DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1715 |
| AccB1I | GGYRCC | 2 cut(s) | 1720, 3194 |
| AccBSI | CCGCTC | 1 cut(s) | 2732 |
| AccII | CGCG | 3 cut(s) | 69, 217, 2080 |
| AccIII | TCCGGA | 1 cut(s) | 2602 |
| AciI | CCGC | 7 cut(s) | 556, 1026, 1363, 1846, 2732, 2924, 3346 |
| AclI | AACGTT | 1 cut(s) | 2528 |
| AclWI | GGATC | 8 cut(s) | 72, 549, 600, 1037, 1630, 1680, 1741, 2286 |
| AcoI | YGGCCR | 1 cut(s) | 2018 |
| AcsI | RAATTY | 4 cut(s) | 1063, 1369, 1512, 2699 |
| AcuI | CTGAAG | 2 cut(s) | 186, 1101 |
| AfaI | GTAC | 7 cut(s) | 581, 913, 1779, 1852, 2478, 2494, 2887 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 61, 697, 1233, 1805, 1878, 2062, 2251, 2936, 3008 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2097, 2129 |
| Alw21I | GWGCWC | 2 cut(s) | 133, 3397 |
| Alw26I | GTCTC | 6 cut(s) | 114, 189, 1031, 1590, 1790, 2309 |
| AlwI | GGATC | 8 cut(s) | 72, 549, 600, 1037, 1630, 1680, 1741, 2286 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3269 |
| Ama87I | CYCGRG | 2 cut(s) | 2657, 3042 |
| Aor13HI | TCCGGA | 1 cut(s) | 2602 |
| AoxI | GGCC | 5 cut(s) | 1700, 2018, 2319, 2497, 3301 |
| ApaI | GGGCCC | 1 cut(s) | 3305 |
| ApoI | RAATTY | 4 cut(s) | 1063, 1369, 1512, 2699 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1964, 1996 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 882, 1737 |
| AspLEI | GCGC | 2 cut(s) | 217, 2080 |
| AspS9I | GGNCC | 6 cut(s) | 1237, 1700, 2319, 3266, 3301, 3302 |
| AsuC2I | CCSGG | 1 cut(s) | 2276 |
| AsuHPI | GGTGA | 8 cut(s) | 44, 439, 1172, 1309, 1396, 2219, 2449, 3217 |
| AsuNHI | GCTAGC | 2 cut(s) | 1022, 3023 |
| AvaI | CYCGRG | 2 cut(s) | 2657, 3042 |
| AvaII | GGWCC | 2 cut(s) | 1237, 3266 |
| AxyI | CCTNAGG | 1 cut(s) | 892 |
| BaeGI | GKGCMC | 1 cut(s) | 3305 |
| BanI | GGYRCC | 2 cut(s) | 1720, 3194 |
| BanII | GRGCYC | 3 cut(s) | 650, 3305, 3397 |
| BbsI | GAAGAC | 1 cut(s) | 3363 |
| Bbv12I | GWGCWC | 2 cut(s) | 133, 3397 |
| BbvCI | CCTCAGC | 1 cut(s) | 1658 |
| BccI | CCATC | 5 cut(s) | 947, 1139, 1691, 3159, 3230 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 971, 1005 |
| BciVI | GTATCC | 1 cut(s) | 2816 |
| BclI | TGATCA | 3 cut(s) | 529, 1333, 2439 |
| BcnI | CCSGG | 1 cut(s) | 2276 |
| BcoDI | GTCTC | 6 cut(s) | 114, 189, 1031, 1590, 1790, 2309 |
| BfaI | CTAG | 6 cut(s) | 1023, 1998, 2550, 2883, 3024, 3162 |
| BfmI | CTRYAG | 3 cut(s) | 1104, 1578, 3060 |
