MD05G1236500.v1.1

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002306
Physical Location & Seq
Forward (+)
36788812 .. 36789453
642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1236500.v1.1.491

Sequence Viewer

Length: 534 bp
ATGCCCAAAAATGACATCTTCTTCCTCATCAAGGCCTTCGCTGCAGGCGTCATTCTAGCAATTGGATTCGTTCACATACTCCCAGATGCATTTGACAACTTGACTTCACCCTGCATCAAAGAAAATCCATGGGGGAAGTTCCCTTACACCGGTTTTGTTACCATGTTATCTGCCACTGGAACACTGATGGTCGATTCATTGGCAACCGGGTACTATCAGAGATCGAAAGTAAAGTCCAACCAAGTGACGATTCATGAACTAGAAAGCGGTGACGATGATCATGCAGGTCATGTACATGGTCACACACATTCCACACAGGGCCATGCTCATGGCTCCGAAGAATTGAAATCATCGGAACTAATCAGGCAGCGTGTCATTTCACTAGTGTTGGAGCTGGGGATCCTAGTGCATTCAGTTATAATCGGAATATCATTGGGTGCTTCCCCAAGTCGTGAAACAATAAAGCCTCTCATGGTTGCTTTGTCTTTCCATCAATTCTTTGAAGGCATGGGACTTGGTGGCTGCATCTGCTAA

Protein Analysis

178

Amino Acids

19.17

Weight (kDa)

6.23

Isoelectric Point (pI)

