DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1124, 2011 |
| AccB1I | GGYRCC | 2 cut(s) | 1446, 1678 |
| AccBSI | CCGCTC | 2 cut(s) | 340, 2690 |
| AccI | GTMKAC | 1 cut(s) | 1761 |
| AccIII | TCCGGA | 1 cut(s) | 824 |
| AciI | CCGC | 7 cut(s) | 340, 529, 727, 1804, 2076, 2690, 3017 |
| AclI | AACGTT | 1 cut(s) | 3146 |
| AclWI | GGATC | 6 cut(s) | 72, 78, 992, 1208, 1588, 3046 |
| AcoI | YGGCCR | 2 cut(s) | 2455, 2464 |
| AcsI | RAATTY | 4 cut(s) | 1327, 2187, 2401, 2657 |
| AcuI | CTGAAG | 1 cut(s) | 1820 |
| AcyI | GRCGYC | 1 cut(s) | 2916 |
| AfaI | GTAC | 6 cut(s) | 182, 554, 1605, 1828, 2202, 2452 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 679, 1639, 1763, 2020, 2891, 2918, 2966, 2981, 3227 |
| AflII | CTTAAG | 1 cut(s) | 2243 |
| AgeI | ACCGGT | 1 cut(s) | 1639 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 820, 2900 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2055, 2087 |
| Alw26I | GTCTC | 5 cut(s) | 875, 1548, 1748, 2267, 2497 |
| AlwI | GGATC | 6 cut(s) | 72, 78, 992, 1208, 1588, 3046 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 977, 1531 |
| Ama87I | CYCGRG | 1 cut(s) | 3000 |
| Aor13HI | TCCGGA | 1 cut(s) | 824 |
| AoxI | GGCC | 7 cut(s) | 1658, 2277, 2455, 2464, 2706, 3151, 3259 |
| ApaI | GGGCCC | 2 cut(s) | 3155, 3263 |
| ApoI | RAATTY | 4 cut(s) | 1327, 2187, 2401, 2657 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1922, 1954, 1960, 1992, 3261, 3293 |
| AseI | ATTAAT | 1 cut(s) | 963 |
| AsiGI | ACCGGT | 1 cut(s) | 1639 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2763 |
| AspS9I | GGNCC | 9 cut(s) | 1195, 1630, 1658, 2277, 3151, 3152, 3224, 3259, 3260 |
| AsuHPI | GGTGA | 8 cut(s) | 1039, 1130, 1267, 1354, 2407, 2429, 2792, 3124 |
| AsuII | TTCGAA | 1 cut(s) | 2767 |
| AvaI | CYCGRG | 1 cut(s) | 3000 |
| AvaII | GGWCC | 3 cut(s) | 1195, 1630, 3224 |
| BaeGI | GKGCMC | 2 cut(s) | 3155, 3263 |
| BanI | GGYRCC | 2 cut(s) | 1446, 1678 |
| BanII | GRGCYC | 3 cut(s) | 623, 3155, 3263 |
| BbsI | GAAGAC | 1 cut(s) | 2736 |
| BbvCI | CCTCAGC | 2 cut(s) | 1127, 1616 |
| BccI | CCATC | 7 cut(s) | 602, 839, 923, 956, 1649, 2984, 3117 |
| BceAI | ACGGC | 1 cut(s) | 2442 |
| BcoDI | GTCTC | 5 cut(s) | 875, 1548, 1748, 2267, 2497 |
| BfaI | CTAG | 5 cut(s) | 24, 2364, 2372, 2508, 3120 |
| BfmI | CTRYAG | 3 cut(s) | 978, 2844, 3006 |
| BfrI | CTTAAG | 1 cut(s) | 2243 |
| BfuAI | ACCTGC | 2 cut(s) | 1124, 2011 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 1666, 2463, 2472 |
| BglII | AGATCT | 4 cut(s) | 1350, 2236, 2521, 3318 |
