94 |
| HinP1I | GCGC | 1 cut(s) | 94 |
| HincII | GTYRAC | 1 cut(s) | 1039 |
| HindII | GTYRAC | 1 cut(s) | 1039 |
| HindIII | AAGCTT | 2 cut(s) | 803, 1238 |
| HinfI | GANTC | 9 cut(s) | 116, 501, 583, 596, 620, 923, 929, 2402, 2641 |
| HpaII | CCGG | 4 cut(s) | 570, 1069, 1326, 2810 |
| HphI | GGTGA | 6 cut(s) | 605, 737, 875, 929, 1412, 2453 |
| Hpy166II | GTNNAC | 4 cut(s) | 200, 1039, 1126, 2062 |
| Hpy8I | GTNNAC | 4 cut(s) | 200, 1039, 1126, 2062 |
| Hpy99I | CGWCG | 3 cut(s) | 88, 986, 2846 |
| HpyAV | CCTTC | 8 cut(s) | 19, 847, 1629, 2240, 2365, 2840, 2932, 2939 |
| HpyCH4III | ACNGT | 3 cut(s) | 709, 1741, 2628 |
| HpyCH4IV | ACGT | 6 cut(s) | 238, 289, 334, 1786, 2619, 2803 |
| HpySE526I | ACGT | 6 cut(s) | 238, 289, 334, 1786, 2619, 2803 |
| Hsp92I | GRCGYC | 1 cut(s) | 229 |
| Hsp92II | CATG | 6 cut(s) | 410, 1163, 1294, 2257, 2291, 2605 |
| HspAI | GCGC | 1 cut(s) | 94 |
| Kpn2I | TCCGGA | 1 cut(s) | 1068 |
| KpnI | GGTACC | 1 cut(s) | 1560 |
| LmnI | GCTCC | 6 cut(s) | 394, 1147, 2512, 2582, 2762, 2939 |
| Lsp1109I | GCAGC | 9 cut(s) | 721, 2033, 2224, 2363, 2467, 2482, 2596, 2599, 2609 |
| LweI | GCATC | 9 cut(s) | 81, 101, 658, 754, 1108, 2059, 2107, 2155, 2641 |
| MaeI | CTAG | 5 cut(s) | 1212, 1434, 2033, 2549, 2778 |
| MaeII | ACGT | 6 cut(s) | 238, 289, 334, 1786, 2619, 2803 |
| MaeIII | GTNAC | 9 cut(s) | 239, 330, 703, 725, 863, 1639, 2143, 2512, 2719 |
| MbiI | CCGCTC | 1 cut(s) | 1296 |
| MfeI | CAATTG | 1 cut(s) | 2525 |
| MflI | RGATCY | 1 cut(s) | 1517 |
| MhlI | GDGCHC | 3 cut(s) | 1746, 2049, 2944 |
| MlyI | GAGTC | 4 cut(s) | 510, 577, 614, 2396 |
| MroI | TCCGGA | 1 cut(s) | 1068 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1443, 1474 |
| MslI | CAYNNNNRTG | 1 cut(s) | 411 |
| MspA1I | CMGCKG | 1 cut(s) | 701 |
| MspCI | CTTAAG | 1 cut(s) | 1547 |
| MspI | CCGG | 4 cut(s) | 570, 1069, 1326, 2810 |
| MunI | CAATTG | 1 cut(s) | 2525 |
| Mva1269I | GAATGC | 1 cut(s) | 2710 |
| MvaI | CCWGG | 7 cut(s) | 22, 506, 1581, 2026, 2198, 2562, 2934 |
| MvnI | CGCG | 1 cut(s) | 2592 |
| NciI | CCSGG | 3 cut(s) | 1327, 2810, 2811 |
| NheI | GCTAGC | 1 cut(s) | 2548 |
| NlaIII | CATG | 6 cut(s) | 410, 1163, 1294, 2257, 2291, 2605 |
| NlaIV | GGNNCC | 7 cut(s) | 561, 1196, 1428, 1558, 2508, 2742, 2814 |
| NmeAIII | GCCGAG | 1 cut(s) | 257 |
| NmuCI | GTSAC | 6 cut(s) | 239, 330, 725, 863, 1639, 2512 |
| PaeR7I | CTCGAG | 2 cut(s) | 97, 2048 |
| PagI | TCATGA | 1 cut(s) | 1290 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 43, 235, 981, 1729 |
| PctI | GAATGC | 1 cut(s) | 2710 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1443, 1474 |
| PfeI | GAWTC | 5 cut(s) | 116, 596, 923, 929, 2641 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2540 |
| PfoI | TCCNGGA | 4 cut(s) | 20, 504, 1579, 2932 |
| PinAI | ACCGGT | 1 cut(s) | 569 |
| PleI | GAGTC | 4 cut(s) | 509, 577, 614, 2396 |
| PmaCI | CACGTG | 1 cut(s) | 335 |
| PmlI | CACGTG | 1 cut(s) | 335 |
| PpsI | GAGTC | 4 cut(s) | 509, 577, 614, 2396 |
| Ppu21I | YACGTR | 2 cut(s) | 335, 1787 |
