AT3G12750

zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002306
Physical Location & Seq
Reverse (-)
4051651 .. 4053201
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12750.1

Sequence Viewer

Length: 1068 bp
ATGTCTGAATGTGGATGTTTTTCGGCAACAACTATGTTGAGAATTTGTGTAGTATTGATAATATGTTTGCATATGTGTTGTGCCTCGAGTGATTGTACAAGTCACGATGATCCTGTGTCTCAAGACGAAGCAGAGAAAGCGACGAAGCTAAAGCTTGGTTCGATAGCTTTACTTCTTGTAGCCGGAGGAGTCGGCGTGAGTCTACCGTTGATCGGGAAAAGGATTCCGGCGTTACAACCGGAAAATGATATCTTCTTCATGGTGAAAGCTTTTGCTGCAGGAGTGATCCTCTGCACAGGTTTCGTTCATATCTTACCAGACGCGTTCGAGAGATTGAGCTCTCCATGTCTTGAGGACACTACAGCTGGGAAGTTCCCGTTTGCTGGTTTTGTAGCGATGCTGTCGGCGATGGGGACTCTTATGATCGACACATTCGCGACAGGGTATTACAAGAGGCAACATTTTAGCAATAACCATGGGAGCAAGCAAGTGAACGTAGTAGTAGATGAAGAAGAGCATGCGGGTCATGTTCACATTCACACGCACGCTAGTCACGGACACACACATGGTTCGACCGAGTTGATCAGAAGACGTATAGTGTCGCAGGTGCTTGAGATTGGGATAGTTGTGCATTCGGTTATTATAGGGATATCACTTGGAGCTTCACAGAGCATAGACACCATAAAGCCACTCATGGCTGCACTATCTTTCCATCAGTTCTTTGAAGGTCTTGGCCTCGGTGGATGCATCTCCCTGGCGGATATGAAGTCGAAATCGACAGTGCTAATGGCGACATTTTTCTCGGTGACGGCGCCACTTGGGATAGGAATAGGGTTGGGGATGTCAAGTGGTTTAGGCTACAGGAAAGAGAGCAAAGAGGCAATAATGGTGGAAGGAATGTTGAATGCTGCATCTGCTGGGATACTCATATACATGTCACTTGTTGATCTTCTTGCTACTGATTTTATGAATCCAAGATTGCAATCCAATCTCTGGCTTCACTTGGCTGCTTATCTCTCTCTCGTCCTAGGCGCAGGTTCCATGTCTCTCCTCGCCATCTGGGCCTGA

Protein Analysis

355

Amino Acids

37.81

Weight (kDa)

