Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000906

Physical Location & Seq

Genomic Coordinates Forward (+)

6161809 .. 6166846

Gene Structure 5038 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1086200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3522 bp

ATGGTAGAGGGGGTCAAACATAAACCTAGGAAGGAGCGAGAATCGAAAAGGATTAGCGGTCATCGAGATGGCGAAAAGGAGGAGAATGGAGAGAGGAGACATCGGGGAAAAGATAGAGATTTTAAGAGAGATGGTGATTCTAGTCGTTCCGCGAGAGACAAGTTAGATAGAGATGAGAGAGATTGTGATTCTAGTCGTTCCACGAGAGACAAGTTTTGTGATTCTGAGGACGAGAGGTATGAGGTAGAGAAGCGGCATCGAGGAGATAGGGAGAGCGGTAGTCATAGGAGGGCTAGAGATAAAGAGATGGGGAGGGAGAAAGACAAAGAGAGGGGGAAAAGAGAGAGGGTTAGAGAGAAGGAAAGGGACAGAGATCGAAAGAGAAGGGTGCGGAGTGATGATGTAGATGAAGAGGAGGAGGAGGATGTTAGGGAGAGTGATAGGAAGCGGCGTAGGAAAGATGACAAGGAGAATGCGGAGAGAGTGAGGGAAAGGAGTAACAGCAGGTCAGATAGACATAGGGATGGTCGAGTTGAGAGCCCGGTAAAGAAGAGTGATGAAGATGATTCAGTTAGGAAAGGGACGAATCCGATCTCTGAGGTGGTGGAGGAGGATTTAGAGGATGAGCAGAGGAAATTAGATGAGGAAATGGAAAAGCGGAGGAGGAGAGTTCAGGAGTGGCAGGAGTTAAGAAGAAAGGAGAAACAAGCTGAGGGAGAGAAGCATGTGGAAGGGGATGTTGATGAACACAAGTCTAAAAAGACCTGGACACTTGAGGGAGAATCTGACGATGAAGAAGAGACAGGTATGGACCTTGACGGAGAAGCCTATCTCACTGATAAAGAGGATGGAGCCGTCATGGAGGTTGATTCTGAGAATCAAACAGCTGCACCTGCTTTGCAGGATGATGAAATTGATCCATTAGATGCTTTTATGAATTCTACGGTGCTGCCTGAAGTTGAGAAGCTGGCCAATGCTGCAGAACAACCAGTAGTTGCTGGTGAAAAAGGTTCAAACAATTCTTTGGGCAGAATAATTCCTGGGGAGTATTCTGATTCAGATTATGGGGACATTGAGAATGATGATGATCCCTTAGAAGACGAAGACGATGATGAGTTCACAAAAAGGGTGACAAAGACAAAAGTCGATAAACTTTCTCTAGTGGATCACTCAAAGATAGATTATGAACCATTCAGGAAAAATTTCTACATTGAGGTGAAGGAGGTCTCAAAAATGACCCCAGAAGATGTGGGTGCATACCGAAAACAATTGGAATTGAAGATACATGGTAAGGATGTCCCAAAGCCCATTAAGACCTGGCACCAAACTGGACTTACAAACAAAATCTTGGAAATGATAAAGAAGCTTGACTATGAAAAGCCAATGCCAATTCAGGCTCAGGCAGTGCCTATAATTATGAGTGGACGAGACTGCATTGGCATTGCAAAAACTGGCTCAGGCAAAACCCTTGCATATGTGCTGCCAATGCTGAGGCATATCAAGGACCAGCCACCTGTGGTAGCTGGAGATGGGCCTATTGGCCTTATAATGGCACCAACGAGGGAGCTTGTTCAGCAGATTCACAGTGTTATAAACAAGTTTGCCAAGACACTGGGTCTCAGGTGTGTGCCTGTATATGGAGGTTCTGGTATTGCCCAACAGATTGGGGAGTTGAAGCGGGGTGCTGCAATTGTCGTCTGTACCCCCGGTAGGATGATTGACATACTTTGTACAAATGGAGGGAGGATAACAAATCTGCAGAGAGTAACTTACTTGGTTGTGGATGAAGCTGATCGAATGTTTGACTTGGGTTTTGAACCTCAAATCAATCGAATTGTTCAGAATATTCGGCCAGATCATCAGACTCTATTGTTTTCTGCCACGTTCCCTCACCAGGTTGAAGTCTTAGCACGTAAAGTCTTAAACAAACCTGTGCATATACAGGTTGGTGGTAAGAGTGTTGTAAACAAGGACATTACACAATTTGTTGAAGTGAGGTCCGAAAATGAAAGGTTTGTTAGATTATTAGAGATACTTGGGGAATGGTACGAGAGAGGAAAAATTCTGGTGTTTGCCAAAGCACATGCAACGTGTGACACTGTGCTTAGGGATCTGCTTAGGCATGGATATCCTTGTCTCTCGCTTCATGGGAACAAAGATCAAACAGATCGTGAATCCACCATATCTGATTTTAAGAGCGATGTGTGTAATTTGTTGATTGCAACTAGTCTTGCTTCTAGAGGGTTAGATGTCAAAGGGGTTGAGCTGGTGATCAACTTTGATACTCCAGATCACTACGAGGACTATGTTCACCGTGTTGGTCGTACAGGCCGAGCTGGCAAGAAAGGTTGTGCTATAACGTTCATCTCTGAGGAAGATGCTAAGTATGCCCCAGATCTTGTCAAAGCATTGGAACTCTCTGAGCAAGCTGTTCCAGATGACCTTAAATCTCTTGCTGATGGTTTCATGGCAAAAATAAAGCAGGGACTTGAGCAGGCCCACGGGACTGGTTACGGGGGCAGTGGTTATAAATTTAATGAAGAGGAAGATGAAGCAAGGAAAGCAGCAAAGAAAGCACAGGCCAAAGAATATGGTTTTGAAGATGACAAGTCAGATTCAGAAGATGAAGATGATGGTGTCAGAAAGGCTGGGGGTGACATTTCACAGCAGGCTGCCCTTGCTCAAATATCAGCTATTGCTGCTGCCTCTATTGGTGGTACGGCTTCAGTTCAGACCCCTGTCTCTACTGCTCAACTGCTTCCTAATGGTGGATTACCTGTTTCTCTTCCCAGTGTCCTTGGTGGTGCAACGTTGCCGGGAACAGCAGCCGTTTTGCCTGGGATGGGTCTGAATCTTGTTGGACATGAAGGGGCAGCTCGAGCAGCAGCAATTGCAGCTGCCATGAATTTGCAGCATAACCTAGCAAAGATTCAAGCTGATGCAATGCCTGAGCACTATGAAGCAGAGTTGGAAATAAATGATATTCCCCAGAATGCTCGATGGAAGGTTACCCACAAGGAAACTTTGGGCCCTATATCAGAGTTGACTGGAGCTGCCATTACTATTAGGGGTCAATATTTCCCACCAGGCAAGGTTCCAGGGCCAGGAGATCGCAAGCTTTACCTGTTTATTGAGGGTCCTACTGAACAATCTGTCAAGAGAGGTAAATCAGAACTAAAACGCAAGTTGGAAGAAATCATGAATCAGTCATTATCACTTCCCAGCGGCGCTCAGCAACGCAGATATCAACTTGAACTACCAAAGTACCAATGGATCATAAGCCAGTGGAAATGTCATGCCAAAGTGGCCGCAGAAGATATCACTGGACTGAAACTTGAATTTCCATGGCAGCAATCTGTTTACCGGCATTCTGAGGAGTGTTCTTTTATACTATTTTTGGCATGTAAGATATCTTCACAATTTTTTGCAGAAACCCGAAAACTACTCAAAAGTGAAAAGTTCAATGTAAAATTCATAAATATGAATGTTTTAAGAAACAGTGTTCTTGTTCATACCGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

1174

Amino Acids

132.1

Weight (kDa)

5.77

Isoelectric Point (pI)

44.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	463 - 634	3.3e-48	DEAD/DEAH box helicase
Helicase_C	PF00271	674 - 780	2.7e-27	Helicase conserved C-terminal domain
KH_12	PF23469	987 - 1070	6.2e-14	KH-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000906)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20920 AT1G20920 AT1G20920 AT1G20920 AT1G20920 AT1G20920 AT1G20920 AT3G09620 AT3G09620
fragaria_vesca	FvH4_3g27090 FvH4_4g11730 FvH4_5g26270 FvH4_5g26270 FvH4_5g26270 FvH4_5g26270 FvH4_5g26270
