DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1703 |
| AarI | CACCTGC | 1 cut(s) | 3542 |
| Acc36I | ACCTGC | 1 cut(s) | 3542 |
| AccB1I | GGYRCC | 2 cut(s) | 1708, 3182 |
| AccBSI | CCGCTC | 3 cut(s) | 583, 2720, 3206 |
| AccII | CGCG | 3 cut(s) | 69, 217, 2068 |
| AccIII | TCCGGA | 1 cut(s) | 2590 |
| AciI | CCGC | 9 cut(s) | 544, 583, 1014, 1351, 1834, 2720, 2912, 3206, 3334 |
| AclI | AACGTT | 1 cut(s) | 2516 |
| AclWI | GGATC | 7 cut(s) | 72, 537, 1025, 1618, 1668, 1729, 2274 |
| AcoI | YGGCCR | 3 cut(s) | 2006, 2494, 3207 |
| AcsI | RAATTY | 5 cut(s) | 1051, 1357, 1500, 2687, 3389 |
| AcuI | CTGAAG | 2 cut(s) | 186, 1089 |
| AfaI | GTAC | 6 cut(s) | 569, 901, 1767, 2466, 2482, 2875 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2085, 2117 |
| Alw21I | GWGCWC | 1 cut(s) | 133 |
| Alw26I | GTCTC | 8 cut(s) | 114, 189, 354, 569, 1019, 1578, 1778, 2297 |
| AlwI | GGATC | 7 cut(s) | 72, 537, 1025, 1618, 1668, 1729, 2274 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3257 |
| Ama87I | CYCGRG | 2 cut(s) | 2645, 3030 |
| Aor13HI | TCCGGA | 1 cut(s) | 2590 |
| AoxI | GGCC | 8 cut(s) | 1688, 2006, 2307, 2485, 2494, 3207, 3289, 3402 |
| ApaI | GGGCCC | 1 cut(s) | 3293 |
| ApoI | RAATTY | 5 cut(s) | 1051, 1357, 1500, 2687, 3389 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1952, 1984 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 870, 1725 |
| AspLEI | GCGC | 2 cut(s) | 217, 2068 |
| AspS9I | GGNCC | 8 cut(s) | 1225, 1688, 2307, 3254, 3289, 3290, 3402, 3433 |
| AsuC2I | CCSGG | 1 cut(s) | 2264 |
| AsuHPI | GGTGA | 8 cut(s) | 44, 427, 1160, 1297, 1384, 2207, 2437, 3205 |
| AsuNHI | GCTAGC | 2 cut(s) | 1010, 3011 |
| AvaI | CYCGRG | 2 cut(s) | 2645, 3030 |
| AvaII | GGWCC | 3 cut(s) | 1225, 3254, 3433 |
| AxyI | CCTNAGG | 1 cut(s) | 880 |
| BaeGI | GKGCMC | 1 cut(s) | 3293 |
| BanI | GGYRCC | 2 cut(s) | 1708, 3182 |
| BanII | GRGCYC | 2 cut(s) | 638, 3293 |
| BbsI | GAAGAC | 1 cut(s) | 3351 |
| Bbv12I | GWGCWC | 1 cut(s) | 133 |
| BbvCI | CCTCAGC | 1 cut(s) | 1646 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 959, 993 |
| BciVI | GTATCC | 1 cut(s) | 2804 |
| BclI | TGATCA | 3 cut(s) | 517, 1321, 2427 |
| BcnI | CCSGG | 1 cut(s) | 2264 |
| BcoDI | GTCTC | 8 cut(s) | 114, 189, 354, 569, 1019, 1578, 1778, 2297 |
| BfaI | CTAG | 6 cut(s) | 1011, 1986, 2538, 2871, 3012, 3150 |
| BfmI | CTRYAG | 3 cut(s) | 1092, 1566, 3048 |
| BfuAI | ACCTGC | 1 cut(s) | 3542 |
| BfuI | GTATCC | 1 cut(s) | 2804 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1696, 2493 |
| BglII | AGATCT | 2 cut(s) | 1380, 2551 |
| Bme18I | GGWCC | 3 cut(s) | 1225, 3254, 3433 |
| BmeT110I | CYCGRG | 2 cut(s) | 2645, 3030 |
