DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1634 |
| AccB1I | GGYRCC | 2 cut(s) | 1639, 3113 |
| AccBSI | CCGCTC | 3 cut(s) | 511, 2651, 3137 |
| AccII | CGCG | 2 cut(s) | 205, 568 |
| AciI | CCGC | 9 cut(s) | 472, 511, 566, 945, 1765, 2041, 2651, 3137, 3265 |
| AclI | AACGTT | 1 cut(s) | 1856 |
| AclWI | GGATC | 6 cut(s) | 66, 956, 1549, 1599, 2205, 2352 |
| AcoI | YGGCCR | 3 cut(s) | 1937, 2425, 3138 |
| AcsI | RAATTY | 4 cut(s) | 982, 1431, 2025, 2618 |
| AcuI | CTGAAG | 1 cut(s) | 1020 |
| AfaI | GTAC | 6 cut(s) | 497, 829, 1789, 1812, 2397, 2413 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 240, 613, 755, 1152, 1724, 1797, 1981, 2170, 2855 |
| AgsI | TTSAA | 9 cut(s) | 776, 1007, 1366, 1904, 1928, 1988, 2078, 2351, 3017 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3153 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2016, 2048 |
| Alw21I | GWGCWC | 1 cut(s) | 3316 |
| Alw26I | GTCTC | 8 cut(s) | 177, 406, 815, 950, 1103, 1509, 2228, 2736 |
| AlwI | GGATC | 6 cut(s) | 66, 956, 1549, 1599, 2205, 2352 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3188 |
| Ama87I | CYCGRG | 1 cut(s) | 2961 |
| AoxI | GGCC | 7 cut(s) | 1619, 1937, 2238, 2416, 2425, 3138, 3220 |
| ApaI | GGGCCC | 1 cut(s) | 3224 |
| ApoI | RAATTY | 4 cut(s) | 982, 1431, 2025, 2618 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1883, 1915 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 798, 2724 |
| AspLEI | GCGC | 3 cut(s) | 207, 2587, 2665 |
| AspS9I | GGNCC | 6 cut(s) | 109, 1619, 2238, 3185, 3220, 3221 |
| AsuC2I | CCSGG | 1 cut(s) | 56 |
| AsuHPI | GGTGA | 8 cut(s) | 44, 343, 355, 455, 1091, 1315, 2138, 2368 |
| AsuII | TTCGAA | 2 cut(s) | 1461, 2728 |
| AsuNHI | GCTAGC | 1 cut(s) | 941 |
| AvaI | CYCGRG | 1 cut(s) | 2961 |
| AvaII | GGWCC | 2 cut(s) | 109, 3185 |
| AxyI | CCTNAGG | 1 cut(s) | 808 |
| BaeGI | GKGCMC | 1 cut(s) | 3224 |
| BanI | GGYRCC | 2 cut(s) | 1639, 3113 |
| BanII | GRGCYC | 3 cut(s) | 566, 3224, 3316 |
| BbsI | GAAGAC | 2 cut(s) | 1229, 3282 |
| Bbv12I | GWGCWC | 1 cut(s) | 3316 |
| BccI | CCATC | 9 cut(s) | 86, 538, 646, 863, 1039, 1610, 2945, 3078, 3149 |
| BclI | TGATCA | 2 cut(s) | 445, 2358 |
| BcnI | CCSGG | 1 cut(s) | 56 |
| BcoDI | GTCTC | 8 cut(s) | 177, 406, 815, 950, 1103, 1509, 2228, 2736 |
| BfaI | CTAG | 4 cut(s) | 942, 1181, 2469, 3081 |
| BfmI | CTRYAG | 3 cut(s) | 1023, 1245, 2979 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1627, 2424 |
