DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1838, 3544 |
| Acc36I | ACCTGC | 1 cut(s) | 711 |
| AccB1I | GGYRCC | 2 cut(s) | 1611, 1843 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 3059 |
| AccBSI | CCGCTC | 1 cut(s) | 583 |
| AccI | GTMKAC | 1 cut(s) | 1926 |
| AccIII | TCCGGA | 1 cut(s) | 2683 |
| AciI | CCGC | 7 cut(s) | 583, 592, 862, 1085, 1116, 1969, 3122 |
| AclWI | GGATC | 7 cut(s) | 90, 1157, 1373, 1472, 1567, 2409, 2694 |
| AcoI | YGGCCR | 2 cut(s) | 2611, 2629 |
| AcsI | RAATTY | 5 cut(s) | 1183, 1492, 1869, 2352, 2822 |
| AcuI | CTGAAG | 2 cut(s) | 1116, 1221 |
| AfaI | GTAC | 5 cut(s) | 1005, 1902, 1993, 2130, 3010 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 814, 959, 1298, 1537, 1928, 2001, 2185, 3059, 3146 |
| AflIII | ACRYGT | 1 cut(s) | 3469 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2737 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1285, 1317, 2220, 2252 |
| Alw21I | GWGCWC | 2 cut(s) | 2024, 3520 |
| Alw26I | GTCTC | 8 cut(s) | 132, 207, 261, 1019, 1713, 1913, 2432, 2745 |
| Alw44I | GTGCAC | 1 cut(s) | 2020 |
| AlwI | GGATC | 7 cut(s) | 90, 1157, 1373, 1472, 1567, 2409, 2694 |
| Ama87I | CYCGRG | 1 cut(s) | 3165 |
| Aor13HI | TCCGGA | 1 cut(s) | 2683 |
| AoxI | GGCC | 9 cut(s) | 966, 1823, 1974, 2141, 2611, 2629, 2871, 3316, 3424 |
| ApaI | GGGCCC | 2 cut(s) | 3320, 3428 |
| ApaLI | GTGCAC | 1 cut(s) | 2020 |
| ApoI | RAATTY | 5 cut(s) | 1183, 1492, 1869, 2352, 2822 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1730, 1762, 1971, 2003, 2087, 2119 |
| AspA2I | CCTAGG | 2 cut(s) | 41, 668 |
| AspLEI | GCGC | 3 cut(s) | 156, 237, 609 |
| AspS9I | GGNCC | 8 cut(s) | 1795, 1823, 1974, 3316, 3317, 3389, 3424, 3425 |
| AsuHPI | GGTGA | 6 cut(s) | 791, 1295, 1409, 1432, 2110, 2594 |
| AsuII | TTCGAA | 1 cut(s) | 2932 |
| AvaI | CYCGRG | 1 cut(s) | 3165 |
| AvaII | GGWCC | 2 cut(s) | 1795, 3389 |
| AvrII | CCTAGG | 2 cut(s) | 41, 668 |
| BaeGI | GKGCMC | 4 cut(s) | 1593, 2024, 3320, 3428 |
| BanI | GGYRCC | 2 cut(s) | 1611, 1843 |
| BanII | GRGCYC | 4 cut(s) | 758, 3320, 3428, 3520 |
| BbsI | GAAGAC | 2 cut(s) | 1056, 2598 |
| Bbv12I | GWGCWC | 2 cut(s) | 2024, 3520 |
| BbvCI | CCTCAGC | 1 cut(s) | 1781 |
| BceAI | ACGGC | 1 cut(s) | 2775 |
| BciVI | GTATCC | 1 cut(s) | 2430 |
| BclI | TGATCA | 1 cut(s) | 1456 |
| BcoDI | GTCTC | 8 cut(s) | 132, 207, 261, 1019, 1713, 1913, 2432, 2745 |
| BfaI | CTAG | 6 cut(s) | 42, 333, 669, 2673, 3006, 3095 |
| BfmI | CTRYAG | 1 cut(s) | 1449 |
| BfoI | RGCGCY | 1 cut(s) | 157 |
| BfuAI | ACCTGC | 1 cut(s) | 711 |