| Bme18I | GGWCC | 3 cut(s) | 1195, 1630, 3224 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 820, 2900 |
| BmeT110I | CYCGRG | 1 cut(s) | 3000 |
| BmgT120I | GGNCC | 9 cut(s) | 1195, 1630, 1658, 2277, 3151, 3152, 3224, 3259, 3260 |
| BmiI | GGNNCC | 7 cut(s) | 1196, 1448, 1680, 2278, 3153, 3219, 3261 |
| BmsI | GCATC | 5 cut(s) | 934, 1020, 2212, 2494, 3052 |
| BpiI | GAAGAC | 1 cut(s) | 2736 |
| BpmI | CTGGAG | 3 cut(s) | 2502, 3192, 3366 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1127, 1524, 1616 |
| Bpu14I | TTCGAA | 1 cut(s) | 2767 |
| BpuEI | CTTGAG | 3 cut(s) | 1220, 1339, 2164 |
| BsaAI | YACGTR | 1 cut(s) | 184 |
| BsaBI | GATNNNNATC | 3 cut(s) | 63, 69, 1212 |
| BsaHI | GRCGYC | 1 cut(s) | 2916 |
| BsaI | GGTCTC | 1 cut(s) | 1748 |
| BsaJI | CCNNGG | 9 cut(s) | 1166, 1686, 1731, 2015, 2626, 2960, 3189, 3209, 3343 |
| BsaWI | WCCGGW | 3 cut(s) | 824, 1386, 1639 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 568, 598, 924, 954 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 679, 1639, 1763, 2020, 2891, 2918, 2966, 2981, 3227 |
| Bse118I | RCCGGY | 1 cut(s) | 1639 |
| Bse1I | ACTGG | 8 cut(s) | 1459, 1582, 2352, 2397, 2458, 2638, 2971, 3175 |
| Bse3DI | GCAATG | 3 cut(s) | 2332, 2856, 3072 |
| Bse8I | GATNNNNATC | 3 cut(s) | 63, 69, 1212 |
| BseAI | TCCGGA | 1 cut(s) | 824 |
| BseDI | CCNNGG | 9 cut(s) | 1166, 1686, 1731, 2015, 2626, 2960, 3189, 3209, 3343 |
| BseJI | GATNNNNATC | 3 cut(s) | 63, 69, 1212 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 679, 1639, 1763, 2020, 2891, 2918, 2966, 2981, 3227 |
| BseMI | GCAATG | 3 cut(s) | 2332, 2856, 3072 |
| BseNI | ACTGG | 8 cut(s) | 1459, 1582, 2352, 2397, 2458, 2638, 2971, 3175 |
| BseRI | GAGGAG | 9 cut(s) | 61, 132, 188, 463, 646, 692, 695, 745, 748 |
| BseSI | GKGCMC | 2 cut(s) | 3155, 3263 |
| BseYI | CCCAGC | 1 cut(s) | 1649 |
| BsgI | GTGCAG | 1 cut(s) | 1830 |
| BshFI | GGCC | 7 cut(s) | 1660, 2279, 2457, 2466, 2708, 3153, 3261 |
| BshNI | GGYRCC | 2 cut(s) | 1446, 1678 |
| BshTI | ACCGGT | 1 cut(s) | 1639 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3000 |
| BsiSI | CCGG | 4 cut(s) | 825, 1387, 1640, 2467 |
| BslFI | GGGAC | 6 cut(s) | 299, 1208, 2396, 2625, 2644, 2977 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 679, 1639, 1763, 2020, 2891, 2918, 2966, 2981, 3227 |
| BsmAI | GTCTC | 5 cut(s) | 875, 1548, 1748, 2267, 2497 |
| BsmFI | GGGAC | 6 cut(s) | 299, 1208, 2396, 2625, 2644, 2977 |
| BsmI | GAATGC | 2 cut(s) | 1057, 3121 |
| BsnI | GGCC | 7 cut(s) | 1660, 2279, 2457, 2466, 2708, 3153, 3261 |
| Bso31I | GGTCTC | 1 cut(s) | 1748 |
