DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 94 |
| Acc36I | ACCTGC | 2 cut(s) | 1571, 2458 |
| AccB1I | GGYRCC | 2 cut(s) | 1893, 2125 |
| AccBSI | CCGCTC | 2 cut(s) | 787, 3137 |
| AccI | GTMKAC | 1 cut(s) | 2208 |
| AccII | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| AccIII | TCCGGA | 1 cut(s) | 232 |
| AclI | AACGTT | 1 cut(s) | 3593 |
| AclWI | GGATC | 6 cut(s) | 519, 525, 1439, 1655, 2035, 3493 |
| AcoI | YGGCCR | 2 cut(s) | 2902, 2911 |
| AcsI | RAATTY | 5 cut(s) | 321, 1773, 2634, 2848, 3104 |
| AcuI | CTGAAG | 3 cut(s) | 207, 389, 2267 |
| AcyI | GRCGYC | 1 cut(s) | 3363 |
| AfaI | GTAC | 6 cut(s) | 629, 1001, 2052, 2275, 2649, 2899 |
| AflII | CTTAAG | 1 cut(s) | 2690 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 1267, 3347 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 383, 415, 2502, 2534 |
| Alw26I | GTCTC | 5 cut(s) | 1322, 1995, 2195, 2714, 2944 |
| AlwI | GGATC | 6 cut(s) | 519, 525, 1439, 1655, 2035, 3493 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 185, 1424, 1978 |
| Ama87I | CYCGRG | 1 cut(s) | 3447 |
| Aor13HI | TCCGGA | 1 cut(s) | 232 |
| AoxI | GGCC | 6 cut(s) | 2105, 2724, 2902, 2911, 3598, 3706 |
| ApaI | GGGCCC | 2 cut(s) | 3602, 3710 |
| ApoI | RAATTY | 5 cut(s) | 321, 1773, 2634, 2848, 3104 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 2369, 2401, 2407, 2439, 3708, 3740 |
| AseI | ATTAAT | 1 cut(s) | 1410 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 3210 |
| AspLEI | GCGC | 4 cut(s) | 185, 218, 361, 377 |
| AspS9I | GGNCC | 8 cut(s) | 1642, 2077, 2105, 2724, 3598, 3599, 3706, 3707 |
| AsuII | TTCGAA | 1 cut(s) | 3214 |
| AvaI | CYCGRG | 1 cut(s) | 3447 |
| AvaII | GGWCC | 2 cut(s) | 1642, 2077 |
| BaeGI | GKGCMC | 2 cut(s) | 3602, 3710 |
| BanI | GGYRCC | 2 cut(s) | 1893, 2125 |
| BanII | GRGCYC | 3 cut(s) | 1070, 3602, 3710 |
| BbsI | GAAGAC | 1 cut(s) | 3183 |
| BbvCI | CCTCAGC | 2 cut(s) | 1574, 2063 |
| BccI | CCATC | 8 cut(s) | 129, 656, 1049, 1286, 1403, 2096, 3431, 3564 |
| BceAI | ACGGC | 1 cut(s) | 2889 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 34, 68 |
| BclI | TGATCA | 1 cut(s) | 244 |
| BcoDI | GTCTC | 5 cut(s) | 1322, 1995, 2195, 2714, 2944 |
| BfaI | CTAG | 6 cut(s) | 428, 471, 2811, 2819, 2955, 3567 |
| BfmI | CTRYAG | 3 cut(s) | 1425, 3291, 3453 |
| BfoI | RGCGCY | 3 cut(s) | 186, 362, 378 |
| BfrI | CTTAAG | 1 cut(s) | 2690 |
| BfuAI | ACCTGC | 2 cut(s) | 1571, 2458 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 2113, 2910, 2919 |
| BglII | AGATCT | 4 cut(s) | 1797, 2683, 2968, 3765 |
