DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 1352, 2239 |
| AccB1I | GGYRCC | 3 cut(s) | 21, 1674, 1906 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 3146 |
| AccBSI | CCGCTC | 3 cut(s) | 295, 562, 2918 |
| AccI | GTMKAC | 1 cut(s) | 1989 |
| AciI | CCGC | 9 cut(s) | 190, 295, 562, 757, 955, 2032, 2304, 2918, 3245 |
| AclI | AACGTT | 1 cut(s) | 3374 |
| AclWI | GGATC | 6 cut(s) | 116, 306, 1220, 1436, 1816, 3274 |
| AcoI | YGGCCR | 2 cut(s) | 2683, 2692 |
| AcsI | RAATTY | 5 cut(s) | 110, 1555, 2415, 2629, 2885 |
| AcuI | CTGAAG | 2 cut(s) | 170, 2048 |
| AfaI | GTAC | 6 cut(s) | 410, 782, 1833, 2056, 2430, 2680 |
| AflII | CTTAAG | 2 cut(s) | 737, 2471 |
| AgeI | ACCGGT | 1 cut(s) | 24 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 1048, 3128 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 164, 196, 2283, 2315 |
| Alw26I | GTCTC | 4 cut(s) | 1776, 1976, 2495, 2725 |
| AlwI | GGATC | 6 cut(s) | 116, 306, 1220, 1436, 1816, 3274 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1205, 1759 |
| Ama87I | CYCGRG | 1 cut(s) | 3228 |
| AoxI | GGCC | 6 cut(s) | 1886, 2505, 2683, 2692, 3379, 3487 |
| ApaI | GGGCCC | 2 cut(s) | 3383, 3491 |
| ApoI | RAATTY | 5 cut(s) | 110, 1555, 2415, 2629, 2885 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 2150, 2182, 2188, 2220, 3489, 3521 |
| AsiGI | ACCGGT | 1 cut(s) | 24 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2991 |
| AspLEI | GCGC | 2 cut(s) | 142, 158 |
| AspS9I | GGNCC | 9 cut(s) | 1423, 1858, 1886, 2505, 3379, 3380, 3452, 3487, 3488 |
| AsuHPI | GGTGA | 7 cut(s) | 1267, 1358, 1582, 2635, 2657, 3020, 3352 |
| AsuII | TTCGAA | 1 cut(s) | 2995 |
| AvaI | CYCGRG | 1 cut(s) | 3228 |
| AvaII | GGWCC | 3 cut(s) | 1423, 1858, 3452 |
| BaeGI | GKGCMC | 2 cut(s) | 3383, 3491 |
| BanI | GGYRCC | 3 cut(s) | 21, 1674, 1906 |
| BanII | GRGCYC | 3 cut(s) | 851, 3383, 3491 |
| BbsI | GAAGAC | 1 cut(s) | 2964 |
| BbvCI | CCTCAGC | 2 cut(s) | 1355, 1844 |
| BccI | CCATC | 7 cut(s) | 830, 1067, 1151, 1184, 1877, 3212, 3345 |
| BceAI | ACGGC | 1 cut(s) | 2670 |
| BcoDI | GTCTC | 4 cut(s) | 1776, 1976, 2495, 2725 |
| BfaI | CTAG | 6 cut(s) | 209, 252, 2592, 2600, 2736, 3348 |
| BfmI | CTRYAG | 3 cut(s) | 1206, 3072, 3234 |
| BfoI | RGCGCY | 2 cut(s) | 143, 159 |
| BfrI | CTTAAG | 2 cut(s) | 737, 2471 |
| BfuAI | ACCTGC | 2 cut(s) | 1352, 2239 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 1894, 2691, 2700 |
| BglII | AGATCT | 4 cut(s) | 1578, 2464, 2749, 3546 |
| Bme18I | GGWCC | 3 cut(s) | 1423, 1858, 3452 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 1048, 3128 |