| BmgT120I | GGNCC | 8 cut(s) | 1225, 1688, 2307, 3254, 3289, 3290, 3402, 3433 |
| BmiI | GGNNCC | 6 cut(s) | 1226, 1710, 2308, 3184, 3291, 3403 |
| BmrI | ACTGGG | 1 cut(s) | 1778 |
| BmtI | GCTAGC | 2 cut(s) | 1014, 3015 |
| BmuI | ACTGGG | 1 cut(s) | 1778 |
| BpiI | GAAGAC | 1 cut(s) | 3351 |
| BpmI | CTGGAG | 4 cut(s) | 1701, 2532, 3222, 3279 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1554, 1646, 2273, 2733 |
| BpuEI | CTTGAG | 2 cut(s) | 1250, 1369 |
| BpuMI | CCSGG | 1 cut(s) | 2264 |
| BsaBI | GATNNNNATC | 2 cut(s) | 63, 453 |
| BsaI | GGTCTC | 2 cut(s) | 114, 1778 |
| BsaWI | WCCGGW | 1 cut(s) | 2590 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 583, 613 |
| Bse21I | CCTNAGG | 1 cut(s) | 880 |
| Bse3DI | GCAATG | 2 cut(s) | 3022, 3102 |
| Bse8I | GATNNNNATC | 2 cut(s) | 63, 453 |
| BseAI | TCCGGA | 1 cut(s) | 2590 |
| BseJI | GATNNNNATC | 2 cut(s) | 63, 453 |
| BseMI | GCAATG | 2 cut(s) | 3022, 3102 |
| BseSI | GKGCMC | 1 cut(s) | 3293 |
| BseYI | CCCAGC | 1 cut(s) | 1081 |
| BsgI | GTGCAG | 1 cut(s) | 963 |
| Bsh1236I | CGCG | 3 cut(s) | 69, 217, 2068 |
| BshFI | GGCC | 8 cut(s) | 1690, 2008, 2309, 2487, 2496, 3209, 3291, 3404 |
| BshNI | GGYRCC | 2 cut(s) | 1708, 3182 |
| BsiHKAI | GWGCWC | 1 cut(s) | 133 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2645, 3030 |
| BsiSI | CCGG | 4 cut(s) | 1417, 2263, 2497, 2591 |
| BslFI | GGGAC | 6 cut(s) | 898, 1238, 2221, 2426, 2655, 3007 |
| BsmAI | GTCTC | 8 cut(s) | 114, 189, 354, 569, 1019, 1578, 1778, 2297 |
| BsmFI | GGGAC | 6 cut(s) | 898, 1238, 2221, 2426, 2655, 3007 |
| BsmI | GAATGC | 2 cut(s) | 3151, 3362 |
| BsnI | GGCC | 8 cut(s) | 1690, 2008, 2309, 2487, 2496, 3209, 3291, 3404 |
| Bso31I | GGTCTC | 2 cut(s) | 114, 1778 |
| BsoBI | CYCGRG | 2 cut(s) | 2645, 3030 |
| Bsp120I | GGGCCC | 1 cut(s) | 3289 |
| Bsp1286I | GDGCHC | 3 cut(s) | 133, 638, 3293 |
| Bsp13I | TCCGGA | 1 cut(s) | 2590 |
| Bsp1407I | TGTACA | 1 cut(s) | 2464 |
| Bsp19I | CCATGG | 1 cut(s) | 2656 |
| Bsp68I | TCGCGA | 1 cut(s) | 69 |
| BspACI | CCGC | 9 cut(s) | 544, 583, 1014, 1351, 1834, 2720, 2912, 3206, 3334 |
| BspANI | GGCC | 8 cut(s) | 1690, 2008, 2309, 2487, 2496, 3209, 3291, 3404 |
| BspEI | TCCGGA | 1 cut(s) | 2590 |
| BspFNI | CGCG | 3 cut(s) | 69, 217, 2068 |
| BspLI | GGNNCC | 6 cut(s) | 1226, 1710, 2308, 3184, 3291, 3403 |
| BspMAI | CTGCAG | 2 cut(s) | 1096, 3052 |
| BspMI | ACCTGC | 1 cut(s) | 3542 |
| BspOI | GCTAGC | 2 cut(s) | 1014, 3015 |
| BspPI | GGATC | 7 cut(s) | 72, 537, 1025, 1618, 1668, 1729, 2274 |
| BspT107I | GGYRCC | 2 cut(s) | 1708, 3182 |
| BspTNI | GGTCTC | 2 cut(s) | 114, 1778 |
| BsrBI | CCGCTC | 3 cut(s) | 583, 2720, 3206 |
| BsrDI | GCAATG | 2 cut(s) | 3022, 3102 |
| BsrGI | TGTACA | 1 cut(s) | 2464 |
| BssT1I | CCWWGG | 3 cut(s) | 1761, 2656, 2953 |
| Bst6I | CTCTTC | 7 cut(s) | 146, 251, 314, 379, 419, 1395, 2946 |
| BstAUI | TGTACA | 1 cut(s) | 2464 |
| BstC8I | GCNNGC | 7 cut(s) | 1012, 1414, 2494, 2652, 2826, 3013, 3269 |
| BstDSI | CCRYGG | 2 cut(s) | 124, 2656 |
| BstEII | GGTNACC | 1 cut(s) | 3160 |
| BstFNI | CGCG | 3 cut(s) | 69, 217, 2068 |
| BstHHI | GCGC | 2 cut(s) | 217, 2068 |
| BstMAI | GTCTC | 8 cut(s) | 114, 189, 354, 569, 1019, 1578, 1778, 2297 |
| BstNSI | RCATGY | 2 cut(s) | 812, 1416 |
| BstPI | GGTNACC | 1 cut(s) | 3160 |
| BstSFI | CTRYAG | 3 cut(s) | 1092, 1566, 3048 |
| BstSLI | GKGCMC | 1 cut(s) | 3293 |
| BstUI | CGCG | 3 cut(s) | 69, 217, 2068 |
| BstV2I | GAAGAC | 1 cut(s) | 3351 |
| BstX2I | RGATCY | 4 cut(s) | 1380, 1734, 2266, 2551 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1768, 2663 |
| BstYI | RGATCY | 4 cut(s) | 1380, 1734, 2266, 2551 |
| Bsu36I | CCTNAGG | 1 cut(s) | 880 |
| BsuI | GTATCC | 1 cut(s) | 2804 |
| BsuRI | GGCC | 8 cut(s) | 1690, 2008, 2309, 2487, 2496, 3209, 3291, 3404 |
| BtgI | CCRYGG | 2 cut(s) | 124, 2656 |
| BtsI | GCAGTG | 1 cut(s) | 1093 |
| BtsIMutI | CAGTG | 4 cut(s) | 211, 1093, 1747, 3420 |
| BtuMI | TCGCGA | 1 cut(s) | 69 |
| BveI | ACCTGC | 1 cut(s) | 3542 |
| Cac8I | GCNNGC | 7 cut(s) | 1012, 1414, 2494, 2652, 2826, 3013, 3269 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3257 |
| CfoI | GCGC | 2 cut(s) | 217, 2068 |
| Cfr13I | GGNCC | 8 cut(s) | 1225, 1688, 2307, 3254, 3289, 3290, 3402, 3433 |
| Csp6I | GTAC | 6 cut(s) | 568, 900, 1766, 2465, 2481, 2874 |
| CviQI | GTAC | 6 cut(s) | 568, 900, 1766, 2465, 2481, 2874 |
| DraI | TTTAAA | 1 cut(s) | 2686 |
| EaeI | YGGCCR | 3 cut(s) | 2006, 2494, 3207 |
| Eam1104I | CTCTTC | 7 cut(s) | 146, 251, 314, 379, 419, 1395, 2946 |
| EarI | CTCTTC | 7 cut(s) | 146, 251, 314, 379, 419, 1395, 2946 |
| Eco130I | CCWWGG | 3 cut(s) | 1761, 2656, 2953 |
| Eco24I | GRGCYC | 2 cut(s) | 638, 3293 |
| Eco31I | GGTCTC | 2 cut(s) | 114, 1778 |
| Eco47I | GGWCC | 3 cut(s) | 1225, 3254, 3433 |
| Eco57I | CTGAAG | 2 cut(s) | 186, 1089 |
| Eco81I | CCTNAGG | 1 cut(s) | 880 |
| Eco88I | CYCGRG | 2 cut(s) | 2645, 3030 |
| Eco91I | GGTNACC | 1 cut(s) | 3160 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1225, 3254, 3289, 3290 |
| EcoO65I | GGTNACC | 1 cut(s) | 3160 |
| EcoRI | GAATTC | 1 cut(s) | 1051 |
| EcoT14I | CCWWGG | 3 cut(s) | 1761, 2656, 2953 |
| EcoT22I | ATGCAT | 2 cut(s) | 1102, 3364 |
| EcoT38I | GRGCYC | 2 cut(s) | 638, 3293 |
| ErhI | CCWWGG | 3 cut(s) | 1761, 2656, 2953 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 909, 941, 3260, 3292 |
| FaqI | GGGAC | 6 cut(s) | 898, 1238, 2221, 2426, 2655, 3007 |