| Bme18I | GGWCC | 2 cut(s) | 1642, 2077 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 1267, 3347 |
| BmeT110I | CYCGRG | 1 cut(s) | 3447 |
| BmgT120I | GGNCC | 8 cut(s) | 1642, 2077, 2105, 2724, 3598, 3599, 3706, 3707 |
| BmiI | GGNNCC | 8 cut(s) | 287, 1643, 1895, 2127, 2725, 3600, 3666, 3708 |
| BmrI | ACTGGG | 1 cut(s) | 101 |
| BmsI | GCATC | 8 cut(s) | 24, 32, 1381, 1467, 1800, 2659, 2941, 3499 |
| BmuI | ACTGGG | 1 cut(s) | 101 |
| BpiI | GAAGAC | 1 cut(s) | 3183 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 364, 396 |
| BpmI | CTGGAG | 3 cut(s) | 2949, 3639, 3813 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1574, 1971, 2063 |
| Bpu14I | TTCGAA | 1 cut(s) | 3214 |
| BpuEI | CTTGAG | 3 cut(s) | 1667, 1786, 2611 |
| BsaAI | YACGTR | 1 cut(s) | 631 |
| BsaBI | GATNNNNATC | 3 cut(s) | 510, 516, 1659 |
| BsaHI | GRCGYC | 1 cut(s) | 3363 |
| BsaI | GGTCTC | 1 cut(s) | 2195 |
| BsaWI | WCCGGW | 2 cut(s) | 232, 1833 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1015, 1045, 1371, 1401 |
| Bse3DI | GCAATG | 5 cut(s) | 229, 453, 2779, 3303, 3519 |
| Bse8I | GATNNNNATC | 3 cut(s) | 510, 516, 1659 |
| BseAI | TCCGGA | 1 cut(s) | 232 |
| BseJI | GATNNNNATC | 3 cut(s) | 510, 516, 1659 |
| BseMI | GCAATG | 5 cut(s) | 229, 453, 2779, 3303, 3519 |
| BseRI | GAGGAG | 9 cut(s) | 251, 508, 579, 635, 910, 1139, 1142, 1192, 1195 |
| BseSI | GKGCMC | 2 cut(s) | 3602, 3710 |
| BseYI | CCCAGC | 1 cut(s) | 2096 |
| Bsh1236I | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| Bsh1285I | CGRYCG | 1 cut(s) | 131 |
| BshFI | GGCC | 6 cut(s) | 2107, 2726, 2904, 2913, 3600, 3708 |
| BshNI | GGYRCC | 2 cut(s) | 1893, 2125 |
| BsiEI | CGRYCG | 1 cut(s) | 131 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3447 |
| BsiSI | CCGG | 3 cut(s) | 233, 1834, 2914 |
| BslFI | GGGAC | 6 cut(s) | 746, 1655, 2843, 3072, 3091, 3424 |
| BsmAI | GTCTC | 5 cut(s) | 1322, 1995, 2195, 2714, 2944 |
| BsmFI | GGGAC | 6 cut(s) | 746, 1655, 2843, 3072, 3091, 3424 |
| BsmI | GAATGC | 2 cut(s) | 1504, 3568 |
| BsnI | GGCC | 6 cut(s) | 2107, 2726, 2904, 2913, 3600, 3708 |
| Bso31I | GGTCTC | 1 cut(s) | 2195 |
| BsoBI | CYCGRG | 1 cut(s) | 3447 |
| Bsp119I | TTCGAA | 1 cut(s) | 3214 |
| Bsp120I | GGGCCC | 2 cut(s) | 3598, 3706 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1070, 3602, 3710 |
| Bsp13I | TCCGGA | 1 cut(s) | 232 |
| Bsp1407I | TGTACA | 1 cut(s) | 2647 |
| Bsp19I | CCATGG | 1 cut(s) | 3073 |
| Bsp68I | TCGCGA | 1 cut(s) | 133 |