| BmeT110I | CYCGRG | 1 cut(s) | 3228 |
| BmgT120I | GGNCC | 9 cut(s) | 1423, 1858, 1886, 2505, 3379, 3380, 3452, 3487, 3488 |
| BmiI | GGNNCC | 8 cut(s) | 23, 1424, 1676, 1908, 2506, 3381, 3447, 3489 |
| BmsI | GCATC | 7 cut(s) | 433, 1162, 1248, 1581, 2440, 2722, 3280 |
| BpiI | GAAGAC | 1 cut(s) | 2964 |
| BpmI | CTGGAG | 3 cut(s) | 2730, 3420, 3594 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1355, 1752, 1844 |
| Bpu14I | TTCGAA | 1 cut(s) | 2995 |
| BpuEI | CTTGAG | 3 cut(s) | 1448, 1567, 2392 |
| BsaAI | YACGTR | 1 cut(s) | 412 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1440 |
| BsaI | GGTCTC | 1 cut(s) | 1976 |
| BsaJI | CCNNGG | 9 cut(s) | 1394, 1914, 1959, 2243, 2854, 3188, 3417, 3437, 3571 |
| BsaWI | WCCGGW | 2 cut(s) | 24, 1614 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 796, 826, 1152, 1182 |
| Bse118I | RCCGGY | 2 cut(s) | 24, 2681 |
| Bse1I | ACTGG | 7 cut(s) | 1687, 1810, 2580, 2625, 2866, 3199, 3403 |
| Bse3DI | GCAATG | 4 cut(s) | 234, 2560, 3084, 3300 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1440 |
| BseDI | CCNNGG | 9 cut(s) | 1394, 1914, 1959, 2243, 2854, 3188, 3417, 3437, 3571 |
| BseJI | GATNNNNATC | 1 cut(s) | 1440 |
| BseMI | GCAATG | 4 cut(s) | 234, 2560, 3084, 3300 |
| BseNI | ACTGG | 7 cut(s) | 1687, 1810, 2580, 2625, 2866, 3199, 3403 |
| BseRI | GAGGAG | 9 cut(s) | 289, 360, 416, 685, 874, 920, 923, 973, 976 |
| BseSI | GKGCMC | 2 cut(s) | 3383, 3491 |
| BseX3I | CGGCCG | 1 cut(s) | 2683 |
| BseYI | CCCAGC | 1 cut(s) | 1877 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2686 |
| BshFI | GGCC | 6 cut(s) | 1888, 2507, 2685, 2694, 3381, 3489 |
| BshNI | GGYRCC | 3 cut(s) | 21, 1674, 1906 |
| BshTI | ACCGGT | 1 cut(s) | 24 |
| BsiEI | CGRYCG | 1 cut(s) | 2686 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3228 |
| BsiSI | CCGG | 4 cut(s) | 25, 1615, 2682, 2695 |
| BslFI | GGGAC | 6 cut(s) | 527, 1436, 2624, 2853, 2872, 3205 |
| BsmAI | GTCTC | 4 cut(s) | 1776, 1976, 2495, 2725 |
| BsmFI | GGGAC | 6 cut(s) | 527, 1436, 2624, 2853, 2872, 3205 |
| BsmI | GAATGC | 2 cut(s) | 1285, 3349 |
| BsnI | GGCC | 6 cut(s) | 1888, 2507, 2685, 2694, 3381, 3489 |
| Bso31I | GGTCTC | 1 cut(s) | 1976 |
| BsoBI | CYCGRG | 1 cut(s) | 3228 |
| Bsp119I | TTCGAA | 1 cut(s) | 2995 |
| Bsp120I | GGGCCC | 2 cut(s) | 3379, 3487 |
| Bsp1286I | GDGCHC | 3 cut(s) | 851, 3383, 3491 |
| Bsp1407I | TGTACA | 1 cut(s) | 2428 |
| Bsp19I | CCATGG | 1 cut(s) | 2854 |
| BspACI | CCGC | 9 cut(s) | 190, 295, 562, 757, 955, 2032, 2304, 2918, 3245 |
| BspANI | GGCC | 6 cut(s) | 1888, 2507, 2685, 2694, 3381, 3489 |