| FauI | CCCGC | 4 cut(s) | 576, 1007, 1827, 3327 |
| FauNDI | CATATG | 1 cut(s) | 615 |
| FbaI | TGATCA | 3 cut(s) | 517, 1321, 2427 |
| FriOI | GRGCYC | 2 cut(s) | 638, 3293 |
| FspBI | CTAG | 6 cut(s) | 1011, 1986, 2538, 2871, 3012, 3150 |
| GlaI | GCGC | 2 cut(s) | 216, 2067 |
| GsaI | CCCAGC | 1 cut(s) | 1085 |
| GsuI | CTGGAG | 4 cut(s) | 1701, 2532, 3222, 3279 |
| HaeIII | GGCC | 8 cut(s) | 1690, 2008, 2309, 2487, 2496, 3209, 3291, 3404 |
| HapII | CCGG | 4 cut(s) | 1417, 2263, 2497, 2591 |
| HhaI | GCGC | 2 cut(s) | 217, 2068 |
| Hin6I | GCGC | 2 cut(s) | 215, 2066 |
| HinP1I | GCGC | 2 cut(s) | 215, 2066 |
| HpaII | CCGG | 4 cut(s) | 1417, 2263, 2497, 2591 |
| HphI | GGTGA | 8 cut(s) | 44, 427, 1160, 1297, 1384, 2207, 2437, 3205 |
| Hpy166II | GTNNAC | 4 cut(s) | 1151, 2334, 2467, 2927 |
| Hpy8I | GTNNAC | 4 cut(s) | 1151, 2334, 2467, 2927 |
| Hpy99I | CGWCG | 2 cut(s) | 138, 1271 |
| HpyCH4IV | ACGT | 3 cut(s) | 136, 2479, 2516 |
| HpySE526I | ACGT | 3 cut(s) | 136, 2479, 2516 |
| HspAI | GCGC | 2 cut(s) | 215, 2066 |
| Kpn2I | TCCGGA | 1 cut(s) | 2590 |
| Ksp22I | TGATCA | 3 cut(s) | 517, 1321, 2427 |
| LmnI | GCTCC | 5 cut(s) | 430, 998, 2413, 2551, 3203 |
| MaeI | CTAG | 6 cut(s) | 1011, 1986, 2538, 2871, 3012, 3150 |
| MaeII | ACGT | 3 cut(s) | 136, 2479, 2516 |
| MaeIII | GTNAC | 5 cut(s) | 50, 1921, 2632, 3160, 3426 |
| MbiI | CCGCTC | 3 cut(s) | 583, 2720, 3206 |
| MfeI | CAATTG | 1 cut(s) | 1424 |
| MflI | RGATCY | 4 cut(s) | 1380, 1734, 2266, 2551 |
| MhlI | GDGCHC | 3 cut(s) | 133, 638, 3293 |
| MlyI | GAGTC | 5 cut(s) | 58, 835, 1402, 2339, 2637 |
| MmeI | TCCRAC | 3 cut(s) | 2058, 2455, 3102 |
| Mph1103I | ATGCAT | 2 cut(s) | 1102, 3364 |
| MroI | TCCGGA | 1 cut(s) | 2590 |
| MroXI | GAANNNNTTC | 2 cut(s) | 870, 1725 |
| MslI | CAYNNNNRTG | 5 cut(s) | 492, 552, 618, 813, 2283 |
| MspA1I | CMGCKG | 1 cut(s) | 977 |
| MspI | CCGG | 4 cut(s) | 1417, 2263, 2497, 2591 |
| MunI | CAATTG | 1 cut(s) | 1424 |
| Mva1269I | GAATGC | 2 cut(s) | 3151, 3362 |
| MvnI | CGCG | 3 cut(s) | 69, 217, 2068 |
| NciI | CCSGG | 1 cut(s) | 2264 |
| NcoI | CCATGG | 1 cut(s) | 2656 |
| NdeI | CATATG | 1 cut(s) | 615 |
| NheI | GCTAGC | 2 cut(s) | 1010, 3011 |
| NlaIV | GGNNCC | 6 cut(s) | 1226, 1710, 2308, 3184, 3291, 3403 |
| NmeAIII | GCCGAG | 2 cut(s) | 248, 2513 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| NruI | TCGCGA | 1 cut(s) | 69 |
| NsiI | ATGCAT | 2 cut(s) | 1102, 3364 |
| NspI | RCATGY | 2 cut(s) | 812, 1416 |
| PaeI | GCATGC | 1 cut(s) | 1416 |
| PaeR7I | CTCGAG | 1 cut(s) | 2645 |
| PaqCI | CACCTGC | 1 cut(s) | 3542 |
| PasI | CCCWGGG | 1 cut(s) | 2990 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2011, 2485 |
| PctI | GAATGC | 2 cut(s) | 3151, 3362 |
