Rw5G015370

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002306
Physical Location & Seq
Reverse (-)
19107396 .. 19108380
985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015370.1

Sequence Viewer

Length: 651 bp
ATGTCTGCCATCGGAACATTGATGGTTGATGCAATGGCAACTGGGTACTACAAAAGGTCAAGCTTGAAGTCATCCCAGGTGAGAATGGATGAAGAGAGCAGAGCCGGTCATGGTGCCATACAGGGTACTAATGCTCAAGACTCTGAAGAATTGAGATCATCATTAGAATTATTCAAGCACAAGGTCATTGCGCAAGTGTTGGAGCTGGGAATTCTAGTCCATTCAGTTATAATTGGAATATCAGTGGGTGCATCCCAGGACATAGATACAATAAAGCCTCTCCTGGTGGCTTTGTCTTTCCACCAGTTCTTTGAGGGCATTAGTCTTGGTGGCTGCATCTCTCAGGCGCAATTCAAGTCTCTGTCTGAAGCCATAATGGTGGCATTTTTCTCCCTTACAACCCCCATTGGAATTGCAATTGGTATCGGAATATCAAATGTTTACAATGAGACTAGCCCAACTGCTCTAATTGTTCAAGGGACTTTGAATTCAGCTGCAGCTGGAATTTTAATTTACATGGCTCTTGTTGACCTACTTGCAGCAGATTTCATGAGCCCTAGAATGCAAGGAAATATGAGGATTCAATTGGGGTCCTATATTTCGCTTCTTCTAGGGACTGGCTGTATGTCTCTCTTGGCAAAATGGGCTTGA

Protein Analysis

216

Amino Acids

22.95

Weight (kDa)