| BsoBI | CYCGRG | 1 cut(s) | 3000 |
| Bsp119I | TTCGAA | 1 cut(s) | 2767 |
| Bsp120I | GGGCCC | 2 cut(s) | 3151, 3259 |
| Bsp1286I | GDGCHC | 3 cut(s) | 623, 3155, 3263 |
| Bsp13I | TCCGGA | 1 cut(s) | 824 |
| Bsp1407I | TGTACA | 1 cut(s) | 2200 |
| Bsp19I | CCATGG | 1 cut(s) | 2626 |
| BspACI | CCGC | 7 cut(s) | 340, 529, 727, 1804, 2076, 2690, 3017 |
| BspANI | GGCC | 7 cut(s) | 1660, 2279, 2457, 2466, 2708, 3153, 3261 |
| BspEI | TCCGGA | 1 cut(s) | 824 |
| BspLI | GGNNCC | 7 cut(s) | 1196, 1448, 1680, 2278, 3153, 3219, 3261 |
| BspMAI | CTGCAG | 2 cut(s) | 982, 3010 |
| BspMI | ACCTGC | 2 cut(s) | 1124, 2011 |
| BspPI | GGATC | 6 cut(s) | 72, 78, 992, 1208, 1588, 3046 |
| BspQI | GCTCTTC | 1 cut(s) | 2913 |
| BspT104I | TTCGAA | 1 cut(s) | 2767 |
| BspT107I | GGYRCC | 2 cut(s) | 1446, 1678 |
| BspTI | CTTAAG | 1 cut(s) | 2243 |
| BspTNI | GGTCTC | 1 cut(s) | 1748 |
| BsrBI | CCGCTC | 2 cut(s) | 340, 2690 |
| BsrDI | GCAATG | 3 cut(s) | 2332, 2856, 3072 |
| BsrFI | RCCGGY | 1 cut(s) | 1639 |
| BsrGI | TGTACA | 1 cut(s) | 2200 |
| BsrI | ACTGG | 8 cut(s) | 1459, 1582, 2352, 2397, 2458, 2638, 2971, 3175 |
| BssAI | RCCGGY | 1 cut(s) | 1639 |
| BssECI | CCNNGG | 9 cut(s) | 1166, 1686, 1731, 2015, 2626, 2960, 3189, 3209, 3343 |
| BssNAI | GTATAC | 1 cut(s) | 1762 |
| BssNI | GRCGYC | 1 cut(s) | 2916 |
| BssT1I | CCWWGG | 2 cut(s) | 1731, 2626 |
| Bst1107I | GTATAC | 1 cut(s) | 1762 |
| Bst4CI | ACNGT | 6 cut(s) | 1060, 1712, 1993, 2441, 2891, 3273 |
| Bst6I | CTCTTC | 6 cut(s) | 326, 393, 410, 533, 2913, 3309 |
| BstACI | GRCGYC | 1 cut(s) | 2916 |
| BstAFI | CTTAAG | 1 cut(s) | 2243 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 3014 |
| BstAUI | TGTACA | 1 cut(s) | 2200 |
| BstBAI | YACGTR | 1 cut(s) | 184 |
| BstBI | TTCGAA | 1 cut(s) | 2767 |
| BstC8I | GCNNGC | 5 cut(s) | 871, 1445, 2464, 2796, 3239 |
| BstDSI | CCRYGG | 1 cut(s) | 2626 |
| BstMAI | GTCTC | 5 cut(s) | 875, 1548, 1748, 2267, 2497 |
| BstSFI | CTRYAG | 3 cut(s) | 978, 2844, 3006 |
| BstSLI | GKGCMC | 2 cut(s) | 3155, 3263 |
| BstV2I | GAAGAC | 1 cut(s) | 2736 |
| BstX2I | RGATCY | 5 cut(s) | 1350, 2236, 2521, 3051, 3318 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1738, 2633 |
| BstYI | RGATCY | 5 cut(s) | 1350, 2236, 2521, 3051, 3318 |
| BstZ17I | GTATAC | 1 cut(s) | 1762 |
| BsuRI | GGCC | 7 cut(s) | 1660, 2279, 2457, 2466, 2708, 3153, 3261 |
| BtgI | CCRYGG | 1 cut(s) | 2626 |
| BtsI | GCAGTG | 2 cut(s) | 1529, 2992 |
| BtsIMutI | CAGTG | 4 cut(s) | 1529, 1717, 2359, 2992 |
| BveI | ACCTGC | 2 cut(s) | 1124, 2011 |