| BfuI | GTATCC | 1 cut(s) | 2430 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1831 |
| BglII | AGATCT | 1 cut(s) | 1515 |
| BlnI | CCTAGG | 2 cut(s) | 41, 668 |
| BlpI | GCTNAGC | 1 cut(s) | 3519 |
| Bme18I | GGWCC | 2 cut(s) | 1795, 3389 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2737 |
| BmeT110I | CYCGRG | 1 cut(s) | 3165 |
| BmgT120I | GGNCC | 8 cut(s) | 1795, 1823, 1974, 3316, 3317, 3389, 3424, 3425 |
| BmiI | GGNNCC | 6 cut(s) | 755, 1613, 1845, 1975, 3318, 3426 |
| BmrI | ACTGGG | 2 cut(s) | 1752, 3345 |
| BmsI | GCATC | 8 cut(s) | 610, 931, 1066, 1129, 1162, 2377, 2659, 3217 |
| BmuI | ACTGGG | 2 cut(s) | 1752, 3345 |
| BpiI | GAAGAC | 2 cut(s) | 1056, 2598 |
| BpmI | CTGGAG | 2 cut(s) | 842, 1836 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1689, 1781, 2408 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3519 |
| Bpu14I | TTCGAA | 1 cut(s) | 2932 |
| BpuEI | CTTGAG | 3 cut(s) | 1504, 2329, 2801 |
| BsaBI | GATNNNNATC | 4 cut(s) | 81, 573, 1377, 1476 |
| BsaI | GGTCTC | 2 cut(s) | 132, 1913 |
| BsaJI | CCNNGG | 9 cut(s) | 41, 668, 1292, 1331, 1576, 1996, 2874, 3088, 3354 |
| BsaWI | WCCGGW | 1 cut(s) | 2683 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1065, 1095, 2885, 2915 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 814, 959, 1298, 1537, 1928, 2001, 2185, 3059, 3146 |
| Bse1I | ACTGG | 7 cut(s) | 1382, 1624, 1747, 2562, 2803, 3136, 3340 |
| Bse3DI | GCAATG | 7 cut(s) | 24, 1731, 2497, 2985, 3157, 3195, 3237 |
| Bse8I | GATNNNNATC | 4 cut(s) | 81, 573, 1377, 1476 |
| BseAI | TCCGGA | 1 cut(s) | 2683 |
| BseDI | CCNNGG | 9 cut(s) | 41, 668, 1292, 1331, 1576, 1996, 2874, 3088, 3354 |
| BseGI | GGATG | 8 cut(s) | 745, 832, 850, 946, 1273, 2010, 2080, 2295 |
| BseJI | GATNNNNATC | 4 cut(s) | 81, 573, 1377, 1476 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 814, 959, 1298, 1537, 1928, 2001, 2185, 3059, 3146 |
| BseMI | GCAATG | 7 cut(s) | 24, 1731, 2497, 2985, 3157, 3195, 3237 |
| BseNI | ACTGG | 7 cut(s) | 1382, 1624, 1747, 2562, 2803, 3136, 3340 |
| BseRI | GAGGAG | 6 cut(s) | 79, 200, 827, 830, 2863, 2906 |
| BseSI | GKGCMC | 4 cut(s) | 1593, 2024, 3320, 3428 |
| BseYI | CCCAGC | 1 cut(s) | 3125 |
| BsgI | GTGCAG | 1 cut(s) | 1095 |
| BshFI | GGCC | 9 cut(s) | 968, 1825, 1976, 2143, 2613, 2631, 2873, 3318, 3426 |
| BshNI | GGYRCC | 2 cut(s) | 1611, 1843 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2024, 3520 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3165 |
| BsiSI | CCGG | 3 cut(s) | 2140, 2632, 2684 |
| BslFI | GGGAC | 6 cut(s) | 311, 626, 1373, 2790, 2809, 3142 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 814, 959, 1298, 1537, 1928, 2001, 2185, 3059, 3146 |