| BspLI | GGNNCC | 8 cut(s) | 23, 1424, 1676, 1908, 2506, 3381, 3447, 3489 |
| BspMAI | CTGCAG | 2 cut(s) | 1210, 3238 |
| BspMI | ACCTGC | 2 cut(s) | 1352, 2239 |
| BspPI | GGATC | 6 cut(s) | 116, 306, 1220, 1436, 1816, 3274 |
| BspQI | GCTCTTC | 2 cut(s) | 146, 3141 |
| BspT104I | TTCGAA | 1 cut(s) | 2995 |
| BspT107I | GGYRCC | 3 cut(s) | 21, 1674, 1906 |
| BspTI | CTTAAG | 2 cut(s) | 737, 2471 |
| BspTNI | GGTCTC | 1 cut(s) | 1976 |
| BsrBI | CCGCTC | 3 cut(s) | 295, 562, 2918 |
| BsrDI | GCAATG | 4 cut(s) | 234, 2560, 3084, 3300 |
| BsrFI | RCCGGY | 2 cut(s) | 24, 2681 |
| BsrGI | TGTACA | 1 cut(s) | 2428 |
| BsrI | ACTGG | 7 cut(s) | 1687, 1810, 2580, 2625, 2866, 3199, 3403 |
| BssAI | RCCGGY | 2 cut(s) | 24, 2681 |
| BssECI | CCNNGG | 9 cut(s) | 1394, 1914, 1959, 2243, 2854, 3188, 3417, 3437, 3571 |
| BssNAI | GTATAC | 1 cut(s) | 1990 |
| BssT1I | CCWWGG | 2 cut(s) | 1959, 2854 |
| Bst1107I | GTATAC | 1 cut(s) | 1990 |
| Bst4CI | ACNGT | 6 cut(s) | 1288, 1940, 2221, 2669, 3119, 3501 |
| Bst6I | CTCTTC | 8 cut(s) | 146, 228, 548, 615, 632, 761, 3141, 3537 |
| BstAFI | CTTAAG | 2 cut(s) | 737, 2471 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 3242 |
| BstAUI | TGTACA | 1 cut(s) | 2428 |
| BstBAI | YACGTR | 1 cut(s) | 412 |
| BstBI | TTCGAA | 1 cut(s) | 2995 |
| BstC8I | GCNNGC | 6 cut(s) | 76, 1099, 1673, 2692, 3024, 3467 |
| BstDSI | CCRYGG | 1 cut(s) | 2854 |
| BstH2I | RGCGCY | 2 cut(s) | 143, 159 |
| BstHHI | GCGC | 2 cut(s) | 142, 158 |
| BstMAI | GTCTC | 4 cut(s) | 1776, 1976, 2495, 2725 |
| BstMCI | CGRYCG | 1 cut(s) | 2686 |
| BstSFI | CTRYAG | 3 cut(s) | 1206, 3072, 3234 |
| BstSLI | GKGCMC | 2 cut(s) | 3383, 3491 |
| BstV2I | GAAGAC | 1 cut(s) | 2964 |
| BstX2I | RGATCY | 6 cut(s) | 121, 1578, 2464, 2749, 3279, 3546 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1966, 2861 |
| BstYI | RGATCY | 6 cut(s) | 121, 1578, 2464, 2749, 3279, 3546 |
| BstZ17I | GTATAC | 1 cut(s) | 1990 |
| BstZI | CGGCCG | 1 cut(s) | 2683 |
| BsuRI | GGCC | 6 cut(s) | 1888, 2507, 2685, 2694, 3381, 3489 |
| BtgI | CCRYGG | 1 cut(s) | 2854 |
| BtsI | GCAGTG | 2 cut(s) | 1757, 3220 |
| BtsIMutI | CAGTG | 4 cut(s) | 1757, 1945, 2587, 3220 |
| BveI | ACCTGC | 2 cut(s) | 1352, 2239 |
| Cac8I | GCNNGC | 6 cut(s) | 76, 1099, 1673, 2692, 3024, 3467 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1205, 1759 |
| CfoI | GCGC | 2 cut(s) | 142, 158 |
| Cfr10I | RCCGGY | 2 cut(s) | 24, 2681 |
| Cfr13I | GGNCC | 9 cut(s) | 1423, 1858, 1886, 2505, 3379, 3380, 3452, 3487, 3488 |
| Csp6I | GTAC | 6 cut(s) | 409, 781, 1832, 2055, 2429, 2679 |
| CspAI | ACCGGT | 1 cut(s) | 24 |
