DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1697 |
| AccB1I | GGYRCC | 2 cut(s) | 1702, 3176 |
| AccBSI | CCGCTC | 3 cut(s) | 577, 2714, 3200 |
| AccII | CGCG | 2 cut(s) | 69, 217 |
| AccIII | TCCGGA | 1 cut(s) | 2584 |
| AciI | CCGC | 8 cut(s) | 538, 577, 1345, 1828, 2714, 2906, 3200, 3328 |
| AclI | AACGTT | 1 cut(s) | 2510 |
| AclWI | GGATC | 7 cut(s) | 72, 531, 1019, 1612, 1662, 1723, 2268 |
| AcoI | YGGCCR | 3 cut(s) | 2000, 2488, 3201 |
| AcsI | RAATTY | 4 cut(s) | 1045, 1351, 1494, 2681 |
| AcuI | CTGAAG | 2 cut(s) | 186, 1083 |
| AfaI | GTAC | 6 cut(s) | 563, 895, 1761, 2460, 2476, 2869 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 61, 679, 1215, 1787, 1860, 2044, 2233, 2918, 2990 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2079, 2111 |
| Alw21I | GWGCWC | 2 cut(s) | 133, 3379 |
| Alw26I | GTCTC | 9 cut(s) | 114, 189, 354, 563, 1013, 1572, 1772, 2235, 2291 |
| AlwI | GGATC | 7 cut(s) | 72, 531, 1019, 1612, 1662, 1723, 2268 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3251 |
| Ama87I | CYCGRG | 2 cut(s) | 2639, 3024 |
| Aor13HI | TCCGGA | 1 cut(s) | 2584 |
| AoxI | GGCC | 7 cut(s) | 1682, 2000, 2301, 2479, 2488, 3201, 3283 |
| ApaI | GGGCCC | 1 cut(s) | 3287 |
| ApoI | RAATTY | 4 cut(s) | 1045, 1351, 1494, 2681 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1946, 1978 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 864, 1719 |
| AspLEI | GCGC | 1 cut(s) | 217 |
| AspS9I | GGNCC | 6 cut(s) | 1219, 1682, 2301, 3248, 3283, 3284 |
| AsuC2I | CCSGG | 1 cut(s) | 2258 |
| AsuHPI | GGTGA | 8 cut(s) | 44, 421, 1154, 1291, 1378, 2201, 2431, 3199 |
| AsuNHI | GCTAGC | 1 cut(s) | 3005 |
| AvaI | CYCGRG | 2 cut(s) | 2639, 3024 |
| AvaII | GGWCC | 2 cut(s) | 1219, 3248 |
| AxyI | CCTNAGG | 1 cut(s) | 874 |
| BaeGI | GKGCMC | 1 cut(s) | 3287 |
| BanI | GGYRCC | 2 cut(s) | 1702, 3176 |
| BanII | GRGCYC | 3 cut(s) | 632, 3287, 3379 |
| BbsI | GAAGAC | 1 cut(s) | 3345 |
| Bbv12I | GWGCWC | 2 cut(s) | 133, 3379 |
| BbvCI | CCTCAGC | 1 cut(s) | 1640 |
| BccI | CCATC | 6 cut(s) | 929, 1121, 1673, 3008, 3141, 3212 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 953, 987 |
| BciVI | GTATCC | 1 cut(s) | 2798 |
| BclI | TGATCA | 3 cut(s) | 511, 1315, 2421 |
| BcnI | CCSGG | 1 cut(s) | 2258 |
| BcoDI | GTCTC | 9 cut(s) | 114, 189, 354, 563, 1013, 1572, 1772, 2235, 2291 |
| BfaI | CTAG | 6 cut(s) | 1005, 1980, 2532, 2865, 3006, 3144 |
| BfmI | CTRYAG | 3 cut(s) | 1086, 1560, 3042 |