| BspANI | GGCC | 6 cut(s) | 2107, 2726, 2904, 2913, 3600, 3708 |
| BspEI | TCCGGA | 1 cut(s) | 232 |
| BspFNI | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| BspLI | GGNNCC | 8 cut(s) | 287, 1643, 1895, 2127, 2725, 3600, 3666, 3708 |
| BspMAI | CTGCAG | 2 cut(s) | 1429, 3457 |
| BspMI | ACCTGC | 2 cut(s) | 1571, 2458 |
| BspPI | GGATC | 6 cut(s) | 519, 525, 1439, 1655, 2035, 3493 |
| BspQI | GCTCTTC | 2 cut(s) | 365, 3360 |
| BspT104I | TTCGAA | 1 cut(s) | 3214 |
| BspT107I | GGYRCC | 2 cut(s) | 1893, 2125 |
| BspTI | CTTAAG | 1 cut(s) | 2690 |
| BspTNI | GGTCTC | 1 cut(s) | 2195 |
| BsrBI | CCGCTC | 2 cut(s) | 787, 3137 |
| BsrDI | GCAATG | 5 cut(s) | 229, 453, 2779, 3303, 3519 |
| BsrGI | TGTACA | 1 cut(s) | 2647 |
| BssNAI | GTATAC | 1 cut(s) | 2209 |
| BssNI | GRCGYC | 1 cut(s) | 3363 |
| BssT1I | CCWWGG | 2 cut(s) | 2178, 3073 |
| Bst1107I | GTATAC | 1 cut(s) | 2209 |
| Bst4CI | ACNGT | 7 cut(s) | 1084, 1507, 2159, 2440, 2888, 3338, 3720 |
| Bst6I | CTCTTC | 8 cut(s) | 365, 447, 773, 840, 857, 980, 3360, 3756 |
| BstACI | GRCGYC | 1 cut(s) | 3363 |
| BstAFI | CTTAAG | 1 cut(s) | 2690 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 224, 3461 |
| BstAUI | TGTACA | 1 cut(s) | 2647 |
| BstBAI | YACGTR | 1 cut(s) | 631 |
| BstBI | TTCGAA | 1 cut(s) | 3214 |
| BstDSI | CCRYGG | 2 cut(s) | 289, 3073 |
| BstFNI | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| BstH2I | RGCGCY | 3 cut(s) | 186, 362, 378 |
| BstHHI | GCGC | 4 cut(s) | 185, 218, 361, 377 |
| BstMAI | GTCTC | 5 cut(s) | 1322, 1995, 2195, 2714, 2944 |
| BstMCI | CGRYCG | 1 cut(s) | 131 |
| BstSFI | CTRYAG | 3 cut(s) | 1425, 3291, 3453 |
| BstSLI | GKGCMC | 2 cut(s) | 3602, 3710 |
| BstUI | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| BstV2I | GAAGAC | 1 cut(s) | 3183 |
| BstX2I | RGATCY | 5 cut(s) | 1797, 2683, 2968, 3498, 3765 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2185, 3080 |
| BstYI | RGATCY | 5 cut(s) | 1797, 2683, 2968, 3498, 3765 |
| BstZ17I | GTATAC | 1 cut(s) | 2209 |
| BsuRI | GGCC | 6 cut(s) | 2107, 2726, 2904, 2913, 3600, 3708 |
| BtgI | CCRYGG | 2 cut(s) | 289, 3073 |
| BtgZI | GCGATG | 2 cut(s) | 55, 148 |
| BtsI | GCAGTG | 3 cut(s) | 201, 1976, 3439 |
| BtsIMutI | CAGTG | 8 cut(s) | 28, 89, 114, 201, 1976, 2164, 2806, 3439 |
| BtuMI | TCGCGA | 1 cut(s) | 133 |
| BveI | ACCTGC | 2 cut(s) | 1571, 2458 |
| CaiI | CAGNNNCTG | 3 cut(s) | 185, 1424, 1978 |
| CfoI | GCGC | 4 cut(s) | 185, 218, 361, 377 |
| Cfr13I | GGNCC | 8 cut(s) | 1642, 2077, 2105, 2724, 3598, 3599, 3706, 3707 |
| CseI | GACGC | 1 cut(s) | 3352 |