6.22

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 49 - 352 2.5e-81 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 595
Acc36I ACCTGC 2 cut(s) 595, 1025
AccB1I GGYRCC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 993
AccI GTMKAC 1 cut(s) 202
AccII CGCG 2 cut(s) 323, 437
AciI CCGC 2 cut(s) 521, 758
AclWI GGATC 2 cut(s) 104, 280
AcsI RAATTY 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 812
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 3 cut(s) 212, 383, 993
AflIII ACRYGT 2 cut(s) 321, 933
AgsI TTSAA 2 cut(s) 725, 904
AjnI CCWGG 1 cut(s) 753
AluBI AGCT 7 cut(s) 148, 154, 167, 269, 339, 365, 662
AluI AGCT 7 cut(s) 148, 154, 167, 269, 339, 365, 662
Alw21I GWGCWC 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 123, 1050
AlwI GGATC 2 cut(s) 104, 280
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 2 cut(s) 733, 1062
ApeKI GCWGC 4 cut(s) 275, 698, 908, 1007
ApoI RAATTY 1 cut(s) 42
AspA2I CCTAGG 1 cut(s) 1027
AspLEI GCGC 2 cut(s) 814, 1034
AspS9I GGNCC 1 cut(s) 1062
AsuHPI GGTGA 2 cut(s) 274, 817
AvaI CYCGRG 1 cut(s) 85
AvrII CCTAGG 1 cut(s) 1027
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 595
Bbv12I GWGCWC 1 cut(s) 341
BbvI GCAGC 4 cut(s) 262, 685, 895, 994
BccI CCATC 3 cut(s) 403, 720, 1064
BceAI ACGGC 1 cut(s) 825
BcgI CGANNNNNNTGC 2 cut(s) 283, 317
BciT130I CCWGG 1 cut(s) 755
BciVI GTATCC 1 cut(s) 915
BclI TGATCA 1 cut(s) 582
BcoDI GTCTC 2 cut(s) 123, 1050
BfaI CTAG 2 cut(s) 549, 1028
BfmI CTRYAG 3 cut(s) 276, 360, 859
BfoI RGCGCY 1 cut(s) 815
BfuAI ACCTGC 2 cut(s) 595, 1025
BfuI GTATCC 1 cut(s) 915
BglI GCCNNNNNGGC 1 cut(s) 1061
BisI GCNGC 4 cut(s) 276, 699, 909, 1008
BlnI CCTAGG 1 cut(s) 1027
BlsI GCNGC 4 cut(s) 277, 700, 910, 1009
Bme1390I CCNGG 1 cut(s) 755
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 1062
BmiI GGNNCC 2 cut(s) 813, 1039
BmrFI CCNGG 1 cut(s) 755
BmsI GCATC 4 cut(s) 387, 734, 756, 920
BpiI GAAGAC 1 cut(s) 595
BplI GAGNNNNNCTC 2 cut(s) 273, 305
BpuEI CTTGAG 3 cut(s) 105, 371, 632
BsaBI GATNNNNATC 1 cut(s) 982
BsaHI GRCGYC 1 cut(s) 812
BsaJI CCNNGG 4 cut(s) 475, 736, 753, 1027
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 383, 993
Bse8I GATNNNNATC 1 cut(s) 982
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 4 cut(s) 475, 736, 753, 1027
BseGI GGATG 3 cut(s) 20, 749, 846
BseJI GATNNNNATC 1 cut(s) 982
BseLI CCNNNNNNNGG 3 cut(s) 212, 383, 993
BseRI GAGGAG 2 cut(s) 201, 1040
BseXI GCAGC 4 cut(s) 262, 685, 895, 994
BseYI CCCAGC 2 cut(s) 365, 917
BsgI GTGCAG 2 cut(s) 277, 684
Bsh1236I CGCG 2 cut(s) 323, 437
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 735, 1064
BshNI GGYRCC 1 cut(s) 811
BsiEI CGRYCG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 341
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 3 cut(s) 183, 227, 239
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 3 cut(s) 212, 383, 993
BsmAI GTCTC 2 cut(s) 123, 1050
BsmFI GGGAC 1 cut(s) 427
BsmI GAATGC 2 cut(s) 631, 910
BsnI GGCC 2 cut(s) 735, 1064
BsoBI CYCGRG 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 341
Bsp1407I TGTACA 1 cut(s) 95
Bsp143I GATC 6 cut(s) 109, 210, 285, 423, 582, 946
Bsp19I CCATGG 1 cut(s) 475
Bsp68I TCGCGA 1 cut(s) 437
BspACI CCGC 2 cut(s) 521, 758
BspANI GGCC 2 cut(s) 735, 1064
BspFNI CGCG 2 cut(s) 323, 437
BspLI GGNNCC 2 cut(s) 813, 1039
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 2 cut(s) 595, 1025
BspPI GGATC 2 cut(s) 104, 280
BspQI GCTCTTC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 811
BsrGI TGTACA 1 cut(s) 95
BssECI CCNNGG 4 cut(s) 475, 736, 753, 1027
BssMI GATC 6 cut(s) 109, 210, 285, 423, 582, 946