malus_domestica	MD06G1002800.v1.1 MD09G1086200.v1.1
prunus_persica	Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1 Prupe.5G003900_v2.0.a1
pyrus_communis	pycom06g00290 pycom09g01160
rosa_chinensis	RchiOBHm_Chr5g0049651 RchiOBHm_Chr7g0217001
rosa_laevigata	RLG00000002515 RLG00000034630
rosa_multiflora	Rmu_co8441089.1_g000001 Rmu_sc0001725.1_g000004 Rmu_sc0002114.1_g000002 Rmu_sc0013777.1_g000004 Rmu_sc0013777.1_g000005
rosa_roxburghii	Rroxscaffold_1G00032010 Rroxscaffold_3G00242640
rosa_rugosa	Rorug05G0249600 Rorug05G0249700 Rorug05G0249800 Rorug05G0249900 Rorug05G0250000 Rorug05G0250100 Rorug05G0250200 Rorug07G0164800 Rorug07G0164900 Rorug07G0165000 Rorug07G0165100 Rorug07G0165200 Rorug07G0165300 Rorug07G0165400.1 Rorug07G0165500.1 Rorug07G0165600
rosa_samantha	Rh5AG326000 Rh5BG337300 Rh5CG362000 Rh5DG349500 Rh7AG305000 Rh7BG296500 Rh7CG324100 Rh7DG305100
rosa_wichuraiana	Rw5G030810 Rw7G025910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1547, 1592, 2529
AarI	CACCTGC	1 cut(s)	901
Acc36I	ACCTGC	2 cut(s)	495, 901
AccB1I	GGYRCC	2 cut(s)	1320, 1552
AccBSI	CCGCTC	1 cut(s)	276
AccII	CGCG	1 cut(s)	152
AclI	AACGTT	2 cut(s)	2360, 2810
AclWI	GGATC	5 cut(s)	911, 1082, 1173, 2118, 3281
AcoI	YGGCCR	3 cut(s)	969, 1850, 3306
AcsI	RAATTY	7 cut(s)	937, 1201, 2061, 2531, 2905, 3338, 3470
AcuI	CTGAAG	2 cut(s)	975, 2709
AfaI	GTAC	6 cut(s)	1702, 1732, 2048, 2326, 2719, 3266
AfiI	CCNNNNNNNGG	7 cut(s)	1549, 1637, 1710, 1894, 2768, 2843, 3104
AflIII	ACRYGT	1 cut(s)	2090
AgeI	ACCGGT	1 cut(s)	3515
AhdI	GACNNNNNGTC	1 cut(s)	1614
AhlI	ACTAGT	1 cut(s)	2225
AjnI	CCWGG	8 cut(s)	764, 1039, 1316, 1893, 2836, 3085, 3097, 3103
Alw21I	GWGCWC	1 cut(s)	2955
Alw26I	GTCTC	9 cut(s)	91, 150, 201, 794, 1231, 1422, 1622, 2141, 2746
AlwI	GGATC	5 cut(s)	911, 1082, 1173, 2118, 3281
Ama87I	CYCGRG	1 cut(s)	2877
ApaI	GGGCCC	1 cut(s)	3032
ApoI	RAATTY	7 cut(s)	937, 1201, 2061, 2531, 2905, 3338, 3470
ArsI	GACNNNNNNTTYG	2 cut(s)	1796, 1828
AsiGI	ACCGGT	1 cut(s)	3515
Asp700I	GAANNNNTTC	1 cut(s)	1202
AspA2I	CCTAGG	1 cut(s)	26
AspLEI	GCGC	1 cut(s)	3230
AspS9I	GGNCC	9 cut(s)	811, 1504, 1532, 1998, 2497, 3028, 3029, 3101, 3137
AsuC2I	CCSGG	3 cut(s)	542, 1707, 2817