| CviAII | CATG | 9 cut(s) | 1640, 2134, 2161, 2332, 2360, 2477, 2501, 2855, 3253 |
| CviQI | GTAC | 6 cut(s) | 409, 781, 1832, 2055, 2429, 2679 |
| DraI | TTTAAA | 1 cut(s) | 2884 |
| DriI | GACNNNNNGTC | 2 cut(s) | 1048, 3128 |
| EaeI | YGGCCR | 2 cut(s) | 2683, 2692 |
| EagI | CGGCCG | 1 cut(s) | 2683 |
| Eam1104I | CTCTTC | 8 cut(s) | 146, 228, 548, 615, 632, 761, 3141, 3537 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 1048, 3128 |
| EarI | CTCTTC | 8 cut(s) | 146, 228, 548, 615, 632, 761, 3141, 3537 |
| EciI | GGCGGA | 1 cut(s) | 2319 |
| EclXI | CGGCCG | 1 cut(s) | 2683 |
| Eco130I | CCWWGG | 2 cut(s) | 1959, 2854 |
| Eco24I | GRGCYC | 3 cut(s) | 851, 3383, 3491 |
| Eco31I | GGTCTC | 1 cut(s) | 1976 |
| Eco32I | GATATC | 5 cut(s) | 64, 1945, 2483, 3013, 3596 |
| Eco47I | GGWCC | 3 cut(s) | 1423, 1858, 3452 |
| Eco52I | CGGCCG | 1 cut(s) | 2683 |
| Eco57I | CTGAAG | 2 cut(s) | 170, 2048 |
| Eco88I | CYCGRG | 1 cut(s) | 3228 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1423, 3380, 3487, 3488 |
| EcoRI | GAATTC | 1 cut(s) | 110 |
| EcoRV | GATATC | 5 cut(s) | 64, 1945, 2483, 3013, 3596 |
| EcoT14I | CCWWGG | 2 cut(s) | 1959, 2854 |
| EcoT38I | GRGCYC | 3 cut(s) | 851, 3383, 3491 |
| ErhI | CCWWGG | 2 cut(s) | 1959, 2854 |
| FaeI | CATG | 9 cut(s) | 1643, 2137, 2164, 2335, 2363, 2480, 2504, 2858, 3256 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 99, 131, 3458, 3490 |
| FaqI | GGGAC | 6 cut(s) | 527, 1436, 2624, 2853, 2872, 3205 |
| FatI | CATG | 9 cut(s) | 1639, 2133, 2160, 2331, 2359, 2476, 2500, 2854, 3252 |
| FauI | CCCGC | 2 cut(s) | 288, 2025 |
| FblI | GTMKAC | 1 cut(s) | 1989 |
| FriOI | GRGCYC | 3 cut(s) | 851, 3383, 3491 |
| FspBI | CTAG | 6 cut(s) | 209, 252, 2592, 2600, 2736, 3348 |
| GlaI | GCGC | 2 cut(s) | 141, 157 |
| GsaI | CCCAGC | 1 cut(s) | 1881 |
| GsuI | CTGGAG | 3 cut(s) | 2730, 3420, 3594 |
| HaeII | RGCGCY | 2 cut(s) | 143, 159 |
| HaeIII | GGCC | 6 cut(s) | 1888, 2507, 2685, 2694, 3381, 3489 |
| HapII | CCGG | 4 cut(s) | 25, 1615, 2682, 2695 |
| HhaI | GCGC | 2 cut(s) | 142, 158 |
| Hin1II | CATG | 9 cut(s) | 1643, 2137, 2164, 2335, 2363, 2480, 2504, 2858, 3256 |
| Hin6I | GCGC | 2 cut(s) | 140, 156 |
| HinP1I | GCGC | 2 cut(s) | 140, 156 |
| HincII | GTYRAC | 1 cut(s) | 1519 |
| HindII | GTYRAC | 1 cut(s) | 1519 |
| HindIII | AAGCTT | 1 cut(s) | 1236 |
| HpaII | CCGG | 4 cut(s) | 25, 1615, 2682, 2695 |
| HphI | GGTGA | 7 cut(s) | 1267, 1358, 1582, 2635, 2657, 3020, 3352 |
| Hpy166II | GTNNAC | 7 cut(s) | 1519, 1990, 2024, 2532, 2665, 3122, 3396 |
| Hpy8I | GTNNAC | 7 cut(s) | 1519, 1990, 2024, 2532, 2665, 3122, 3396 |
