DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1703, 1748 |
| AarI | CACCTGC | 1 cut(s) | 1057 |
| Acc36I | ACCTGC | 3 cut(s) | 651, 950, 1057 |
| AccB1I | GGYRCC | 2 cut(s) | 1476, 1708 |
| AccBSI | CCGCTC | 1 cut(s) | 423 |
| AccII | CGCG | 2 cut(s) | 89, 299 |
| AciI | CCGC | 7 cut(s) | 204, 297, 400, 423, 538, 814, 1834 |
| AclI | AACGTT | 1 cut(s) | 2516 |
| AclWI | GGATC | 5 cut(s) | 1067, 1238, 1329, 1668, 2274 |
| AcoI | YGGCCR | 2 cut(s) | 1125, 2006 |
| AcsI | RAATTY | 4 cut(s) | 28, 1093, 1357, 2217 |
| AcvI | CACGTG | 1 cut(s) | 883 |
| AfaI | GTAC | 3 cut(s) | 1858, 1888, 2482 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1705, 1793, 1866, 2050 |
| AflIII | ACRYGT | 1 cut(s) | 2246 |
| AgsI | TTSAA | 7 cut(s) | 33, 1170, 1435, 1831, 1973, 2057, 2147 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1770 |
| AhlI | ACTAGT | 1 cut(s) | 2381 |
| AjnI | CCWGG | 4 cut(s) | 920, 1195, 1472, 2049 |
| Alw26I | GTCTC | 7 cut(s) | 238, 297, 348, 1387, 1578, 1778, 2297 |
| AlwI | GGATC | 5 cut(s) | 1067, 1238, 1329, 1668, 2274 |
| AoxI | GGCC | 5 cut(s) | 1125, 1688, 1696, 2006, 2485 |
| ApeKI | GCWGC | 5 cut(s) | 1043, 1105, 1133, 1636, 1841 |
| ApoI | RAATTY | 4 cut(s) | 28, 1093, 1357, 2217 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1952, 1984 |
| AseI | ATTAAT | 1 cut(s) | 36 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1358 |
| AspA2I | CCTAGG | 1 cut(s) | 173 |
| AspLEI | GCGC | 1 cut(s) | 2548 |
| AspS9I | GGNCC | 2 cut(s) | 1688, 2154 |
| AsuC2I | CCSGG | 1 cut(s) | 1863 |
| AsuHPI | GGTGA | 7 cut(s) | 293, 1169, 1297, 1384, 2039, 2437, 2459 |
| AvaII | GGWCC | 1 cut(s) | 2154 |
| AvrII | CCTAGG | 1 cut(s) | 173 |
| BalI | TGGCCA | 1 cut(s) | 1127 |
| BanI | GGYRCC | 2 cut(s) | 1476, 1708 |
| BanII | GRGCYC | 1 cut(s) | 698 |
| BauI | CACGAG | 1 cut(s) | 349 |
| BbrPI | CACGTG | 1 cut(s) | 883 |
| BbsI | GAAGAC | 2 cut(s) | 1260, 1266 |
| BbvCI | CCTCAGC | 2 cut(s) | 867, 1646 |
| BbvI | GCAGC | 5 cut(s) | 1030, 1092, 1120, 1623, 1828 |
| BccI | CCATC | 7 cut(s) | 209, 272, 448, 674, 998, 1232, 1679 |
| BceAI | ACGGC | 2 cut(s) | 620, 995 |
| BciT130I | CCWGG | 4 cut(s) | 922, 1197, 1474, 2051 |
| BclI | TGATCA | 1 cut(s) | 2427 |
| BcnI | CCSGG | 1 cut(s) | 1863 |
| BcoDI | GTCTC | 7 cut(s) | 238, 297, 348, 1387, 1578, 1778, 2297 |
| BcuI | ACTAGT | 1 cut(s) | 2381 |
| BfaI | CTAG | 8 cut(s) | 174, 288, 339, 441, 498, 1316, 2382, 2394 |
| BfmI | CTRYAG | 3 cut(s) | 1098, 1134, 1913 |
| BfuAI | ACCTGC | 3 cut(s) | 651, 950, 1057 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2493 |