AsuHPI	GGTGA	8 cut(s)	146, 1013, 1141, 1228, 1883, 2281, 2303, 2666
AvaI	CYCGRG	1 cut(s)	2877
AvaII	GGWCC	4 cut(s)	811, 1504, 1998, 3137
AvrII	CCTAGG	1 cut(s)	26
BaeGI	GKGCMC	1 cut(s)	3032
BaeI	ACNNNNGTAYC	2 cut(s)	3248, 3281
BalI	TGGCCA	1 cut(s)	971
BanI	GGYRCC	2 cut(s)	1320, 1552
BanII	GRGCYC	2 cut(s)	542, 3032
BarI	GAAGNNNNNNTAC	2 cut(s)	3397, 3429
BauI	CACGAG	1 cut(s)	202
BbsI	GAAGAC	2 cut(s)	1104, 1110
Bbv12I	GWGCWC	1 cut(s)	2955
BbvCI	CCTCAGC	2 cut(s)	711, 1490
BceAI	ACGGC	3 cut(s)	839, 2736, 2813
BciT130I	CCWGG	8 cut(s)	766, 1041, 1318, 1895, 2838, 3087, 3099, 3105
BclI	TGATCA	1 cut(s)	2271
BcnI	CCSGG	3 cut(s)	542, 1707, 2817
BcoDI	GTCTC	9 cut(s)	91, 150, 201, 794, 1231, 1422, 1622, 2141, 2746
BcuI	ACTAGT	1 cut(s)	2225
BfaI	CTAG	8 cut(s)	27, 141, 192, 294, 1160, 2226, 2238, 2921
BfmI	CTRYAG	2 cut(s)	978, 1757
BfoI	RGCGCY	1 cut(s)	3231
BfuAI	ACCTGC	2 cut(s)	495, 901
BglI	GCCNNNNNGGC	2 cut(s)	2337, 3305
BglII	AGATCT	1 cut(s)	2395
BlnI	CCTAGG	1 cut(s)	26
BlpI	GCTNAGC	1 cut(s)	3231
Bme18I	GGWCC	4 cut(s)	811, 1504, 1998, 3137
BmeRI	GACNNNNNGTC	1 cut(s)	1614
BmeT110I	CYCGRG	1 cut(s)	2877
BmgT120I	GGNCC	9 cut(s)	811, 1504, 1532, 1998, 2497, 3028, 3029, 3101, 3137
BmiI	GGNNCC	6 cut(s)	853, 1322, 1554, 3030, 3096, 3138
BmrI	ACTGGG	2 cut(s)	1622, 2784
BmsI	GCATC	4 cut(s)	265, 916, 2368, 2929
BmuI	ACTGGG	2 cut(s)	1622, 2784
BoxI	GACNNNNGTC	2 cut(s)	1142, 2738
BpiI	GAAGAC	2 cut(s)	1104, 1110
BpmI	CTGGAG	3 cut(s)	1545, 2271, 3069
Bpu10I	CCTNAGC	7 cut(s)	711, 1398, 1456, 1490, 2105, 2117, 2949
Bpu1102I	GCTNAGC	1 cut(s)	3231
BpuEI	CTTGAG	2 cut(s)	794, 2510
BpuMI	CCSGG	3 cut(s)	542, 1707, 2817
BsaAI	YACGTR	1 cut(s)	1913
BsaBI	GATNNNNATC	1 cut(s)	1086
BsaI	GGTCTC	2 cut(s)	1231, 1622
BsaJI	CCNNGG	8 cut(s)	26, 1040, 1705, 2500, 2797, 2837, 3098, 3344
BsaWI	WCCGGW	1 cut(s)	3515
Bsc4I	CCNNNNNNNGG	7 cut(s)	1549, 1637, 1710, 1894, 2768, 2843, 3104
Bse118I	RCCGGY	2 cut(s)	3363, 3515
Bse1I	ACTGG	9 cut(s)	989, 1333, 1456, 1617, 2512, 2790, 3052, 3283, 3328
Bse3DI	GCAATG	2 cut(s)	1440, 2949
Bse8I	GATNNNNATC	1 cut(s)	1086
BseBI	CCWGG	8 cut(s)	766, 1041, 1318, 1895, 2838, 3087, 3099, 3105
BseDI	CCNNGG	8 cut(s)	26, 1040, 1705, 2500, 2797, 2837, 3098, 3344
BseJI	GATNNNNATC	1 cut(s)	1086