| BfuI | GTATCC | 1 cut(s) | 2798 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1690, 2487 |
| BglII | AGATCT | 2 cut(s) | 1374, 2545 |
| BlpI | GCTNAGC | 1 cut(s) | 3378 |
| Bme18I | GGWCC | 2 cut(s) | 1219, 3248 |
| BmeT110I | CYCGRG | 2 cut(s) | 2639, 3024 |
| BmgT120I | GGNCC | 6 cut(s) | 1219, 1682, 2301, 3248, 3283, 3284 |
| BmiI | GGNNCC | 5 cut(s) | 1220, 1704, 2302, 3178, 3285 |
| BmrI | ACTGGG | 1 cut(s) | 1772 |
| BmtI | GCTAGC | 1 cut(s) | 3009 |
| BmuI | ACTGGG | 1 cut(s) | 1772 |
| BpiI | GAAGAC | 1 cut(s) | 3345 |
| BpmI | CTGGAG | 4 cut(s) | 1695, 2526, 3216, 3273 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1548, 1640, 2267, 2727 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3378 |
| BpuEI | CTTGAG | 2 cut(s) | 1244, 1363 |
| BpuMI | CCSGG | 1 cut(s) | 2258 |
| BsaAI | YACGTR | 1 cut(s) | 2063 |
| BsaBI | GATNNNNATC | 2 cut(s) | 63, 447 |
| BsaI | GGTCTC | 2 cut(s) | 114, 1772 |
| BsaWI | WCCGGW | 1 cut(s) | 2584 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 577, 607 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 61, 679, 1215, 1787, 1860, 2044, 2233, 2918, 2990 |
| Bse1I | ACTGG | 9 cut(s) | 1483, 1488, 1606, 1767, 2227, 2421, 2662, 2995, 3199 |
| Bse21I | CCTNAGG | 1 cut(s) | 874 |
| Bse3DI | GCAATG | 2 cut(s) | 3016, 3096 |
| Bse8I | GATNNNNATC | 2 cut(s) | 63, 447 |
| BseAI | TCCGGA | 1 cut(s) | 2584 |
| BseJI | GATNNNNATC | 2 cut(s) | 63, 447 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 61, 679, 1215, 1787, 1860, 2044, 2233, 2918, 2990 |
| BseMI | GCAATG | 2 cut(s) | 3016, 3096 |
| BseNI | ACTGG | 9 cut(s) | 1483, 1488, 1606, 1767, 2227, 2421, 2662, 2995, 3199 |
| BseRI | GAGGAG | 9 cut(s) | 61, 472, 692, 695, 748, 1031, 1168, 2705, 2722 |
| BseSI | GKGCMC | 1 cut(s) | 3287 |
| BseYI | CCCAGC | 1 cut(s) | 1075 |
| BsgI | GTGCAG | 1 cut(s) | 957 |
| Bsh1236I | CGCG | 2 cut(s) | 69, 217 |
| BshFI | GGCC | 7 cut(s) | 1684, 2002, 2303, 2481, 2490, 3203, 3285 |
| BshNI | GGYRCC | 2 cut(s) | 1702, 3176 |
| BsiHKAI | GWGCWC | 2 cut(s) | 133, 3379 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2639, 3024 |
| BsiSI | CCGG | 4 cut(s) | 1411, 2257, 2491, 2585 |
| BslFI | GGGAC | 5 cut(s) | 892, 1232, 2420, 2649, 3001 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 61, 679, 1215, 1787, 1860, 2044, 2233, 2918, 2990 |
| BsmAI | GTCTC | 9 cut(s) | 114, 189, 354, 563, 1013, 1572, 1772, 2235, 2291 |
| BsmFI | GGGAC | 5 cut(s) | 892, 1232, 2420, 2649, 3001 |