| Csp6I | GTAC | 6 cut(s) | 628, 1000, 2051, 2274, 2648, 2898 |
| CviQI | GTAC | 6 cut(s) | 628, 1000, 2051, 2274, 2648, 2898 |
| DraI | TTTAAA | 1 cut(s) | 3103 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 94 |
| DriI | GACNNNNNGTC | 2 cut(s) | 1267, 3347 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 94 |
| EaeI | YGGCCR | 2 cut(s) | 2902, 2911 |
| Eam1104I | CTCTTC | 8 cut(s) | 365, 447, 773, 840, 857, 980, 3360, 3756 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 1267, 3347 |
| EarI | CTCTTC | 8 cut(s) | 365, 447, 773, 840, 857, 980, 3360, 3756 |
| EciI | GGCGGA | 1 cut(s) | 2538 |
| Eco130I | CCWWGG | 2 cut(s) | 2178, 3073 |
| Eco24I | GRGCYC | 3 cut(s) | 1070, 3602, 3710 |
| Eco31I | GGTCTC | 1 cut(s) | 2195 |
| Eco32I | GATATC | 4 cut(s) | 2164, 2702, 3232, 3815 |
| Eco47I | GGWCC | 2 cut(s) | 1642, 2077 |
| Eco57I | CTGAAG | 3 cut(s) | 207, 389, 2267 |
| Eco88I | CYCGRG | 1 cut(s) | 3447 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1642, 3599, 3706, 3707 |
| EcoRI | GAATTC | 1 cut(s) | 321 |
| EcoRV | GATATC | 4 cut(s) | 2164, 2702, 3232, 3815 |
| EcoT14I | CCWWGG | 2 cut(s) | 2178, 3073 |
| EcoT38I | GRGCYC | 3 cut(s) | 1070, 3602, 3710 |
| ErhI | CCWWGG | 2 cut(s) | 2178, 3073 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 159, 191, 310, 342, 3677, 3709 |
| FaqI | GGGAC | 6 cut(s) | 746, 1655, 2843, 3072, 3091, 3424 |
| FauI | CCCGC | 3 cut(s) | 22, 65, 2244 |
| FbaI | TGATCA | 1 cut(s) | 244 |
| FblI | GTMKAC | 1 cut(s) | 2208 |
| FriOI | GRGCYC | 3 cut(s) | 1070, 3602, 3710 |
| FspBI | CTAG | 6 cut(s) | 428, 471, 2811, 2819, 2955, 3567 |
| GlaI | GCGC | 4 cut(s) | 184, 217, 360, 376 |
| GsaI | CCCAGC | 1 cut(s) | 2100 |
| GsuI | CTGGAG | 3 cut(s) | 2949, 3639, 3813 |
| HaeII | RGCGCY | 3 cut(s) | 186, 362, 378 |
| HaeIII | GGCC | 6 cut(s) | 2107, 2726, 2904, 2913, 3600, 3708 |
| HapII | CCGG | 3 cut(s) | 233, 1834, 2914 |
| HgaI | GACGC | 1 cut(s) | 3352 |
| HhaI | GCGC | 4 cut(s) | 185, 218, 361, 377 |
| Hin1I | GRCGYC | 1 cut(s) | 3363 |
| Hin6I | GCGC | 4 cut(s) | 183, 216, 359, 375 |
| HinP1I | GCGC | 4 cut(s) | 183, 216, 359, 375 |
| HincII | GTYRAC | 1 cut(s) | 1738 |
| HindII | GTYRAC | 1 cut(s) | 1738 |
| HindIII | AAGCTT | 1 cut(s) | 1455 |
| HpaII | CCGG | 3 cut(s) | 233, 1834, 2914 |
| Hpy166II | GTNNAC | 7 cut(s) | 1738, 2209, 2243, 2751, 2884, 3341, 3615 |
| Hpy8I | GTNNAC | 7 cut(s) | 1738, 2209, 2243, 2751, 2884, 3341, 3615 |
| Hpy99I | CGWCG | 4 cut(s) | 318, 351, 591, 3368 |