| PpuMI | RGGWCCY | 2 cut(s) | 560, 2813 |
| PsiI | TTATAA | 1 cut(s) | 2968 |
| Psp124BI | GAGCTC | 1 cut(s) | 2049 |
| Psp5II | RGGWCCY | 2 cut(s) | 560, 2813 |
| Psp6I | CCWGG | 7 cut(s) | 20, 504, 1579, 2024, 2196, 2560, 2932 |
| PspCI | CACGTG | 1 cut(s) | 335 |
| PspEI | GGTNACC | 1 cut(s) | 863 |
| PspFI | CCCAGC | 2 cut(s) | 1530, 2737 |
| PspGI | CCWGG | 7 cut(s) | 20, 504, 1579, 2024, 2196, 2560, 2932 |
| PspN4I | GGNNCC | 7 cut(s) | 561, 1196, 1428, 1558, 2508, 2742, 2814 |
| PspPI | GGNCC | 6 cut(s) | 560, 1406, 2194, 2741, 2813, 2849 |
| PspPPI | RGGWCCY | 2 cut(s) | 560, 2813 |
| PspXI | VCTCGAGB | 1 cut(s) | 2048 |
| PstI | CTGCAG | 1 cut(s) | 739 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2222 |
| PsuI | RGATCY | 1 cut(s) | 1517 |
| PvuII | CAGCTG | 1 cut(s) | 701 |
| RsaI | GTAC | 3 cut(s) | 1558, 1576, 2630 |
| RsaNI | GTAC | 3 cut(s) | 1557, 1575, 2629 |
| RseI | CAYNNNNRTG | 1 cut(s) | 411 |
| SacI | GAGCTC | 1 cut(s) | 2049 |
| Sau96I | GGNCC | 6 cut(s) | 560, 1406, 2194, 2741, 2813, 2849 |
| SchI | GAGTC | 4 cut(s) | 510, 577, 614, 2396 |
| SduI | GDGCHC | 3 cut(s) | 1746, 2049, 2944 |
| SfaNI | GCATC | 9 cut(s) | 81, 101, 658, 754, 1108, 2059, 2107, 2155, 2641 |
| SfcI | CTRYAG | 1 cut(s) | 735 |
| Sfr274I | CTCGAG | 2 cut(s) | 97, 2048 |
| SinI | GGWCC | 3 cut(s) | 560, 2741, 2813 |
| SlaI | CTCGAG | 2 cut(s) | 97, 2048 |
| SmaI | CCCGGG | 1 cut(s) | 2811 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 411 |
| SmlI | CTYRAG | 6 cut(s) | 97, 1102, 1547, 1891, 2048, 2888 |
| SmoI | CTYRAG | 6 cut(s) | 97, 1102, 1547, 1891, 2048, 2888 |
| SsiI | CCGC | 5 cut(s) | 894, 1296, 2128, 2481, 2590 |
| SspI | AATATT | 2 cut(s) | 814, 1720 |
| SspMI | CTAG | 5 cut(s) | 1212, 1434, 2033, 2549, 2778 |
| SstI | GAGCTC | 1 cut(s) | 2049 |
| StyI | CCWWGG | 2 cut(s) | 898, 1342 |
| TaaI | ACNGT | 3 cut(s) | 709, 1741, 2628 |
| TaiI | ACGT | 6 cut(s) | 241, 292, 337, 1789, 2622, 2806 |
| TaqI | TCGA | 9 cut(s) | 98, 654, 822, 1086, 1593, 1723, 2049, 2067, 2844 |
| TatI | WGTACW | 1 cut(s) | 1574 |
| TauI | GCSGC | 3 cut(s) | 896, 2483, 2592 |
| TfiI | GAWTC | 5 cut(s) | 116, 596, 923, 929, 2641 |
| TscAI | CASTG | 4 cut(s) | 1128, 1546, 1618, 2560 |
| TseFI | GTSAC | 6 cut(s) | 239, 330, 725, 863, 1639, 2512 |
| TseI | GCWGC | 9 cut(s) | 734, 2021, 2212, 2351, 2455, 2495, 2597, 2609, 2612 |
| Tsp45I | GTSAC | 6 cut(s) | 239, 330, 725, 863, 1639, 2512 |
| TspGWI | ACGGA | 5 cut(s) | 101, 154, 160, 407, 505 |
| TspMI | CCCGGG | 1 cut(s) | 2809 |
| TspRI | CASTG | 4 cut(s) | 1128, 1546, 1618, 2560 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2540 |
| Vha464I | CTTAAG | 1 cut(s) | 1547 |
| VpaK11BI | GGWCC | 3 cut(s) | 560, 2741, 2813 |
| XapI | RAATTY | 3 cut(s) | 1075, 1218, 2318 |
| XbaI | TCTAGA | 1 cut(s) | 2032 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2751 |
| XhoI | CTCGAG | 2 cut(s) | 97, 2048 |
| XmaI | CCCGGG | 1 cut(s) | 2809 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1443, 1474 |
| XspI | CTAG | 5 cut(s) | 1212, 1434, 2033, 2549, 2778 |