Rroxscaffold_1G00057480

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002306
Physical Location & Seq
Reverse (-)
79419420 .. 79421487
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057480.1

Sequence Viewer

Length: 882 bp
ATGTTGGGCAAGAAAATCCCAACTTTGCGTCCTGAAAATGATATTTTCTTCATGATCAAAGCCTTTGCAGCCGGTGTGATACTAGCCACCGGGTTCATCCATGTCTTGCCGGATGCATTTGAAAGCTTAACAAGCCCCTGCCTCAGCCAAAGGCCATGGGGGGATTTCCCGTTCACCGGCTTCATTGCAATGCTTTCGGCTATAGGGACGATGATGATCGACACCTTTGCTACTAGCTATTATAGAAGATCACATTTCAATAAAGCACTGCCAGTGAAAGATGACCAAGAAATGAATGGCGTGCATGATGGTCATGTTCATGTTCATACTCATGCCACACATGGCCACGCCCATGGATCGGACGCGATAGTCTTGGAGGATTCATCGTCTTCTTTCGAGCTCGTCAGGCGTCGAATAATATCACAGGTCCTGGAGCTTGGGATTGTGGTTCATTCTGTAATCATTGGAGTATCTTTAGGTGCCTCTCAGAGTCCCAAGACAATCAAGCCTCTAGTTGCAGCGCTGACATTCCATCAATTTTTTGAAGGCATGGGCCTTGGCGGTTGCATCTGTCAGGCTAACTTCAAGTCGAAGGCCATTGCAACTATGGTACTTTTCTTCTCCCTCACCACCCCAATTGGAATAGCTGTTGGAATGGGGGTGTCAAGTTTTTACAAGGAGAATAGCAGTGCTGCTCTGATTGTGGAAGGAGTATTCAACTCTGCTTCAGCTGGGATCCTAATCTACATGGCTCTGGTTGACCTTCTAGCAGCTGACTTTATGAATCCTAGAATGCAGAGTAACCTTAGGATCCAGCTTGGGGCTAATCTTTCACTTGTTTTAGGGTCAGGATGTATGTCTCTCTTGGCCAAATGGGCATGA

Protein Analysis

293

Amino Acids

31.53

Weight (kDa)