| PdmI | GAANNNNTTC | 2 cut(s) | 870, 1725 |
| PfoI | TCCNGGA | 1 cut(s) | 2262 |
| PleI | GAGTC | 5 cut(s) | 57, 835, 1401, 2338, 2637 |
| PpsI | GAGTC | 5 cut(s) | 57, 835, 1401, 2338, 2637 |
| PpuMI | RGGWCCY | 2 cut(s) | 1225, 3254 |
| PsiI | TTATAA | 1 cut(s) | 1703 |
| Psp1406I | AACGTT | 1 cut(s) | 2516 |
| Psp5II | RGGWCCY | 2 cut(s) | 1225, 3254 |
| PspEI | GGTNACC | 1 cut(s) | 3160 |
| PspFI | CCCAGC | 1 cut(s) | 1081 |
| PspN4I | GGNNCC | 6 cut(s) | 1226, 1710, 2308, 3184, 3291, 3403 |
| PspOMI | GGGCCC | 1 cut(s) | 3289 |
| PspPI | GGNCC | 8 cut(s) | 1225, 1688, 2307, 3254, 3289, 3290, 3402, 3433 |
| PspPPI | RGGWCCY | 2 cut(s) | 1225, 3254 |
| PspXI | VCTCGAGB | 1 cut(s) | 2645 |
| PstI | CTGCAG | 2 cut(s) | 1096, 3052 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3257 |
| PsuI | RGATCY | 4 cut(s) | 1380, 1734, 2266, 2551 |
| PvuII | CAGCTG | 1 cut(s) | 977 |
| RruI | TCGCGA | 1 cut(s) | 69 |
| RsaI | GTAC | 6 cut(s) | 569, 901, 1767, 2466, 2482, 2875 |
| RsaNI | GTAC | 6 cut(s) | 568, 900, 1766, 2465, 2481, 2874 |
| RseI | CAYNNNNRTG | 5 cut(s) | 492, 552, 618, 813, 2283 |
| Sau96I | GGNCC | 8 cut(s) | 1225, 1688, 2307, 3254, 3289, 3290, 3402, 3433 |
| SchI | GAGTC | 5 cut(s) | 58, 835, 1402, 2339, 2637 |
| SduI | GDGCHC | 3 cut(s) | 133, 638, 3293 |
| SfcI | CTRYAG | 3 cut(s) | 1092, 1566, 3048 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2493 |
| Sfr274I | CTCGAG | 1 cut(s) | 2645 |
| SinI | GGWCC | 3 cut(s) | 1225, 3254, 3433 |
| SlaI | CTCGAG | 1 cut(s) | 2645 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 492, 552, 618, 813, 2283 |
| SmlI | CTYRAG | 3 cut(s) | 1229, 1384, 2645 |
| SmoI | CTYRAG | 3 cut(s) | 1229, 1384, 2645 |
| SphI | GCATGC | 1 cut(s) | 1416 |
| SsiI | CCGC | 9 cut(s) | 544, 583, 1014, 1351, 1834, 2720, 2912, 3206, 3334 |
| SspI | AATATT | 1 cut(s) | 2002 |
| SspMI | CTAG | 6 cut(s) | 1011, 1986, 2538, 2871, 3012, 3150 |
| StyI | CCWWGG | 3 cut(s) | 1761, 2656, 2953 |
| TaiI | ACGT | 3 cut(s) | 139, 2482, 2519 |
| TatI | WGTACW | 3 cut(s) | 567, 2464, 2873 |
| TauI | GCSGC | 3 cut(s) | 547, 2723, 3209 |
| TscAI | CASTG | 4 cut(s) | 211, 1093, 1747, 3427 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspGWI | ACGGA | 1 cut(s) | 1023 |
| TspRI | CASTG | 4 cut(s) | 211, 1093, 1747, 3427 |
| VpaK11BI | GGWCC | 3 cut(s) | 1225, 3254, 3433 |
| XapI | RAATTY | 5 cut(s) | 1051, 1357, 1500, 2687, 3389 |
| XceI | RCATGY | 2 cut(s) | 812, 1416 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1501 |
| XhoI | CTCGAG | 1 cut(s) | 2645 |
| XmnI | GAANNNNTTC | 2 cut(s) | 870, 1725 |
| XspI | CTAG | 6 cut(s) | 1011, 1986, 2538, 2871, 3012, 3150 |
| Zsp2I | ATGCAT | 2 cut(s) | 1102, 3364 |