BseLI	CCNNNNNNNGG	7 cut(s)	1549, 1637, 1710, 1894, 2768, 2843, 3104
BseMI	GCAATG	2 cut(s)	1440, 2949
BseNI	ACTGG	9 cut(s)	989, 1333, 1456, 1617, 2512, 2790, 3052, 3283, 3328
BseSI	GKGCMC	1 cut(s)	3032
BseYI	CCCAGC	2 cut(s)	2648, 3221
BsgI	GTGCAG	1 cut(s)	873
Bsh1236I	CGCG	1 cut(s)	152
BshNI	GGYRCC	2 cut(s)	1320, 1552
BshTI	ACCGGT	1 cut(s)	3515
BsiHKAI	GWGCWC	1 cut(s)	2955
BsiHKCI	CYCGRG	1 cut(s)	2877
BsiSI	CCGG	5 cut(s)	542, 1707, 2816, 3364, 3516
BslFI	GGGAC	6 cut(s)	380, 595, 1082, 1283, 2499, 2518
BslI	CCNNNNNNNGG	7 cut(s)	1549, 1637, 1710, 1894, 2768, 2843, 3104
BsmAI	GTCTC	9 cut(s)	91, 150, 201, 794, 1231, 1422, 1622, 2141, 2746
BsmFI	GGGAC	6 cut(s)	380, 595, 1082, 1283, 2499, 2518
BsmI	GAATGC	3 cut(s)	478, 2998, 3367
Bso31I	GGTCTC	2 cut(s)	1231, 1622
BsoBI	CYCGRG	1 cut(s)	2877
Bsp120I	GGGCCC	1 cut(s)	3028
Bsp1286I	GDGCHC	3 cut(s)	542, 2955, 3032
Bsp1407I	TGTACA	1 cut(s)	1730
Bsp1720I	GCTNAGC	1 cut(s)	3231
Bsp19I	CCATGG	1 cut(s)	3344
BspFNI	CGCG	1 cut(s)	152
BspHI	TCATGA	1 cut(s)	3198
BspLI	GGNNCC	6 cut(s)	853, 1322, 1554, 3030, 3096, 3138
BspMAI	CTGCAG	2 cut(s)	982, 1761
BspMI	ACCTGC	2 cut(s)	495, 901
BspPI	GGATC	5 cut(s)	911, 1082, 1173, 2118, 3281
BspT107I	GGYRCC	2 cut(s)	1320, 1552
BspTNI	GGTCTC	2 cut(s)	1231, 1622
BsrBI	CCGCTC	1 cut(s)	276
BsrDI	GCAATG	2 cut(s)	1440, 2949
BsrFI	RCCGGY	2 cut(s)	3363, 3515
BsrGI	TGTACA	1 cut(s)	1730
BsrI	ACTGG	9 cut(s)	989, 1333, 1456, 1617, 2512, 2790, 3052, 3283, 3328
BssAI	RCCGGY	2 cut(s)	3363, 3515
BssECI	CCNNGG	8 cut(s)	26, 1040, 1705, 2500, 2797, 2837, 3098, 3344
BssSI	CACGAG	1 cut(s)	202
BssT1I	CCWWGG	3 cut(s)	26, 2797, 3344
Bst2BI	CACGAG	1 cut(s)	202
Bst2UI	CCWGG	8 cut(s)	766, 1041, 1318, 1895, 2838, 3087, 3099, 3105
Bst4CI	ACNGT	5 cut(s)	946, 1586, 2101, 2315, 3500
Bst6I	CTCTTC	5 cut(s)	405, 545, 792, 2535, 2790
BstAPI	GCANNNNNTGC	1 cut(s)	2891
BstAUI	TGTACA	1 cut(s)	1730
BstBAI	YACGTR	1 cut(s)	1913
BstC8I	GCNNGC	6 cut(s)	969, 2338, 2427, 2496, 2670, 3116
BstDSI	CCRYGG	2 cut(s)	2500, 3344
BstEII	GGTNACC	1 cut(s)	3007
BstFNI	CGCG	1 cut(s)	152
BstH2I	RGCGCY	1 cut(s)	3231
BstHHI	GCGC	1 cut(s)	3230
BstMAI	GTCTC	9 cut(s)	91, 150, 201, 794, 1231, 1422, 1622, 2141, 2746
BstNI	CCWGG	8 cut(s)	766, 1041, 1318, 1895, 2838, 3087, 3099, 3105