7.2

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 4 - 290 6.5e-77 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 479
AccII CGCG 1 cut(s) 365
AciI CCGC 1 cut(s) 561
AclWI GGATC 5 cut(s) 364, 730, 743, 805, 818
AcoI YGGCCR 2 cut(s) 343, 867
AcuI CTGAAG 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 1 cut(s) 612
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 3 cut(s) 176, 358, 820
AgsI TTSAA 5 cut(s) 122, 259, 545, 586, 718
AjnI CCWGG 1 cut(s) 429
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 8 cut(s) 126, 237, 400, 436, 647, 731, 773, 817
AluI AGCT 8 cut(s) 126, 237, 400, 436, 647, 731, 773, 817
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 864
AlwI GGATC 5 cut(s) 364, 730, 743, 805, 818
AlwNI CAGNNNCTG 1 cut(s) 430
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 5 cut(s) 152, 343, 553, 594, 867
ApeKI GCWGC 4 cut(s) 68, 518, 692, 770
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 2 cut(s) 427, 553
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 2 cut(s) 166, 619
AvaII GGWCC 1 cut(s) 427
AxyI CCTNAGG 1 cut(s) 806
BalI TGGCCA 2 cut(s) 345, 869
BamHI GGATCC 2 cut(s) 735, 810
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 4 cut(s) 80, 530, 679, 782
BccI CCATC 2 cut(s) 302, 540
BcgI CGANNNNNNTGC 4 cut(s) 177, 209, 211, 243
BciT130I CCWGG 1 cut(s) 431
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 864
BfaI CTAG 5 cut(s) 83, 234, 512, 767, 789
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 875
BisI GCNGC 4 cut(s) 69, 519, 693, 771
BlsI GCNGC 4 cut(s) 70, 520, 694, 772
Bme1390I CCNGG 2 cut(s) 91, 431
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 427, 553
BmiI GGNNCC 3 cut(s) 481, 737, 812
BmrFI CCNGG 2 cut(s) 91, 431
BmsI GCATC 2 cut(s) 103, 576
BpiI GAAGAC 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 91
BsaBI GATNNNNATC 2 cut(s) 215, 740
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 3 cut(s) 155, 352, 556
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 358, 820
Bse118I RCCGGY 2 cut(s) 71, 176
Bse1I ACTGG 1 cut(s) 272
Bse21I CCTNAGG 1 cut(s) 806
Bse3DI GCAATG 3 cut(s) 183, 195, 597
Bse8I GATNNNNATC 2 cut(s) 215, 740
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 3 cut(s) 155, 352, 556
BseGI GGATG 3 cut(s) 96, 118, 857
BseJI GATNNNNATC 2 cut(s) 215, 740
BseLI CCNNNNNNNGG 3 cut(s) 176, 358, 820
BseMI GCAATG 3 cut(s) 183, 195, 597
BseMII CTCAG 2 cut(s) 157, 500
BseNI ACTGG 1 cut(s) 272
BseXI GCAGC 4 cut(s) 80, 530, 679, 782
BseYI CCCAGC 1 cut(s) 731
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 5 cut(s) 154, 345, 555, 596, 869
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 402
BsiSI CCGG 4 cut(s) 72, 90, 110, 177
BslFI GGGAC 2 cut(s) 220, 477
BslI CCNNNNNNNGG 3 cut(s) 176, 358, 820
BsmAI GTCTC 1 cut(s) 864
BsmFI GGGAC 2 cut(s) 220, 477
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 5 cut(s) 154, 345, 555, 596, 869
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 6 cut(s) 54, 216, 248, 356, 735, 810
Bsp19I CCATGG 2 cut(s) 155, 352
BspACI CCGC 1 cut(s) 561
BspANI GGCC 5 cut(s) 154, 345, 555, 596, 869
BspCNI CTCAG 2 cut(s) 156, 499
BspFNI CGCG 1 cut(s) 365
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 481, 737, 812
BspPI GGATC 5 cut(s) 364, 730, 743, 805, 818
BspT107I GGYRCC 1 cut(s) 479
BsrDI GCAATG 3 cut(s) 183, 195, 597
BsrFI RCCGGY 2 cut(s) 71, 176
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 2 cut(s) 71, 176
BssECI CCNNGG 3 cut(s) 155, 352, 556
BssMI GATC 6 cut(s) 54, 216, 248, 356, 735, 810
BssNI GRCGYC 1 cut(s) 409
BssT1I CCWWGG 3 cut(s) 155, 352, 556
Bst2UI CCWGG 1 cut(s) 431
BstACI GRCGYC 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 3 cut(s) 143, 486, 806
BstDSI CCRYGG 2 cut(s) 155, 352
BstF5I GGATG 3 cut(s) 96, 118, 857
BstFNI CGCG 1 cut(s) 365
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 6 cut(s) 57, 219, 251, 359, 738, 813
BstMAI GTCTC 1 cut(s) 864
BstMBI GATC 6 cut(s) 54, 216, 248, 356, 735, 810
BstMWI GCNNNNNNNGC 4 cut(s) 68, 132, 406, 875
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 89, 429
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 4 cut(s) 80, 530, 679, 782
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 2 cut(s) 735, 810
BstXI CCANNNNNNTGG 1 cut(s) 353
BstYI RGATCY 2 cut(s) 735, 810
Bsu36I CCTNAGG 1 cut(s) 806
BsuRI GGCC 5 cut(s) 154, 345, 555, 596, 869
BtgI CCRYGG 2 cut(s) 155, 352
BtsCI GGATG 3 cut(s) 96, 118, 857
BtsI GCAGTG 2 cut(s) 266, 694
BtsIMutI CAGTG 3 cut(s) 266, 279, 694
Cac8I GCNNGC 1 cut(s) 302
CaiI CAGNNNCTG 1 cut(s) 430
CciI TCATGA 1 cut(s) 51
CfoI GCGC 1 cut(s) 523
Cfr10I RCCGGY 2 cut(s) 71, 176
Cfr13I GGNCC 2 cut(s) 427, 553
CseI GACGC 3 cut(s) 17, 371, 398
Csp6I GTAC 1 cut(s) 611
CspCI CAANNNNNGTGG 2 cut(s) 619, 654
CviQI GTAC 1 cut(s) 611