| BsmAI | GTCTC | 8 cut(s) | 132, 207, 261, 1019, 1713, 1913, 2432, 2745 |
| BsmFI | GGGAC | 6 cut(s) | 311, 626, 1373, 2790, 2809, 3142 |
| BsmI | GAATGC | 1 cut(s) | 3286 |
| BsnI | GGCC | 9 cut(s) | 968, 1825, 1976, 2143, 2613, 2631, 2873, 3318, 3426 |
| Bso31I | GGTCTC | 2 cut(s) | 132, 1913 |
| BsoBI | CYCGRG | 1 cut(s) | 3165 |
| Bsp119I | TTCGAA | 1 cut(s) | 2932 |
| Bsp120I | GGGCCC | 2 cut(s) | 3316, 3424 |
| Bsp1286I | GDGCHC | 6 cut(s) | 758, 1593, 2024, 3320, 3428, 3520 |
| Bsp13I | TCCGGA | 1 cut(s) | 2683 |
| Bsp1407I | TGTACA | 1 cut(s) | 2128 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3519 |
| Bsp19I | CCATGG | 1 cut(s) | 1576 |
| BspACI | CCGC | 7 cut(s) | 583, 592, 862, 1085, 1116, 1969, 3122 |
| BspANI | GGCC | 9 cut(s) | 968, 1825, 1976, 2143, 2613, 2631, 2873, 3318, 3426 |
| BspEI | TCCGGA | 1 cut(s) | 2683 |
| BspLI | GGNNCC | 6 cut(s) | 755, 1613, 1845, 1975, 3318, 3426 |
| BspMI | ACCTGC | 1 cut(s) | 711 |
| BspPI | GGATC | 7 cut(s) | 90, 1157, 1373, 1472, 1567, 2409, 2694 |
| BspT104I | TTCGAA | 1 cut(s) | 2932 |
| BspT107I | GGYRCC | 2 cut(s) | 1611, 1843 |
| BspTNI | GGTCTC | 2 cut(s) | 132, 1913 |
| BsrBI | CCGCTC | 1 cut(s) | 583 |
| BsrDI | GCAATG | 7 cut(s) | 24, 1731, 2497, 2985, 3157, 3195, 3237 |
| BsrGI | TGTACA | 1 cut(s) | 2128 |
| BsrI | ACTGG | 7 cut(s) | 1382, 1624, 1747, 2562, 2803, 3136, 3340 |
| BssECI | CCNNGG | 9 cut(s) | 41, 668, 1292, 1331, 1576, 1996, 2874, 3088, 3354 |
| BssNAI | GTATAC | 1 cut(s) | 1927 |
| BssT1I | CCWWGG | 6 cut(s) | 41, 668, 1292, 1576, 2874, 3088 |
| Bst1107I | GTATAC | 1 cut(s) | 1927 |
| Bst4CI | ACNGT | 6 cut(s) | 143, 1233, 1877, 2019, 2158, 2606 |
| Bst6I | CTCTTC | 5 cut(s) | 18, 314, 1530, 2826, 3081 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 3119, 3179 |
| BstAUI | TGTACA | 1 cut(s) | 2128 |
| BstBI | TTCGAA | 1 cut(s) | 2932 |
| BstC8I | GCNNGC | 7 cut(s) | 1446, 2203, 2625, 2629, 2787, 2961, 3404 |
| BstDSI | CCRYGG | 1 cut(s) | 1576 |
| BstEII | GGTNACC | 1 cut(s) | 3295 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 957 |
| BstF5I | GGATG | 8 cut(s) | 745, 832, 850, 946, 1273, 2010, 2080, 2295 |
| BstH2I | RGCGCY | 1 cut(s) | 157 |
| BstHHI | GCGC | 3 cut(s) | 156, 237, 609 |
| BstMAI | GTCTC | 8 cut(s) | 132, 207, 261, 1019, 1713, 1913, 2432, 2745 |
| BstPI | GGTNACC | 1 cut(s) | 3295 |
| BstSFI | CTRYAG | 1 cut(s) | 1449 |
| BstSLI | GKGCMC | 4 cut(s) | 1593, 2024, 3320, 3428 |
| BstV2I | GAAGAC | 2 cut(s) | 1056, 2598 |
| BstX2I | RGATCY | 4 cut(s) | 1515, 1572, 2401, 2686 |