| BfuI | GTATCC | 1 cut(s) | 2816 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1708 |
| BglII | AGATCT | 2 cut(s) | 1392, 2563 |
| BlpI | GCTNAGC | 1 cut(s) | 3396 |
| Bme18I | GGWCC | 2 cut(s) | 1237, 3266 |
| BmeT110I | CYCGRG | 2 cut(s) | 2657, 3042 |
| BmgT120I | GGNCC | 6 cut(s) | 1237, 1700, 2319, 3266, 3301, 3302 |
| BmiI | GGNNCC | 5 cut(s) | 1238, 1722, 2320, 3196, 3303 |
| BmrI | ACTGGG | 1 cut(s) | 1790 |
| BmtI | GCTAGC | 2 cut(s) | 1026, 3027 |
| BmuI | ACTGGG | 1 cut(s) | 1790 |
| BpiI | GAAGAC | 1 cut(s) | 3363 |
| BpmI | CTGGAG | 4 cut(s) | 1713, 2544, 3234, 3291 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1566, 1658, 2745 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3396 |
| BpuEI | CTTGAG | 2 cut(s) | 1262, 1381 |
| BpuMI | CCSGG | 1 cut(s) | 2276 |
| Bsa29I | ATCGAT | 1 cut(s) | 525 |
| BsaBI | GATNNNNATC | 2 cut(s) | 63, 465 |
| BsaI | GGTCTC | 2 cut(s) | 114, 1790 |
| BsaWI | WCCGGW | 1 cut(s) | 2602 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 595, 625 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 61, 697, 1233, 1805, 1878, 2062, 2251, 2936, 3008 |
| Bse1I | ACTGG | 9 cut(s) | 1501, 1506, 1624, 1785, 2245, 2439, 2680, 3013, 3217 |
| Bse21I | CCTNAGG | 1 cut(s) | 892 |
| Bse3DI | GCAATG | 2 cut(s) | 3034, 3114 |
| Bse8I | GATNNNNATC | 2 cut(s) | 63, 465 |
| BseAI | TCCGGA | 1 cut(s) | 2602 |
| BseCI | ATCGAT | 1 cut(s) | 525 |
| BseJI | GATNNNNATC | 2 cut(s) | 63, 465 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 61, 697, 1233, 1805, 1878, 2062, 2251, 2936, 3008 |
| BseMI | GCAATG | 2 cut(s) | 3034, 3114 |
| BseNI | ACTGG | 9 cut(s) | 1501, 1506, 1624, 1785, 2245, 2439, 2680, 3013, 3217 |
| BseRI | GAGGAG | 9 cut(s) | 61, 490, 710, 713, 766, 1049, 1186, 2723, 2740 |
| BseSI | GKGCMC | 1 cut(s) | 3305 |
| BsgI | GTGCAG | 1 cut(s) | 975 |
| Bsh1236I | CGCG | 3 cut(s) | 69, 217, 2080 |
| BshFI | GGCC | 5 cut(s) | 1702, 2020, 2321, 2499, 3303 |
| BshNI | GGYRCC | 2 cut(s) | 1720, 3194 |
| BshVI | ATCGAT | 1 cut(s) | 525 |
| BsiHKAI | GWGCWC | 2 cut(s) | 133, 3397 |
| BsiHKCI | CYCGRG | 2 cut(s) | 2657, 3042 |
| BsiSI | CCGG | 3 cut(s) | 1429, 2275, 2603 |
| BslFI | GGGAC | 8 cut(s) | 380, 910, 1250, 2233, 2438, 2667, 2686, 3019 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 61, 697, 1233, 1805, 1878, 2062, 2251, 2936, 3008 |
| BsmAI | GTCTC | 6 cut(s) | 114, 189, 1031, 1590, 1790, 2309 |