| Cac8I | GCNNGC | 5 cut(s) | 871, 1445, 2464, 2796, 3239 |
| CaiI | CAGNNNCTG | 2 cut(s) | 977, 1531 |
| Cfr10I | RCCGGY | 1 cut(s) | 1639 |
| Cfr13I | GGNCC | 9 cut(s) | 1195, 1630, 1658, 2277, 3151, 3152, 3224, 3259, 3260 |
| CseI | GACGC | 1 cut(s) | 2905 |
| Csp6I | GTAC | 6 cut(s) | 181, 553, 1604, 1827, 2201, 2451 |
| CspAI | ACCGGT | 1 cut(s) | 1639 |
| CviAII | CATG | 9 cut(s) | 1412, 1906, 1933, 2104, 2132, 2249, 2273, 2627, 3025 |
| CviQI | GTAC | 6 cut(s) | 181, 553, 1604, 1827, 2201, 2451 |
| DraI | TTTAAA | 1 cut(s) | 2656 |
| DriI | GACNNNNNGTC | 2 cut(s) | 820, 2900 |
| EaeI | YGGCCR | 2 cut(s) | 2455, 2464 |
| Eam1104I | CTCTTC | 6 cut(s) | 326, 393, 410, 533, 2913, 3309 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 820, 2900 |
| EarI | CTCTTC | 6 cut(s) | 326, 393, 410, 533, 2913, 3309 |
| EciI | GGCGGA | 1 cut(s) | 2091 |
| Eco130I | CCWWGG | 2 cut(s) | 1731, 2626 |
| Eco24I | GRGCYC | 3 cut(s) | 623, 3155, 3263 |
| Eco31I | GGTCTC | 1 cut(s) | 1748 |
| Eco32I | GATATC | 4 cut(s) | 1717, 2255, 2785, 3368 |
| Eco47I | GGWCC | 3 cut(s) | 1195, 1630, 3224 |
| Eco57I | CTGAAG | 1 cut(s) | 1820 |
| Eco88I | CYCGRG | 1 cut(s) | 3000 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1195, 3152, 3259, 3260 |
| EcoRV | GATATC | 4 cut(s) | 1717, 2255, 2785, 3368 |
| EcoT14I | CCWWGG | 2 cut(s) | 1731, 2626 |
| EcoT38I | GRGCYC | 3 cut(s) | 623, 3155, 3263 |
| ErhI | CCWWGG | 2 cut(s) | 1731, 2626 |
| FaeI | CATG | 9 cut(s) | 1415, 1909, 1936, 2107, 2135, 2252, 2276, 2630, 3028 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 3230, 3262 |
| FaqI | GGGAC | 6 cut(s) | 299, 1208, 2396, 2625, 2644, 2977 |
| FatI | CATG | 9 cut(s) | 1411, 1905, 1932, 2103, 2131, 2248, 2272, 2626, 3024 |
| FauI | CCCGC | 1 cut(s) | 1797 |
| FblI | GTMKAC | 1 cut(s) | 1761 |
| FriOI | GRGCYC | 3 cut(s) | 623, 3155, 3263 |
| FspBI | CTAG | 5 cut(s) | 24, 2364, 2372, 2508, 3120 |
| GsaI | CCCAGC | 1 cut(s) | 1653 |
| GsuI | CTGGAG | 3 cut(s) | 2502, 3192, 3366 |
| HaeIII | GGCC | 7 cut(s) | 1660, 2279, 2457, 2466, 2708, 3153, 3261 |
| HapII | CCGG | 4 cut(s) | 825, 1387, 1640, 2467 |
| HgaI | GACGC | 1 cut(s) | 2905 |
| Hin1I | GRCGYC | 1 cut(s) | 2916 |
| Hin1II | CATG | 9 cut(s) | 1415, 1909, 1936, 2107, 2135, 2252, 2276, 2630, 3028 |
| HincII | GTYRAC | 1 cut(s) | 1291 |
| HindII | GTYRAC | 1 cut(s) | 1291 |
| HindIII | AAGCTT | 1 cut(s) | 1008 |
| HpaII | CCGG | 4 cut(s) | 825, 1387, 1640, 2467 |
| HphI | GGTGA | 8 cut(s) | 1039, 1130, 1267, 1354, 2407, 2429, 2792, 3124 |
| Hpy166II | GTNNAC | 7 cut(s) | 1291, 1762, 1796, 2304, 2437, 2894, 3168 |