BstNSI	RCATGY	3 cut(s)	728, 2087, 3405
BstPAI	GACNNNNGTC	2 cut(s)	1142, 2738
BstPI	GGTNACC	1 cut(s)	3007
BstSFI	CTRYAG	2 cut(s)	978, 1757
BstSLI	GKGCMC	1 cut(s)	3032
BstUI	CGCG	1 cut(s)	152
BstV2I	GAAGAC	2 cut(s)	1104, 1110
BstX2I	RGATCY	2 cut(s)	2110, 2395
BstXI	CCANNNNNNTGG	3 cut(s)	1612, 1664, 2507
BstYI	RGATCY	2 cut(s)	2110, 2395
BtgI	CCRYGG	2 cut(s)	2500, 3344
BtgZI	GCGATG	1 cut(s)	2214
BtsI	GCAGTG	2 cut(s)	1410, 2527
BveI	ACCTGC	2 cut(s)	495, 901
Cac8I	GCNNGC	6 cut(s)	969, 2338, 2427, 2496, 2670, 3116
CciI	TCATGA	1 cut(s)	3198
CfoI	GCGC	1 cut(s)	3230
Cfr10I	RCCGGY	2 cut(s)	3363, 3515
Cfr13I	GGNCC	9 cut(s)	811, 1504, 1532, 1998, 2497, 3028, 3029, 3101, 3137
CsiI	ACCWGGT	1 cut(s)	1893
Csp6I	GTAC	6 cut(s)	1701, 1731, 2047, 2325, 2718, 3265
CspAI	ACCGGT	1 cut(s)	3515
CviQI	GTAC	6 cut(s)	1701, 1731, 2047, 2325, 2718, 3265
DriI	GACNNNNNGTC	1 cut(s)	1614
EaeI	YGGCCR	3 cut(s)	969, 1850, 3306
Eam1104I	CTCTTC	5 cut(s)	405, 545, 792, 2535, 2790
Eam1105I	GACNNNNNGTC	1 cut(s)	1614
EarI	CTCTTC	5 cut(s)	405, 545, 792, 2535, 2790
Eco130I	CCWWGG	3 cut(s)	26, 2797, 3344
Eco24I	GRGCYC	2 cut(s)	542, 3032
Eco31I	GGTCTC	2 cut(s)	1231, 1622
Eco32I	GATATC	4 cut(s)	2129, 3245, 3319, 3411
Eco47I	GGWCC	4 cut(s)	811, 1504, 1998, 3137
Eco57I	CTGAAG	2 cut(s)	975, 2709
Eco88I	CYCGRG	1 cut(s)	2877
Eco91I	GGTNACC	1 cut(s)	3007
EcoO109I	RGGNCCY	2 cut(s)	3029, 3137
EcoO65I	GGTNACC	1 cut(s)	3007
EcoRI	GAATTC	1 cut(s)	937
EcoRII	CCWGG	8 cut(s)	764, 1039, 1316, 1893, 2836, 3085, 3097, 3103
EcoRV	GATATC	4 cut(s)	2129, 3245, 3319, 3411
EcoT14I	CCWWGG	3 cut(s)	26, 2797, 3344
EcoT38I	GRGCYC	2 cut(s)	542, 3032
ErhI	CCWWGG	3 cut(s)	26, 2797, 3344
FaqI	GGGAC	6 cut(s)	380, 595, 1082, 1283, 2499, 2518
FauI	CCCGC	1 cut(s)	1671
FauNDI	CATATG	1 cut(s)	1474
FbaI	TGATCA	1 cut(s)	2271
FriOI	GRGCYC	2 cut(s)	542, 3032
FspBI	CTAG	8 cut(s)	27, 141, 192, 294, 1160, 2226, 2238, 2921
GlaI	GCGC	1 cut(s)	3229
GsaI	CCCAGC	2 cut(s)	2652, 3225
GsuI	CTGGAG	3 cut(s)	1545, 2271, 3069
HaeII	RGCGCY	1 cut(s)	3231
HapII	CCGG	5 cut(s)	542, 1707, 2816, 3364, 3516
HhaI	GCGC	1 cut(s)	3230
Hin6I	GCGC	1 cut(s)	3228
HinP1I	GCGC	1 cut(s)	3228
HincII	GTYRAC	1 cut(s)	3045
HindII	GTYRAC	1 cut(s)	3045
HindIII	AAGCTT	2 cut(s)	1364, 3116