| BglII | AGATCT | 1 cut(s) | 2551 |
| BisI | GCNGC | 6 cut(s) | 401, 1044, 1106, 1134, 1637, 1842 |
| BlnI | CCTAGG | 1 cut(s) | 173 |
| BlsI | GCNGC | 6 cut(s) | 402, 1045, 1107, 1135, 1638, 1843 |
| Bme1390I | CCNGG | 5 cut(s) | 922, 1197, 1474, 1863, 2051 |
| Bme18I | GGWCC | 1 cut(s) | 2154 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1770 |
| BmgT120I | GGNCC | 2 cut(s) | 1688, 2154 |
| BmiI | GGNNCC | 3 cut(s) | 1009, 1478, 1710 |
| BmrFI | CCNGG | 5 cut(s) | 922, 1197, 1474, 1863, 2051 |
| BmrI | ACTGGG | 1 cut(s) | 1778 |
| BmsI | GCATC | 3 cut(s) | 412, 1072, 2524 |
| BmuI | ACTGGG | 1 cut(s) | 1778 |
| BpiI | GAAGAC | 2 cut(s) | 1260, 1266 |
| BpmI | CTGGAG | 2 cut(s) | 1701, 2427 |
| Bpu10I | CCTNAGC | 6 cut(s) | 867, 1554, 1612, 1646, 2261, 2273 |
| BpuEI | CTTGAG | 2 cut(s) | 950, 1095 |
| BpuMI | CCSGG | 1 cut(s) | 1863 |
| Bsa29I | ATCGAT | 2 cut(s) | 22, 2596 |
| BsaAI | YACGTR | 2 cut(s) | 883, 2069 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1242 |
| BsaI | GGTCTC | 2 cut(s) | 1387, 1778 |
| BsaJI | CCNNGG | 3 cut(s) | 173, 1196, 1861 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1705, 1793, 1866, 2050 |
| Bse1I | ACTGG | 5 cut(s) | 144, 1145, 1489, 1612, 1773 |
| Bse3DI | GCAATG | 2 cut(s) | 153, 1596 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1242 |
| BseBI | CCWGG | 4 cut(s) | 922, 1197, 1474, 2051 |
| BseCI | ATCGAT | 2 cut(s) | 22, 2596 |
| BseDI | CCNNGG | 3 cut(s) | 173, 1196, 1861 |
| BseGI | GGATG | 9 cut(s) | 586, 685, 784, 898, 1009, 1066, 1456, 1875, 1945 |
| BseJI | GATNNNNATC | 1 cut(s) | 1242 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1705, 1793, 1866, 2050 |
| BseMI | GCAATG | 2 cut(s) | 153, 1596 |
| BseNI | ACTGG | 5 cut(s) | 144, 1145, 1489, 1612, 1773 |
| BseXI | GCAGC | 5 cut(s) | 1030, 1092, 1120, 1623, 1828 |
| BsgI | GTGCAG | 1 cut(s) | 1029 |
| Bsh1236I | CGCG | 2 cut(s) | 89, 299 |
| Bsh1285I | CGRYCG | 1 cut(s) | 22 |
| BshFI | GGCC | 5 cut(s) | 1127, 1690, 1698, 2008, 2487 |
| BshNI | GGYRCC | 2 cut(s) | 1476, 1708 |
| BshVI | ATCGAT | 2 cut(s) | 22, 2596 |
| BsiEI | CGRYCG | 1 cut(s) | 22 |
| BsiSI | CCGG | 1 cut(s) | 1863 |
| BslFI | GGGAC | 3 cut(s) | 527, 1238, 1439 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1705, 1793, 1866, 2050 |
| BsmAI | GTCTC | 7 cut(s) | 238, 297, 348, 1387, 1578, 1778, 2297 |
| BsmFI | GGGAC | 3 cut(s) | 527, 1238, 1439 |
| BsnI | GGCC | 5 cut(s) | 1127, 1690, 1698, 2008, 2487 |
| Bso31I | GGTCTC | 2 cut(s) | 1387, 1778 |