BssNI GRCGYC 1 cut(s) 812
BssT1I CCWWGG 2 cut(s) 475, 1027
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 2 cut(s) 207, 781
Bst6I CTCTTC 1 cut(s) 507
BstACI GRCGYC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 95
BstC8I GCNNGC 3 cut(s) 485, 519, 546
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 3 cut(s) 20, 749, 846
BstFNI CGCG 2 cut(s) 323, 437
BstH2I RGCGCY 1 cut(s) 815
BstHHI GCGC 2 cut(s) 814, 1034
BstKTI GATC 6 cut(s) 112, 213, 288, 426, 585, 949
BstMAI GTCTC 2 cut(s) 123, 1050
BstMBI GATC 6 cut(s) 109, 210, 285, 423, 582, 946
BstMCI CGRYCG 1 cut(s) 576
BstMWI GCNNNNNNNGC 4 cut(s) 137, 275, 914, 1061
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 2 cut(s) 521, 937
BstSCI CCNGG 1 cut(s) 753
BstSFI CTRYAG 3 cut(s) 276, 360, 859
BstUI CGCG 2 cut(s) 323, 437
BstV1I GCAGC 4 cut(s) 262, 685, 895, 994
BstV2I GAAGAC 1 cut(s) 595
BsuI GTATCC 1 cut(s) 915
BsuRI GGCC 2 cut(s) 735, 1064
BtgI CCRYGG 1 cut(s) 475
BtgZI GCGATG 2 cut(s) 410, 422
BtsCI GGATG 3 cut(s) 20, 749, 846
BtsIMutI CAGTG 1 cut(s) 786
BtuMI TCGCGA 1 cut(s) 437
BveI ACCTGC 2 cut(s) 595, 1025
Cac8I GCNNGC 3 cut(s) 485, 519, 546
CfoI GCGC 2 cut(s) 814, 1034
Cfr13I GGNCC 1 cut(s) 1062
CseI GACGC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 96
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 9 cut(s) 259, 345, 476, 518, 527, 566, 694, 934, 1042
CviQI GTAC 1 cut(s) 96
DinI GGCGCC 1 cut(s) 813
DpnI GATC 6 cut(s) 111, 212, 287, 425, 584, 948
DpnII GATC 6 cut(s) 109, 210, 285, 423, 582, 946
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 1 cut(s) 773
Ecl136II GAGCTC 1 cut(s) 339
Eco130I CCWWGG 2 cut(s) 475, 1027
Eco24I GRGCYC 1 cut(s) 341
Eco32I GATATC 2 cut(s) 250, 651
Eco53kI GAGCTC 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 753
EcoRV GATATC 2 cut(s) 250, 651
EcoT14I CCWWGG 2 cut(s) 475, 1027
EcoT22I ATGCAT 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 341
EgeI GGCGCC 1 cut(s) 813
EheI GGCGCC 1 cut(s) 813
ErhI CCWWGG 2 cut(s) 475, 1027
FaeI CATG 9 cut(s) 262, 348, 479, 521, 530, 569, 697, 937, 1045
FaqI GGGAC 1 cut(s) 427
FatI CATG 9 cut(s) 258, 344, 475, 517, 526, 565, 693, 933, 1041
FauI CCCGC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 72
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 1 cut(s) 202
Fnu4HI GCNGC 4 cut(s) 276, 699, 909, 1008
FokI GGATG 3 cut(s) 27, 756, 853
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 276, 699, 909, 1008
FspBI CTAG 2 cut(s) 549, 1028
GlaI GCGC 2 cut(s) 813, 1033
GluI GCNGC 4 cut(s) 276, 699, 909, 1008
GsaI CCCAGC 2 cut(s) 369, 921
HaeII RGCGCY 1 cut(s) 815
HaeIII GGCC 2 cut(s) 735, 1064
HapII CCGG 3 cut(s) 183, 227, 239
HgaI GACGC 1 cut(s) 329
HhaI GCGC 2 cut(s) 814, 1034
Hin1I GRCGYC 1 cut(s) 812
Hin1II CATG 9 cut(s) 262, 348, 479, 521, 530, 569, 697, 937, 1045
Hin6I GCGC 2 cut(s) 812, 1032
HinP1I GCGC 2 cut(s) 812, 1032
HindIII AAGCTT 2 cut(s) 152, 267
HinfI GANTC 5 cut(s) 189, 199, 223, 415, 970
HpaII CCGG 3 cut(s) 183, 227, 239
HphI GGTGA 2 cut(s) 274, 817
Hpy166II GTNNAC 3 cut(s) 203, 493, 532
Hpy188I TCNGA 2 cut(s) 7, 587
Hpy188III TCNNGA 6 cut(s) 104, 122, 214, 328, 350, 436
Hpy8I GTNNAC 3 cut(s) 203, 493, 532
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 2 cut(s) 719, 887
HpyCH4III ACNGT 2 cut(s) 207, 781
HpyCH4IV ACGT 2 cut(s) 495, 592
HpyCH4V TGCA 8 cut(s) 70, 278, 294, 631, 701, 747, 911, 982
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 275, 914, 1061
HpySE526I ACGT 2 cut(s) 495, 592
Hsp92I GRCGYC 1 cut(s) 812
Hsp92II CATG 9 cut(s) 262, 348, 479, 521, 530, 569, 697, 937, 1045
HspAI GCGC 2 cut(s) 812, 1032
KasI GGCGCC 1 cut(s) 811