DdeI CTNAG 3 cut(s) 143, 486, 806
DpnI GATC 6 cut(s) 56, 218, 250, 358, 737, 812
DpnII GATC 6 cut(s) 54, 216, 248, 356, 735, 810
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 2 cut(s) 343, 867
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 3 cut(s) 155, 352, 556
Eco24I GRGCYC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 427
Eco47III AGCGCT 1 cut(s) 522
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 711
Eco81I CCTNAGG 1 cut(s) 806
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 3 cut(s) 155, 352, 556
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 3 cut(s) 155, 352, 556
FaqI GGGAC 2 cut(s) 220, 477
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 69, 519, 693, 771
FokI GGATG 3 cut(s) 83, 125, 864
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 4 cut(s) 69, 519, 693, 771
FspBI CTAG 5 cut(s) 83, 234, 512, 767, 789
GlaI GCGC 1 cut(s) 522
GluI GCNGC 4 cut(s) 69, 519, 693, 771
GsaI CCCAGC 1 cut(s) 735
GsuI CTGGAG 1 cut(s) 452
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 5 cut(s) 154, 345, 555, 596, 869
HapII CCGG 4 cut(s) 72, 90, 110, 177
HgaI GACGC 3 cut(s) 17, 371, 398
HhaI GCGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 409
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 3 cut(s) 380, 490, 784
HpaII CCGG 4 cut(s) 72, 90, 110, 177
HphI GGTGA 2 cut(s) 166, 619
Hpy166II GTNNAC 2 cut(s) 174, 760
Hpy188I TCNGA 3 cut(s) 361, 489, 699
Hpy188III TCNNGA 3 cut(s) 32, 52, 849
Hpy8I GTNNAC 2 cut(s) 174, 760
Hpy99I CGWCG 1 cut(s) 414
HpyAV CCTTC 4 cut(s) 539, 586, 701, 773
HpyCH4V TGCA 8 cut(s) 68, 116, 188, 304, 518, 567, 602, 796
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 132, 406, 875
HpyF3I CTNAG 3 cut(s) 143, 486, 806
Hsp92I GRCGYC 1 cut(s) 409
HspAI GCGC 1 cut(s) 521
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 6 cut(s) 54, 216, 248, 356, 735, 810
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 4 cut(s) 80, 530, 679, 782
LweI GCATC 2 cut(s) 103, 576
MaeI CTAG 5 cut(s) 83, 234, 512, 767, 789
MaeIII GTNAC 1 cut(s) 800
MalI GATC 6 cut(s) 56, 218, 250, 358, 737, 812
MboI GATC 6 cut(s) 54, 216, 248, 356, 735, 810
MboII GAAGA 4 cut(s) 40, 258, 381, 610
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 2 cut(s) 735, 810
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 2 cut(s) 345, 869
MluCI AATT 2 cut(s) 536, 636
MluNI TGGCCA 2 cut(s) 345, 869
MlyI GAGTC 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 5 cut(s) 152, 370, 493, 519, 635
Mox20I TGGCCA 2 cut(s) 345, 869
Mph1103I ATGCAT 1 cut(s) 118
MscI TGGCCA 2 cut(s) 345, 869
MseI TTAA 1 cut(s) 128
MslI CAYNNNNRTG 4 cut(s) 188, 318, 330, 351
Msp20I TGGCCA 2 cut(s) 345, 869
MspA1I CMGCKG 2 cut(s) 731, 773
MspI CCGG 4 cut(s) 72, 90, 110, 177
MspR9I CCNGG 2 cut(s) 91, 431
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 4 cut(s) 68, 132, 406, 875
NciI CCSGG 1 cut(s) 91
NcoI CCATGG 2 cut(s) 155, 352
NdeII GATC 6 cut(s) 54, 216, 248, 356, 735, 810
NlaIV GGNNCC 3 cut(s) 481, 737, 812
NsiI ATGCAT 1 cut(s) 118
PagI TCATGA 1 cut(s) 51
PctI GAATGC 1 cut(s) 798
PfeI GAWTC 2 cut(s) 380, 784
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 4 cut(s) 70, 520, 694, 772
PleI GAGTC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 498
PpuMI RGGWCCY 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 731
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 3 cut(s) 481, 737, 812
PspPI GGNCC 2 cut(s) 427, 553
PspPPI RGGWCCY 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 430
PsuI RGATCY 2 cut(s) 735, 810
PvuII CAGCTG 2 cut(s) 731, 773
RsaI GTAC 1 cut(s) 612
RsaNI GTAC 1 cut(s) 611
RseI CAYNNNNRTG 4 cut(s) 188, 318, 330, 351
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 4 cut(s) 69, 519, 693, 771
Sau3AI GATC 6 cut(s) 54, 216, 248, 356, 735, 810
Sau96I GGNCC 2 cut(s) 427, 553
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 2 cut(s) 91, 431
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 2 cut(s) 103, 576
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 427
SmiMI CAYNNNNRTG 4 cut(s) 188, 318, 330, 351
Sse9I AATT 2 cut(s) 536, 636
SsiI CCGC 1 cut(s) 561
SspMI CTAG 5 cut(s) 83, 234, 512, 767, 789
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 2 cut(s) 89, 429
StyI CCWWGG 3 cut(s) 155, 352, 556
TaqI TCGA 4 cut(s) 219, 396, 412, 590
TasI AATT 2 cut(s) 536, 636
TfiI GAWTC 2 cut(s) 380, 784
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 3 cut(s) 273, 279, 694
TseI GCWGC 4 cut(s) 68, 518, 692, 770
TspDTI ATGAA 9 cut(s) 40, 85, 172, 308, 308, 314, 372, 440, 797
TspRI CASTG 3 cut(s) 273, 279, 694
VpaK11BI GGWCC 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 2 cut(s) 293, 604
XspI CTAG 5 cut(s) 83, 234, 512, 767, 789
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.