| BsmFI | GGGAC | 8 cut(s) | 380, 910, 1250, 2233, 2438, 2667, 2686, 3019 |
| BsmI | GAATGC | 2 cut(s) | 3163, 3374 |
| BsnI | GGCC | 5 cut(s) | 1702, 2020, 2321, 2499, 3303 |
| Bso31I | GGTCTC | 2 cut(s) | 114, 1790 |
| BsoBI | CYCGRG | 2 cut(s) | 2657, 3042 |
| Bsp120I | GGGCCC | 1 cut(s) | 3301 |
| Bsp1286I | GDGCHC | 4 cut(s) | 133, 650, 3305, 3397 |
| Bsp13I | TCCGGA | 1 cut(s) | 2602 |
| Bsp1407I | TGTACA | 1 cut(s) | 2476 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3396 |
| Bsp19I | CCATGG | 1 cut(s) | 2668 |
| Bsp68I | TCGCGA | 1 cut(s) | 69 |
| BspACI | CCGC | 7 cut(s) | 556, 1026, 1363, 1846, 2732, 2924, 3346 |
| BspANI | GGCC | 5 cut(s) | 1702, 2020, 2321, 2499, 3303 |
| BspDI | ATCGAT | 1 cut(s) | 525 |
| BspEI | TCCGGA | 1 cut(s) | 2602 |
| BspFNI | CGCG | 3 cut(s) | 69, 217, 2080 |
| BspLI | GGNNCC | 5 cut(s) | 1238, 1722, 2320, 3196, 3303 |
| BspMAI | CTGCAG | 2 cut(s) | 1108, 3064 |
| BspOI | GCTAGC | 2 cut(s) | 1026, 3027 |
| BspPI | GGATC | 8 cut(s) | 72, 549, 600, 1037, 1630, 1680, 1741, 2286 |
| BspT107I | GGYRCC | 2 cut(s) | 1720, 3194 |
| BspTNI | GGTCTC | 2 cut(s) | 114, 1790 |
| BsrBI | CCGCTC | 1 cut(s) | 2732 |
| BsrDI | GCAATG | 2 cut(s) | 3034, 3114 |
| BsrGI | TGTACA | 1 cut(s) | 2476 |
| BsrI | ACTGG | 9 cut(s) | 1501, 1506, 1624, 1785, 2245, 2439, 2680, 3013, 3217 |
| BssT1I | CCWWGG | 3 cut(s) | 1773, 2668, 2965 |
| Bst4CI | ACNGT | 9 cut(s) | 125, 206, 1100, 1457, 2035, 2483, 2936, 2943, 3315 |
| Bst6I | CTCTTC | 8 cut(s) | 146, 162, 251, 314, 391, 431, 1407, 2958 |
| BstAUI | TGTACA | 1 cut(s) | 2476 |
| BstC8I | GCNNGC | 6 cut(s) | 1024, 1426, 2664, 2838, 3025, 3281 |
| BstDSI | CCRYGG | 2 cut(s) | 124, 2668 |
| BstEII | GGTNACC | 1 cut(s) | 3172 |
| BstFNI | CGCG | 3 cut(s) | 69, 217, 2080 |
| BstHHI | GCGC | 2 cut(s) | 217, 2080 |
| BstMAI | GTCTC | 6 cut(s) | 114, 189, 1031, 1590, 1790, 2309 |
| BstNSI | RCATGY | 2 cut(s) | 824, 1428 |
| BstPI | GGTNACC | 1 cut(s) | 3172 |
| BstSFI | CTRYAG | 3 cut(s) | 1104, 1578, 3060 |
| BstSLI | GKGCMC | 1 cut(s) | 3305 |
| BstUI | CGCG | 3 cut(s) | 69, 217, 2080 |
| BstV2I | GAAGAC | 1 cut(s) | 3363 |
| BstX2I | RGATCY | 4 cut(s) | 1392, 1746, 2278, 2563 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1780, 2675 |
| BstYI | RGATCY | 4 cut(s) | 1392, 1746, 2278, 2563 |
| Bsu15I | ATCGAT | 1 cut(s) | 525 |
| Bsu36I | CCTNAGG | 1 cut(s) | 892 |
| BsuI | GTATCC | 1 cut(s) | 2816 |
| BsuRI | GGCC | 5 cut(s) | 1702, 2020, 2321, 2499, 3303 |