| BglII | AGATCT | 2 cut(s) | 1311, 2482 |
| BlpI | GCTNAGC | 1 cut(s) | 3315 |
| Bme18I | GGWCC | 2 cut(s) | 109, 3185 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3153 |
| BmeT110I | CYCGRG | 1 cut(s) | 2961 |
| BmgT120I | GGNCC | 6 cut(s) | 109, 1619, 2238, 3185, 3220, 3221 |
| BmiI | GGNNCC | 5 cut(s) | 110, 1641, 2239, 3115, 3222 |
| BmrI | ACTGGG | 1 cut(s) | 2931 |
| BmsI | GCATC | 8 cut(s) | 412, 961, 1033, 1417, 1530, 2455, 2918, 3013 |
| BmtI | GCTAGC | 1 cut(s) | 945 |
| BmuI | ACTGGG | 1 cut(s) | 2931 |
| BpiI | GAAGAC | 2 cut(s) | 1229, 3282 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1226, 1258 |
| BpmI | CTGGAG | 4 cut(s) | 1632, 2463, 3153, 3210 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1485, 1577, 2204 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3315 |
| Bpu14I | TTCGAA | 2 cut(s) | 1461, 2728 |
| BpuEI | CTTGAG | 2 cut(s) | 383, 2125 |
| BpuMI | CCSGG | 1 cut(s) | 56 |
| BsaBI | GATNNNNATC | 1 cut(s) | 381 |
| BsaI | GGTCTC | 1 cut(s) | 1103 |
| BsaJI | CCNNGG | 6 cut(s) | 1127, 1692, 1792, 2169, 2884, 3150 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2850, 2880 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 240, 613, 755, 1152, 1724, 1797, 1981, 2170, 2855 |
| Bse21I | CCTNAGG | 1 cut(s) | 808 |
| Bse3DI | GCAATG | 2 cut(s) | 2953, 3033 |
| Bse8I | GATNNNNATC | 1 cut(s) | 381 |
| BseDI | CCNNGG | 6 cut(s) | 1127, 1692, 1792, 2169, 2884, 3150 |
| BseJI | GATNNNNATC | 1 cut(s) | 381 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 240, 613, 755, 1152, 1724, 1797, 1981, 2170, 2855 |
| BseMI | GCAATG | 2 cut(s) | 2953, 3033 |
| BseRI | GAGGAG | 9 cut(s) | 61, 111, 327, 406, 626, 629, 662, 679, 1105 |
| BseSI | GKGCMC | 1 cut(s) | 3224 |
| Bsh1236I | CGCG | 2 cut(s) | 205, 568 |
| BshFI | GGCC | 7 cut(s) | 1621, 1939, 2240, 2418, 2427, 3140, 3222 |
| BshNI | GGYRCC | 2 cut(s) | 1639, 3113 |
| BsiHKAI | GWGCWC | 1 cut(s) | 3316 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2961 |
| BsiSI | CCGG | 3 cut(s) | 55, 1348, 1936 |
| BslFI | GGGAC | 8 cut(s) | 122, 269, 1169, 1700, 2152, 2357, 2504, 2938 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 240, 613, 755, 1152, 1724, 1797, 1981, 2170, 2855 |
| BsmAI | GTCTC | 8 cut(s) | 177, 406, 815, 950, 1103, 1509, 2228, 2736 |
| BsmFI | GGGAC | 8 cut(s) | 122, 269, 1169, 1700, 2152, 2357, 2504, 2938 |
| BsmI | GAATGC | 3 cut(s) | 742, 3082, 3293 |
| BsnI | GGCC | 7 cut(s) | 1621, 1939, 2240, 2418, 2427, 3140, 3222 |
