DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 229 |
| Acc36I | ACCTGC | 1 cut(s) | 229 |
| AciI | CCGC | 1 cut(s) | 18 |
| AcuI | CTGAAG | 1 cut(s) | 111 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 128 |
| AgsI | TTSAA | 1 cut(s) | 14 |
| AjnI | CCWGG | 1 cut(s) | 204 |
| AluBI | AGCT | 1 cut(s) | 77 |
| AluI | AGCT | 1 cut(s) | 77 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 106 |
| AoxI | GGCC | 2 cut(s) | 7, 202 |
| AspS9I | GGNCC | 1 cut(s) | 7 |
| BccI | CCATC | 2 cut(s) | 146, 194 |
| BciT130I | CCWGG | 1 cut(s) | 206 |
| BfmI | CTRYAG | 1 cut(s) | 53 |
| BfuAI | ACCTGC | 1 cut(s) | 229 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 15 |
| Bme1390I | CCNGG | 1 cut(s) | 206 |
| BmgT120I | GGNCC | 1 cut(s) | 7 |
| BmiI | GGNNCC | 1 cut(s) | 120 |
| BmrFI | CCNGG | 1 cut(s) | 206 |
| BmsI | GCATC | 1 cut(s) | 93 |
| BpuEI | CTTGAG | 1 cut(s) | 146 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 128 |
| BseBI | CCWGG | 1 cut(s) | 206 |
| BseGI | GGATG | 1 cut(s) | 205 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BseMII | CTCAG | 1 cut(s) | 98 |
| BshFI | GGCC | 2 cut(s) | 9, 204 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 128 |
| BsnI | GGCC | 2 cut(s) | 9, 204 |
| BspACI | CCGC | 1 cut(s) | 18 |
| BspANI | GGCC | 2 cut(s) | 9, 204 |
| BspCNI | CTCAG | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 120 |
| BspMAI | CTGCAG | 1 cut(s) | 57 |
| BspMI | ACCTGC | 1 cut(s) | 229 |
| Bst2UI | CCWGG | 1 cut(s) | 206 |
| Bst4CI | ACNGT | 1 cut(s) | 36 |
| Bst6I | CTCTTC | 1 cut(s) | 208 |
| BstDEI | CTNAG | 1 cut(s) | 107 |
| BstF5I | GGATG | 1 cut(s) | 205 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 15 |
| BstNI | CCWGG | 1 cut(s) | 206 |
| BstSCI | CCNGG | 1 cut(s) | 204 |
| BstSFI | CTRYAG | 1 cut(s) | 53 |
| BsuRI | GGCC | 2 cut(s) | 9, 204 |
| BtsCI | GGATG | 1 cut(s) | 205 |
| BveI | ACCTGC | 1 cut(s) | 229 |
| CaiI | CAGNNNCTG | 1 cut(s) | 106 |
| Cfr13I | GGNCC | 1 cut(s) | 7 |
| CviAII | CATG | 2 cut(s) | 70, 234 |
| CviJI | RGCY | 3 cut(s) | 9, 77, 204 |
| CviKI_1 | RGCY | 3 cut(s) | 9, 77, 204 |
| DdeI | CTNAG | 1 cut(s) | 107 |
| Eam1104I | CTCTTC | 1 cut(s) | 208 |
| EarI | CTCTTC | 1 cut(s) | 208 |
| Eco57I | CTGAAG | 1 cut(s) | 111 |
| EcoO109I | RGGNCCY | 1 cut(s) | 7 |
| EcoRII | CCWGG | 1 cut(s) | 204 |
| EcoT22I | ATGCAT | 1 cut(s) | 235 |
| FaeI | CATG | 2 cut(s) | 73, 237 |
| FaiI | YATR | 5 cut(s) | 24, 39, 71, 231, 235 |
| FatI | CATG | 2 cut(s) | 69, 233 |
| FokI | GGATG | 1 cut(s) | 212 |
| HaeIII | GGCC | 2 cut(s) | 9, 204 |
| Hin1II | CATG | 2 cut(s) | 73, 237 |
| HinfI | GANTC | 1 cut(s) | 85 |
| Hpy166II | GTNNAC | 1 cut(s) | 32 |
| Hpy188I | TCNGA | 1 cut(s) | 123 |
| Hpy188III | TCNNGA | 2 cut(s) | 89, 149 |
| Hpy8I | GTNNAC | 1 cut(s) | 32 |
| HpyAV | CCTTC | 1 cut(s) | 8 |
| HpyCH4III | ACNGT | 1 cut(s) | 36 |
| HpyCH4V | TGCA | 3 cut(s) | 55, 194, 233 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 15 |
| HpyF3I | CTNAG | 1 cut(s) | 107 |
| Hsp92II | CATG | 2 cut(s) | 73, 237 |
| LpnPI | CCDG | 5 cut(s) | 65, 102, 191, 218, 224 |
| LweI | GCATC | 1 cut(s) | 93 |
| MboII | GAAGA | 3 cut(s) | 104, 188, 225 |
| MlyI | GAGTC | 1 cut(s) | 79 |
| MnlI | CCTC | 2 cut(s) | 37, 209 |
| Mph1103I | ATGCAT | 1 cut(s) | 235 |
| MspR9I | CCNGG | 1 cut(s) | 206 |
| MvaI | CCWGG | 1 cut(s) | 206 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 15 |
| NlaIII | CATG | 2 cut(s) | 73, 237 |
| NlaIV | GGNNCC | 1 cut(s) | 120 |
| NsiI | ATGCAT | 1 cut(s) | 235 |
| PaqCI | CACCTGC | 1 cut(s) | 229 |
| PleI | GAGTC | 1 cut(s) | 79 |
| PpsI | GAGTC | 1 cut(s) | 79 |
| Psp6I | CCWGG | 1 cut(s) | 204 |
| PspGI | CCWGG | 1 cut(s) | 204 |
| PspN4I | GGNNCC | 1 cut(s) | 120 |
| PspPI | GGNCC | 1 cut(s) | 7 |
| PstI | CTGCAG | 1 cut(s) | 57 |
| PstNI | CAGNNNCTG | 1 cut(s) | 106 |
| Sau96I | GGNCC | 1 cut(s) | 7 |
| SchI | GAGTC | 1 cut(s) | 79 |
| ScrFI | CCNGG | 1 cut(s) | 206 |
| SetI | ASST | 4 cut(s) | 48, 79, 120, 243 |
| SfaNI | GCATC | 1 cut(s) | 93 |
| SfcI | CTRYAG | 1 cut(s) | 53 |
| SgeI | CNNG | 9 cut(s) | 23, 64, 82, 101, 161, 175, 179, 217, 218 |
| SmlI | CTYRAG | 1 cut(s) | 161 |
| SmoI | CTYRAG | 1 cut(s) | 161 |
| SsiI | CCGC | 1 cut(s) | 18 |
| StyD4I | CCNGG | 1 cut(s) | 204 |
| TaaI | ACNGT | 1 cut(s) | 36 |
| Zsp2I | ATGCAT | 1 cut(s) | 235 |