| Hpy99I | CGWCG | 3 cut(s) | 14, 107, 132 |
| HpyCH4III | ACNGT | 6 cut(s) | 1288, 1940, 2221, 2669, 3119, 3501 |
| HpyCH4IV | ACGT | 5 cut(s) | 411, 604, 1122, 2677, 3374 |
| HpySE526I | ACGT | 5 cut(s) | 411, 604, 1122, 2677, 3374 |
| Hsp92II | CATG | 9 cut(s) | 1643, 2137, 2164, 2335, 2363, 2480, 2504, 2858, 3256 |
| HspAI | GCGC | 2 cut(s) | 140, 156 |
| LguI | GCTCTTC | 2 cut(s) | 146, 3141 |
| LmnI | GCTCC | 7 cut(s) | 292, 559, 742, 1918, 2611, 2749, 3401 |
| LweI | GCATC | 7 cut(s) | 433, 1162, 1248, 1581, 2440, 2722, 3280 |
| MaeI | CTAG | 6 cut(s) | 209, 252, 2592, 2600, 2736, 3348 |
| MaeII | ACGT | 5 cut(s) | 411, 604, 1122, 2677, 3374 |
| MaeIII | GTNAC | 4 cut(s) | 359, 2119, 2385, 3358 |
| MbiI | CCGCTC | 3 cut(s) | 295, 562, 2918 |
| MfeI | CAATTG | 3 cut(s) | 1299, 1622, 2336 |
| MflI | RGATCY | 6 cut(s) | 121, 1578, 2464, 2749, 3279, 3546 |
| MhlI | GDGCHC | 3 cut(s) | 851, 3383, 3491 |
| MlyI | GAGTC | 8 cut(s) | 286, 364, 546, 1060, 1118, 1403, 2537, 3079 |
| MmeI | TCCRAC | 3 cut(s) | 1777, 2653, 3300 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2991 |
| MseI | TTAA | 8 cut(s) | 738, 986, 1191, 2472, 2547, 2883, 2889, 3527 |
| MslI | CAYNNNNRTG | 4 cut(s) | 705, 831, 1769, 3288 |
| MspA1I | CMGCKG | 1 cut(s) | 1202 |
| MspCI | CTTAAG | 2 cut(s) | 737, 2471 |
| MspI | CCGG | 4 cut(s) | 25, 1615, 2682, 2695 |
| MunI | CAATTG | 3 cut(s) | 1299, 1622, 2336 |
| Mva1269I | GAATGC | 2 cut(s) | 1285, 3349 |
| NcoI | CCATGG | 1 cut(s) | 2854 |
| NlaIII | CATG | 9 cut(s) | 1643, 2137, 2164, 2335, 2363, 2480, 2504, 2858, 3256 |
| NlaIV | GGNNCC | 8 cut(s) | 23, 1424, 1676, 1908, 2506, 3381, 3447, 3489 |
| NmeAIII | GCCGAG | 1 cut(s) | 2224 |
| NmuCI | GTSAC | 2 cut(s) | 359, 3358 |
| NspV | TTCGAA | 1 cut(s) | 2995 |
| PciSI | GCTCTTC | 2 cut(s) | 146, 3141 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 364, 2683 |
| PctI | GAATGC | 2 cut(s) | 1285, 3349 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2991 |
| PfeI | GAWTC | 8 cut(s) | 167, 395, 722, 875, 1079, 1403, 1933, 2969 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 3146 |
| PfoI | TCCNGGA | 1 cut(s) | 3448 |
| PinAI | ACCGGT | 1 cut(s) | 24 |
| PleI | GAGTC | 8 cut(s) | 285, 363, 545, 1060, 1117, 1403, 2536, 3079 |
| PpsI | GAGTC | 8 cut(s) | 285, 363, 545, 1060, 1117, 1403, 2536, 3079 |
| Ppu21I | YACGTR | 1 cut(s) | 412 |
| PpuMI | RGGWCCY | 1 cut(s) | 1423 |
| Psp1406I | AACGTT | 1 cut(s) | 3374 |
| Psp5II | RGGWCCY | 1 cut(s) | 1423 |
| PspFI | CCCAGC | 1 cut(s) | 1877 |
| PspN4I | GGNNCC | 8 cut(s) | 23, 1424, 1676, 1908, 2506, 3381, 3447, 3489 |