| BsuTUI | ATCGAT | 1 cut(s) | 525 |
| BtgI | CCRYGG | 2 cut(s) | 124, 2668 |
| BtsIMutI | CAGTG | 2 cut(s) | 211, 1105 |
| BtuMI | TCGCGA | 1 cut(s) | 69 |
| Cac8I | GCNNGC | 6 cut(s) | 1024, 1426, 2664, 2838, 3025, 3281 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3269 |
| CfoI | GCGC | 2 cut(s) | 217, 2080 |
| Cfr13I | GGNCC | 6 cut(s) | 1237, 1700, 2319, 3266, 3301, 3302 |
| ClaI | ATCGAT | 1 cut(s) | 525 |
| CseI | GACGC | 1 cut(s) | 1118 |
| Csp6I | GTAC | 7 cut(s) | 580, 912, 1778, 1851, 2477, 2493, 2886 |
| CviQI | GTAC | 7 cut(s) | 580, 912, 1778, 1851, 2477, 2493, 2886 |
| DraI | TTTAAA | 1 cut(s) | 2698 |
| EaeI | YGGCCR | 1 cut(s) | 2018 |
| Eam1104I | CTCTTC | 8 cut(s) | 146, 162, 251, 314, 391, 431, 1407, 2958 |
| EarI | CTCTTC | 8 cut(s) | 146, 162, 251, 314, 391, 431, 1407, 2958 |
| Ecl136II | GAGCTC | 1 cut(s) | 3395 |
| Eco130I | CCWWGG | 3 cut(s) | 1773, 2668, 2965 |
| Eco24I | GRGCYC | 3 cut(s) | 650, 3305, 3397 |
| Eco31I | GGTCTC | 2 cut(s) | 114, 1790 |
| Eco32I | GATATC | 2 cut(s) | 523, 3410 |
| Eco47I | GGWCC | 2 cut(s) | 1237, 3266 |
| Eco53kI | GAGCTC | 1 cut(s) | 3395 |
| Eco57I | CTGAAG | 2 cut(s) | 186, 1101 |
| Eco81I | CCTNAGG | 1 cut(s) | 892 |
| Eco88I | CYCGRG | 2 cut(s) | 2657, 3042 |
| Eco91I | GGTNACC | 1 cut(s) | 3172 |
| EcoICRI | GAGCTC | 1 cut(s) | 3395 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1237, 3266, 3301, 3302 |
| EcoO65I | GGTNACC | 1 cut(s) | 3172 |
| EcoRI | GAATTC | 1 cut(s) | 1063 |
| EcoRV | GATATC | 2 cut(s) | 523, 3410 |
| EcoT14I | CCWWGG | 3 cut(s) | 1773, 2668, 2965 |
| EcoT22I | ATGCAT | 1 cut(s) | 3376 |
| EcoT38I | GRGCYC | 3 cut(s) | 650, 3305, 3397 |
| ErhI | CCWWGG | 3 cut(s) | 1773, 2668, 2965 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 921, 953, 3272, 3304 |
| FaqI | GGGAC | 8 cut(s) | 380, 910, 1250, 2233, 2438, 2667, 2686, 3019 |
| FauI | CCCGC | 3 cut(s) | 1019, 1839, 3339 |
| FauNDI | CATATG | 1 cut(s) | 627 |
| FbaI | TGATCA | 3 cut(s) | 529, 1333, 2439 |
| FriOI | GRGCYC | 3 cut(s) | 650, 3305, 3397 |
| FspBI | CTAG | 6 cut(s) | 1023, 1998, 2550, 2883, 3024, 3162 |
| GlaI | GCGC | 2 cut(s) | 216, 2079 |
| GsuI | CTGGAG | 4 cut(s) | 1713, 2544, 3234, 3291 |
| HaeIII | GGCC | 5 cut(s) | 1702, 2020, 2321, 2499, 3303 |
| HapII | CCGG | 3 cut(s) | 1429, 2275, 2603 |
| HgaI | GACGC | 1 cut(s) | 1118 |
| HhaI | GCGC | 2 cut(s) | 217, 2080 |