| Bso31I | GGTCTC | 1 cut(s) | 1103 |
| BsoBI | CYCGRG | 1 cut(s) | 2961 |
| Bsp119I | TTCGAA | 2 cut(s) | 1461, 2728 |
| Bsp120I | GGGCCC | 1 cut(s) | 3220 |
| Bsp1286I | GDGCHC | 3 cut(s) | 566, 3224, 3316 |
| Bsp1407I | TGTACA | 1 cut(s) | 2395 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3315 |
| BspACI | CCGC | 9 cut(s) | 472, 511, 566, 945, 1765, 2041, 2651, 3137, 3265 |
| BspANI | GGCC | 7 cut(s) | 1621, 1939, 2240, 2418, 2427, 3140, 3222 |
| BspFNI | CGCG | 2 cut(s) | 205, 568 |
| BspLI | GGNNCC | 5 cut(s) | 110, 1641, 2239, 3115, 3222 |
| BspMAI | CTGCAG | 2 cut(s) | 1027, 2983 |
| BspOI | GCTAGC | 1 cut(s) | 945 |
| BspPI | GGATC | 6 cut(s) | 66, 956, 1549, 1599, 2205, 2352 |
| BspQI | GCTCTTC | 1 cut(s) | 2641 |
| BspT104I | TTCGAA | 2 cut(s) | 1461, 2728 |
| BspT107I | GGYRCC | 2 cut(s) | 1639, 3113 |
| BspTNI | GGTCTC | 1 cut(s) | 1103 |
| BsrBI | CCGCTC | 3 cut(s) | 511, 2651, 3137 |
| BsrDI | GCAATG | 2 cut(s) | 2953, 3033 |
| BsrGI | TGTACA | 1 cut(s) | 2395 |
| BssECI | CCNNGG | 6 cut(s) | 1127, 1692, 1792, 2169, 2884, 3150 |
| BssT1I | CCWWGG | 2 cut(s) | 1692, 2884 |
| Bst4CI | ACNGT | 6 cut(s) | 113, 194, 1954, 2402, 2855, 3234 |
| Bst6I | CTCTTC | 7 cut(s) | 239, 260, 347, 664, 1326, 2622, 2641 |
| BstAUI | TGTACA | 1 cut(s) | 2395 |
| BstBI | TTCGAA | 2 cut(s) | 1461, 2728 |
| BstC8I | GCNNGC | 7 cut(s) | 566, 943, 1345, 2425, 2583, 2667, 2757 |
| BstEII | GGTNACC | 1 cut(s) | 3091 |
| BstFNI | CGCG | 2 cut(s) | 205, 568 |
| BstHHI | GCGC | 3 cut(s) | 207, 2587, 2665 |
| BstMAI | GTCTC | 8 cut(s) | 177, 406, 815, 950, 1103, 1509, 2228, 2736 |
| BstNSI | RCATGY | 1 cut(s) | 1347 |
| BstPI | GGTNACC | 1 cut(s) | 3091 |
| BstSFI | CTRYAG | 3 cut(s) | 1023, 1245, 2979 |
| BstSLI | GKGCMC | 1 cut(s) | 3224 |
| BstUI | CGCG | 2 cut(s) | 205, 568 |
| BstV2I | GAAGAC | 2 cut(s) | 1229, 3282 |
| BstX2I | RGATCY | 4 cut(s) | 1311, 2197, 2344, 2482 |
| BstYI | RGATCY | 4 cut(s) | 1311, 2197, 2344, 2482 |
| Bsu36I | CCTNAGG | 1 cut(s) | 808 |
| BsuRI | GGCC | 7 cut(s) | 1621, 1939, 2240, 2418, 2427, 3140, 3222 |
| BtsI | GCAGTG | 1 cut(s) | 70 |
| BtsIMutI | CAGTG | 4 cut(s) | 70, 199, 920, 2919 |
| Cac8I | GCNNGC | 7 cut(s) | 566, 943, 1345, 2425, 2583, 2667, 2757 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3188 |
| CfoI | GCGC | 3 cut(s) | 207, 2587, 2665 |
| Cfr13I | GGNCC | 6 cut(s) | 109, 1619, 2238, 3185, 3220, 3221 |
| CseI | GACGC | 1 cut(s) | 211 |