5.76

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 1 - 213 5.8e-52 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 113
AcsI RAATTY 3 cut(s) 210, 487, 504
AcuI CTGAAG 2 cut(s) 165, 387
AfaI GTAC 2 cut(s) 47, 127
AgsI TTSAA 6 cut(s) 67, 175, 355, 476, 487, 584
AjnI CCWGG 3 cut(s) 75, 255, 282
AluBI AGCT 4 cut(s) 63, 205, 494, 500
AluI AGCT 4 cut(s) 63, 205, 494, 500
Alw26I GTCTC 3 cut(s) 363, 443, 633
ApeKI GCWGC 4 cut(s) 333, 494, 497, 539
ApoI RAATTY 3 cut(s) 210, 487, 504
AspLEI GCGC 2 cut(s) 193, 349
AspS9I GGNCC 1 cut(s) 591
AsuHPI GGTGA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 591
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 320, 481, 509, 551
BccI CCATC 2 cut(s) 16, 17
BciT130I CCWGG 3 cut(s) 77, 257, 284
BcoDI GTCTC 3 cut(s) 363, 443, 633
BfaI CTAG 4 cut(s) 215, 453, 558, 611
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 4 cut(s) 334, 495, 498, 540
BlsI GCNGC 4 cut(s) 335, 496, 499, 541
Bme1390I CCNGG 3 cut(s) 77, 257, 284
Bme18I GGWCC 1 cut(s) 591
BmgT120I GGNCC 1 cut(s) 591
BmiI GGNNCC 2 cut(s) 115, 592
BmrFI CCNGG 3 cut(s) 77, 257, 284
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 3 cut(s) 19, 260, 345
BmuI ACTGGG 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 75, 255
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 3 cut(s) 46, 304, 622
Bse3DI GCAATG 2 cut(s) 39, 186
BseBI CCWGG 3 cut(s) 77, 257, 284
BseDI CCNNGG 2 cut(s) 75, 255
BseGI GGATG 3 cut(s) 71, 94, 251
BseMI GCAATG 2 cut(s) 39, 186
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 3 cut(s) 46, 304, 622
BseXI GCAGC 4 cut(s) 320, 481, 509, 551
BseYI CCCAGC 1 cut(s) 205
BshNI GGYRCC 1 cut(s) 113
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 2 cut(s) 493, 628
BsmAI GTCTC 3 cut(s) 363, 443, 633
BsmFI GGGAC 2 cut(s) 493, 628
BsmI GAATGC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 557
Bsp143I GATC 1 cut(s) 155
BspCNI CTCAG 1 cut(s) 355
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 115, 592
BspMAI CTGCAG 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 39, 186
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 3 cut(s) 46, 304, 622
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 75, 255
BssMI GATC 1 cut(s) 155
Bst2UI CCWGG 3 cut(s) 77, 257, 284
Bst6I CTCTTC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 3 cut(s) 71, 94, 251
BstHHI GCGC 2 cut(s) 193, 349
BstKTI GATC 1 cut(s) 158
BstMAI GTCTC 3 cut(s) 363, 443, 633
BstMBI GATC 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 3 cut(s) 77, 257, 284
BstSCI CCNGG 3 cut(s) 75, 255, 282
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 320, 481, 509, 551
BstXI CCANNNNNNTGG 1 cut(s) 379
BtsCI GGATG 3 cut(s) 71, 94, 251
BtsIMutI CAGTG 1 cut(s) 249
CciI TCATGA 1 cut(s) 549
CfoI GCGC 2 cut(s) 193, 349
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 591
Csp6I GTAC 2 cut(s) 46, 126
CviAII CATG 3 cut(s) 110, 517, 550
CviQI GTAC 2 cut(s) 46, 126
DdeI CTNAG 1 cut(s) 342
DpnI GATC 1 cut(s) 157
DpnII GATC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 557
Eco47I GGWCC 1 cut(s) 591
Eco57I CTGAAG 2 cut(s) 165, 387
EcoO109I RGGNCCY 1 cut(s) 591
EcoRI GAATTC 2 cut(s) 210, 487
EcoRII CCWGG 3 cut(s) 75, 255, 282
EcoT38I GRGCYC 1 cut(s) 557
FaeI CATG 3 cut(s) 113, 520, 553
FaqI GGGAC 2 cut(s) 493, 628
FatI CATG 3 cut(s) 109, 516, 549
Fnu4HI GCNGC 4 cut(s) 334, 495, 498, 540
FokI GGATG 3 cut(s) 58, 101, 238
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 4 cut(s) 334, 495, 498, 540
FspBI CTAG 4 cut(s) 215, 453, 558, 611
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 2 cut(s) 192, 348
GluI GCNGC 4 cut(s) 334, 495, 498, 540
GsaI CCCAGC 1 cut(s) 209
HapII CCGG 1 cut(s) 105
HhaI GCGC 2 cut(s) 193, 349
Hin1II CATG 3 cut(s) 113, 520, 553
Hin6I GCGC 2 cut(s) 191, 347
HinP1I GCGC 2 cut(s) 191, 347
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 2 cut(s) 140, 580
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 442, 529
Hpy188I TCNGA 4 cut(s) 14, 145, 367, 428
Hpy188III TCNNGA 2 cut(s) 137, 550
Hpy8I GTNNAC 2 cut(s) 442, 529
HpyCH4V TGCA 7 cut(s) 32, 251, 336, 416, 497, 539, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 3 cut(s) 113, 520, 553
HspAI GCGC 2 cut(s) 191, 347
Kzo9I GATC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 4 cut(s) 320, 481, 509, 551
LweI GCATC 3 cut(s) 19, 260, 345
MaeI CTAG 4 cut(s) 215, 453, 558, 611
MalI GATC 1 cut(s) 157
MboI GATC 1 cut(s) 155
MboII GAAGA 3 cut(s) 104, 158, 599
MfeI CAATTG 2 cut(s) 417, 584
MhlI GDGCHC 1 cut(s) 557
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 3 cut(s) 288, 307, 570
MseI TTAA 1 cut(s) 509
MslI CAYNNNNRTG 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 494, 500
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 3 cut(s) 77, 257, 284
MunI CAATTG 2 cut(s) 417, 584
Mva1269I GAATGC 1 cut(s) 567
MvaI CCWGG 3 cut(s) 77, 257, 284
MwoI GCNNNNNNNGC 1 cut(s) 644
NdeII GATC 1 cut(s) 155
NlaIII CATG 3 cut(s) 113, 520, 553
NlaIV GGNNCC 2 cut(s) 115, 592
NsbI TGCGCA 1 cut(s) 192
PagI TCATGA 1 cut(s) 549
PctI GAATGC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 580
PkrI GCNGC 4 cut(s) 335, 496, 499, 541
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 591
PsiI TTATAA 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 75, 255, 282
PspFI CCCAGC 1 cut(s) 205
PspGI CCWGG 3 cut(s) 75, 255, 282
PspN4I GGNNCC 2 cut(s) 115, 592
PspPI GGNCC 1 cut(s) 591
PspPPI RGGWCCY 1 cut(s) 591
PstI CTGCAG 1 cut(s) 499
PvuII CAGCTG 2 cut(s) 494, 500
RsaI GTAC 2 cut(s) 47, 127
RsaNI GTAC 2 cut(s) 46, 126
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 1 cut(s) 509
SatI GCNGC 4 cut(s) 334, 495, 498, 540
Sau3AI GATC 1 cut(s) 155
Sau96I GGNCC 1 cut(s) 591
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 3 cut(s) 77, 257, 284
SduI GDGCHC 1 cut(s) 557
SetI ASST 8 cut(s) 59, 65, 81, 186, 207, 496, 502, 534
SfaNI GCATC 3 cut(s) 19, 260, 345
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
SspMI CTAG 4 cut(s) 215, 453, 558, 611
StyD4I CCNGG 3 cut(s) 75, 255, 282
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 1 cut(s) 509
Tru9I TTAA 1 cut(s) 509
TscAI CASTG 1 cut(s) 249
TseI GCWGC 4 cut(s) 333, 494, 497, 539
TspDTI ATGAA 2 cut(s) 105, 538
TspRI CASTG 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 591
XapI RAATTY 3 cut(s) 210, 487, 504
XspI CTAG 4 cut(s) 215, 453, 558, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.