| Bsp1286I | GDGCHC | 1 cut(s) | 698 |
| Bsp1407I | TGTACA | 1 cut(s) | 1886 |
| Bsp68I | TCGCGA | 1 cut(s) | 89 |
| BspACI | CCGC | 7 cut(s) | 204, 297, 400, 423, 538, 814, 1834 |
| BspANI | GGCC | 5 cut(s) | 1127, 1690, 1698, 2008, 2487 |
| BspDI | ATCGAT | 2 cut(s) | 22, 2596 |
| BspFNI | CGCG | 2 cut(s) | 89, 299 |
| BspLI | GGNNCC | 3 cut(s) | 1009, 1478, 1710 |
| BspMAI | CTGCAG | 1 cut(s) | 1138 |
| BspMI | ACCTGC | 3 cut(s) | 651, 950, 1057 |
| BspPI | GGATC | 5 cut(s) | 1067, 1238, 1329, 1668, 2274 |
| BspT107I | GGYRCC | 2 cut(s) | 1476, 1708 |
| BspTNI | GGTCTC | 2 cut(s) | 1387, 1778 |
| BsrBI | CCGCTC | 1 cut(s) | 423 |
| BsrDI | GCAATG | 2 cut(s) | 153, 1596 |
| BsrGI | TGTACA | 1 cut(s) | 1886 |
| BsrI | ACTGG | 5 cut(s) | 144, 1145, 1489, 1612, 1773 |
| BssECI | CCNNGG | 3 cut(s) | 173, 1196, 1861 |
| BssSI | CACGAG | 1 cut(s) | 349 |
| BssT1I | CCWWGG | 1 cut(s) | 173 |
| Bst2BI | CACGAG | 1 cut(s) | 349 |
| Bst2UI | CCWGG | 4 cut(s) | 922, 1197, 1474, 2051 |
| Bst4CI | ACNGT | 4 cut(s) | 1102, 1742, 2257, 2471 |
| Bst6I | CTCTTC | 2 cut(s) | 701, 948 |
| BstAUI | TGTACA | 1 cut(s) | 1886 |
| BstBAI | YACGTR | 2 cut(s) | 883, 2069 |
| BstC8I | GCNNGC | 4 cut(s) | 91, 1125, 2494, 2583 |
| BstF5I | GGATG | 9 cut(s) | 586, 685, 784, 898, 1009, 1066, 1456, 1875, 1945 |
| BstFNI | CGCG | 2 cut(s) | 89, 299 |
| BstHHI | GCGC | 1 cut(s) | 2548 |
| BstMAI | GTCTC | 7 cut(s) | 238, 297, 348, 1387, 1578, 1778, 2297 |
| BstMCI | CGRYCG | 1 cut(s) | 22 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 1049, 1133, 1642, 1729, 2493, 2543 |
| BstNI | CCWGG | 4 cut(s) | 922, 1197, 1474, 2051 |
| BstNSI | RCATGY | 1 cut(s) | 2243 |
| BstSCI | CCNGG | 5 cut(s) | 920, 1195, 1472, 1861, 2049 |
| BstSFI | CTRYAG | 3 cut(s) | 1098, 1134, 1913 |
| BstUI | CGCG | 2 cut(s) | 89, 299 |
| BstV1I | GCAGC | 5 cut(s) | 1030, 1092, 1120, 1623, 1828 |
| BstV2I | GAAGAC | 2 cut(s) | 1260, 1266 |
| BstX2I | RGATCY | 2 cut(s) | 2266, 2551 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1768, 1820 |
| BstYI | RGATCY | 2 cut(s) | 2266, 2551 |
| Bsu15I | ATCGAT | 2 cut(s) | 22, 2596 |
| BsuRI | GGCC | 5 cut(s) | 1127, 1690, 1698, 2008, 2487 |
| BsuTUI | ATCGAT | 2 cut(s) | 22, 2596 |
| BtgZI | GCGATG | 1 cut(s) | 2370 |
| BtsCI | GGATG | 9 cut(s) | 586, 685, 784, 898, 1009, 1066, 1456, 1875, 1945 |
| BtsI | GCAGTG | 1 cut(s) | 1566 |
| BtsIMutI | CAGTG | 6 cut(s) | 990, 1107, 1566, 1747, 1766, 2253 |
| BtuMI | TCGCGA | 1 cut(s) | 89 |
| BveI | ACCTGC | 3 cut(s) | 651, 950, 1057 |