| Csp6I | GTAC | 6 cut(s) | 496, 828, 1788, 1811, 2396, 2412 |
| CviQI | GTAC | 6 cut(s) | 496, 828, 1788, 1811, 2396, 2412 |
| DraI | TTTAAA | 1 cut(s) | 2617 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3153 |
| EaeI | YGGCCR | 3 cut(s) | 1937, 2425, 3138 |
| Eam1104I | CTCTTC | 7 cut(s) | 239, 260, 347, 664, 1326, 2622, 2641 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3153 |
| EarI | CTCTTC | 7 cut(s) | 239, 260, 347, 664, 1326, 2622, 2641 |
| EciI | GGCGGA | 1 cut(s) | 2056 |
| Ecl136II | GAGCTC | 1 cut(s) | 3314 |
| Eco130I | CCWWGG | 2 cut(s) | 1692, 2884 |
| Eco24I | GRGCYC | 3 cut(s) | 566, 3224, 3316 |
| Eco31I | GGTCTC | 1 cut(s) | 1103 |
| Eco32I | GATATC | 4 cut(s) | 1678, 2216, 2271, 3329 |
| Eco47I | GGWCC | 2 cut(s) | 109, 3185 |
| Eco53kI | GAGCTC | 1 cut(s) | 3314 |
| Eco57I | CTGAAG | 1 cut(s) | 1020 |
| Eco81I | CCTNAGG | 1 cut(s) | 808 |
| Eco88I | CYCGRG | 1 cut(s) | 2961 |
| Eco91I | GGTNACC | 1 cut(s) | 3091 |
| EcoICRI | GAGCTC | 1 cut(s) | 3314 |
| EcoO109I | RGGNCCY | 3 cut(s) | 3185, 3220, 3221 |
| EcoO65I | GGTNACC | 1 cut(s) | 3091 |
| EcoRI | GAATTC | 1 cut(s) | 982 |
| EcoRV | GATATC | 4 cut(s) | 1678, 2216, 2271, 3329 |
| EcoT14I | CCWWGG | 2 cut(s) | 1692, 2884 |
| EcoT22I | ATGCAT | 1 cut(s) | 3295 |
| EcoT38I | GRGCYC | 3 cut(s) | 566, 3224, 3316 |
| ErhI | CCWWGG | 2 cut(s) | 1692, 2884 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 162, 194, 837, 869, 3191, 3223 |
| FaqI | GGGAC | 8 cut(s) | 122, 269, 1169, 1700, 2152, 2357, 2504, 2938 |
| FauI | CCCGC | 5 cut(s) | 504, 573, 938, 1758, 3258 |
| FbaI | TGATCA | 2 cut(s) | 445, 2358 |
| FriOI | GRGCYC | 3 cut(s) | 566, 3224, 3316 |
| FspBI | CTAG | 4 cut(s) | 942, 1181, 2469, 3081 |
| GlaI | GCGC | 3 cut(s) | 206, 2586, 2664 |
| GsuI | CTGGAG | 4 cut(s) | 1632, 2463, 3153, 3210 |
| HaeIII | GGCC | 7 cut(s) | 1621, 1939, 2240, 2418, 2427, 3140, 3222 |
| HapII | CCGG | 3 cut(s) | 55, 1348, 1936 |
| HgaI | GACGC | 1 cut(s) | 211 |
| HhaI | GCGC | 3 cut(s) | 207, 2587, 2665 |
| Hin6I | GCGC | 3 cut(s) | 205, 2585, 2663 |
| HinP1I | GCGC | 3 cut(s) | 205, 2585, 2663 |
| HincII | GTYRAC | 1 cut(s) | 1252 |
| HindII | GTYRAC | 1 cut(s) | 1252 |
| HindIII | AAGCTT | 1 cut(s) | 176 |
| HpaII | CCGG | 3 cut(s) | 55, 1348, 1936 |
| HphI | GGTGA | 8 cut(s) | 44, 343, 355, 455, 1091, 1315, 2138, 2368 |
| Hpy166II | GTNNAC | 5 cut(s) | 535, 1082, 1252, 2265, 2398 |
| Hpy8I | GTNNAC | 5 cut(s) | 535, 1082, 1252, 2265, 2398 |