| Hpy8I | GTNNAC | 7 cut(s) | 1291, 1762, 1796, 2304, 2437, 2894, 3168 |
| Hpy99I | CGWCG | 2 cut(s) | 144, 2921 |
| HpyCH4III | ACNGT | 6 cut(s) | 1060, 1712, 1993, 2441, 2891, 3273 |
| HpyCH4IV | ACGT | 5 cut(s) | 183, 382, 894, 2449, 3146 |
| HpySE526I | ACGT | 5 cut(s) | 183, 382, 894, 2449, 3146 |
| Hsp92I | GRCGYC | 1 cut(s) | 2916 |
| Hsp92II | CATG | 9 cut(s) | 1415, 1909, 1936, 2107, 2135, 2252, 2276, 2630, 3028 |
| Kpn2I | TCCGGA | 1 cut(s) | 824 |
| LguI | GCTCTTC | 1 cut(s) | 2913 |
| LmnI | GCTCC | 6 cut(s) | 337, 514, 1690, 2383, 2521, 3173 |
| LweI | GCATC | 5 cut(s) | 934, 1020, 2212, 2494, 3052 |
| MaeI | CTAG | 5 cut(s) | 24, 2364, 2372, 2508, 3120 |
| MaeII | ACGT | 5 cut(s) | 183, 382, 894, 2449, 3146 |
| MaeIII | GTNAC | 4 cut(s) | 131, 1891, 2157, 3130 |
| MbiI | CCGCTC | 2 cut(s) | 340, 2690 |
| MfeI | CAATTG | 3 cut(s) | 1071, 1394, 2108 |
| MflI | RGATCY | 5 cut(s) | 1350, 2236, 2521, 3051, 3318 |
| MhlI | GDGCHC | 3 cut(s) | 623, 3155, 3263 |
| MlyI | GAGTC | 7 cut(s) | 58, 136, 324, 832, 1175, 2309, 2851 |
| MmeI | TCCRAC | 3 cut(s) | 1549, 2425, 3072 |
| MroI | TCCGGA | 1 cut(s) | 824 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2763 |
| MseI | TTAA | 7 cut(s) | 758, 963, 2244, 2319, 2655, 2661, 3299 |
| MslI | CAYNNNNRTG | 4 cut(s) | 477, 603, 1541, 3060 |
| MspA1I | CMGCKG | 1 cut(s) | 974 |
| MspCI | CTTAAG | 1 cut(s) | 2243 |
| MspI | CCGG | 4 cut(s) | 825, 1387, 1640, 2467 |
| MunI | CAATTG | 3 cut(s) | 1071, 1394, 2108 |
| Mva1269I | GAATGC | 2 cut(s) | 1057, 3121 |
| NcoI | CCATGG | 1 cut(s) | 2626 |
| NlaIII | CATG | 9 cut(s) | 1415, 1909, 1936, 2107, 2135, 2252, 2276, 2630, 3028 |
| NlaIV | GGNNCC | 7 cut(s) | 1196, 1448, 1680, 2278, 3153, 3219, 3261 |
| NmeAIII | GCCGAG | 1 cut(s) | 1996 |
| NmuCI | GTSAC | 2 cut(s) | 131, 3130 |
| NspV | TTCGAA | 1 cut(s) | 2767 |
| PciSI | GCTCTTC | 1 cut(s) | 2913 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 136, 148, 2455 |
| PctI | GAATGC | 2 cut(s) | 1057, 3121 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2763 |
| PfeI | GAWTC | 7 cut(s) | 167, 494, 647, 851, 1175, 1705, 2741 |
| PfoI | TCCNGGA | 1 cut(s) | 3220 |
| PinAI | ACCGGT | 1 cut(s) | 1639 |
| PleI | GAGTC | 7 cut(s) | 57, 135, 323, 832, 1175, 2308, 2851 |
| PpsI | GAGTC | 7 cut(s) | 57, 135, 323, 832, 1175, 2308, 2851 |
| Ppu21I | YACGTR | 1 cut(s) | 184 |
| PpuMI | RGGWCCY | 1 cut(s) | 1195 |
| PshBI | ATTAAT | 1 cut(s) | 963 |
| Psp1406I | AACGTT | 1 cut(s) | 3146 |
| Psp5II | RGGWCCY | 1 cut(s) | 1195 |
| PspFI | CCCAGC | 1 cut(s) | 1649 |