| BstYI | RGATCY | 4 cut(s) | 1515, 1572, 2401, 2686 |
| BstZ17I | GTATAC | 1 cut(s) | 1927 |
| BsuI | GTATCC | 1 cut(s) | 2430 |
| BsuRI | GGCC | 9 cut(s) | 968, 1825, 1976, 2143, 2613, 2631, 2873, 3318, 3426 |
| BtgI | CCRYGG | 1 cut(s) | 1576 |
| BtgZI | GCGATG | 1 cut(s) | 2400 |
| BtsCI | GGATG | 8 cut(s) | 745, 832, 850, 946, 1273, 2010, 2080, 2295 |
| BtsI | GCAGTG | 2 cut(s) | 1701, 3064 |
| BtsIMutI | CAGTG | 3 cut(s) | 1701, 1882, 3064 |
| BveI | ACCTGC | 1 cut(s) | 711 |
| Cac8I | GCNNGC | 7 cut(s) | 1446, 2203, 2625, 2629, 2787, 2961, 3404 |
| CfoI | GCGC | 3 cut(s) | 156, 237, 609 |
| Cfr13I | GGNCC | 8 cut(s) | 1795, 1823, 1974, 3316, 3317, 3389, 3424, 3425 |
| Csp6I | GTAC | 5 cut(s) | 1004, 1901, 1992, 2129, 3009 |
| CviAII | CATG | 7 cut(s) | 209, 437, 1577, 2098, 2414, 2438, 2792 |
| CviQI | GTAC | 5 cut(s) | 1004, 1901, 1992, 2129, 3009 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2737 |
| EaeI | YGGCCR | 2 cut(s) | 2611, 2629 |
| Eam1104I | CTCTTC | 5 cut(s) | 18, 314, 1530, 2826, 3081 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2737 |
| EarI | CTCTTC | 5 cut(s) | 18, 314, 1530, 2826, 3081 |
| Ecl136II | GAGCTC | 1 cut(s) | 3518 |
| Eco130I | CCWWGG | 6 cut(s) | 41, 668, 1292, 1576, 2874, 3088 |
| Eco147I | AGGCCT | 1 cut(s) | 968 |
| Eco24I | GRGCYC | 4 cut(s) | 758, 3320, 3428, 3520 |
| Eco31I | GGTCTC | 2 cut(s) | 132, 1913 |
| Eco32I | GATATC | 2 cut(s) | 3484, 3533 |
| Eco47I | GGWCC | 2 cut(s) | 1795, 3389 |
| Eco53kI | GAGCTC | 1 cut(s) | 3518 |
| Eco57I | CTGAAG | 2 cut(s) | 1116, 1221 |
| Eco88I | CYCGRG | 1 cut(s) | 3165 |
| Eco91I | GGTNACC | 1 cut(s) | 3295 |
| EcoICRI | GAGCTC | 1 cut(s) | 3518 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 957 |
| EcoO109I | RGGNCCY | 3 cut(s) | 3317, 3424, 3425 |
| EcoO65I | GGTNACC | 1 cut(s) | 3295 |
| EcoRI | GAATTC | 1 cut(s) | 1183 |
| EcoRV | GATATC | 2 cut(s) | 3484, 3533 |
| EcoT14I | CCWWGG | 6 cut(s) | 41, 668, 1292, 1576, 2874, 3088 |
| EcoT38I | GRGCYC | 4 cut(s) | 758, 3320, 3428, 3520 |
| ErhI | CCWWGG | 6 cut(s) | 41, 668, 1292, 1576, 2874, 3088 |
| FaeI | CATG | 7 cut(s) | 212, 440, 1580, 2101, 2417, 2441, 2795 |
| FaqI | GGGAC | 6 cut(s) | 311, 626, 1373, 2790, 2809, 3142 |
| FatI | CATG | 7 cut(s) | 208, 436, 1576, 2097, 2413, 2437, 2791 |
| FauI | CCCGC | 3 cut(s) | 585, 1123, 1962 |
| FbaI | TGATCA | 1 cut(s) | 1456 |
| FblI | GTMKAC | 1 cut(s) | 1926 |
| FokI | GGATG | 8 cut(s) | 752, 839, 857, 953, 1280, 2017, 2087, 2302 |
| FriOI | GRGCYC | 4 cut(s) | 758, 3320, 3428, 3520 |
| FspBI | CTAG | 6 cut(s) | 42, 333, 669, 2673, 3006, 3095 |