| Hpy99I | CGWCG | 1 cut(s) | 126 |
| HpyAV | CCTTC | 7 cut(s) | 22, 280, 697, 770, 1300, 2714, 3082 |
| HpyCH4III | ACNGT | 6 cut(s) | 113, 194, 1954, 2402, 2855, 3234 |
| HpyCH4IV | ACGT | 4 cut(s) | 124, 1809, 1856, 2410 |
| HpySE526I | ACGT | 4 cut(s) | 124, 1809, 1856, 2410 |
| HspAI | GCGC | 3 cut(s) | 205, 2585, 2663 |
| Ksp22I | TGATCA | 2 cut(s) | 445, 2358 |
| LguI | GCTCTTC | 1 cut(s) | 2641 |
| LmnI | GCTCC | 8 cut(s) | 358, 457, 913, 929, 1651, 2482, 3134, 3311 |
| LweI | GCATC | 8 cut(s) | 412, 961, 1033, 1417, 1530, 2455, 2918, 3013 |
| MaeI | CTAG | 4 cut(s) | 942, 1181, 2469, 3081 |
| MaeII | ACGT | 4 cut(s) | 124, 1809, 1856, 2410 |
| MaeIII | GTNAC | 4 cut(s) | 50, 1852, 2563, 3091 |
| MbiI | CCGCTC | 3 cut(s) | 511, 2651, 3137 |
| MfeI | CAATTG | 3 cut(s) | 912, 1035, 1355 |
| MflI | RGATCY | 4 cut(s) | 1311, 2197, 2344, 2482 |
| MhlI | GDGCHC | 3 cut(s) | 566, 3224, 3316 |
| MlyI | GAGTC | 5 cut(s) | 58, 763, 1333, 2270, 2560 |
| MmeI | TCCRAC | 2 cut(s) | 1989, 2386 |
| Mph1103I | ATGCAT | 1 cut(s) | 3295 |
| MroXI | GAANNNNTTC | 2 cut(s) | 798, 2724 |
| MseI | TTAA | 9 cut(s) | 594, 692, 1398, 1760, 2280, 2490, 2616, 2622, 3260 |
| MslI | CAYNNNNRTG | 3 cut(s) | 420, 542, 2877 |
| MspI | CCGG | 3 cut(s) | 55, 1348, 1936 |
| MunI | CAATTG | 3 cut(s) | 912, 1035, 1355 |
| Mva1269I | GAATGC | 3 cut(s) | 742, 3082, 3293 |
| MvnI | CGCG | 2 cut(s) | 205, 568 |
| NciI | CCSGG | 1 cut(s) | 56 |
| NheI | GCTAGC | 1 cut(s) | 941 |
| NlaIV | GGNNCC | 5 cut(s) | 110, 1641, 2239, 3115, 3222 |
| NmeAIII | GCCGAG | 2 cut(s) | 236, 2444 |
| NmuCI | GTSAC | 2 cut(s) | 50, 2563 |
| NsiI | ATGCAT | 1 cut(s) | 3295 |
| NspI | RCATGY | 1 cut(s) | 1347 |
| NspV | TTCGAA | 2 cut(s) | 1461, 2728 |
| PaeI | GCATGC | 1 cut(s) | 1347 |
| PciSI | GCTCTTC | 1 cut(s) | 2641 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2416 |
| PctI | GAATGC | 3 cut(s) | 742, 3082, 3293 |
| PdmI | GAANNNNTTC | 2 cut(s) | 798, 2724 |
| PleI | GAGTC | 5 cut(s) | 57, 763, 1332, 2269, 2560 |
| PpsI | GAGTC | 5 cut(s) | 57, 763, 1332, 2269, 2560 |
| PpuMI | RGGWCCY | 1 cut(s) | 3185 |
| PsiI | TTATAA | 1 cut(s) | 1634 |
| Psp124BI | GAGCTC | 1 cut(s) | 3316 |
| Psp1406I | AACGTT | 1 cut(s) | 1856 |
| Psp5II | RGGWCCY | 1 cut(s) | 3185 |
| PspEI | GGTNACC | 1 cut(s) | 3091 |
| PspN4I | GGNNCC | 5 cut(s) | 110, 1641, 2239, 3115, 3222 |
| PspOMI | GGGCCC | 1 cut(s) | 3220 |
| PspPI | GGNCC | 6 cut(s) | 109, 1619, 2238, 3185, 3220, 3221 |