26.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 3 - 176 4.1e-41 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 275
AciI CCGC 1 cut(s) 267
AclWI GGATC 2 cut(s) 394, 407
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 212, 294
AfiI CCNNNNNNNGG 2 cut(s) 31, 149
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 2 cut(s) 346, 503
AhlI ACTAGT 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AluBI AGCT 1 cut(s) 394
AluI AGCT 1 cut(s) 394
AlwI GGATC 2 cut(s) 394, 407
AoxI GGCC 2 cut(s) 33, 319
ApeKI GCWGC 3 cut(s) 41, 367, 522
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AsiGI ACCGGT 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 99, 281
BamHI GGATCC 1 cut(s) 399
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbvI GCAGC 3 cut(s) 28, 379, 509
BccI CCATC 2 cut(s) 181, 498
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 4 cut(s) 56, 260, 383, 404
BfmI CTRYAG 1 cut(s) 42
BfuAI ACCTGC 1 cut(s) 275
BisI GCNGC 3 cut(s) 42, 368, 523
BlsI GCNGC 3 cut(s) 43, 369, 524
Bme1390I CCNGG 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 334, 401
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 2 cut(s) 76, 123
BpuMI CCSGG 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 149
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 181
BseDI CCNNGG 1 cut(s) 128
BseLI CCNNNNNNNGG 2 cut(s) 31, 149
BseNI ACTGG 1 cut(s) 181
BseXI GCAGC 3 cut(s) 28, 379, 509
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 2 cut(s) 35, 321
BshTI ACCGGT 1 cut(s) 149
BsiSI CCGG 2 cut(s) 150, 207
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 2 cut(s) 31, 149
BsmFI GGGAC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 2 cut(s) 35, 321
Bsp1407I TGTACA 1 cut(s) 292
Bsp143I GATC 3 cut(s) 221, 277, 399
Bsp19I CCATGG 1 cut(s) 128
BspACI CCGC 1 cut(s) 267
BspANI GGCC 2 cut(s) 35, 321
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 334, 401
BspMAI CTGCAG 1 cut(s) 46
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 394, 407
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 292
BsrI ACTGG 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 3 cut(s) 221, 277, 399
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 128
BstACI GRCGYC 1 cut(s) 48
BstAUI TGTACA 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 46
BstDSI CCRYGG 1 cut(s) 128
BstENI CCTNNNNNAGG 1 cut(s) 29
BstKTI GATC 3 cut(s) 224, 280, 402
BstMBI GATC 3 cut(s) 221, 277, 399
BstMWI GCNNNNNNNGC 2 cut(s) 41, 528
BstSCI CCNGG 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 3 cut(s) 28, 379, 509
BstX2I RGATCY 1 cut(s) 399
BstXI CCANNNNNNTGG 1 cut(s) 329
BstYI RGATCY 1 cut(s) 399
BsuRI GGCC 2 cut(s) 35, 321
BtgI CCRYGG 1 cut(s) 128
BtsIMutI CAGTG 2 cut(s) 174, 182
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 46
CciI TCATGA 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 1 cut(s) 37
Csp6I GTAC 2 cut(s) 211, 293
CspAI ACCGGT 1 cut(s) 149
CviJI RGCY 6 cut(s) 35, 321, 333, 394, 466, 522
CviKI_1 RGCY 6 cut(s) 35, 321, 333, 394, 466, 522
CviQI GTAC 2 cut(s) 211, 293
DpnI GATC 3 cut(s) 223, 279, 401
DpnII GATC 3 cut(s) 221, 277, 399
Eco130I CCWWGG 1 cut(s) 128
Eco147I AGGCCT 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 128
FaqI GGGAC 1 cut(s) 525
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 42, 368, 523
Fsp4HI GCNGC 3 cut(s) 42, 368, 523
FspBI CTAG 4 cut(s) 56, 260, 383, 404
GluI GCNGC 3 cut(s) 42, 368, 523
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 2 cut(s) 35, 321
HapII CCGG 2 cut(s) 150, 207
HgaI GACGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 48
HinfI GANTC 3 cut(s) 66, 194, 250
HpaII CCGG 2 cut(s) 150, 207
HphI GGTGA 2 cut(s) 99, 281
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 4 cut(s) 219, 337, 355, 425
Hpy188III TCNNGA 2 cut(s) 254, 452
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 46, 497
HpyCH4V TGCA 6 cut(s) 44, 89, 114, 284, 409, 525
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 528
Hsp92I GRCGYC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 221, 277, 399
LmnI GCTCC 2 cut(s) 338, 391
Lsp1109I GCAGC 3 cut(s) 28, 379, 509
LweI GCATC 2 cut(s) 76, 123
MaeI CTAG 4 cut(s) 56, 260, 383, 404
MaeIII GTNAC 4 cut(s) 157, 244, 269, 299
MalI GATC 3 cut(s) 223, 279, 401
MboI GATC 3 cut(s) 221, 277, 399
MboII GAAGA 3 cut(s) 10, 13, 350
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 1 cut(s) 399
MluCI AATT 3 cut(s) 60, 341, 494
MmeI TCCRAC 2 cut(s) 261, 369
MnlI CCTC 2 cut(s) 35, 477
Mph1103I ATGCAT 1 cut(s) 91
MslI CAYNNNNRTG 2 cut(s) 294, 327
MspI CCGG 2 cut(s) 150, 207
MspR9I CCNGG 1 cut(s) 208
MunI CAATTG 1 cut(s) 60
Mva1269I GAATGC 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 41, 528
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 1 cut(s) 128
NdeII GATC 3 cut(s) 221, 277, 399
NlaIV GGNNCC 2 cut(s) 334, 401
NmuCI GTSAC 3 cut(s) 244, 269, 299
NsiI ATGCAT 1 cut(s) 91
PagI TCATGA 1 cut(s) 253
PceI AGGCCT 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 409
PfeI GAWTC 3 cut(s) 66, 194, 250
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 3 cut(s) 43, 369, 524
PsiI TTATAA 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 334, 401
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 399
RsaI GTAC 2 cut(s) 212, 294
RsaNI GTAC 2 cut(s) 211, 293
RseI CAYNNNNRTG 2 cut(s) 294, 327
SatI GCNGC 3 cut(s) 42, 368, 523
Sau3AI GATC 3 cut(s) 221, 277, 399
Sau96I GGNCC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 208
SetI ASST 2 cut(s) 289, 396
SfaNI GCATC 2 cut(s) 76, 123
SfcI CTRYAG 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 294, 327
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 3 cut(s) 60, 341, 494
SseBI AGGCCT 1 cut(s) 35
SsiI CCGC 1 cut(s) 267
SspMI CTAG 4 cut(s) 56, 260, 383, 404
StuI AGGCCT 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 206
StyI CCWWGG 1 cut(s) 128
TaqI TCGA 2 cut(s) 192, 224
TasI AATT 3 cut(s) 60, 341, 494
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 3 cut(s) 66, 194, 250
TscAI CASTG 2 cut(s) 181, 189
TseFI GTSAC 3 cut(s) 244, 269, 299
TseI GCWGC 3 cut(s) 41, 367, 522
Tsp45I GTSAC 3 cut(s) 244, 269, 299
TspDTI ATGAA 3 cut(s) 186, 242, 270
TspRI CASTG 2 cut(s) 181, 189
XagI CCTNNNNNAGG 1 cut(s) 29
XspI CTAG 4 cut(s) 56, 260, 383, 404
Zsp2I ATGCAT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.