HpaII	CCGG	5 cut(s)	542, 1707, 2816, 3364, 3516
HphI	GGTGA	8 cut(s)	146, 1013, 1141, 1228, 1883, 2281, 2303, 2666
Hpy166II	GTNNAC	6 cut(s)	1119, 1424, 1966, 2311, 3045, 3361
Hpy188III	TCNNGA	9 cut(s)	65, 674, 1195, 2171, 2238, 2288, 2435, 3157, 3199
Hpy8I	GTNNAC	6 cut(s)	1119, 1424, 1966, 2311, 3045, 3361
HpyAV	CCTTC	7 cut(s)	25, 352, 378, 725, 1213, 2861, 2998
HpyCH4III	ACNGT	5 cut(s)	946, 1586, 2101, 2315, 3500
HpyCH4IV	ACGT	5 cut(s)	1883, 1912, 2090, 2360, 2810
HpySE526I	ACGT	5 cut(s)	1883, 1912, 2090, 2360, 2810
HspAI	GCGC	1 cut(s)	3228
Ksp22I	TGATCA	1 cut(s)	2271
LmnI	GCTCC	4 cut(s)	34, 851, 1564, 3050
LweI	GCATC	4 cut(s)	265, 916, 2368, 2929
MabI	ACCWGGT	1 cut(s)	1893
MaeI	CTAG	8 cut(s)	27, 141, 192, 294, 1160, 2226, 2238, 2921
MaeII	ACGT	5 cut(s)	1883, 1912, 2090, 2360, 2810
MaeIII	GTNAC	7 cut(s)	497, 1129, 1765, 2093, 2510, 2654, 3007
MbiI	CCGCTC	1 cut(s)	276
MfeI	CAATTG	3 cut(s)	1268, 1689, 2889
MflI	RGATCY	2 cut(s)	2110, 2395
MhlI	GDGCHC	3 cut(s)	542, 2955, 3032
MlsI	TGGCCA	1 cut(s)	971
MluNI	TGGCCA	1 cut(s)	971
MlyI	GAGTC	1 cut(s)	1858
MmeI	TCCRAC	3 cut(s)	2839, 2949, 3168
Mox20I	TGGCCA	1 cut(s)	971
MroXI	GAANNNNTTC	1 cut(s)	1202
MscI	TGGCCA	1 cut(s)	971
MseI	TTAA	8 cut(s)	123, 689, 1311, 1922, 2193, 2445, 2535, 3491
MslI	CAYNNNNRTG	4 cut(s)	66, 522, 1214, 3479
Msp20I	TGGCCA	1 cut(s)	971
MspA1I	CMGCKG	3 cut(s)	887, 2897, 3225
MspI	CCGG	5 cut(s)	542, 1707, 2816, 3364, 3516
MunI	CAATTG	3 cut(s)	1268, 1689, 2889
Mva1269I	GAATGC	3 cut(s)	478, 2998, 3367
MvaI	CCWGG	8 cut(s)	766, 1041, 1318, 1895, 2838, 3087, 3099, 3105
MvnI	CGCG	1 cut(s)	152
NciI	CCSGG	3 cut(s)	542, 1707, 2817
NcoI	CCATGG	1 cut(s)	3344
NdeI	CATATG	1 cut(s)	1474
NlaIV	GGNNCC	6 cut(s)	853, 1322, 1554, 3030, 3096, 3138
NmeAIII	GCCGAG	1 cut(s)	2357
NmuCI	GTSAC	3 cut(s)	1129, 2093, 2654
NspI	RCATGY	3 cut(s)	728, 2087, 3405
PaeR7I	CTCGAG	1 cut(s)	2877
PagI	TCATGA	1 cut(s)	3198
PaqCI	CACCTGC	1 cut(s)	901
PcsI	WCGNNNNNNNCGW	1 cut(s)	2329
PctI	GAATGC	3 cut(s)	478, 2998, 3367
PdmI	GAANNNNTTC	1 cut(s)	1202
PinAI	ACCGGT	1 cut(s)	3515
PleI	GAGTC	1 cut(s)	1858
PpsI	GAGTC	1 cut(s)	1858
Ppu21I	YACGTR	1 cut(s)	1913
PpuMI	RGGWCCY	1 cut(s)	3137
PshAI	GACNNNNGTC	2 cut(s)	1142, 2738
PsiI	TTATAA	3 cut(s)	1547, 1592, 2529