| Hin6I | GCGC | 2 cut(s) | 215, 2078 |
| HinP1I | GCGC | 2 cut(s) | 215, 2078 |
| HpaII | CCGG | 3 cut(s) | 1429, 2275, 2603 |
| HphI | GGTGA | 8 cut(s) | 44, 439, 1172, 1309, 1396, 2219, 2449, 3217 |
| Hpy166II | GTNNAC | 4 cut(s) | 1163, 2346, 2479, 2939 |
| Hpy8I | GTNNAC | 4 cut(s) | 1163, 2346, 2479, 2939 |
| Hpy99I | CGWCG | 1 cut(s) | 138 |
| HpyCH4III | ACNGT | 9 cut(s) | 125, 206, 1100, 1457, 2035, 2483, 2936, 2943, 3315 |
| HpyCH4IV | ACGT | 3 cut(s) | 136, 2491, 2528 |
| HpySE526I | ACGT | 3 cut(s) | 136, 2491, 2528 |
| HspAI | GCGC | 2 cut(s) | 215, 2078 |
| Kpn2I | TCCGGA | 1 cut(s) | 2602 |
| Ksp22I | TGATCA | 3 cut(s) | 529, 1333, 2439 |
| LmnI | GCTCC | 6 cut(s) | 442, 1010, 2425, 2563, 3215, 3392 |
| MaeI | CTAG | 6 cut(s) | 1023, 1998, 2550, 2883, 3024, 3162 |
| MaeII | ACGT | 3 cut(s) | 136, 2491, 2528 |
| MaeIII | GTNAC | 5 cut(s) | 50, 1572, 1933, 2644, 3172 |
| MbiI | CCGCTC | 1 cut(s) | 2732 |
| MfeI | CAATTG | 1 cut(s) | 1436 |
| MflI | RGATCY | 4 cut(s) | 1392, 1746, 2278, 2563 |
| MhlI | GDGCHC | 4 cut(s) | 133, 650, 3305, 3397 |
| MlyI | GAGTC | 5 cut(s) | 58, 847, 1414, 2351, 2649 |
| MmeI | TCCRAC | 3 cut(s) | 2070, 2467, 2489 |
| Mph1103I | ATGCAT | 1 cut(s) | 3376 |
| MroI | TCCGGA | 1 cut(s) | 2602 |
| MroXI | GAANNNNTTC | 2 cut(s) | 882, 1737 |
| MslI | CAYNNNNRTG | 4 cut(s) | 504, 564, 825, 2295 |
| MspA1I | CMGCKG | 1 cut(s) | 989 |
| MspI | CCGG | 3 cut(s) | 1429, 2275, 2603 |
| MunI | CAATTG | 1 cut(s) | 1436 |
| Mva1269I | GAATGC | 2 cut(s) | 3163, 3374 |
| MvnI | CGCG | 3 cut(s) | 69, 217, 2080 |
| NciI | CCSGG | 1 cut(s) | 2276 |
| NcoI | CCATGG | 1 cut(s) | 2668 |
| NdeI | CATATG | 1 cut(s) | 627 |
| NheI | GCTAGC | 2 cut(s) | 1022, 3023 |
| NlaIV | GGNNCC | 5 cut(s) | 1238, 1722, 2320, 3196, 3303 |
| NmeAIII | GCCGAG | 2 cut(s) | 248, 2525 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| NruI | TCGCGA | 1 cut(s) | 69 |
| NsiI | ATGCAT | 1 cut(s) | 3376 |
| NspI | RCATGY | 2 cut(s) | 824, 1428 |
| PaeI | GCATGC | 1 cut(s) | 1428 |
| PaeR7I | CTCGAG | 1 cut(s) | 2657 |
| PasI | CCCWGGG | 1 cut(s) | 3002 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2023, 2497 |
| PctI | GAATGC | 2 cut(s) | 3163, 3374 |
| PdmI | GAANNNNTTC | 2 cut(s) | 882, 1737 |
| PfoI | TCCNGGA | 1 cut(s) | 2274 |
| PleI | GAGTC | 5 cut(s) | 57, 847, 1413, 2350, 2649 |
| PpsI | GAGTC | 5 cut(s) | 57, 847, 1413, 2350, 2649 |
| PpuMI | RGGWCCY | 2 cut(s) | 1237, 3266 |