| Cac8I | GCNNGC | 4 cut(s) | 91, 1125, 2494, 2583 |
| CfoI | GCGC | 1 cut(s) | 2548 |
| Cfr13I | GGNCC | 2 cut(s) | 1688, 2154 |
| ClaI | ATCGAT | 2 cut(s) | 22, 2596 |
| CsiI | ACCWGGT | 1 cut(s) | 2049 |
| Csp6I | GTAC | 3 cut(s) | 1857, 1887, 2481 |
| CviAII | CATG | 5 cut(s) | 1015, 1442, 2240, 2279, 2303 |
| CviQI | GTAC | 3 cut(s) | 1857, 1887, 2481 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1770 |
| EaeI | YGGCCR | 2 cut(s) | 1125, 2006 |
| Eam1104I | CTCTTC | 2 cut(s) | 701, 948 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1770 |
| EarI | CTCTTC | 2 cut(s) | 701, 948 |
| Eco130I | CCWWGG | 1 cut(s) | 173 |
| Eco24I | GRGCYC | 1 cut(s) | 698 |
| Eco31I | GGTCTC | 2 cut(s) | 1387, 1778 |
| Eco32I | GATATC | 1 cut(s) | 2285 |
| Eco47I | GGWCC | 1 cut(s) | 2154 |
| Eco72I | CACGTG | 1 cut(s) | 883 |
| EcoRI | GAATTC | 1 cut(s) | 1093 |
| EcoRII | CCWGG | 4 cut(s) | 920, 1195, 1472, 2049 |
| EcoRV | GATATC | 1 cut(s) | 2285 |
| EcoT14I | CCWWGG | 1 cut(s) | 173 |
| EcoT38I | GRGCYC | 1 cut(s) | 698 |
| ErhI | CCWWGG | 1 cut(s) | 173 |
| FaeI | CATG | 5 cut(s) | 1018, 1445, 2243, 2282, 2306 |
| FaqI | GGGAC | 3 cut(s) | 527, 1238, 1439 |
| FatI | CATG | 5 cut(s) | 1014, 1441, 2239, 2278, 2302 |
| FauI | CCCGC | 1 cut(s) | 1827 |
| FauNDI | CATATG | 1 cut(s) | 1630 |
| FbaI | TGATCA | 1 cut(s) | 2427 |
| Fnu4HI | GCNGC | 6 cut(s) | 401, 1044, 1106, 1134, 1637, 1842 |
| FokI | GGATG | 9 cut(s) | 593, 692, 791, 905, 1016, 1073, 1463, 1882, 1952 |
| FriOI | GRGCYC | 1 cut(s) | 698 |
| Fsp4HI | GCNGC | 6 cut(s) | 401, 1044, 1106, 1134, 1637, 1842 |
| FspBI | CTAG | 8 cut(s) | 174, 288, 339, 441, 498, 1316, 2382, 2394 |
| GlaI | GCGC | 1 cut(s) | 2547 |
| GluI | GCNGC | 6 cut(s) | 401, 1044, 1106, 1134, 1637, 1842 |
| GsuI | CTGGAG | 2 cut(s) | 1701, 2427 |
| HaeIII | GGCC | 5 cut(s) | 1127, 1690, 1698, 2008, 2487 |
| HapII | CCGG | 1 cut(s) | 1863 |
| HhaI | GCGC | 1 cut(s) | 2548 |
| Hin1II | CATG | 5 cut(s) | 1018, 1445, 2243, 2282, 2306 |
| Hin6I | GCGC | 1 cut(s) | 2546 |
| HinP1I | GCGC | 1 cut(s) | 2546 |
| HincII | GTYRAC | 1 cut(s) | 973 |
| HindII | GTYRAC | 1 cut(s) | 973 |
| HpaII | CCGG | 1 cut(s) | 1863 |
| HphI | GGTGA | 7 cut(s) | 293, 1169, 1297, 1384, 2039, 2437, 2459 |
| Hpy166II | GTNNAC | 5 cut(s) | 973, 1275, 1580, 2122, 2467 |
| Hpy188III | TCNNGA | 8 cut(s) | 88, 212, 830, 1351, 2327, 2394, 2444, 2591 |
| Hpy8I | GTNNAC | 5 cut(s) | 973, 1275, 1580, 2122, 2467 |
| HpyAV | CCTTC | 5 cut(s) | 172, 499, 525, 881, 1369 |