| PspN4I | GGNNCC | 7 cut(s) | 1196, 1448, 1680, 2278, 3153, 3219, 3261 |
| PspOMI | GGGCCC | 2 cut(s) | 3151, 3259 |
| PspPI | GGNCC | 9 cut(s) | 1195, 1630, 1658, 2277, 3151, 3152, 3224, 3259, 3260 |
| PspPPI | RGGWCCY | 1 cut(s) | 1195 |
| PstI | CTGCAG | 2 cut(s) | 982, 3010 |
| PstNI | CAGNNNCTG | 2 cut(s) | 977, 1531 |
| PsuI | RGATCY | 5 cut(s) | 1350, 2236, 2521, 3051, 3318 |
| PvuII | CAGCTG | 1 cut(s) | 974 |
| RsaI | GTAC | 6 cut(s) | 182, 554, 1605, 1828, 2202, 2452 |
| RsaNI | GTAC | 6 cut(s) | 181, 553, 1604, 1827, 2201, 2451 |
| RseI | CAYNNNNRTG | 4 cut(s) | 477, 603, 1541, 3060 |
| SapI | GCTCTTC | 1 cut(s) | 2913 |
| SaqAI | TTAA | 7 cut(s) | 758, 963, 2244, 2319, 2655, 2661, 3299 |
| Sau96I | GGNCC | 9 cut(s) | 1195, 1630, 1658, 2277, 3151, 3152, 3224, 3259, 3260 |
| SchI | GAGTC | 7 cut(s) | 58, 136, 324, 832, 1175, 2309, 2851 |
| SduI | GDGCHC | 3 cut(s) | 623, 3155, 3263 |
| SfaNI | GCATC | 5 cut(s) | 934, 1020, 2212, 2494, 3052 |
| SfcI | CTRYAG | 3 cut(s) | 978, 2844, 3006 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2463 |
| SfuI | TTCGAA | 1 cut(s) | 2767 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1639 |
| SinI | GGWCC | 3 cut(s) | 1195, 1630, 3224 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 477, 603, 1541, 3060 |
| SmlI | CTYRAG | 4 cut(s) | 1199, 1354, 2143, 2243 |
| SmoI | CTYRAG | 4 cut(s) | 1199, 1354, 2143, 2243 |
| SsiI | CCGC | 7 cut(s) | 340, 529, 727, 1804, 2076, 2690, 3017 |
| SspI | AATATT | 1 cut(s) | 1972 |
| SspMI | CTAG | 5 cut(s) | 24, 2364, 2372, 2508, 3120 |
| StyI | CCWWGG | 2 cut(s) | 1731, 2626 |
| TaaI | ACNGT | 6 cut(s) | 1060, 1712, 1993, 2441, 2891, 3273 |
| TaiI | ACGT | 5 cut(s) | 186, 385, 897, 2452, 3149 |
| TatI | WGTACW | 4 cut(s) | 552, 1603, 1826, 2200 |
| TauI | GCSGC | 3 cut(s) | 532, 2693, 3020 |
| TfiI | GAWTC | 7 cut(s) | 167, 494, 647, 851, 1175, 1705, 2741 |
| Tru1I | TTAA | 7 cut(s) | 758, 963, 2244, 2319, 2655, 2661, 3299 |
| Tru9I | TTAA | 7 cut(s) | 758, 963, 2244, 2319, 2655, 2661, 3299 |
| TscAI | CASTG | 4 cut(s) | 1536, 1717, 2359, 2992 |
| TseFI | GTSAC | 2 cut(s) | 131, 3130 |
| Tsp45I | GTSAC | 2 cut(s) | 131, 3130 |
| TspGWI | ACGGA | 3 cut(s) | 715, 1310, 1779 |
| TspRI | CASTG | 4 cut(s) | 1536, 1717, 2359, 2992 |
| Vha464I | CTTAAG | 1 cut(s) | 2243 |
| VpaK11BI | GGWCC | 3 cut(s) | 1195, 1630, 3224 |
| VspI | ATTAAT | 1 cut(s) | 963 |
| XapI | RAATTY | 4 cut(s) | 1327, 2187, 2401, 2657 |
| XmiI | GTMKAC | 1 cut(s) | 1761 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2763 |
| XspI | CTAG | 5 cut(s) | 24, 2364, 2372, 2508, 3120 |