| PsiI | TTATAA | 1 cut(s) | 1715 |
| Psp124BI | GAGCTC | 1 cut(s) | 3397 |
| Psp1406I | AACGTT | 1 cut(s) | 2528 |
| Psp5II | RGGWCCY | 2 cut(s) | 1237, 3266 |
| PspEI | GGTNACC | 1 cut(s) | 3172 |
| PspN4I | GGNNCC | 5 cut(s) | 1238, 1722, 2320, 3196, 3303 |
| PspOMI | GGGCCC | 1 cut(s) | 3301 |
| PspPI | GGNCC | 6 cut(s) | 1237, 1700, 2319, 3266, 3301, 3302 |
| PspPPI | RGGWCCY | 2 cut(s) | 1237, 3266 |
| PspXI | VCTCGAGB | 1 cut(s) | 2657 |
| PstI | CTGCAG | 2 cut(s) | 1108, 3064 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3269 |
| PsuI | RGATCY | 4 cut(s) | 1392, 1746, 2278, 2563 |
| PvuII | CAGCTG | 1 cut(s) | 989 |
| RruI | TCGCGA | 1 cut(s) | 69 |
| RsaI | GTAC | 7 cut(s) | 581, 913, 1779, 1852, 2478, 2494, 2887 |
| RsaNI | GTAC | 7 cut(s) | 580, 912, 1778, 1851, 2477, 2493, 2886 |
| RseI | CAYNNNNRTG | 4 cut(s) | 504, 564, 825, 2295 |
| SacI | GAGCTC | 1 cut(s) | 3397 |
| Sau96I | GGNCC | 6 cut(s) | 1237, 1700, 2319, 3266, 3301, 3302 |
| SchI | GAGTC | 5 cut(s) | 58, 847, 1414, 2351, 2649 |
| SduI | GDGCHC | 4 cut(s) | 133, 650, 3305, 3397 |
| SfcI | CTRYAG | 3 cut(s) | 1104, 1578, 3060 |
| Sfr274I | CTCGAG | 1 cut(s) | 2657 |
| SinI | GGWCC | 2 cut(s) | 1237, 3266 |
| SlaI | CTCGAG | 1 cut(s) | 2657 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 504, 564, 825, 2295 |
| SmlI | CTYRAG | 3 cut(s) | 1241, 1396, 2657 |
| SmoI | CTYRAG | 3 cut(s) | 1241, 1396, 2657 |
| SphI | GCATGC | 1 cut(s) | 1428 |
| SsiI | CCGC | 7 cut(s) | 556, 1026, 1363, 1846, 2732, 2924, 3346 |
| SspI | AATATT | 1 cut(s) | 2014 |
| SspMI | CTAG | 6 cut(s) | 1023, 1998, 2550, 2883, 3024, 3162 |
| SstI | GAGCTC | 1 cut(s) | 3397 |
| StyI | CCWWGG | 3 cut(s) | 1773, 2668, 2965 |
| TaaI | ACNGT | 9 cut(s) | 125, 206, 1100, 1457, 2035, 2483, 2936, 2943, 3315 |
| TaiI | ACGT | 3 cut(s) | 139, 2494, 2531 |
| TatI | WGTACW | 3 cut(s) | 579, 2476, 2885 |
| TauI | GCSGC | 2 cut(s) | 559, 2735 |
| TscAI | CASTG | 2 cut(s) | 211, 1105 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspRI | CASTG | 2 cut(s) | 211, 1105 |
| VpaK11BI | GGWCC | 2 cut(s) | 1237, 3266 |
| XapI | RAATTY | 4 cut(s) | 1063, 1369, 1512, 2699 |
| XceI | RCATGY | 2 cut(s) | 824, 1428 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1513 |
| XhoI | CTCGAG | 1 cut(s) | 2657 |
| XmnI | GAANNNNTTC | 2 cut(s) | 882, 1737 |
| XspI | CTAG | 6 cut(s) | 1023, 1998, 2550, 2883, 3024, 3162 |
| Zsp2I | ATGCAT | 1 cut(s) | 3376 |