| HpyCH4III | ACNGT | 4 cut(s) | 1102, 1742, 2257, 2471 |
| HpyCH4IV | ACGT | 6 cut(s) | 882, 969, 2039, 2068, 2246, 2516 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 1049, 1133, 1642, 1729, 2493, 2543 |
| HpySE526I | ACGT | 6 cut(s) | 882, 969, 2039, 2068, 2246, 2516 |
| Hsp92II | CATG | 5 cut(s) | 1018, 1445, 2243, 2282, 2306 |
| HspAI | GCGC | 1 cut(s) | 2546 |
| Ksp22I | TGATCA | 1 cut(s) | 2427 |
| LmnI | GCTCC | 3 cut(s) | 181, 1007, 1720 |
| Lsp1109I | GCAGC | 5 cut(s) | 1030, 1092, 1120, 1623, 1828 |
| LweI | GCATC | 3 cut(s) | 412, 1072, 2524 |
| MabI | ACCWGGT | 1 cut(s) | 2049 |
| MaeI | CTAG | 8 cut(s) | 174, 288, 339, 441, 498, 1316, 2382, 2394 |
| MaeII | ACGT | 6 cut(s) | 882, 969, 2039, 2068, 2246, 2516 |
| MaeIII | GTNAC | 4 cut(s) | 653, 1285, 1921, 2249 |
| MbiI | CCGCTC | 1 cut(s) | 423 |
| MfeI | CAATTG | 2 cut(s) | 1424, 1845 |
| MflI | RGATCY | 2 cut(s) | 2266, 2551 |
| MhlI | GDGCHC | 1 cut(s) | 698 |
| MlsI | TGGCCA | 1 cut(s) | 1127 |
| MluNI | TGGCCA | 1 cut(s) | 1127 |
| MlyI | GAGTC | 1 cut(s) | 2014 |
| Mox20I | TGGCCA | 1 cut(s) | 1127 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1358 |
| MscI | TGGCCA | 1 cut(s) | 1127 |
| MseI | TTAA | 8 cut(s) | 36, 270, 845, 1467, 2078, 2349, 2409, 2602 |
| MslI | CAYNNNNRTG | 3 cut(s) | 213, 678, 1370 |
| Msp20I | TGGCCA | 1 cut(s) | 1127 |
| MspA1I | CMGCKG | 1 cut(s) | 1043 |
| MspI | CCGG | 1 cut(s) | 1863 |
| MspR9I | CCNGG | 5 cut(s) | 922, 1197, 1474, 1863, 2051 |
| MunI | CAATTG | 2 cut(s) | 1424, 1845 |
| MvaI | CCWGG | 4 cut(s) | 922, 1197, 1474, 2051 |
| MvnI | CGCG | 2 cut(s) | 89, 299 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 1049, 1133, 1642, 1729, 2493, 2543 |
| NciI | CCSGG | 1 cut(s) | 1863 |
| NdeI | CATATG | 1 cut(s) | 1630 |
| NlaIII | CATG | 5 cut(s) | 1018, 1445, 2243, 2282, 2306 |
| NlaIV | GGNNCC | 3 cut(s) | 1009, 1478, 1710 |
| NmeAIII | GCCGAG | 1 cut(s) | 2513 |
| NmuCI | GTSAC | 2 cut(s) | 1285, 2249 |
| NruI | TCGCGA | 1 cut(s) | 89 |
| NspI | RCATGY | 1 cut(s) | 2243 |
| PaqCI | CACCTGC | 1 cut(s) | 1057 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2485 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1358 |
| PkrI | GCNGC | 6 cut(s) | 402, 1045, 1107, 1135, 1638, 1843 |
| Ple19I | CGATCG | 1 cut(s) | 22 |
| PleI | GAGTC | 1 cut(s) | 2014 |
| PmaCI | CACGTG | 1 cut(s) | 883 |
| PmlI | CACGTG | 1 cut(s) | 883 |
| PpsI | GAGTC | 1 cut(s) | 2014 |
| Ppu21I | YACGTR | 2 cut(s) | 883, 2069 |
| PshBI | ATTAAT | 1 cut(s) | 36 |
| PsiI | TTATAA | 2 cut(s) | 1703, 1748 |