| BsmI | GAATGC | 2 cut(s) | 3145, 3356 |
| BsnI | GGCC | 7 cut(s) | 1684, 2002, 2303, 2481, 2490, 3203, 3285 |
| Bso31I | GGTCTC | 2 cut(s) | 114, 1772 |
| BsoBI | CYCGRG | 2 cut(s) | 2639, 3024 |
| Bsp120I | GGGCCC | 1 cut(s) | 3283 |
| Bsp1286I | GDGCHC | 4 cut(s) | 133, 632, 3287, 3379 |
| Bsp13I | TCCGGA | 1 cut(s) | 2584 |
| Bsp1407I | TGTACA | 1 cut(s) | 2458 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3378 |
| Bsp19I | CCATGG | 1 cut(s) | 2650 |
| Bsp68I | TCGCGA | 1 cut(s) | 69 |
| BspACI | CCGC | 8 cut(s) | 538, 577, 1345, 1828, 2714, 2906, 3200, 3328 |
| BspANI | GGCC | 7 cut(s) | 1684, 2002, 2303, 2481, 2490, 3203, 3285 |
| BspEI | TCCGGA | 1 cut(s) | 2584 |
| BspFNI | CGCG | 2 cut(s) | 69, 217 |
| BspLI | GGNNCC | 5 cut(s) | 1220, 1704, 2302, 3178, 3285 |
| BspMAI | CTGCAG | 2 cut(s) | 1090, 3046 |
| BspOI | GCTAGC | 1 cut(s) | 3009 |
| BspPI | GGATC | 7 cut(s) | 72, 531, 1019, 1612, 1662, 1723, 2268 |
| BspT107I | GGYRCC | 2 cut(s) | 1702, 3176 |
| BspTNI | GGTCTC | 2 cut(s) | 114, 1772 |
| BsrBI | CCGCTC | 3 cut(s) | 577, 2714, 3200 |
| BsrDI | GCAATG | 2 cut(s) | 3016, 3096 |
| BsrGI | TGTACA | 1 cut(s) | 2458 |
| BsrI | ACTGG | 9 cut(s) | 1483, 1488, 1606, 1767, 2227, 2421, 2662, 2995, 3199 |
| BssT1I | CCWWGG | 3 cut(s) | 1755, 2650, 2947 |
| Bst4CI | ACNGT | 8 cut(s) | 125, 206, 1439, 2017, 2465, 2918, 2925, 3297 |
| Bst6I | CTCTTC | 7 cut(s) | 146, 251, 314, 373, 413, 1389, 2940 |
| BstAUI | TGTACA | 1 cut(s) | 2458 |
| BstBAI | YACGTR | 1 cut(s) | 2063 |
| BstC8I | GCNNGC | 6 cut(s) | 1408, 2488, 2646, 2820, 3007, 3263 |
| BstDSI | CCRYGG | 2 cut(s) | 124, 2650 |
| BstEII | GGTNACC | 1 cut(s) | 3154 |
| BstFNI | CGCG | 2 cut(s) | 69, 217 |
| BstHHI | GCGC | 1 cut(s) | 217 |
| BstMAI | GTCTC | 9 cut(s) | 114, 189, 354, 563, 1013, 1572, 1772, 2235, 2291 |
| BstNSI | RCATGY | 2 cut(s) | 806, 1410 |
| BstPI | GGTNACC | 1 cut(s) | 3154 |
| BstSFI | CTRYAG | 3 cut(s) | 1086, 1560, 3042 |
| BstSLI | GKGCMC | 1 cut(s) | 3287 |
| BstUI | CGCG | 2 cut(s) | 69, 217 |
| BstV2I | GAAGAC | 1 cut(s) | 3345 |
| BstX2I | RGATCY | 4 cut(s) | 1374, 1728, 2260, 2545 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1762, 2657 |
| BstYI | RGATCY | 4 cut(s) | 1374, 1728, 2260, 2545 |
| Bsu36I | CCTNAGG | 1 cut(s) | 874 |
| BsuI | GTATCC | 1 cut(s) | 2798 |
| BsuRI | GGCC | 7 cut(s) | 1684, 2002, 2303, 2481, 2490, 3203, 3285 |
| BtgI | CCRYGG | 2 cut(s) | 124, 2650 |
| BtsI | GCAGTG | 1 cut(s) | 1087 |