| GlaI | GCGC | 3 cut(s) | 155, 236, 608 |
| GsaI | CCCAGC | 1 cut(s) | 3129 |
| GsuI | CTGGAG | 2 cut(s) | 842, 1836 |
| HaeII | RGCGCY | 1 cut(s) | 157 |
| HaeIII | GGCC | 9 cut(s) | 968, 1825, 1976, 2143, 2613, 2631, 2873, 3318, 3426 |
| HapII | CCGG | 3 cut(s) | 2140, 2632, 2684 |
| HhaI | GCGC | 3 cut(s) | 156, 237, 609 |
| Hin1II | CATG | 7 cut(s) | 212, 440, 1580, 2101, 2417, 2441, 2795 |
| Hin6I | GCGC | 3 cut(s) | 154, 235, 607 |
| HinP1I | GCGC | 3 cut(s) | 154, 235, 607 |
| HindIII | AAGCTT | 2 cut(s) | 1442, 1655 |
| HpaII | CCGG | 3 cut(s) | 2140, 2632, 2684 |
| HphI | GGTGA | 6 cut(s) | 791, 1295, 1409, 1432, 2110, 2594 |
| Hpy166II | GTNNAC | 8 cut(s) | 1286, 1893, 1927, 2022, 2469, 2602, 3062, 3333 |
| Hpy8I | GTNNAC | 8 cut(s) | 1286, 1893, 1927, 2022, 2469, 2602, 3062, 3333 |
| HpyCH4III | ACNGT | 6 cut(s) | 143, 1233, 1877, 2019, 2158, 2606 |
| HpyCH4IV | ACGT | 1 cut(s) | 3469 |
| HpySE526I | ACGT | 1 cut(s) | 3469 |
| Hsp92II | CATG | 7 cut(s) | 212, 440, 1580, 2101, 2417, 2441, 2795 |
| HspAI | GCGC | 3 cut(s) | 154, 235, 607 |
| Kpn2I | TCCGGA | 1 cut(s) | 2683 |
| Ksp22I | TGATCA | 1 cut(s) | 1456 |
| LweI | GCATC | 8 cut(s) | 610, 931, 1066, 1129, 1162, 2377, 2659, 3217 |
| MaeI | CTAG | 6 cut(s) | 42, 333, 669, 2673, 3006, 3095 |
| MaeII | ACGT | 1 cut(s) | 3469 |
| MaeIII | GTNAC | 3 cut(s) | 2056, 3295, 3382 |
| MbiI | CCGCTC | 1 cut(s) | 583 |
| MfeI | CAATTG | 3 cut(s) | 1236, 1559, 3177 |
| MflI | RGATCY | 4 cut(s) | 1515, 1572, 2401, 2686 |
| MhlI | GDGCHC | 6 cut(s) | 758, 1593, 2024, 3320, 3428, 3520 |
| MlyI | GAGTC | 5 cut(s) | 76, 967, 1340, 2378, 2474 |
| MmeI | TCCRAC | 2 cut(s) | 1051, 2682 |
| MroI | TCCGGA | 1 cut(s) | 2683 |
| MseI | TTAA | 7 cut(s) | 893, 1659, 1884, 2213, 2484, 2826, 3464 |
| MslI | CAYNNNNRTG | 1 cut(s) | 738 |
| MspA1I | CMGCKG | 2 cut(s) | 1109, 3185 |
| MspI | CCGG | 3 cut(s) | 2140, 2632, 2684 |
| MunI | CAATTG | 3 cut(s) | 1236, 1559, 3177 |
| Mva1269I | GAATGC | 1 cut(s) | 3286 |
| NcoI | CCATGG | 1 cut(s) | 1576 |
| NlaIII | CATG | 7 cut(s) | 212, 440, 1580, 2101, 2417, 2441, 2795 |
| NlaIV | GGNNCC | 6 cut(s) | 755, 1613, 1845, 1975, 3318, 3426 |
| NspV | TTCGAA | 1 cut(s) | 2932 |
| PceI | AGGCCT | 1 cut(s) | 968 |
| PctI | GAATGC | 1 cut(s) | 3286 |
| PfeI | GAWTC | 8 cut(s) | 56, 179, 782, 986, 1340, 2581, 2906, 3217 |
| PflFI | GACNNNGTC | 1 cut(s) | 1744 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 3059 |
| PleI | GAGTC | 5 cut(s) | 75, 967, 1340, 2377, 2473 |
| PpsI | GAGTC | 5 cut(s) | 75, 967, 1340, 2377, 2473 |
| PsiI | TTATAA | 2 cut(s) | 1838, 3544 |