| PspOMI | GGGCCC | 2 cut(s) | 3379, 3487 |
| PspPI | GGNCC | 9 cut(s) | 1423, 1858, 1886, 2505, 3379, 3380, 3452, 3487, 3488 |
| PspPPI | RGGWCCY | 1 cut(s) | 1423 |
| PstI | CTGCAG | 2 cut(s) | 1210, 3238 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1205, 1759 |
| PsuI | RGATCY | 6 cut(s) | 121, 1578, 2464, 2749, 3279, 3546 |
| PvuII | CAGCTG | 1 cut(s) | 1202 |
| RsaI | GTAC | 6 cut(s) | 410, 782, 1833, 2056, 2430, 2680 |
| RsaNI | GTAC | 6 cut(s) | 409, 781, 1832, 2055, 2429, 2679 |
| RseI | CAYNNNNRTG | 4 cut(s) | 705, 831, 1769, 3288 |
| SapI | GCTCTTC | 2 cut(s) | 146, 3141 |
| SaqAI | TTAA | 8 cut(s) | 738, 986, 1191, 2472, 2547, 2883, 2889, 3527 |
| Sau96I | GGNCC | 9 cut(s) | 1423, 1858, 1886, 2505, 3379, 3380, 3452, 3487, 3488 |
| SchI | GAGTC | 8 cut(s) | 286, 364, 546, 1060, 1118, 1403, 2537, 3079 |
| SduI | GDGCHC | 3 cut(s) | 851, 3383, 3491 |
| SfaNI | GCATC | 7 cut(s) | 433, 1162, 1248, 1581, 2440, 2722, 3280 |
| SfcI | CTRYAG | 3 cut(s) | 1206, 3072, 3234 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2691 |
| SfuI | TTCGAA | 1 cut(s) | 2995 |
| SinI | GGWCC | 3 cut(s) | 1423, 1858, 3452 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 705, 831, 1769, 3288 |
| SmlI | CTYRAG | 5 cut(s) | 737, 1427, 1582, 2371, 2471 |
| SmoI | CTYRAG | 5 cut(s) | 737, 1427, 1582, 2371, 2471 |
| SsiI | CCGC | 9 cut(s) | 190, 295, 562, 757, 955, 2032, 2304, 2918, 3245 |
| SspI | AATATT | 1 cut(s) | 2200 |
| SspMI | CTAG | 6 cut(s) | 209, 252, 2592, 2600, 2736, 3348 |
| StyI | CCWWGG | 2 cut(s) | 1959, 2854 |
| TaaI | ACNGT | 6 cut(s) | 1288, 1940, 2221, 2669, 3119, 3501 |
| TaiI | ACGT | 5 cut(s) | 414, 607, 1125, 2680, 3377 |
| TatI | WGTACW | 4 cut(s) | 780, 1831, 2054, 2428 |
| TauI | GCSGC | 3 cut(s) | 760, 2921, 3248 |
| TfiI | GAWTC | 8 cut(s) | 167, 395, 722, 875, 1079, 1403, 1933, 2969 |
| Tru1I | TTAA | 8 cut(s) | 738, 986, 1191, 2472, 2547, 2883, 2889, 3527 |
| Tru9I | TTAA | 8 cut(s) | 738, 986, 1191, 2472, 2547, 2883, 2889, 3527 |
| TscAI | CASTG | 4 cut(s) | 1764, 1945, 2587, 3220 |
| TseFI | GTSAC | 2 cut(s) | 359, 3358 |
| Tsp45I | GTSAC | 2 cut(s) | 359, 3358 |
| TspGWI | ACGGA | 2 cut(s) | 1538, 2007 |
| TspRI | CASTG | 4 cut(s) | 1764, 1945, 2587, 3220 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 3146 |
| Vha464I | CTTAAG | 2 cut(s) | 737, 2471 |
| VpaK11BI | GGWCC | 3 cut(s) | 1423, 1858, 3452 |
| XapI | RAATTY | 5 cut(s) | 110, 1555, 2415, 2629, 2885 |
| XmiI | GTMKAC | 1 cut(s) | 1989 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2991 |
| XspI | CTAG | 6 cut(s) | 209, 252, 2592, 2600, 2736, 3348 |