| BtsIMutI | CAGTG | 2 cut(s) | 211, 1087 |
| BtuMI | TCGCGA | 1 cut(s) | 69 |
| Cac8I | GCNNGC | 6 cut(s) | 1408, 2488, 2646, 2820, 3007, 3263 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3251 |
| CfoI | GCGC | 1 cut(s) | 217 |
| Cfr13I | GGNCC | 6 cut(s) | 1219, 1682, 2301, 3248, 3283, 3284 |
| CseI | GACGC | 1 cut(s) | 1100 |
| Csp6I | GTAC | 6 cut(s) | 562, 894, 1760, 2459, 2475, 2868 |
| CviQI | GTAC | 6 cut(s) | 562, 894, 1760, 2459, 2475, 2868 |
| DraI | TTTAAA | 1 cut(s) | 2680 |
| EaeI | YGGCCR | 3 cut(s) | 2000, 2488, 3201 |
| Eam1104I | CTCTTC | 7 cut(s) | 146, 251, 314, 373, 413, 1389, 2940 |
| EarI | CTCTTC | 7 cut(s) | 146, 251, 314, 373, 413, 1389, 2940 |
| Ecl136II | GAGCTC | 1 cut(s) | 3377 |
| Eco130I | CCWWGG | 3 cut(s) | 1755, 2650, 2947 |
| Eco24I | GRGCYC | 3 cut(s) | 632, 3287, 3379 |
| Eco31I | GGTCTC | 2 cut(s) | 114, 1772 |
| Eco32I | GATATC | 1 cut(s) | 3392 |
| Eco47I | GGWCC | 2 cut(s) | 1219, 3248 |
| Eco53kI | GAGCTC | 1 cut(s) | 3377 |
| Eco57I | CTGAAG | 2 cut(s) | 186, 1083 |
| Eco81I | CCTNAGG | 1 cut(s) | 874 |
| Eco88I | CYCGRG | 2 cut(s) | 2639, 3024 |
| Eco91I | GGTNACC | 1 cut(s) | 3154 |
| EcoICRI | GAGCTC | 1 cut(s) | 3377 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1219, 3248, 3283, 3284 |
| EcoO65I | GGTNACC | 1 cut(s) | 3154 |
| EcoRI | GAATTC | 1 cut(s) | 1045 |
| EcoRV | GATATC | 1 cut(s) | 3392 |
| EcoT14I | CCWWGG | 3 cut(s) | 1755, 2650, 2947 |
| EcoT22I | ATGCAT | 1 cut(s) | 3358 |
| EcoT38I | GRGCYC | 3 cut(s) | 632, 3287, 3379 |
| ErhI | CCWWGG | 3 cut(s) | 1755, 2650, 2947 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 903, 935, 3254, 3286 |
| FaqI | GGGAC | 5 cut(s) | 892, 1232, 2420, 2649, 3001 |
| FauI | CCCGC | 3 cut(s) | 570, 1821, 3321 |
| FauNDI | CATATG | 1 cut(s) | 609 |
| FbaI | TGATCA | 3 cut(s) | 511, 1315, 2421 |
| FriOI | GRGCYC | 3 cut(s) | 632, 3287, 3379 |
| FspBI | CTAG | 6 cut(s) | 1005, 1980, 2532, 2865, 3006, 3144 |
| GlaI | GCGC | 1 cut(s) | 216 |
| GsaI | CCCAGC | 1 cut(s) | 1079 |
| GsuI | CTGGAG | 4 cut(s) | 1695, 2526, 3216, 3273 |
| HaeIII | GGCC | 7 cut(s) | 1684, 2002, 2303, 2481, 2490, 3203, 3285 |
| HapII | CCGG | 4 cut(s) | 1411, 2257, 2491, 2585 |
| HgaI | GACGC | 1 cut(s) | 1100 |
| HhaI | GCGC | 1 cut(s) | 217 |
| Hin6I | GCGC | 1 cut(s) | 215 |
| HinP1I | GCGC | 1 cut(s) | 215 |
| HpaII | CCGG | 4 cut(s) | 1411, 2257, 2491, 2585 |
| HphI | GGTGA | 8 cut(s) | 44, 421, 1154, 1291, 1378, 2201, 2431, 3199 |