| Psp124BI | GAGCTC | 1 cut(s) | 3520 |
| PspEI | GGTNACC | 1 cut(s) | 3295 |
| PspFI | CCCAGC | 1 cut(s) | 3125 |
| PspN4I | GGNNCC | 6 cut(s) | 755, 1613, 1845, 1975, 3318, 3426 |
| PspOMI | GGGCCC | 2 cut(s) | 3316, 3424 |
| PspPI | GGNCC | 8 cut(s) | 1795, 1823, 1974, 3316, 3317, 3389, 3424, 3425 |
| PsuI | RGATCY | 4 cut(s) | 1515, 1572, 2401, 2686 |
| PsyI | GACNNNGTC | 1 cut(s) | 1744 |
| PvuII | CAGCTG | 2 cut(s) | 1109, 3185 |
| RsaI | GTAC | 5 cut(s) | 1005, 1902, 1993, 2130, 3010 |
| RsaNI | GTAC | 5 cut(s) | 1004, 1901, 1992, 2129, 3009 |
| RseI | CAYNNNNRTG | 1 cut(s) | 738 |
| SacI | GAGCTC | 1 cut(s) | 3520 |
| SaqAI | TTAA | 7 cut(s) | 893, 1659, 1884, 2213, 2484, 2826, 3464 |
| Sau96I | GGNCC | 8 cut(s) | 1795, 1823, 1974, 3316, 3317, 3389, 3424, 3425 |
| SchI | GAGTC | 5 cut(s) | 76, 967, 1340, 2378, 2474 |
| SduI | GDGCHC | 6 cut(s) | 758, 1593, 2024, 3320, 3428, 3520 |
| SfaNI | GCATC | 8 cut(s) | 610, 931, 1066, 1129, 1162, 2377, 2659, 3217 |
| SfcI | CTRYAG | 1 cut(s) | 1449 |
| SfuI | TTCGAA | 1 cut(s) | 2932 |
| SinI | GGWCC | 2 cut(s) | 1795, 3389 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 738 |
| SmlI | CTYRAG | 3 cut(s) | 1519, 2308, 2780 |
| SmoI | CTYRAG | 3 cut(s) | 1519, 2308, 2780 |
| SseBI | AGGCCT | 1 cut(s) | 968 |
| SsiI | CCGC | 7 cut(s) | 583, 592, 862, 1085, 1116, 1969, 3122 |
| SspMI | CTAG | 6 cut(s) | 42, 333, 669, 2673, 3006, 3095 |
| SstI | GAGCTC | 1 cut(s) | 3520 |
| StuI | AGGCCT | 1 cut(s) | 968 |
| StyI | CCWWGG | 6 cut(s) | 41, 668, 1292, 1576, 2874, 3088 |
| TaaI | ACNGT | 6 cut(s) | 143, 1233, 1877, 2019, 2158, 2606 |
| TaiI | ACGT | 1 cut(s) | 3472 |
| TaqI | TCGA | 7 cut(s) | 73, 1273, 1717, 2086, 2932, 3286, 3420 |
| TatI | WGTACW | 2 cut(s) | 2128, 3008 |
| TauI | GCSGC | 1 cut(s) | 3125 |
| TfiI | GAWTC | 8 cut(s) | 56, 179, 782, 986, 1340, 2581, 2906, 3217 |
| Tru1I | TTAA | 7 cut(s) | 893, 1659, 1884, 2213, 2484, 2826, 3464 |
| Tru9I | TTAA | 7 cut(s) | 893, 1659, 1884, 2213, 2484, 2826, 3464 |
| TscAI | CASTG | 3 cut(s) | 1701, 1882, 3064 |
| TspGWI | ACGGA | 2 cut(s) | 850, 1944 |
| TspRI | CASTG | 3 cut(s) | 1701, 1882, 3064 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1744 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 3059 |
| VneI | GTGCAC | 1 cut(s) | 2020 |
| VpaK11BI | GGWCC | 2 cut(s) | 1795, 3389 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 957 |
| XapI | RAATTY | 5 cut(s) | 1183, 1492, 1869, 2352, 2822 |
| XmaJI | CCTAGG | 2 cut(s) | 41, 668 |
| XmiI | GTMKAC | 1 cut(s) | 1926 |
| XspI | CTAG | 6 cut(s) | 42, 333, 669, 2673, 3006, 3095 |