| HpyCH4III | ACNGT | 7 cut(s) | 1084, 1507, 2159, 2440, 2888, 3338, 3720 |
| HpyCH4IV | ACGT | 5 cut(s) | 630, 829, 1341, 2896, 3593 |
| HpySE526I | ACGT | 5 cut(s) | 630, 829, 1341, 2896, 3593 |
| Hsp92I | GRCGYC | 1 cut(s) | 3363 |
| HspAI | GCGC | 4 cut(s) | 183, 216, 359, 375 |
| Kpn2I | TCCGGA | 1 cut(s) | 232 |
| Ksp22I | TGATCA | 1 cut(s) | 244 |
| LguI | GCTCTTC | 2 cut(s) | 365, 3360 |
| LmnI | GCTCC | 8 cut(s) | 238, 291, 784, 961, 2137, 2830, 2968, 3620 |
| LweI | GCATC | 8 cut(s) | 24, 32, 1381, 1467, 1800, 2659, 2941, 3499 |
| MaeI | CTAG | 6 cut(s) | 428, 471, 2811, 2819, 2955, 3567 |
| MaeII | ACGT | 5 cut(s) | 630, 829, 1341, 2896, 3593 |
| MaeIII | GTNAC | 7 cut(s) | 60, 85, 112, 578, 2338, 2604, 3577 |
| MbiI | CCGCTC | 2 cut(s) | 787, 3137 |
| MfeI | CAATTG | 3 cut(s) | 1518, 1841, 2555 |
| MflI | RGATCY | 5 cut(s) | 1797, 2683, 2968, 3498, 3765 |
| MhlI | GDGCHC | 3 cut(s) | 1070, 3602, 3710 |
| MlyI | GAGTC | 8 cut(s) | 131, 505, 583, 771, 1279, 1622, 2756, 3298 |
| MmeI | TCCRAC | 3 cut(s) | 1996, 2872, 3519 |
| MroI | TCCGGA | 1 cut(s) | 232 |
| MroXI | GAANNNNTTC | 1 cut(s) | 3210 |
| MseI | TTAA | 7 cut(s) | 1205, 1410, 2691, 2766, 3102, 3108, 3746 |
| MslI | CAYNNNNRTG | 4 cut(s) | 924, 1050, 1988, 3507 |
| MspA1I | CMGCKG | 1 cut(s) | 1421 |
| MspCI | CTTAAG | 1 cut(s) | 2690 |
| MspI | CCGG | 3 cut(s) | 233, 1834, 2914 |
| MunI | CAATTG | 3 cut(s) | 1518, 1841, 2555 |
| Mva1269I | GAATGC | 2 cut(s) | 1504, 3568 |
| MvnI | CGCG | 5 cut(s) | 8, 29, 40, 127, 133 |
| NcoI | CCATGG | 1 cut(s) | 3073 |
| NlaIV | GGNNCC | 8 cut(s) | 287, 1643, 1895, 2127, 2725, 3600, 3666, 3708 |
| NmeAIII | GCCGAG | 1 cut(s) | 2443 |
| NmuCI | GTSAC | 5 cut(s) | 60, 85, 112, 578, 3577 |
| NruI | TCGCGA | 1 cut(s) | 133 |
| NspV | TTCGAA | 1 cut(s) | 3214 |
| PciSI | GCTCTTC | 2 cut(s) | 365, 3360 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 583, 595, 2902 |
| PctI | GAATGC | 2 cut(s) | 1504, 3568 |
| PdmI | GAANNNNTTC | 1 cut(s) | 3210 |
| PfeI | GAWTC | 8 cut(s) | 386, 614, 941, 1094, 1298, 1622, 2152, 3188 |
| PfoI | TCCNGGA | 1 cut(s) | 3667 |
| PleI | GAGTC | 8 cut(s) | 130, 504, 582, 770, 1279, 1622, 2755, 3298 |
| PpsI | GAGTC | 8 cut(s) | 130, 504, 582, 770, 1279, 1622, 2755, 3298 |
| Ppu21I | YACGTR | 1 cut(s) | 631 |
| PpuMI | RGGWCCY | 1 cut(s) | 1642 |
| PshBI | ATTAAT | 1 cut(s) | 1410 |
| Psp1406I | AACGTT | 1 cut(s) | 3593 |
| Psp5II | RGGWCCY | 1 cut(s) | 1642 |
| PspFI | CCCAGC | 1 cut(s) | 2096 |
| PspN4I | GGNNCC | 8 cut(s) | 287, 1643, 1895, 2127, 2725, 3600, 3666, 3708 |