| Hpy166II | GTNNAC | 4 cut(s) | 1145, 2328, 2461, 2921 |
| Hpy8I | GTNNAC | 4 cut(s) | 1145, 2328, 2461, 2921 |
| Hpy99I | CGWCG | 2 cut(s) | 138, 1265 |
| HpyCH4III | ACNGT | 8 cut(s) | 125, 206, 1439, 2017, 2465, 2918, 2925, 3297 |
| HpyCH4IV | ACGT | 4 cut(s) | 136, 2062, 2473, 2510 |
| HpySE526I | ACGT | 4 cut(s) | 136, 2062, 2473, 2510 |
| HspAI | GCGC | 1 cut(s) | 215 |
| Kpn2I | TCCGGA | 1 cut(s) | 2584 |
| Ksp22I | TGATCA | 3 cut(s) | 511, 1315, 2421 |
| LmnI | GCTCC | 6 cut(s) | 424, 992, 2407, 2545, 3197, 3374 |
| MaeI | CTAG | 6 cut(s) | 1005, 1980, 2532, 2865, 3006, 3144 |
| MaeII | ACGT | 4 cut(s) | 136, 2062, 2473, 2510 |
| MaeIII | GTNAC | 4 cut(s) | 50, 1915, 2626, 3154 |
| MbiI | CCGCTC | 3 cut(s) | 577, 2714, 3200 |
| MfeI | CAATTG | 1 cut(s) | 1418 |
| MflI | RGATCY | 4 cut(s) | 1374, 1728, 2260, 2545 |
| MhlI | GDGCHC | 4 cut(s) | 133, 632, 3287, 3379 |
| MlyI | GAGTC | 5 cut(s) | 58, 829, 1396, 2333, 2631 |
| MmeI | TCCRAC | 3 cut(s) | 2052, 2449, 3096 |
| Mph1103I | ATGCAT | 1 cut(s) | 3358 |
| MroI | TCCGGA | 1 cut(s) | 2584 |
| MroXI | GAANNNNTTC | 2 cut(s) | 864, 1719 |
| MslI | CAYNNNNRTG | 4 cut(s) | 486, 546, 807, 2277 |
| MspA1I | CMGCKG | 1 cut(s) | 971 |
| MspI | CCGG | 4 cut(s) | 1411, 2257, 2491, 2585 |
| MunI | CAATTG | 1 cut(s) | 1418 |
| Mva1269I | GAATGC | 2 cut(s) | 3145, 3356 |
| MvnI | CGCG | 2 cut(s) | 69, 217 |
| NciI | CCSGG | 1 cut(s) | 2258 |
| NcoI | CCATGG | 1 cut(s) | 2650 |
| NdeI | CATATG | 1 cut(s) | 609 |
| NheI | GCTAGC | 1 cut(s) | 3005 |
| NlaIV | GGNNCC | 5 cut(s) | 1220, 1704, 2302, 3178, 3285 |
| NmeAIII | GCCGAG | 2 cut(s) | 248, 2507 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| NruI | TCGCGA | 1 cut(s) | 69 |
| NsiI | ATGCAT | 1 cut(s) | 3358 |
| NspI | RCATGY | 2 cut(s) | 806, 1410 |
| PaeI | GCATGC | 1 cut(s) | 1410 |
| PaeR7I | CTCGAG | 1 cut(s) | 2639 |
| PasI | CCCWGGG | 1 cut(s) | 2984 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2005, 2479 |
| PctI | GAATGC | 2 cut(s) | 3145, 3356 |
| PdmI | GAANNNNTTC | 2 cut(s) | 864, 1719 |
| PfoI | TCCNGGA | 1 cut(s) | 2256 |
| PleI | GAGTC | 5 cut(s) | 57, 829, 1395, 2332, 2631 |
| PpsI | GAGTC | 5 cut(s) | 57, 829, 1395, 2332, 2631 |
| Ppu21I | YACGTR | 1 cut(s) | 2063 |
| PpuMI | RGGWCCY | 2 cut(s) | 1219, 3248 |
| PsiI | TTATAA | 1 cut(s) | 1697 |
| Psp124BI | GAGCTC | 1 cut(s) | 3379 |
| Psp1406I | AACGTT | 1 cut(s) | 2510 |
| Psp5II | RGGWCCY | 2 cut(s) | 1219, 3248 |