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 6 cut(s) 109, 210, 285, 423, 582, 946
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 2 cut(s) 480, 659
Lsp1109I GCAGC 4 cut(s) 262, 685, 895, 994
LweI GCATC 4 cut(s) 387, 734, 756, 920
MaeI CTAG 2 cut(s) 549, 1028
MaeII ACGT 2 cut(s) 495, 592
MaeIII GTNAC 5 cut(s) 101, 231, 551, 805, 936
MalI GATC 6 cut(s) 111, 212, 287, 425, 584, 948
MboI GATC 6 cut(s) 109, 210, 285, 423, 582, 946
MboII GAAGA 6 cut(s) 244, 247, 521, 524, 600, 941
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 1 cut(s) 42
MluI ACGCGT 1 cut(s) 321
Mly113I GGCGCC 1 cut(s) 812
MlyI GAGTC 3 cut(s) 198, 208, 409
MnlI CCTC 8 cut(s) 94, 179, 299, 346, 447, 746, 871, 1061
Mph1103I ATGCAT 1 cut(s) 749
MslI CAYNNNNRTG 2 cut(s) 564, 932
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 3 cut(s) 183, 227, 239
MspR9I CCNGG 1 cut(s) 755
Mva1269I GAATGC 2 cut(s) 631, 910
MvaI CCWGG 1 cut(s) 755
MvnI CGCG 2 cut(s) 323, 437
MwoI GCNNNNNNNGC 4 cut(s) 137, 275, 914, 1061
NarI GGCGCC 1 cut(s) 812
NcoI CCATGG 1 cut(s) 475
NdeI CATATG 1 cut(s) 72
NdeII GATC 6 cut(s) 109, 210, 285, 423, 582, 946
NlaIII CATG 9 cut(s) 262, 348, 479, 521, 530, 569, 697, 937, 1045
NlaIV GGNNCC 2 cut(s) 813, 1039
NmuCI GTSAC 4 cut(s) 101, 551, 805, 936
NruI TCGCGA 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 749
NspI RCATGY 2 cut(s) 521, 937
PaeI GCATGC 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 595
PciI ACATGT 1 cut(s) 933
PciSI GCTCTTC 1 cut(s) 507
PctI GAATGC 2 cut(s) 631, 910
PfeI GAWTC 2 cut(s) 223, 970
PflMI CCANNNNNTGG 1 cut(s) 993
PkrI GCNGC 4 cut(s) 277, 700, 910, 1009
PleI GAGTC 3 cut(s) 197, 207, 409
PluTI GGCGCC 1 cut(s) 815
PpsI GAGTC 3 cut(s) 197, 207, 409
PscI ACATGT 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 753
PspFI CCCAGC 2 cut(s) 365, 917
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 2 cut(s) 813, 1039
PspPI GGNCC 1 cut(s) 1062
PspXI VCTCGAGB 1 cut(s) 85
PstI CTGCAG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 365
RruI TCGCGA 1 cut(s) 437
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 564, 932
SacI GAGCTC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 507
SatI GCNGC 4 cut(s) 276, 699, 909, 1008
Sau3AI GATC 6 cut(s) 109, 210, 285, 423, 582, 946
Sau96I GGNCC 1 cut(s) 1062
SchI GAGTC 3 cut(s) 198, 208, 409
ScrFI CCNGG 1 cut(s) 755
SduI GDGCHC 1 cut(s) 341
SfaNI GCATC 4 cut(s) 387, 734, 756, 920
SfcI CTRYAG 3 cut(s) 276, 360, 859
SfoI GGCGCC 1 cut(s) 813
Sfr274I CTCGAG 1 cut(s) 85
SlaI CTCGAG 1 cut(s) 85
SmiMI CAYNNNNRTG 2 cut(s) 564, 932
SmlI CTYRAG 4 cut(s) 85, 120, 350, 611
SmoI CTYRAG 4 cut(s) 85, 120, 350, 611
SphI GCATGC 1 cut(s) 521
Sse9I AATT 1 cut(s) 42
SsiI CCGC 2 cut(s) 521, 758
SspDI GGCGCC 1 cut(s) 811
SspMI CTAG 2 cut(s) 549, 1028
SstI GAGCTC 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 753
StyI CCWWGG 2 cut(s) 475, 1027
TaaI ACNGT 2 cut(s) 207, 781
TaiI ACGT 2 cut(s) 498, 595
TaqI TCGA 7 cut(s) 86, 161, 327, 426, 572, 770, 776
TaqII GACCGA 1 cut(s) 590
TasI AATT 1 cut(s) 42
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 2 cut(s) 223, 970
TscAI CASTG 1 cut(s) 786
TseFI GTSAC 4 cut(s) 101, 551, 805, 936
TseI GCWGC 4 cut(s) 275, 698, 908, 1007
Tsp45I GTSAC 4 cut(s) 101, 551, 805, 936
TspDTI ATGAA 5 cut(s) 247, 296, 522, 779, 983
TspGWI ACGGA 1 cut(s) 570
TspRI CASTG 1 cut(s) 786
Van91I CCANNNNNTGG 1 cut(s) 993
XapI RAATTY 1 cut(s) 42
XceI RCATGY 2 cut(s) 521, 937
XhoI CTCGAG 1 cut(s) 85
XmaJI CCTAGG 1 cut(s) 1027
XmiI GTMKAC 1 cut(s) 202
XspI CTAG 2 cut(s) 549, 1028
Zsp2I ATGCAT 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.