| Psp1406I | AACGTT | 1 cut(s) | 2516 |
| Psp6I | CCWGG | 4 cut(s) | 920, 1195, 1472, 2049 |
| PspCI | CACGTG | 1 cut(s) | 883 |
| PspGI | CCWGG | 4 cut(s) | 920, 1195, 1472, 2049 |
| PspN4I | GGNNCC | 3 cut(s) | 1009, 1478, 1710 |
| PspPI | GGNCC | 2 cut(s) | 1688, 2154 |
| PstI | CTGCAG | 1 cut(s) | 1138 |
| PsuI | RGATCY | 2 cut(s) | 2266, 2551 |
| PvuI | CGATCG | 1 cut(s) | 22 |
| PvuII | CAGCTG | 1 cut(s) | 1043 |
| RruI | TCGCGA | 1 cut(s) | 89 |
| RsaI | GTAC | 3 cut(s) | 1858, 1888, 2482 |
| RsaNI | GTAC | 3 cut(s) | 1857, 1887, 2481 |
| RseI | CAYNNNNRTG | 3 cut(s) | 213, 678, 1370 |
| SaqAI | TTAA | 8 cut(s) | 36, 270, 845, 1467, 2078, 2349, 2409, 2602 |
| SatI | GCNGC | 6 cut(s) | 401, 1044, 1106, 1134, 1637, 1842 |
| Sau96I | GGNCC | 2 cut(s) | 1688, 2154 |
| SchI | GAGTC | 1 cut(s) | 2014 |
| ScrFI | CCNGG | 5 cut(s) | 922, 1197, 1474, 1863, 2051 |
| SduI | GDGCHC | 1 cut(s) | 698 |
| SexAI | ACCWGGT | 1 cut(s) | 2049 |
| SfaNI | GCATC | 3 cut(s) | 412, 1072, 2524 |
| SfcI | CTRYAG | 3 cut(s) | 1098, 1134, 1913 |
| SinI | GGWCC | 1 cut(s) | 2154 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 213, 678, 1370 |
| SmlI | CTYRAG | 2 cut(s) | 929, 1110 |
| SmoI | CTYRAG | 2 cut(s) | 929, 1110 |
| SpeI | ACTAGT | 1 cut(s) | 2381 |
| SsiI | CCGC | 7 cut(s) | 204, 297, 400, 423, 538, 814, 1834 |
| SspI | AATATT | 1 cut(s) | 2002 |
| SspMI | CTAG | 8 cut(s) | 174, 288, 339, 441, 498, 1316, 2382, 2394 |
| StyD4I | CCNGG | 5 cut(s) | 920, 1195, 1472, 1861, 2049 |
| StyI | CCWWGG | 1 cut(s) | 173 |
| TaaI | ACNGT | 4 cut(s) | 1102, 1742, 2257, 2471 |
| TaiI | ACGT | 6 cut(s) | 885, 972, 2042, 2071, 2249, 2519 |
| TatI | WGTACW | 1 cut(s) | 1886 |
| TauI | GCSGC | 1 cut(s) | 403 |
| Tru1I | TTAA | 8 cut(s) | 36, 270, 845, 1467, 2078, 2349, 2409, 2602 |
| Tru9I | TTAA | 8 cut(s) | 36, 270, 845, 1467, 2078, 2349, 2409, 2602 |
| TscAI | CASTG | 6 cut(s) | 997, 1107, 1566, 1747, 1773, 2260 |
| TseFI | GTSAC | 2 cut(s) | 1285, 2249 |
| TseI | GCWGC | 5 cut(s) | 1043, 1105, 1133, 1636, 1841 |
| Tsp45I | GTSAC | 2 cut(s) | 1285, 2249 |
| TspGWI | ACGGA | 2 cut(s) | 990, 1908 |
| TspRI | CASTG | 6 cut(s) | 997, 1107, 1566, 1747, 1773, 2260 |
| VpaK11BI | GGWCC | 1 cut(s) | 2154 |
| VspI | ATTAAT | 1 cut(s) | 36 |
| XapI | RAATTY | 4 cut(s) | 28, 1093, 1357, 2217 |
| XbaI | TCTAGA | 1 cut(s) | 2393 |
| XceI | RCATGY | 1 cut(s) | 2243 |
| XmaJI | CCTAGG | 1 cut(s) | 173 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1358 |
| XspI | CTAG | 8 cut(s) | 174, 288, 339, 441, 498, 1316, 2382, 2394 |