| PspEI | GGTNACC | 1 cut(s) | 3154 |
| PspFI | CCCAGC | 1 cut(s) | 1075 |
| PspN4I | GGNNCC | 5 cut(s) | 1220, 1704, 2302, 3178, 3285 |
| PspOMI | GGGCCC | 1 cut(s) | 3283 |
| PspPI | GGNCC | 6 cut(s) | 1219, 1682, 2301, 3248, 3283, 3284 |
| PspPPI | RGGWCCY | 2 cut(s) | 1219, 3248 |
| PspXI | VCTCGAGB | 1 cut(s) | 2639 |
| PstI | CTGCAG | 2 cut(s) | 1090, 3046 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3251 |
| PsuI | RGATCY | 4 cut(s) | 1374, 1728, 2260, 2545 |
| PvuII | CAGCTG | 1 cut(s) | 971 |
| RruI | TCGCGA | 1 cut(s) | 69 |
| RsaI | GTAC | 6 cut(s) | 563, 895, 1761, 2460, 2476, 2869 |
| RsaNI | GTAC | 6 cut(s) | 562, 894, 1760, 2459, 2475, 2868 |
| RseI | CAYNNNNRTG | 4 cut(s) | 486, 546, 807, 2277 |
| SacI | GAGCTC | 1 cut(s) | 3379 |
| Sau96I | GGNCC | 6 cut(s) | 1219, 1682, 2301, 3248, 3283, 3284 |
| SchI | GAGTC | 5 cut(s) | 58, 829, 1396, 2333, 2631 |
| SduI | GDGCHC | 4 cut(s) | 133, 632, 3287, 3379 |
| SfcI | CTRYAG | 3 cut(s) | 1086, 1560, 3042 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2487 |
| Sfr274I | CTCGAG | 1 cut(s) | 2639 |
| SinI | GGWCC | 2 cut(s) | 1219, 3248 |
| SlaI | CTCGAG | 1 cut(s) | 2639 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 486, 546, 807, 2277 |
| SmlI | CTYRAG | 3 cut(s) | 1223, 1378, 2639 |
| SmoI | CTYRAG | 3 cut(s) | 1223, 1378, 2639 |
| SphI | GCATGC | 1 cut(s) | 1410 |
| SsiI | CCGC | 8 cut(s) | 538, 577, 1345, 1828, 2714, 2906, 3200, 3328 |
| SspI | AATATT | 1 cut(s) | 1996 |
| SspMI | CTAG | 6 cut(s) | 1005, 1980, 2532, 2865, 3006, 3144 |
| SstI | GAGCTC | 1 cut(s) | 3379 |
| StyI | CCWWGG | 3 cut(s) | 1755, 2650, 2947 |
| TaaI | ACNGT | 8 cut(s) | 125, 206, 1439, 2017, 2465, 2918, 2925, 3297 |
| TaiI | ACGT | 4 cut(s) | 139, 2065, 2476, 2513 |
| TaqI | TCGA | 9 cut(s) | 23, 133, 193, 451, 1132, 1576, 1945, 2640, 3279 |
| TatI | WGTACW | 3 cut(s) | 561, 2458, 2867 |
| TauI | GCSGC | 3 cut(s) | 541, 2717, 3203 |
| TscAI | CASTG | 2 cut(s) | 211, 1087 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspGWI | ACGGA | 1 cut(s) | 1017 |
| TspRI | CASTG | 2 cut(s) | 211, 1087 |
| VpaK11BI | GGWCC | 2 cut(s) | 1219, 3248 |
| XapI | RAATTY | 4 cut(s) | 1045, 1351, 1494, 2681 |
| XceI | RCATGY | 2 cut(s) | 806, 1410 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1495 |
| XhoI | CTCGAG | 1 cut(s) | 2639 |
| XmnI | GAANNNNTTC | 2 cut(s) | 864, 1719 |
| XspI | CTAG | 6 cut(s) | 1005, 1980, 2532, 2865, 3006, 3144 |
| Zsp2I | ATGCAT | 1 cut(s) | 3358 |