Psp1406I	AACGTT	2 cut(s)	2360, 2810
Psp5II	RGGWCCY	1 cut(s)	3137
Psp6I	CCWGG	8 cut(s)	764, 1039, 1316, 1893, 2836, 3085, 3097, 3103
PspEI	GGTNACC	1 cut(s)	3007
PspFI	CCCAGC	2 cut(s)	2648, 3221
PspGI	CCWGG	8 cut(s)	764, 1039, 1316, 1893, 2836, 3085, 3097, 3103
PspN4I	GGNNCC	6 cut(s)	853, 1322, 1554, 3030, 3096, 3138
PspOMI	GGGCCC	1 cut(s)	3028
PspPI	GGNCC	9 cut(s)	811, 1504, 1532, 1998, 2497, 3028, 3029, 3101, 3137
PspPPI	RGGWCCY	1 cut(s)	3137
PspXI	VCTCGAGB	1 cut(s)	2877
PstI	CTGCAG	2 cut(s)	982, 1761
PsuI	RGATCY	2 cut(s)	2110, 2395
PvuII	CAGCTG	2 cut(s)	887, 2897
RsaI	GTAC	6 cut(s)	1702, 1732, 2048, 2326, 2719, 3266
RsaNI	GTAC	6 cut(s)	1701, 1731, 2047, 2325, 2718, 3265
RseI	CAYNNNNRTG	4 cut(s)	66, 522, 1214, 3479
SaqAI	TTAA	8 cut(s)	123, 689, 1311, 1922, 2193, 2445, 2535, 3491
Sau96I	GGNCC	9 cut(s)	811, 1504, 1532, 1998, 2497, 3028, 3029, 3101, 3137
SchI	GAGTC	1 cut(s)	1858
SduI	GDGCHC	3 cut(s)	542, 2955, 3032
SexAI	ACCWGGT	1 cut(s)	1893
SfaNI	GCATC	4 cut(s)	265, 916, 2368, 2929
SfcI	CTRYAG	2 cut(s)	978, 1757
Sfr274I	CTCGAG	1 cut(s)	2877
SinI	GGWCC	4 cut(s)	811, 1504, 1998, 3137
SlaI	CTCGAG	1 cut(s)	2877
SmiMI	CAYNNNNRTG	4 cut(s)	66, 522, 1214, 3479
SmlI	CTYRAG	3 cut(s)	773, 2489, 2877
SmoI	CTYRAG	3 cut(s)	773, 2489, 2877
SpeI	ACTAGT	1 cut(s)	2225
SspI	AATATT	2 cut(s)	1846, 3077
SspMI	CTAG	8 cut(s)	27, 141, 192, 294, 1160, 2226, 2238, 2921
StyI	CCWWGG	3 cut(s)	26, 2797, 3344
TaaI	ACNGT	5 cut(s)	946, 1586, 2101, 2315, 3500
TaiI	ACGT	5 cut(s)	1886, 1915, 2093, 2363, 2813
TatI	WGTACW	1 cut(s)	1730
TauI	GCSGC	4 cut(s)	256, 451, 3228, 3311
Tru1I	TTAA	8 cut(s)	123, 689, 1311, 1922, 2193, 2445, 2535, 3491
Tru9I	TTAA	8 cut(s)	123, 689, 1311, 1922, 2193, 2445, 2535, 3491
TseFI	GTSAC	3 cut(s)	1129, 2093, 2654
Tsp45I	GTSAC	3 cut(s)	1129, 2093, 2654
TspGWI	ACGGA	1 cut(s)	834
VpaK11BI	GGWCC	4 cut(s)	811, 1504, 1998, 3137
XapI	RAATTY	7 cut(s)	937, 1201, 2061, 2531, 2905, 3338, 3470
XbaI	TCTAGA	1 cut(s)	2237
XceI	RCATGY	3 cut(s)	728, 2087, 3405
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3267
XhoI	CTCGAG	1 cut(s)	2877
XmaJI	CCTAGG	1 cut(s)	26
XmnI	GAANNNNTTC	1 cut(s)	1202
XspI	CTAG	8 cut(s)	27, 141, 192, 294, 1160, 2226, 2238, 2921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.