| PspPPI | RGGWCCY | 1 cut(s) | 3185 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 109, 141 |
| PstI | CTGCAG | 2 cut(s) | 1027, 2983 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3188 |
| PsuI | RGATCY | 4 cut(s) | 1311, 2197, 2344, 2482 |
| RsaI | GTAC | 6 cut(s) | 497, 829, 1789, 1812, 2397, 2413 |
| RsaNI | GTAC | 6 cut(s) | 496, 828, 1788, 1811, 2396, 2412 |
| RseI | CAYNNNNRTG | 3 cut(s) | 420, 542, 2877 |
| SacI | GAGCTC | 1 cut(s) | 3316 |
| SapI | GCTCTTC | 1 cut(s) | 2641 |
| SaqAI | TTAA | 9 cut(s) | 594, 692, 1398, 1760, 2280, 2490, 2616, 2622, 3260 |
| Sau96I | GGNCC | 6 cut(s) | 109, 1619, 2238, 3185, 3220, 3221 |
| SchI | GAGTC | 5 cut(s) | 58, 763, 1333, 2270, 2560 |
| SduI | GDGCHC | 3 cut(s) | 566, 3224, 3316 |
| SfaNI | GCATC | 8 cut(s) | 412, 961, 1033, 1417, 1530, 2455, 2918, 3013 |
| SfcI | CTRYAG | 3 cut(s) | 1023, 1245, 2979 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2424 |
| SfuI | TTCGAA | 2 cut(s) | 1461, 2728 |
| SinI | GGWCC | 2 cut(s) | 109, 3185 |
| SmiI | ATTTAAAT | 1 cut(s) | 2617 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 420, 542, 2877 |
| SmlI | CTYRAG | 2 cut(s) | 362, 2104 |
| SmoI | CTYRAG | 2 cut(s) | 362, 2104 |
| SphI | GCATGC | 1 cut(s) | 1347 |
| SsiI | CCGC | 9 cut(s) | 472, 511, 566, 945, 1765, 2041, 2651, 3137, 3265 |
| SspMI | CTAG | 4 cut(s) | 942, 1181, 2469, 3081 |
| SstI | GAGCTC | 1 cut(s) | 3316 |
| StyI | CCWWGG | 2 cut(s) | 1692, 2884 |
| SwaI | ATTTAAAT | 1 cut(s) | 2617 |
| TaaI | ACNGT | 6 cut(s) | 113, 194, 1954, 2402, 2855, 3234 |
| TaiI | ACGT | 4 cut(s) | 127, 1812, 1859, 2413 |
| TatI | WGTACW | 2 cut(s) | 495, 2395 |
| TauI | GCSGC | 3 cut(s) | 475, 2654, 3140 |
| Tru1I | TTAA | 9 cut(s) | 594, 692, 1398, 1760, 2280, 2490, 2616, 2622, 3260 |
| Tru9I | TTAA | 9 cut(s) | 594, 692, 1398, 1760, 2280, 2490, 2616, 2622, 3260 |
| TscAI | CASTG | 4 cut(s) | 70, 199, 927, 2926 |
| TseFI | GTSAC | 2 cut(s) | 50, 2563 |
| Tsp45I | GTSAC | 2 cut(s) | 50, 2563 |
| TspGWI | ACGGA | 2 cut(s) | 676, 1271 |
| TspRI | CASTG | 4 cut(s) | 70, 199, 927, 2926 |
| VpaK11BI | GGWCC | 2 cut(s) | 109, 3185 |
| XapI | RAATTY | 4 cut(s) | 982, 1431, 2025, 2618 |
| XbaI | TCTAGA | 1 cut(s) | 1180 |
| XceI | RCATGY | 1 cut(s) | 1347 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1432 |
| XmnI | GAANNNNTTC | 2 cut(s) | 798, 2724 |
| XspI | CTAG | 4 cut(s) | 942, 1181, 2469, 3081 |
| Zsp2I | ATGCAT | 1 cut(s) | 3295 |