DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 116, 177 |
| AfaI | GTAC | 1 cut(s) | 202 |
| AflIII | ACRYGT | 1 cut(s) | 139 |
| AgsI | TTSAA | 3 cut(s) | 176, 262, 327 |
| ApoI | RAATTY | 2 cut(s) | 116, 177 |
| AspLEI | GCGC | 1 cut(s) | 230 |
| AsuHPI | GGTGA | 1 cut(s) | 69 |
| BauI | CACGAG | 1 cut(s) | 189 |
| BciVI | GTATCC | 2 cut(s) | 239, 306 |
| BfuI | GTATCC | 2 cut(s) | 239, 306 |
| BsaJI | CCNNGG | 1 cut(s) | 121 |
| Bse1I | ACTGG | 1 cut(s) | 74 |
| BseDI | CCNNGG | 1 cut(s) | 121 |
| BseNI | ACTGG | 1 cut(s) | 74 |
| BsiWI | CGTACG | 1 cut(s) | 200 |
| BsmI | GAATGC | 1 cut(s) | 103 |
| BsrI | ACTGG | 1 cut(s) | 74 |
| BssECI | CCNNGG | 1 cut(s) | 121 |
| BssSI | CACGAG | 1 cut(s) | 189 |
| BssT1I | CCWWGG | 1 cut(s) | 121 |
| Bst2BI | CACGAG | 1 cut(s) | 189 |
| Bst4CI | ACNGT | 1 cut(s) | 299 |
| BstC8I | GCNNGC | 1 cut(s) | 207 |
| BstHHI | GCGC | 1 cut(s) | 230 |
| BstNSI | RCATGY | 3 cut(s) | 143, 209, 253 |
| BsuI | GTATCC | 2 cut(s) | 239, 306 |
| Cac8I | GCNNGC | 1 cut(s) | 207 |
| CfoI | GCGC | 1 cut(s) | 230 |
| Csp6I | GTAC | 1 cut(s) | 201 |
| CviAII | CATG | 3 cut(s) | 140, 206, 250 |
| CviJI | RGCY | 4 cut(s) | 21, 66, 242, 281 |
| CviKI_1 | RGCY | 4 cut(s) | 21, 66, 242, 281 |
| CviQI | GTAC | 1 cut(s) | 201 |
| Eco130I | CCWWGG | 1 cut(s) | 121 |
| Eco32I | GATATC | 1 cut(s) | 75 |
| EcoRI | GAATTC | 1 cut(s) | 116 |
| EcoRV | GATATC | 1 cut(s) | 75 |
| EcoT14I | CCWWGG | 1 cut(s) | 121 |
| EcoT22I | ATGCAT | 1 cut(s) | 211 |
| ErhI | CCWWGG | 1 cut(s) | 121 |
| FaeI | CATG | 3 cut(s) | 143, 209, 253 |
| FaiI | YATR | 8 cut(s) | 44, 61, 90, 141, 207, 211, 251, 355 |
| FatI | CATG | 3 cut(s) | 139, 205, 249 |
| GlaI | GCGC | 1 cut(s) | 229 |
| HhaI | GCGC | 1 cut(s) | 230 |
| Hin1II | CATG | 3 cut(s) | 143, 209, 253 |
| Hin6I | GCGC | 1 cut(s) | 228 |
| HinP1I | GCGC | 1 cut(s) | 228 |
| HinfI | GANTC | 1 cut(s) | 161 |
| HphI | GGTGA | 1 cut(s) | 69 |
| Hpy188I | TCNGA | 1 cut(s) | 183 |
| Hpy188III | TCNNGA | 3 cut(s) | 33, 310, 327 |
| HpyCH4III | ACNGT | 1 cut(s) | 299 |
| HpyCH4V | TGCA | 2 cut(s) | 101, 209 |
| Hsp92II | CATG | 3 cut(s) | 143, 209, 253 |
| HspAI | GCGC | 1 cut(s) | 228 |
| LmnI | GCTCC | 1 cut(s) | 233 |
| LpnPI | CCDG | 5 cut(s) | 41, 55, 216, 228, 295 |
| MaeIII | GTNAC | 2 cut(s) | 135, 269 |
| MboII | GAAGA | 1 cut(s) | 137 |
| MfeI | CAATTG | 1 cut(s) | 102 |
| MluCI | AATT | 4 cut(s) | 102, 116, 177, 349 |
| Mph1103I | ATGCAT | 1 cut(s) | 211 |
| MseI | TTAA | 1 cut(s) | 111 |
| MunI | CAATTG | 1 cut(s) | 102 |
| Mva1269I | GAATGC | 1 cut(s) | 103 |
| NlaIII | CATG | 3 cut(s) | 143, 209, 253 |
| NmuCI | GTSAC | 1 cut(s) | 135 |
| NsiI | ATGCAT | 1 cut(s) | 211 |
| NspI | RCATGY | 3 cut(s) | 143, 209, 253 |
| PaeI | GCATGC | 1 cut(s) | 209 |
| PciI | ACATGT | 1 cut(s) | 139 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 196 |
| PctI | GAATGC | 1 cut(s) | 103 |
| PfeI | GAWTC | 1 cut(s) | 161 |
| Pfl23II | CGTACG | 1 cut(s) | 200 |
| PscI | ACATGT | 1 cut(s) | 139 |
| PspLI | CGTACG | 1 cut(s) | 200 |
| RsaI | GTAC | 1 cut(s) | 202 |
| RsaNI | GTAC | 1 cut(s) | 201 |
| SaqAI | TTAA | 1 cut(s) | 111 |
| SetI | ASST | 2 cut(s) | 82, 336 |
| SphI | GCATGC | 1 cut(s) | 209 |
| Sse9I | AATT | 4 cut(s) | 102, 116, 177, 349 |
| StyI | CCWWGG | 1 cut(s) | 121 |
| TaaI | ACNGT | 1 cut(s) | 299 |
| TasI | AATT | 4 cut(s) | 102, 116, 177, 349 |
| TfiI | GAWTC | 1 cut(s) | 161 |
| Tru1I | TTAA | 1 cut(s) | 111 |
| Tru9I | TTAA | 1 cut(s) | 111 |
| TseFI | GTSAC | 1 cut(s) | 135 |
| Tsp45I | GTSAC | 1 cut(s) | 135 |
| TspDTI | ATGAA | 1 cut(s) | 129 |
| XapI | RAATTY | 2 cut(s) | 116, 177 |
| XceI | RCATGY | 3 cut(s) | 143, 209, 253 |
| Zsp2I | ATGCAT | 1 cut(s) | 211 |