| PspOMI | GGGCCC | 2 cut(s) | 3598, 3706 |
| PspPI | GGNCC | 8 cut(s) | 1642, 2077, 2105, 2724, 3598, 3599, 3706, 3707 |
| PspPPI | RGGWCCY | 1 cut(s) | 1642 |
| PstI | CTGCAG | 2 cut(s) | 1429, 3457 |
| PstNI | CAGNNNCTG | 3 cut(s) | 185, 1424, 1978 |
| PsuI | RGATCY | 5 cut(s) | 1797, 2683, 2968, 3498, 3765 |
| PvuII | CAGCTG | 1 cut(s) | 1421 |
| RruI | TCGCGA | 1 cut(s) | 133 |
| RsaI | GTAC | 6 cut(s) | 629, 1001, 2052, 2275, 2649, 2899 |
| RsaNI | GTAC | 6 cut(s) | 628, 1000, 2051, 2274, 2648, 2898 |
| RseI | CAYNNNNRTG | 4 cut(s) | 924, 1050, 1988, 3507 |
| SapI | GCTCTTC | 2 cut(s) | 365, 3360 |
| SaqAI | TTAA | 7 cut(s) | 1205, 1410, 2691, 2766, 3102, 3108, 3746 |
| Sau96I | GGNCC | 8 cut(s) | 1642, 2077, 2105, 2724, 3598, 3599, 3706, 3707 |
| SchI | GAGTC | 8 cut(s) | 131, 505, 583, 771, 1279, 1622, 2756, 3298 |
| SduI | GDGCHC | 3 cut(s) | 1070, 3602, 3710 |
| SfaNI | GCATC | 8 cut(s) | 24, 32, 1381, 1467, 1800, 2659, 2941, 3499 |
| SfcI | CTRYAG | 3 cut(s) | 1425, 3291, 3453 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2910 |
| SfuI | TTCGAA | 1 cut(s) | 3214 |
| SinI | GGWCC | 2 cut(s) | 1642, 2077 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 924, 1050, 1988, 3507 |
| SmlI | CTYRAG | 4 cut(s) | 1646, 1801, 2590, 2690 |
| SmoI | CTYRAG | 4 cut(s) | 1646, 1801, 2590, 2690 |
| SspI | AATATT | 1 cut(s) | 2419 |
| SspMI | CTAG | 6 cut(s) | 428, 471, 2811, 2819, 2955, 3567 |
| StyI | CCWWGG | 2 cut(s) | 2178, 3073 |
| TaaI | ACNGT | 7 cut(s) | 1084, 1507, 2159, 2440, 2888, 3338, 3720 |
| TaiI | ACGT | 5 cut(s) | 633, 832, 1344, 2899, 3596 |
| TatI | WGTACW | 4 cut(s) | 999, 2050, 2273, 2647 |
| TauI | GCSGC | 4 cut(s) | 40, 979, 3140, 3467 |
| TfiI | GAWTC | 8 cut(s) | 386, 614, 941, 1094, 1298, 1622, 2152, 3188 |
| Tru1I | TTAA | 7 cut(s) | 1205, 1410, 2691, 2766, 3102, 3108, 3746 |
| Tru9I | TTAA | 7 cut(s) | 1205, 1410, 2691, 2766, 3102, 3108, 3746 |
| TscAI | CASTG | 8 cut(s) | 28, 96, 121, 201, 1983, 2164, 2806, 3439 |
| TseFI | GTSAC | 5 cut(s) | 60, 85, 112, 578, 3577 |
| Tsp45I | GTSAC | 5 cut(s) | 60, 85, 112, 578, 3577 |
| TspGWI | ACGGA | 6 cut(s) | 278, 368, 1162, 1392, 1757, 2226 |
| TspRI | CASTG | 8 cut(s) | 28, 96, 121, 201, 1983, 2164, 2806, 3439 |
| Vha464I | CTTAAG | 1 cut(s) | 2690 |
| VpaK11BI | GGWCC | 2 cut(s) | 1642, 2077 |
| VspI | ATTAAT | 1 cut(s) | 1410 |
| XapI | RAATTY | 5 cut(s) | 321, 1773, 2634, 2848, 3104 |
| XmiI | GTMKAC | 1 cut(s) | 2208 |
| XmnI | GAANNNNTTC | 1 cut(s) | 3210 |
| XspI | CTAG | 6 cut(s) | 428, 471, 2811, 2819, 2955, 3567 |